FastQCFastQC Report
Thu 26 May 2016
SRR1926690_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926690_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708036
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10390.1467439508725545No Hit
GTCCTAAAGTGTGTATTTCTCATTT8530.12047410018699616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGA500.002030200616.444622
TAGGACC2900.016.3013364
CTTAAGG505.018592E-415.3664613
GCTATAT708.401208E-515.2599671
GTCCTAA3100.014.6446461
GTGTACT658.297921E-414.3795831
TCTTACA1151.2266719E-714.299672
ATTGGAC702.807055E-514.1120573
TGTAGGA7300.014.0792992
AGGACCT5500.013.6660845
CTTACAC652.369373E-413.5089773
GTCCTAG904.7197973E-513.352471
TAGGCTG550.001508674113.2864715
CTGTAGG7400.013.1720311
TCCTAGA1151.9122617E-613.1080312
GTCCTAC8500.012.88120651
GCCATAT2300.012.7719281
ATAGCCC704.4320966E-412.544053
GTAGGAC7600.012.4202593
GGACCTG5300.012.4005746
Produced by FastQC (version 0.11.5)