FastQCFastQC Report
Thu 26 May 2016
SRR1926690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708036
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGACGTGAAATATGGCGAGG19490.27526848917286695No Hit
GTCCTACAGTGGACATTTCTAAATT19320.27286748131450944No Hit
CTGTAGGACGTGGAATATGGCAAGA19010.26848917286691637No Hit
GTCCTAAAGTGTGTATTTCTCATTT17940.25337694693490165No Hit
GTCCTACAGTGTGCATTTCTCATTT15610.22046901569976665No Hit
CTGTAGGACCTGGAATATGGCGAGA13300.1878435559773797No Hit
GTATCAACGCAGAGTACTTTTTTTT12180.17202515126349507No Hit
CTGAAGGACCTGGAATATGGCGAGA9430.1331853182606534No Hit
GGTATCAACGCAGAGTACTTTTTTT7330.10352580942211978No Hit
CCCATGTACTCTGCGTTGATACCAC7110.10041862278189245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4450.014.1457314
TTGTAGT500.001454124113.3527134
GTTCTAG905.160582E-712.712351
TGTAGGA12400.012.0810522
GTAGGAC12100.012.0617223
CTGTAGG12100.012.0557191
TTAGAAC657.7430054E-411.7403183
GGATAGG657.775418E-411.7344771
GTATAAG657.775418E-411.7344771
GGACATG2950.011.6391696
AGGACCT8200.011.6312825
GGACCTG8000.011.5644036
TGGCGAA752.1262665E-411.36758318
ATTATAC855.0958006E-511.22236253
GGCGAGG4750.011.16815119
ATAGCAC600.005693871511.1288433
TAATACT600.005699905611.127264
GTATTGG600.00571501411.1233061
GATTTAG600.00571501411.1233061
TAGGACA3100.011.0759834