FastQCFastQC Report
Thu 26 May 2016
SRR1926689_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926689_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657276
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCA452.6850315E-416.6818374
GTCCTAC4150.016.3722741
TGTAGGA4500.015.1802352
TCTACAC803.9935367E-715.0616433
TATTACA656.9725316E-414.713152
GTCCTAA2550.014.6286681
ATTTATA751.2433722E-414.5730242
CTGTAGG4600.014.1913991
TAGGACC1850.013.9122654
AATCCAC450.006224850713.8973125
TCCTACA5150.013.7948542
TTAAGAC755.471408E-513.1447073
TATAGTG902.6374564E-612.73920255
GTGTTGG957.998552E-512.6213511
TAAGACT851.534594E-512.6165144
TCCTAAA3150.012.57790852
CTAGAAC704.5070378E-412.5187693
AGGACCT3000.012.5075815
GTAGGAC4800.012.3231633
GGACCTG2750.012.1234646
Produced by FastQC (version 0.11.5)