FastQCFastQC Report
Thu 26 May 2016
SRR1926689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657276
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA11910.1812024172493747No Hit
GTCCTACAGTGGACATTTCTAAATT11680.17770312623616258No Hit
GTCCTAAAGTGTGTATTTCTCATTT11620.17679026771097683No Hit
CTTTAGGACGTGAAATATGGCGAGG11060.16827025480924301No Hit
GTCCTACAGTGTGCATTTCTCATTT8760.13327734467712193No Hit
CTGTAGGACCTGGAATATGGCGAGA7880.11988875297439736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTATA400.00514033814.3055531
TGTAGGA7450.013.1945282
TAGGACC3450.012.9974624
ACTGTTC1550.012.8718458
ATAAGGG603.9365602E-412.7228693
TCTATAC603.9365602E-412.7228693
TAGGCTC603.9416557E-412.7209195
TAATCCC603.9416557E-412.7209195
GTCCTAA3700.012.6301281
AATCCCG604.190437E-412.62898419
GATATAC1750.012.53438951
CTGTAGG7350.012.4565351
GTAGGAC7200.012.325283
CTAGGGT550.002968596812.1426964
TAGACAG802.7379692E-511.9258625
CTATACT802.7379692E-511.9258624
TTAATCC907.0961014E-611.6608434
TATACTG907.0961014E-611.6608435
ACTACAC1001.8239607E-611.45058253
CTAGGCT855.0853563E-511.2243414
Produced by FastQC (version 0.11.5)