FastQCFastQC Report
Thu 26 May 2016
SRR1926688_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926688_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663618
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12330.18579966185365676No Hit
GTCCTAAAGTGTGTATTTCTCATTT10980.16545663318354836No Hit
CTTTAGGACGTGAAATATGGCGAGG8900.1341133001214554No Hit
CTGTAGGACGTGGAATATGGCAAGA8760.13200365270381453No Hit
GTCCTACAGTGTGCATTTCTCATTT7980.12024990280552969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC808.3664054E-716.700281
TTTAGAA2000.015.742742
TGTAGGA9350.015.37305452
CTGTAGG8800.015.3338941
CTCTAGG708.357441E-515.2688271
TATTAAT902.2165132E-615.2103772
TAGGACC4000.015.0192194
CATACTA507.336283E-414.6163455
GTACAAT550.00453705514.574791
GTTTAGA550.00453705514.574791
GTCCTAA4550.014.0943021
GGACCTG6000.014.0869816
AGGACCT6650.013.8156645
GTAGGAC9850.013.7983323
ATAGCAC652.3969726E-413.490183
TAGCACA808.187997E-613.4100174
GTATTGA700.001445767213.3602241
GTACACA802.599758E-413.3602241
TAGACAG550.001507478213.2875865
TTAGCAC500.00895845613.1529263
Produced by FastQC (version 0.11.5)