FastQCFastQC Report
Thu 26 May 2016
SRR1926688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663618
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGACGTGAAATATGGCGAGG26590.4006823202505056No Hit
GTCCTAAAGTGTGTATTTCTCATTT24180.36436624684683055No Hit
GTCCTACAGTGGACATTTCTAAATT23850.3593935065052485No Hit
CTGTAGGACGTGGAATATGGCAAGA23430.35306456425232585No Hit
GTCCTACAGTGTGCATTTCTCATTT17620.2655141964202297No Hit
CTGTAGGACCTGGAATATGGCGAGA14030.21141680906786733No Hit
CTGAAGGACCTGGAATATGGCGAGA12530.188813443878858No Hit
GTATCAACGCAGAGTACTTTTTTTT10520.1585249345255855No Hit
GTACATGGGAAGCAGTGGTATCAAC10060.15159323586762263No Hit
CCCATGTACTCTGCGTTGATACCAC9820.14797669743738115No Hit
GTCCTTCAGTGTGCATTTCTCATTT8560.12898987067861328No Hit
GAATATGGCAAGAAAACTGAAAATC8370.12612677775467213No Hit
GGAATATGGCGAGAAAACTGAAAAT7460.11241406954000643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACTG1150.015.7482495
AGGACGG400.005159053514.2977525
TAGGACC4150.013.7809684
ATAGGAC1204.9112714E-1113.505483
AGTACTG655.2750056E-513.1979265
CCAGTAC957.032395E-813.0455423
TCCAACG802.0541265E-613.02825918
GGCGAGG7100.012.54449619
TAGAAAT3200.012.5105334
CTAAGAC701.05408464E-412.2570743
TGTAGAA3150.012.1094252
GAGTGCC550.0030579512.0947579
CCAACGA550.003126104412.059216519
ATTTAGA3100.011.9916631
CCTACAC802.7599364E-511.91663
CTATTAG657.790918E-411.7314891
TTTAGAA3600.011.6553222
AAATGTC2700.011.6165617
ACGAAAT907.5862754E-611.59115612
CGAAATC1001.9659037E-611.37954913
Produced by FastQC (version 0.11.5)