##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926688_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 663618 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.69281876019035 31.0 28.0 31.0 23.0 34.0 2 29.171939278319755 31.0 30.0 33.0 25.0 34.0 3 29.496400037370897 31.0 30.0 33.0 25.0 34.0 4 33.24521336069847 35.0 33.0 37.0 28.0 37.0 5 32.945229032365006 35.0 33.0 37.0 26.0 37.0 6 33.07603319982279 35.0 33.0 37.0 27.0 37.0 7 32.905450123414376 35.0 33.0 37.0 26.0 37.0 8 32.897361132458734 35.0 33.0 37.0 26.0 37.0 9 34.155598552179114 38.0 34.0 39.0 25.0 39.0 10 34.05657019550404 38.0 34.0 39.0 25.0 39.0 11 34.316688215208146 38.0 35.0 39.0 27.0 39.0 12 34.11135321826713 38.0 34.0 39.0 26.0 39.0 13 34.28792920023266 38.0 35.0 39.0 27.0 39.0 14 34.93759361560416 39.0 34.0 40.0 25.0 41.0 15 35.11166514470674 39.0 34.0 40.0 26.0 41.0 16 35.100661826532736 39.0 35.0 41.0 26.0 41.0 17 35.02472054706171 39.0 34.0 41.0 25.0 41.0 18 35.00100811008743 39.0 34.0 41.0 25.0 41.0 19 35.027457061140595 39.0 34.0 41.0 25.0 41.0 20 35.02026165655543 39.0 34.0 41.0 25.0 41.0 21 34.93228031789373 39.0 34.0 41.0 25.0 41.0 22 34.87883842813185 39.0 34.0 41.0 25.0 41.0 23 34.5417243052479 39.0 34.0 41.0 19.0 41.0 24 34.65636857348655 39.0 34.0 41.0 23.0 41.0 25 34.457584031777316 39.0 34.0 41.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.559854942576397 1101 2 0.7673273489268837 1101 3 0.6424001349125774 1101 4 0.40365638648204083 1101 5 0.6393680691655632 1101 6 0.1305849802621566 1101 7 0.5770642659073957 1101 8 0.8065404536897915 1101 9 1.258566020312685 1101 10 1.022756020525371 1101 11 0.8673162347156307 1101 12 1.0211469000726865 1101 13 0.7360867517807748 1101 14 0.9918341718625712 1101 15 0.8081667615118633 1101 16 0.8967925577611879 1101 17 0.7866042515053664 1101 18 0.9435197774538366 1101 19 1.0881919123332793 1101 20 1.0572028406710103 1101 21 1.078459838828742 1101 22 0.9641379150056011 1101 23 1.1940482267229342 1101 24 1.0926525529700726 1101 25 1.200016309370234 1102 1 0.5887672232781505 1102 2 0.6311402144239615 1102 3 0.4464118308190095 1102 4 0.346790889406023 1102 5 0.5064440925573805 1102 6 0.030163927630582066 1102 7 0.3919765466091505 1102 8 0.3726340209412484 1102 9 0.6149636811313997 1102 10 0.7564870146774183 1102 11 0.5195267610314218 1102 12 0.496831110599004 1102 13 0.39281189797960536 1102 14 0.8244423589970751 1102 15 0.7992076971843787 1102 16 0.6664650723810723 1102 17 0.6330253041369502 1102 18 0.8500460932433143 1102 19 0.8000047778303525 1102 20 0.688173600904932 1102 21 0.6958048680685067 1102 22 0.8790618916137944 1102 23 1.0664224957287871 1102 24 0.8833776991689035 1102 25 0.5975601690193528 1103 1 0.3067816117673594 1103 2 0.07105068604666442 1103 3 0.0019545966063816422 1103 4 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0.24841249743437288 2202 18 0.14931838528400476 2202 19 0.1926518953248646 2202 20 0.17365375251075932 2202 21 0.42982681101684506 2202 22 0.3919372157709802 2202 23 0.5857267047852375 2202 24 0.23500506800991872 2202 25 0.08536387937243717 2203 1 0.04314210785802075 2203 2 0.15920177182623974 2203 3 0.0022800979781365527 2203 4 -0.05867048332405034 2203 5 -0.08533183849833392 2203 6 -0.2576883346039551 2203 7 0.09576232684922559 2203 8 0.2150815432558062 2203 9 0.5658605724825847 2203 10 0.5838825209870535 2203 11 0.27137902326595764 2203 12 0.37696407458792436 2203 13 0.226668469232294 2203 14 0.5608092411146473 2203 15 0.5921926156208244 2203 16 0.5216771376319187 2203 17 0.5415904010898558 2203 18 0.49550500367729455 2203 19 0.47323346357981677 2203 20 0.6282093041982506 2203 21 0.42596676403649525 2203 22 0.7027344062336738 2203 23 0.5844452719676241 2203 24 0.5463459971067337 2203 25 0.3914290517525032 2204 1 0.012267223278151107 2204 2 0.1957403709184824 2204 3 -0.18470945243607417 2204 4 -0.42256983046878105 2204 5 -0.11568814531429439 2204 6 -0.49370461697036205 2204 7 -0.18895068343779542 2204 8 -0.07230807608535628 2204 9 0.3512766701767873 2204 10 0.30103005067115873 2204 11 -0.1534028164333705 2204 12 -0.06925339644325135 2204 13 -0.22434381407047255 2204 14 0.2853046438171063 2204 15 0.27686888654275066 2204 16 0.2178328345094016 2204 17 0.03395722901957754 2204 18 0.1340343561541104 2204 19 0.23978568549858892 2204 20 0.1936094381506308 2204 21 0.26385025148008623 2204 22 0.27664718112865927 2204 23 0.4885132625519475 2204 24 0.21197550824558675 2204 25 0.1746611079864877 2205 1 0.679229274987911 2205 2 0.8388547913862432 2205 3 0.770880740095258 2205 4 0.5366809830025332 2205 5 0.573247910553853 2205 6 0.42924760847572685 2205 7 0.528979604713129 2205 8 0.5506905495298895 2205 9 0.8228198575747356 2205 10 0.834466071298678 2205 11 0.45377863759522086 2205 12 0.6178919946690655 2205 13 0.7216785461123081 2205 14 1.041132563797369 2205 15 1.0268299750084893 2205 16 0.8570920763658734 2205 17 1.0216780703309993 2205 18 0.7887972747834837 2205 19 0.9062859380564703 2205 20 0.8842429179283755 2205 21 0.8031039135693874 2205 22 0.64603511003655 2205 23 1.1039263415262113 2205 24 0.9678606942574035 2205 25 0.9678242779990569 2206 1 0.33246611121656144 2206 2 0.5896886413795741 2206 3 0.4790764064296944 2206 4 0.523886602741257 2206 5 0.7271721602315537 2206 6 0.4215594223554149 2206 7 0.5880985882401717 2206 8 0.652516921806189 2206 9 0.890247019217135 2206 10 0.7465670450927746 2206 11 0.6300554180031952 2206 12 0.5906822654321928 2206 13 0.5011476075129195 2206 14 0.6500695807440522 2206 15 0.5626742878126478 2206 16 0.5042880930064868 2206 17 0.4943038898227172 2206 18 0.5758310946690344 2206 19 0.4504448500668303 2206 20 0.7537856149720383 2206 21 0.6600462899865747 2206 22 0.786356635459434 2206 23 0.8910422086838281 2206 24 0.7765756841513181 2206 25 0.9169328522243703 2207 1 -0.9056712977880572 2207 2 -0.05136900502764874 2207 3 0.1382555300642565 2207 4 0.4106724218412907 2207 5 0.023742267774728987 2207 6 0.08962165190687443 2207 7 0.29899861596999955 2207 8 0.21487114714742006 2207 9 0.470443952460343 2207 10 0.32827109798686394 2207 11 0.21326364839169543 2207 12 0.15635303665231248 2207 13 -0.10029978636165993 2207 14 -5.21835423983736 2207 15 -2.0661344256779515 2207 16 -0.9220252304755263 2207 17 -0.9444444579725371 2207 18 -0.10006683309661213 2207 19 0.26083978308496114 2207 20 0.2405580472601443 2207 21 0.34181308436735236 2207 22 0.4806378923780983 2207 23 0.6872162284124599 2207 24 0.2846838996083818 2207 25 0.19345584113170844 2208 1 0.23269356435100974 2208 2 0.7327368695258194 2208 3 0.701651044412106 2208 4 0.6329668523986385 2208 5 0.9184074410139189 2208 6 0.7999274717992506 2208 7 0.8178639231770717 2208 8 0.8172570971800184 2208 9 0.8919119953383259 2208 10 0.9224750940297923 2208 11 0.7122953759233326 2208 12 0.6814203820161353 2208 13 0.652290592045965 2208 14 1.0525451968228907 2208 15 0.9059305866070275 2208 16 0.6591041614300934 2208 17 0.7181024325063134 2208 18 0.8928237820610363 2208 19 0.6384426392305826 2208 20 0.7002351612643309 2208 21 0.9261815249495697 2208 22 0.8304014521511078 2208 23 1.1168187238223695 2208 24 0.9745489472784996 2208 25 0.9415260083574921 2209 1 -0.23745300948818837 2209 2 -0.14391360035769551 2209 3 0.35172885061409787 2209 4 0.311302179568159 2209 5 0.4808835633621129 2209 6 0.33527643136081053 2209 7 0.6356053141412303 2209 8 0.6472847719608481 2209 9 0.3539876540747997 2209 10 0.11558141435910585 2209 11 0.41670133578522695 2209 12 0.5401757838308754 2209 13 0.57647846517348 2209 14 0.8991297160049285 2209 15 0.8545519725250728 2209 16 0.6553288941258231 2209 17 0.8709459248471845 2209 18 0.8859470183402962 2209 19 0.9102246127055125 2209 20 0.6335304098176948 2209 21 0.828433138236619 2209 22 0.9939593351629767 2209 23 0.9885384810068132 2209 24 0.8721221535606247 2209 25 1.0197386232121346 2210 1 -0.17894335186099397 2210 2 -0.13991152748492297 2210 3 0.4487585634514595 2210 4 0.7842767686061833 2210 5 1.0502865992570278 2210 6 0.8471979412360184 2210 7 1.0399666517421124 2210 8 1.0354227243013838 2210 9 1.3628787501893314 2210 10 1.2535993626469093 2210 11 1.0530109910870777 2210 12 0.8993765651444576 2210 13 0.9478108672642875 2210 14 1.313196018036443 2210 15 1.0664293009774113 2210 16 1.2821146291734848 2210 17 1.1301953721641596 2210 18 1.3823701501387973 2210 19 1.1125547675232 2210 20 1.3757068930096494 2210 21 1.4623692877757861 2210 22 1.4851612525702933 2210 23 1.5520633945125368 2210 24 1.5161577445203775 2210 25 1.5414315357478614 2211 1 -0.6752327767218489 2211 2 0.061128734309161104 2211 3 0.392076447465179 2211 4 0.7447064285614147 2211 5 1.0624178522949776 2211 6 0.8873176791680919 2211 7 1.062579252636212 2211 8 1.0674195887969304 2211 9 1.457339244887038 2211 10 1.6266952967602393 2211 11 1.0734824805886163 2211 12 1.2095912582004829 2211 13 1.0315777856384827 2211 14 1.5982496570700562 2211 15 1.1810805959133646 2211 16 1.420615953889886 2211 17 1.3778420323042297 2211 18 1.4812908656910366 2211 19 1.5346378241608178 2211 20 1.4791682708516305 2211 21 1.4035974059938852 2211 22 1.3508052290471682 2211 23 1.6038009295131204 2211 24 1.5647118525104347 2211 25 1.6056905503231675 2212 1 0.8190638012249174 2212 2 0.08852339989079638 2212 3 0.5538803563787909 2212 4 0.45779553663880534 2212 5 0.49358393201660533 2212 6 0.46685340798546093 2212 7 0.66143155294629 2212 8 0.7189145448111276 2212 9 0.9696159836240099 2212 10 0.6440721435325116 2212 11 0.48061421350290345 2212 12 0.6587094375951992 2212 13 0.5649073774895328 2212 14 0.6357638630105953 2212 15 0.6663450017893666 2212 16 0.015080197011407392 2212 17 0.549765481576749 2212 18 0.6890904531926125 2212 19 0.5127466451729816 2212 20 0.657082768627788 2212 21 0.7384191477474289 2212 22 0.7438257276932134 2212 23 1.0241529562273044 2212 24 0.8427659543442303 2212 25 0.3353041487405193 2213 1 0.6434695133544857 2213 2 0.4253191796487279 2213 3 -0.19034728707751114 2213 4 -0.9862659384141637 2213 5 -1.7071097666453383 2213 6 -0.383192306466114 2213 7 -0.08652218112622023 2213 8 0.07032443654769338 2213 9 -0.6628615945259355 2213 10 -0.6148929683590509 2213 11 -0.40114499469377307 2213 12 -0.4349398817674057 2213 13 -0.6848386533350705 2213 14 -8.10377986322515 2213 15 -3.5246107930615906 2213 16 -2.2366613059056135 2213 17 -1.1524810571862076 2213 18 -5.394490802430987 2213 19 -3.0579511169957705 2213 20 -5.465667790104398 2213 21 -2.15161582743616 2213 22 -1.9516616028730596 2213 23 -4.578105918181308 2213 24 -2.6080438445121814 2213 25 -2.001778253372507 2214 1 -0.2788913133072164 2214 2 -0.44363756335381765 2214 3 -1.990997383273129 2214 4 -2.1889516580194552 2214 5 -0.9084068559521086 2214 6 -0.7591775357840547 2214 7 -0.7374949981062926 2214 8 -0.8575909113080726 2214 9 -5.523494313448545 2214 10 -1.8678408986017132 2214 11 -1.3937984422206142 2214 12 -1.728949377205872 2214 13 -1.9081366115054905 2214 14 -2.812113196558485 2214 15 -2.4953099179917757 2214 16 -1.9138086403560521 2214 17 -2.1364381104971955 2214 18 -1.71546610187864 2214 19 -4.808951861736041 2214 20 -2.625360274433824 2214 21 -4.674043370413877 2214 22 -7.015119680201927 2214 23 -6.824265851154685 2214 24 -6.01254642007229 2214 25 -4.023903245614793 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19835.0 3 9.0 4 374.0 5 2162.0 6 2806.0 7 2199.0 8 1622.0 9 1328.0 10 1180.0 11 1122.0 12 1137.0 13 980.0 14 1074.0 15 1097.0 16 1182.0 17 1194.0 18 1335.0 19 1631.0 20 2192.0 21 2765.0 22 3474.0 23 4543.0 24 5370.0 25 6239.0 26 7174.0 27 8063.0 28 9503.0 29 11462.0 30 14331.0 31 17922.0 32 23380.0 33 30588.0 34 40816.0 35 55041.0 36 75528.0 37 113663.0 38 189297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.98265525432676 20.559254930055427 9.636439048301346 29.821650767316466 2 12.416923301873851 21.845421944458263 42.045966719576484 23.691688034091406 3 17.64331699846399 26.571471208473834 29.521093386111392 26.264118406950786 4 11.570538183846194 18.75255300067075 38.93478637696233 30.74212243852073 5 12.145341966912651 39.838813726908796 35.92958689046448 12.086257415714078 6 30.284878435430024 39.29501171551788 18.490702462149574 11.92940738690252 7 26.846533629151253 32.00104512934986 23.145084191814462 18.00733704968442 8 23.282544208891924 40.721872149153285 20.47897245666713 15.516611185287669 9 26.224422133171814 15.43832990709119 20.321820052050686 38.01542790768631 10 14.746920911277634 30.260524364438712 34.768081168576714 20.22447355570694 11 33.28435826842254 22.38657751697928 26.411273005094916 17.91779120950327 12 24.177132403246564 24.93376974475357 32.59432725431514 18.294770597684728 13 29.69705463082677 23.322905647526138 25.644572630639917 21.335467091007175 14 19.510341343507537 24.57612932900025 27.801663361558703 28.111865965933514 15 21.42482564903165 34.87772828373548 22.769151481907883 20.928294585324984 16 20.038094893656776 28.652694520543815 31.04462587740316 20.264584708396246 17 20.02793881189223 30.826726623095784 29.04054141619939 20.1047931488126 18 20.033182440129956 28.068697596759513 32.62397453903231 19.274145424078213 19 23.43288979973177 27.127831105619265 27.428459486746732 22.01081960790223 20 23.51924462112011 28.758133493265664 28.54581126980443 19.176810615809792 21 21.49067686530504 27.30396101371572 27.27337112615993 23.93199099481931 22 22.721957511701007 30.57783242769184 26.851140264429237 19.849069796177922 23 20.611556648553837 30.17368425811235 28.641778854702554 20.57298023863126 24 23.20257498312277 27.770427476130777 29.34648953611727 19.680508004629182 25 21.471690038546274 29.105599908381024 28.949938066779385 20.47277198629332 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 116.0 1 116.0 2 124.0 3 132.0 4 132.0 5 132.0 6 248.0 7 364.0 8 364.0 9 364.0 10 642.5 11 921.0 12 921.0 13 921.0 14 1674.0 15 2427.0 16 2427.0 17 2427.0 18 4833.0 19 7239.0 20 7239.0 21 7239.0 22 12342.0 23 17445.0 24 17445.0 25 17445.0 26 27024.0 27 36603.0 28 36603.0 29 36603.0 30 50120.5 31 63638.0 32 63638.0 33 63638.0 34 70708.0 35 77778.0 36 77778.0 37 77778.0 38 81992.0 39 86206.0 40 86206.0 41 86206.0 42 87552.0 43 88898.0 44 88898.0 45 88898.0 46 89284.5 47 89671.0 48 89671.0 49 89671.0 50 82157.5 51 74644.0 52 74644.0 53 74644.0 54 62094.5 55 49545.0 56 49545.0 57 49545.0 58 40924.5 59 32304.0 60 32304.0 61 32304.0 62 25754.5 63 19205.0 64 19205.0 65 19205.0 66 14454.0 67 9703.0 68 9703.0 69 9703.0 70 6961.5 71 4220.0 72 4220.0 73 4220.0 74 2923.0 75 1626.0 76 1626.0 77 1626.0 78 1131.5 79 637.0 80 637.0 81 637.0 82 417.0 83 197.0 84 197.0 85 197.0 86 137.5 87 78.0 88 78.0 89 78.0 90 49.0 91 20.0 92 20.0 93 20.0 94 10.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08935863704721693 2 0.03390504778351401 3 0.03179540036587314 4 0.027576105530591394 5 0.02441163440413009 6 0.38109273708669744 7 0.22588296278883332 8 0.2311570813329355 9 0.23673257807955783 10 0.01657580113860685 11 0.002260336518900934 12 0.003616538430241494 13 0.0 14 0.07579661793381132 15 0.002109647417640872 16 0.001808269215120747 17 0.0037672275315015565 18 0.002109647417640872 19 0.0012055128100804983 20 6.027564050402491E-4 21 0.0 22 0.0 23 0.0 24 3.0137820252012457E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 663618.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.35142550581004 #Duplication Level Percentage of deduplicated Percentage of total 1 92.12818989238288 72.18375007338159 2 5.756376503201381 9.020406095479567 3 0.9648889251657535 2.2680126822451694 4 0.3698046742486793 1.1589889354438296 5 0.17972675812259306 0.7040923850221543 6 0.10826108062206741 0.5089445996123045 7 0.08070674907754834 0.44264421867159415 8 0.062320357396567154 0.39063110720420713 9 0.045678043917254045 0.32210458697104644 >10 0.2470143875131442 3.592789849784459 >50 0.028419769661559915 1.5606771047116448 >100 0.023779605534235776 3.812522344477931 >500 0.0030932820200572277 1.5682404476963028 >1k 0.0017399711362821903 2.4661955692981268 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTAGGACGTGAAATATGGCGAGG 2659 0.4006823202505056 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2418 0.36436624684683055 No Hit GTCCTACAGTGGACATTTCTAAATT 2385 0.3593935065052485 No Hit CTGTAGGACGTGGAATATGGCAAGA 2343 0.35306456425232585 No Hit GTCCTACAGTGTGCATTTCTCATTT 1762 0.2655141964202297 No Hit CTGTAGGACCTGGAATATGGCGAGA 1403 0.21141680906786733 No Hit CTGAAGGACCTGGAATATGGCGAGA 1253 0.188813443878858 No Hit GTATCAACGCAGAGTACTTTTTTTT 1052 0.1585249345255855 No Hit GTACATGGGAAGCAGTGGTATCAAC 1006 0.15159323586762263 No Hit CCCATGTACTCTGCGTTGATACCAC 982 0.14797669743738115 No Hit GTCCTTCAGTGTGCATTTCTCATTT 856 0.12898987067861328 No Hit GAATATGGCAAGAAAACTGAAAATC 837 0.12612677775467213 No Hit GGAATATGGCGAGAAAACTGAAAAT 746 0.11241406954000643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGACTG 115 0.0 15.748249 5 AGGACGG 40 0.0051590535 14.297752 5 TAGGACC 415 0.0 13.780968 4 ATAGGAC 120 4.9112714E-11 13.50548 3 AGTACTG 65 5.2750056E-5 13.197926 5 CCAGTAC 95 7.032395E-8 13.045542 3 TCCAACG 80 2.0541265E-6 13.028259 18 GGCGAGG 710 0.0 12.544496 19 TAGAAAT 320 0.0 12.510533 4 CTAAGAC 70 1.05408464E-4 12.257074 3 TGTAGAA 315 0.0 12.109425 2 GAGTGCC 55 0.00305795 12.094757 9 CCAACGA 55 0.0031261044 12.0592165 19 ATTTAGA 310 0.0 11.991663 1 CCTACAC 80 2.7599364E-5 11.9166 3 CTATTAG 65 7.790918E-4 11.731489 1 TTTAGAA 360 0.0 11.655322 2 AAATGTC 270 0.0 11.616561 7 ACGAAAT 90 7.5862754E-6 11.591156 12 CGAAATC 100 1.9659037E-6 11.379549 13 >>END_MODULE