FastQCFastQC Report
Thu 26 May 2016
SRR1926687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711870
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATG250.00717354418.43565211
GGACCGG300.00992998417.4236666
AGGACCG503.82312E-516.7332615
GTATAGA500.00237356616.0644971
GTACTAT801.5175658E-515.0604651
TGTAGGA4250.014.54232
TAAGACC450.00502334414.3543074
TAGGACC2500.014.2107644
GTGTAGC954.90021E-614.0916631
CTGTAGG4000.013.3870811
ATACAAG600.00797029313.387081
GTTCTAG700.001424338413.3870791
GATATAC1409.113137E-913.3870791
GTTTTAG700.001424338413.3870791
CGCAGGG450.00942838413.0521168
CTAGGAC600.001962576512.8299743
TAACACT851.4751115E-512.66556554
TTTACAC704.3609904E-412.5681373
TAAGACT600.002302706212.56001954
CTTATAC902.1322443E-512.2190223
Produced by FastQC (version 0.11.5)