##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926687_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 711870 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.548184359503843 27.0 10.0 31.0 2.0 31.0 2 16.550368747102702 20.0 2.0 31.0 2.0 31.0 3 20.170685658898396 25.0 15.0 31.0 2.0 31.0 4 22.26523101128015 28.0 7.0 35.0 2.0 37.0 5 22.83212524758734 29.0 7.0 35.0 2.0 37.0 6 23.649691657184597 31.0 2.0 35.0 2.0 37.0 7 23.439106859398485 30.0 7.0 35.0 2.0 37.0 8 23.8758270470732 31.0 11.0 35.0 2.0 37.0 9 26.70111256268701 32.0 17.0 37.0 2.0 39.0 10 27.462414485790944 32.0 25.0 38.0 2.0 39.0 11 24.466224170143423 30.0 8.0 37.0 2.0 39.0 12 25.650815457878544 31.0 17.0 37.0 2.0 39.0 13 22.179479399328528 28.0 2.0 37.0 2.0 39.0 14 25.669963616952533 31.0 17.0 38.0 2.0 40.0 15 26.815630662901935 31.0 17.0 38.0 2.0 40.0 16 27.20073468470367 32.0 19.0 39.0 2.0 40.0 17 26.50705606360712 31.0 17.0 38.0 2.0 40.0 18 26.6126385435543 31.0 17.0 38.0 2.0 40.0 19 27.029178080267464 31.0 19.0 39.0 2.0 40.0 20 27.304212847851435 31.0 23.0 39.0 2.0 40.0 21 25.112049952940847 30.0 10.0 38.0 2.0 40.0 22 26.072421930970542 31.0 11.0 38.0 2.0 40.0 23 26.066982735611838 31.0 8.0 38.0 2.0 40.0 24 25.068322867939372 31.0 2.0 39.0 2.0 40.0 25 24.871101465155153 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.0553953594981955 1101 2 10.896832967681945 1101 3 7.216670045403273 1101 4 8.967720942301007 1101 5 8.414313198884514 1101 6 7.94278477565107 1101 7 7.6078451813148575 1101 8 6.280028757137558 1101 9 6.122340720104983 1101 10 5.66806547217438 1101 11 8.470538570653755 1101 12 6.3229944584473685 1101 13 10.675638674073603 1101 14 7.9435730346308375 1101 15 7.120453691689356 1101 16 6.485667297346051 1101 17 2.7127294178187427 1101 18 5.344895467379704 1101 19 5.636213841183874 1101 20 3.9016119619874026 1101 21 0.9356563596280871 1101 22 4.922537244540287 1101 23 6.3398851069936555 1101 24 7.5571778383943204 1101 25 8.093506763283234 1102 1 6.280009906792667 1102 2 11.374992677651967 1102 3 7.28728724996029 1102 4 8.590985422050341 1102 5 8.50910467198095 1102 6 7.3944887852779075 1102 7 7.4297598719121964 1102 8 7.586126889328366 1102 9 6.870353863045242 1102 10 5.942034947758263 1102 11 8.740150497572774 1102 12 6.370428474052698 1102 13 10.77240882643121 1102 14 8.230899813197631 1102 15 6.134965630231285 1102 16 6.558820663432339 1102 17 7.4234037764879375 1102 18 7.400493361892366 1102 19 5.943404666459472 1102 20 6.514745715276796 1102 21 5.118516807409538 1102 22 6.352606627071253 1102 23 6.654135286383237 1102 24 8.42036083247081 1102 25 8.136249158452554 1103 1 4.512869769373854 1103 2 8.86510862255054 1103 3 6.041812975568522 1103 4 7.410941420110209 1103 5 6.9093093686075875 1103 6 6.402728643571432 1103 7 6.490856900534826 1103 8 6.270621297919639 1103 9 5.114802517595827 1103 10 4.114611867465758 1103 11 5.221462493265168 1103 12 4.028392803849304 1103 13 8.40387797699719 1103 14 6.402480314739975 1103 15 5.313945551586183 1103 16 5.044325592461583 1103 17 2.791628364441607 1103 18 1.5455231297960204 1103 19 3.6398503951445598 1103 20 4.049643498727132 1103 21 6.2280373372561435 1103 22 1.9177879730406033 1103 23 4.176410111190986 1103 24 5.6150541044758775 1103 25 1.7453213357366408 1104 1 5.525250563676124 1104 2 10.119506476024927 1104 3 6.780427391090228 1104 4 7.318861534459888 1104 5 7.36566142734074 1104 6 6.9310718901365505 1104 7 6.995974926521395 1104 8 7.043301930903265 1104 9 5.721923034693408 1104 10 4.776456579067531 1104 11 7.736191049957149 1104 12 4.769202716715288 1104 13 9.33220435094917 1104 14 6.986704147100276 1104 15 6.086970495618488 1104 16 5.660395453310539 1104 17 5.950117758015196 1104 18 3.8352643066539294 1104 19 4.470975428066733 1104 20 3.802939145318593 1104 21 3.1811102104838334 1104 22 5.163971744604627 1104 23 5.274563451694039 1104 24 6.483908632371929 1104 25 5.412491208657055 1105 1 3.6461809959314593 1105 2 9.64682363941533 1105 3 6.312360386228676 1105 4 5.380297409539153 1105 5 2.2018918754489647 1105 6 4.6681861365960735 1105 7 5.430818247172418 1105 8 3.1641088427612267 1105 9 3.771872833890196 1105 10 3.4572977067360995 1105 11 6.559797317131107 1105 12 3.991442333075465 1105 13 4.658631771156312 1105 14 3.7483883840480665 1105 15 4.263197773149667 1105 16 4.479500822154332 1105 17 5.266292324703002 1105 18 2.8244724550369327 1105 19 2.4396996671282416 1105 20 1.759936212534825 1105 21 2.93656954783323 1105 22 4.135861936952967 1105 23 1.769148370315893 1105 24 4.742115142623462 1105 25 5.502128045478024 1106 1 6.789527375891332 1106 2 11.612287400735433 1106 3 7.970809273118572 1106 4 9.381499357920447 1106 5 8.678241116734998 1106 6 7.956198870613957 1106 7 7.563949979821842 1106 8 7.452641527910082 1106 9 6.3226478162660875 1106 10 5.654077837585152 1106 11 8.084873954099951 1106 12 5.128261571905288 1106 13 5.004667223529747 1106 14 5.820364294042292 1106 15 5.927219387805273 1106 16 5.767563318366346 1106 17 6.3067611100907826 1106 18 6.254980507899695 1106 19 6.136737859612239 1106 20 5.921364770292463 1106 21 5.877955424939749 1106 22 6.404615425888856 1106 23 6.974862090964969 1106 24 7.6846997782270385 1106 25 6.898884571183707 1107 1 3.8548227227432434 1107 2 -14.173256681869809 1107 3 -5.844938978293852 1107 4 1.5411201755435044 1107 5 4.464346946329144 1107 6 4.610412320568241 1107 7 4.675468447017142 1107 8 5.360219298905612 1107 9 3.466105612259039 1107 10 2.868535018752663 1107 11 5.473358962279722 1107 12 3.003208730407035 1107 13 4.62237217429632 1107 14 3.8873480412927996 1107 15 3.623922773389289 1107 16 3.4845041289375516 1107 17 3.8055241925083543 1107 18 -1.4242992427326655 1107 19 1.5724904963161137 1107 20 1.8862495805654191 1107 21 -1.3484368261112358 1107 22 1.845620302156025 1107 23 -0.9984432371641461 1107 24 2.387789007534181 1107 25 3.066635238929635 1108 1 4.865845446947837 1108 2 10.525243712763181 1108 3 0.030463738865744716 1108 4 5.8664453160824515 1108 5 7.1726845964513615 1108 6 7.341056624070834 1108 7 7.141917735892889 1108 8 6.802048763454259 1108 9 5.292414352767597 1108 10 4.464424556677301 1108 11 6.7184156895949165 1108 12 1.9757483020183244 1108 13 2.9069399208822873 1108 14 4.5719078763820455 1108 15 4.5451952183368896 1108 16 4.688811624955118 1108 17 0.03937296613217711 1108 18 3.4889336145922805 1108 19 4.560601902747912 1108 20 4.767899240653616 1108 21 4.840307625637742 1108 22 1.4927192002603427 1108 23 4.469557584124562 1108 24 5.164468803518027 1108 25 5.6601707926308045 1109 1 3.201486481834465 1109 2 -14.173256681869809 1109 3 -5.891482396068023 1109 4 1.8948408625389703 1109 5 0.49143787671290085 1109 6 2.4979355912675523 1109 7 3.627723635222079 1109 8 3.939335638268126 1109 9 3.2321810719184505 1109 10 2.3169780524845756 1109 11 1.279068309730949 1109 12 1.5090824366369198 1109 13 2.966614972999217 1109 14 3.325140060805861 1109 15 3.291104323207769 1109 16 3.297167427662661 1109 17 3.807091052609554 1109 18 3.8159755231469354 1109 19 2.2822801413092293 1109 20 1.1266253039257705 1109 21 0.3414581377257946 1109 22 2.7364347867277026 1109 23 3.0314401771370854 1109 24 4.038466028400073 1109 25 4.540347930092626 1110 1 2.912218205996208 1110 2 9.10266168547713 1110 3 6.214299571541357 1110 4 7.650835889991576 1110 5 8.231951706610342 1110 6 3.8687598600613633 1110 7 6.266021919002597 1110 8 7.0583798041578305 1110 9 5.262688340815856 1110 10 4.779576208498252 1110 11 2.4453116192374935 1110 12 5.298666331108414 1110 13 9.891799544289661 1110 14 7.465568943018646 1110 15 6.483019099270479 1110 16 6.2306720376284765 1110 17 6.576204626973308 1110 18 6.709242555848341 1110 19 5.437634262653198 1110 20 5.840241105576382 1110 21 4.153240312524648 1110 22 5.820805794013864 1110 23 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18 0.751822496915679 2208 19 3.3464195949679443 2208 20 4.651307350256069 2208 21 7.203682307504462 2208 22 6.181866581965409 2208 23 2.081141645172387 2208 24 3.462714269459301 2208 25 4.891577669796536 2209 1 2.865242993306712 2209 2 4.948774440161312 2209 3 4.914695031936816 2209 4 0.10486052687335601 2209 5 4.707369313456521 2209 6 5.415314206615712 2209 7 5.81816419114487 2209 8 4.328710245916845 2209 9 3.9509873262576996 2209 10 2.9783045843694858 2209 11 -1.0615736705049414 2209 12 2.59866532825027 2209 13 2.2296036192365953 2209 14 3.359080785101913 2209 15 3.850533849642371 2209 16 3.319322023421318 2209 17 0.9586519351349025 2209 18 2.9353750334093895 2209 19 3.700276125295062 2209 20 3.68577420825234 2209 21 5.789563834562458 2209 22 4.382108841031197 2209 23 -0.1483575255032541 2209 24 -3.842015976447417 2209 25 -0.5365051112274202 2210 1 -1.9504781563446087 2210 2 -14.173256681869809 2210 3 -7.6666877669929825 2210 4 -2.2696384125315845 2210 5 -0.7330507599089415 2210 6 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-6.139247943799791 2211 19 -9.077759826016752 2211 20 -7.058738486260356 2211 21 -4.834514789516167 2211 22 -6.418673445957985 2211 23 -8.619980559195255 2211 24 -22.70688084131228 2211 25 -22.532410107132417 2212 1 -1.0581641081340472 2212 2 -14.173256681869809 2212 3 -7.931503288132186 2212 4 -19.94018451817169 2212 5 -20.389272783185575 2212 6 -21.216954425652922 2212 7 -9.981119672797986 2212 8 -9.582219606554194 2212 9 -5.106741712870807 2212 10 -5.646875229472862 2212 11 -5.550052889719325 2212 12 -2.958047032713644 2212 13 -6.269395661870748 2212 14 -3.8652804631743045 2212 15 -3.78655375733009 2212 16 -4.0121984817041465 2212 17 -4.0533472610981285 2212 18 -3.172434926482058 2212 19 -3.9118279668798905 2212 20 -3.8794205057466264 2212 21 -1.6086511934134222 2212 22 -3.039757102535365 2212 23 -3.2668667263862368 2212 24 -2.1355788041112156 2212 25 -2.5279814091696196 2213 1 -19.248598120534403 2213 2 -14.173256681869809 2213 3 -8.481142481868028 2213 4 -5.682471269532851 2213 5 -20.389272783185575 2213 6 -10.643451703329873 2213 7 -21.025406652425975 2213 8 -10.722237996501452 2213 9 -8.296112355506228 2213 10 -7.147687023473303 2213 11 -6.246737715923068 2213 12 -4.864956766043331 2213 13 -19.722894333261358 2213 14 -12.513948076130214 2213 15 -6.87917061145955 2213 16 -6.016668975001622 2213 17 -4.630445989716197 2213 18 -4.657986600787087 2213 19 -5.817278882278096 2213 20 -5.698393658674149 2213 21 -3.8463115227285094 2213 22 -4.733373990422596 2213 23 -4.486949706200701 2213 24 -3.3765723113667256 2213 25 -4.2810489456079175 2214 1 -19.248598120534403 2214 2 -14.173256681869809 2214 3 -12.568710974959123 2214 4 -19.94018451817169 2214 5 -20.389272783185575 2214 6 -21.216954425652922 2214 7 -16.94007587582003 2214 8 -15.47053404326033 2214 9 -14.581228930790772 2214 10 -14.314427352657754 2214 11 -14.903457042589654 2214 12 -12.900188620268109 2214 13 -19.722894333261358 2214 14 -17.302890172171058 2214 15 -24.45085845174993 2214 16 -18.833571216720244 2214 17 -24.16583618935322 2214 18 -18.43274895216426 2214 19 -15.589019620376524 2214 20 -14.367319502559493 2214 21 -11.777947196035822 2214 22 -12.859950266799723 2214 23 -12.848760272885869 2214 24 -11.635931656269136 2214 25 -11.4116047380049 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 120570.0 3 2.0 4 476.0 5 1959.0 6 2790.0 7 2682.0 8 2392.0 9 2201.0 10 2079.0 11 1945.0 12 1887.0 13 2064.0 14 3229.0 15 7451.0 16 10977.0 17 11696.0 18 11946.0 19 13875.0 20 16437.0 21 18810.0 22 19615.0 23 21187.0 24 22376.0 25 22974.0 26 18724.0 27 14609.0 28 15769.0 29 18164.0 30 17765.0 31 20868.0 32 26729.0 33 34203.0 34 41379.0 35 48031.0 36 47648.0 37 49975.0 38 36386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70104887965409 18.932759643044413 12.59831715337231 25.767874323929192 2 13.570613673759624 20.9372886010328 41.679894800059834 23.81220292514774 3 18.63832510459125 25.42307496777782 29.360759815428967 26.57784011220196 4 11.312307755403355 18.04650781282041 38.81376368781528 31.827420743960953 5 12.124205399765483 38.05969539029652 36.29706069462002 13.51903851531798 6 30.200632876625676 38.989328321811605 18.52321019260956 12.286828608953156 7 26.51586182915546 31.46907424540662 23.125081497947804 18.88998242749011 8 23.865161069977923 37.883768586170305 21.518485539685056 16.732584804166716 9 26.34769780742895 15.404952727037491 20.370758406543256 37.8765910589903 10 14.14850403706533 29.493064528794875 35.38196848875975 20.976462945380042 11 32.68444492299778 22.2757603763822 25.698313996787707 19.341480703832314 12 23.254186805382844 24.75586332541269 32.57033443503223 19.419615434172243 13 28.171305977794297 21.920215927351418 25.506757042784162 24.401721052070123 14 20.237533562660307 22.682067602846857 28.114429013007587 28.96596982148525 15 22.13703471056636 31.884629835680784 23.74570052906522 22.23263492468763 16 21.360839646015645 28.08999690837019 28.739384315712012 21.809779129902157 17 20.882689521234703 28.60872478646363 29.151322554865324 21.357263137436345 18 20.858964066022292 27.549335291411786 31.162447179024177 20.42925346354174 19 22.84404285426665 26.957264249414912 27.493442349074076 22.70525054724436 20 22.95769508736685 27.9685217300651 28.397642792639854 20.676140389928193 21 21.97705168382975 26.443482695896485 26.893958378169618 24.685507242104148 22 22.355392465306775 28.60789506801359 27.88800352366461 21.14870894301502 23 21.720352986675103 28.135850648665752 28.3693000035158 21.77449636114334 24 22.405559786157298 27.39212094799255 28.728870470692787 21.473448795157367 25 22.234457579874384 27.556838158564613 28.545370675082342 21.663333586478664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 730.0 1 730.0 2 1601.5 3 2473.0 4 2473.0 5 2473.0 6 3547.5 7 4622.0 8 4622.0 9 4622.0 10 5436.5 11 6251.0 12 6251.0 13 6251.0 14 7789.5 15 9328.0 16 9328.0 17 9328.0 18 13167.5 19 17007.0 20 17007.0 21 17007.0 22 23766.0 23 30525.0 24 30525.0 25 30525.0 26 39708.5 27 48892.0 28 48892.0 29 48892.0 30 58582.5 31 68273.0 32 68273.0 33 68273.0 34 74520.5 35 80768.0 36 80768.0 37 80768.0 38 83492.5 39 86217.0 40 86217.0 41 86217.0 42 86611.0 43 87005.0 44 87005.0 45 87005.0 46 84414.0 47 81823.0 48 81823.0 49 81823.0 50 75256.5 51 68690.0 52 68690.0 53 68690.0 54 59136.5 55 49583.0 56 49583.0 57 49583.0 58 41185.5 59 32788.0 60 32788.0 61 32788.0 62 26180.5 63 19573.0 64 19573.0 65 19573.0 66 14808.0 67 10043.0 68 10043.0 69 10043.0 70 7325.5 71 4608.0 72 4608.0 73 4608.0 74 3190.5 75 1773.0 76 1773.0 77 1773.0 78 1209.5 79 646.0 80 646.0 81 646.0 82 423.5 83 201.0 84 201.0 85 201.0 86 121.5 87 42.0 88 42.0 89 42.0 90 24.5 91 7.0 92 7.0 93 7.0 94 3.5 95 0.0 96 0.0 97 0.0 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.004382822706393 2 37.07994437186565 3 6.051104836557237 4 14.36989899841263 5 14.103558233947208 6 15.609732114009581 7 9.507634820964501 8 7.86744770814896 9 0.1105538932670291 10 1.6133563712475592 11 6.066838046272494 12 6.239341452793347 13 17.039768497057047 14 1.7463862783935267 15 3.1664489302822147 16 3.218705662550746 17 3.14790621883209 18 1.6665964291232949 19 1.6215039262786743 20 0.2875525025636703 21 4.702544003820922 22 1.7697051427929256 23 0.11167769396097602 24 9.425456895219634 25 0.1146276707825867 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 711870.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.7583685521227 #Duplication Level Percentage of deduplicated Percentage of total 1 96.6553823000625 89.65595573935514 2 2.5199627829292544 4.6749527311316905 3 0.3802953786937425 1.0582673668662972 4 0.15669051540182824 0.5813742630505938 5 0.07653834309549219 0.35497859186102404 6 0.05156034637363775 0.2869592166960596 7 0.03188094557621434 0.20700571496840592 8 0.02012716461484667 0.14935703626025523 9 0.014591404768504965 0.12181274110891659 >10 0.08102008370884475 1.3896661924468603 >50 0.006208328361558513 0.4127531411262952 >100 0.005432006071228778 0.9186160852145233 >500 3.1040034224462886E-4 0.18830117991389528 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 1.4047508674336606E-4 0.0 0.0 0.0 13 0.0 1.4047508674336606E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAATG 25 0.007173544 18.435652 11 GGACCGG 30 0.009929984 17.423666 6 AGGACCG 50 3.82312E-5 16.733261 5 GTATAGA 50 0.002373566 16.064497 1 GTACTAT 80 1.5175658E-5 15.060465 1 TGTAGGA 425 0.0 14.5423 2 TAAGACC 45 0.005023344 14.354307 4 TAGGACC 250 0.0 14.210764 4 GTGTAGC 95 4.90021E-6 14.091663 1 CTGTAGG 400 0.0 13.387081 1 ATACAAG 60 0.007970293 13.38708 1 GTTCTAG 70 0.0014243384 13.387079 1 GATATAC 140 9.113137E-9 13.387079 1 GTTTTAG 70 0.0014243384 13.387079 1 CGCAGGG 45 0.009428384 13.052116 8 CTAGGAC 60 0.0019625765 12.829974 3 TAACACT 85 1.4751115E-5 12.6655655 4 TTTACAC 70 4.3609904E-4 12.568137 3 TAAGACT 60 0.0023027062 12.5600195 4 CTTATAC 90 2.1322443E-5 12.219022 3 >>END_MODULE