FastQCFastQC Report
Thu 26 May 2016
SRR1926687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711870
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA13180.18514616432775646No Hit
CTTTAGGACGTGAAATATGGCGAGG12850.18051048646522538No Hit
GTCCTACAGTGGACATTTCTAAATT12740.17896526051104839No Hit
GTCCTAAAGTGTGTATTTCTCATTT12130.17039628021970304No Hit
GTATCAACGCAGAGTACTTTTTTTT11130.15634877154536644No Hit
GTCCTACAGTGTGCATTTCTCATTT9710.13640130922780846No Hit
CTGTAGGACCTGGAATATGGCGAGA8740.12277522581370195No Hit
CCCATGTACTCTGCGTTGATACCAC8030.11280149465492295No Hit
GGTATCAACGCAGAGTACTTTTTTT7490.10521583997078118No Hit
GTACATGGGAAGCAGTGGTATCAAC7280.1022658631491705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT753.09592E-916.53541
ACCGACT350.002166596516.288018
AGGTATC500.001495763213.3028117
TAGGACC3150.013.0233054
CCTATAC751.4109242E-512.7222383
ACTTACC604.082303E-412.6684528
TAGTACT701.04704006E-412.2661374
TCCTAAC657.7020214E-411.7477612
GGACCTG5400.011.6595766
TGGCGAG8850.011.34416418
TGTAGGA8400.011.2495292
TGGACTG855.0887073E-511.2239165
AGGACCT5800.011.1852125
ATAATAC600.005682069311.1319583
GTCTAAA600.00569105811.1295951
AGGACGT8700.011.0755535
CTAGGAC1303.049354E-811.0096283
GGCGAGG4250.010.9198619
GGACGTG8650.010.9182166
TATTACA700.001431996610.9086352