FastQCFastQC Report
Thu 26 May 2016
SRR1926686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679773
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11460.16858568963462803No Hit
GTCCTAAAGTGTGTATTTCTCATTT9610.14137072228523345No Hit
CTGTAGGACGTGGAATATGGCAAGA8740.1285723322344371No Hit
CTTTAGGACGTGAAATATGGCGAGG7370.10841854560272325No Hit
GTCCTACAGTGTGCATTTCTCATTT7140.10503506317550122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAG402.0282776E-523.344911
TAGGACC3050.016.5026784
CCAATAC450.004516861414.58569053
TATACTT507.4653595E-414.5828845
CAAGGGC450.005201659614.2789114
TGTAGGA7100.014.2473172
GTCCTAA3750.014.2292791
GTTCTAA751.5235749E-414.2292781
GCAGAAC550.001059801213.9227063
GTCCTAC7300.013.7054261
ATACACC550.001244403313.629874
AGGACCT5600.013.0204325
CTGTAGG7500.012.9842171
CTAGAGT1004.5140587E-712.851024
TTAAAAC600.002041781312.7624793
TCCTAAA4800.012.530582
GTCTTAT750.002457265712.4506181
GTCTTAG750.002457265712.4506181
GTAGGAC7700.012.3599523
TCCTACA8550.012.3107452
Produced by FastQC (version 0.11.5)