##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926686_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679773 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.872002859778192 27.0 10.0 31.0 2.0 31.0 2 16.79551409073323 22.0 2.0 31.0 2.0 31.0 3 20.41440304336889 25.0 15.0 31.0 2.0 31.0 4 22.580579105083608 28.0 7.0 35.0 2.0 37.0 5 23.147199138535953 30.0 7.0 35.0 2.0 37.0 6 24.015590498592914 31.0 10.0 35.0 2.0 37.0 7 23.790110816404887 31.0 10.0 35.0 2.0 37.0 8 24.229152967240534 31.0 17.0 35.0 2.0 37.0 9 27.102092904543134 32.0 18.0 37.0 2.0 39.0 10 27.85937511492807 32.0 26.0 38.0 2.0 39.0 11 24.862124562170017 30.0 10.0 37.0 2.0 39.0 12 26.06085119591393 31.0 17.0 37.0 2.0 39.0 13 22.57785466619004 29.0 2.0 37.0 2.0 39.0 14 26.075585526344824 31.0 17.0 38.0 2.0 40.0 15 27.244881747289167 32.0 20.0 38.0 2.0 40.0 16 27.62547644581353 32.0 23.0 39.0 2.0 40.0 17 26.936756829117957 31.0 17.0 39.0 2.0 40.0 18 27.04658466870558 31.0 18.0 38.0 2.0 40.0 19 27.45883699411421 31.0 23.0 39.0 2.0 40.0 20 27.73467760561246 32.0 25.0 39.0 2.0 40.0 21 25.509353857831954 31.0 10.0 38.0 2.0 40.0 22 26.48691695610152 31.0 17.0 39.0 2.0 40.0 23 26.49973741234206 31.0 16.0 39.0 2.0 40.0 24 25.4956684069535 31.0 2.0 39.0 2.0 40.0 25 25.302214121478787 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.773473902651766 1101 2 10.713758345563296 1101 3 6.931780699705207 1101 4 8.345658980803922 1101 5 8.01843807175602 1101 6 7.45217323982218 1101 7 7.325457526908913 1101 8 5.619794957064428 1101 9 5.4565767958806894 1101 10 5.192951644617967 1101 11 7.925494643686658 1101 12 5.5191456529939416 1101 13 10.083476213053995 1101 14 7.484112196678929 1101 15 6.644733999172011 1101 16 5.999741908214514 1101 17 2.130462659649261 1101 18 4.350250553098483 1101 19 5.164351968531992 1101 20 3.106211169795287 1101 21 0.48804421535590237 1101 22 4.28712035725156 1101 23 5.403770035671229 1101 24 6.653754392827935 1101 25 7.26988198613471 1102 1 5.968660445294429 1102 2 11.324100337995631 1102 3 7.016403054713791 1102 4 8.485009389150935 1102 5 8.265362803312371 1102 6 7.01010032505539 1102 7 6.772187855757814 1102 8 7.272938114465482 1102 9 6.1626255717081655 1102 10 5.432190738235246 1102 11 8.28799344947792 1102 12 5.643119719122705 1102 13 10.189545833252193 1102 14 7.873100723589886 1102 15 5.40264110854995 1102 16 5.898643091422056 1102 17 6.805468522128525 1102 18 6.634598167720807 1102 19 5.269821589309199 1102 20 5.723407920389494 1102 21 4.5230026858423 1102 22 6.027522537804341 1102 23 6.311490993238461 1102 24 7.9054870811438 1102 25 7.676911750159068 1103 1 5.002571453707745 1103 2 9.3597414092126 1103 3 6.262009761669592 1103 4 7.848076695703842 1103 5 6.9508350156598055 1103 6 6.701855864811286 1103 7 6.426388590442016 1103 8 6.258025210092928 1103 9 5.454991373343429 1103 10 4.636909899273288 1103 11 5.453928394376607 1103 12 4.015444639136927 1103 13 8.763257173458378 1103 14 6.697985188573888 1103 15 5.790255162347798 1103 16 5.619631952661539 1103 17 3.5375872565757334 1103 18 2.138161978598877 1103 19 4.359693019573445 1103 20 4.83901699729434 1103 21 6.979336374667994 1103 22 2.521766524058634 1103 23 4.7525651726436 1103 24 6.231020319679168 1103 25 1.7656515297538036 1104 1 5.9150071798210995 1104 2 10.778627764265906 1104 3 7.085108416638654 1104 4 7.8090119715368616 1104 5 7.596365620197467 1104 6 7.111819406629255 1104 7 7.247108748475888 1104 8 7.366214502136039 1104 9 6.000805944996106 1104 10 5.113760406201795 1104 11 8.143691779322719 1104 12 5.180476739767318 1104 13 9.651294241697638 1104 14 7.255805541245067 1104 15 6.622830039610086 1104 16 6.080618066596987 1104 17 6.4253236537520415 1104 18 4.110991917883656 1104 19 4.798724335844543 1104 20 4.253641666846679 1104 21 3.4484485961478164 1104 22 5.478358773426791 1104 23 5.8763900862188265 1104 24 6.812136869660286 1104 25 6.033993190852488 1105 1 3.819931664579755 1105 2 9.836769467864979 1105 3 6.422245840372096 1105 4 5.9062256600687775 1105 5 1.8986384322472496 1105 6 4.654335483397983 1105 7 5.591777637772989 1105 8 3.3343136322978637 1105 9 3.9696926379993265 1105 10 3.4491812385409943 1105 11 6.770451742977993 1105 12 4.256821068716622 1105 13 5.500182820910343 1105 14 3.870265026098732 1105 15 4.613821981695011 1105 16 4.532006999473975 1105 17 5.255446560006725 1105 18 2.8047270982946664 1105 19 2.5778252755006186 1105 20 1.585664506872888 1105 21 2.704821063401319 1105 22 4.217443215787959 1105 23 1.853195340016054 1105 24 4.6583249317841435 1105 25 5.227463386983086 1106 1 6.575501931118474 1106 2 11.36743039589858 1106 3 7.873252867903229 1106 4 9.325942831295176 1106 5 8.664204744365726 1106 6 7.7495398378489995 1106 7 7.655347979891072 1106 8 7.23540251497845 1106 9 6.360388008628373 1106 10 5.734070224603354 1106 11 7.849411932898139 1106 12 5.380120701751821 1106 13 4.859703053910721 1106 14 5.996266621363144 1106 15 6.1234023407779645 1106 16 6.046122120340218 1106 17 6.6138142077205195 1106 18 6.551416114858693 1106 19 6.3666383151001575 1106 20 5.902753000859608 1106 21 5.937848435277427 1106 22 6.375684133105484 1106 23 6.74085134054511 1106 24 7.562237243527424 1106 25 6.693991176570137 1107 1 3.9813404668453707 1107 2 -14.26181031492533 1107 3 -5.883993627781093 1107 4 1.5718105537951068 1107 5 4.491418785513304 1107 6 4.828897938818962 1107 7 4.6146977867780485 1107 8 5.119410683075362 1107 9 3.2709378984561788 1107 10 2.5457623881385203 1107 11 5.601059266389829 1107 12 2.872182923122473 1107 13 4.614841251443757 1107 14 3.7851598993500986 1107 15 3.5110155477927663 1107 16 3.231857085018188 1107 17 3.826535126263959 1107 18 -1.2963480917112165 1107 19 1.541184798054637 1107 20 1.9079860160343642 1107 21 -1.218113133721598 1107 22 1.8687811463169623 1107 23 -1.1845051934730968 1107 24 2.5369973778600716 1107 25 3.3320994694720056 1108 1 5.3354404527576555 1108 2 10.706585146987148 1108 3 0.15391022553814437 1108 4 6.04030410306985 1108 5 7.548113280903195 1108 6 7.305774995197471 1108 7 7.548707374323982 1108 8 7.239900803900561 1108 9 5.4559596290859 1108 10 4.63656758663706 1108 11 7.016940466136511 1108 12 2.806251099763987 1108 13 3.7869200583854443 1108 14 5.054093722637486 1108 15 5.13516518302627 1108 16 4.870220171518074 1108 17 0.3184261321136539 1108 18 3.597446335609998 1108 19 4.756134365351123 1108 20 5.196775617983999 1108 21 5.39328316950396 1108 22 2.0780469130375643 1108 23 4.83514914402906 1108 24 5.787719866988173 1108 25 6.422521534783705 1109 1 3.494994321221359 1109 2 -14.26181031492533 1109 3 -5.892875295801673 1109 4 1.5946132958248356 1109 5 0.7091229769423215 1109 6 2.3974850239159444 1109 7 3.5899397096192693 1109 8 3.750311961454088 1109 9 3.11601678169189 1109 10 2.2469673705376536 1109 11 0.82738574464884 1109 12 1.1969630904375919 1109 13 2.82904664714259 1109 14 3.2715181589308138 1109 15 3.379653226473625 1109 16 2.9925750863578493 1109 17 3.5059478131395565 1109 18 3.5502158200991794 1109 19 2.1711419003961936 1109 20 1.4546703548261029 1109 21 0.4371101193745872 1109 22 2.6353630152867034 1109 23 2.93594387802775 1109 24 4.1280408883422055 1109 25 4.625709001018173 1110 1 3.554814325778203 1110 2 9.732994879879865 1110 3 6.752146349219974 1110 4 8.01475455807136 1110 5 8.762403906539042 1110 6 4.231080375932347 1110 7 6.881896875260647 1110 8 7.519974754801094 1110 9 5.997322318260583 1110 10 5.451079924650205 1110 11 3.5456473076472435 1110 12 6.138373434479515 1110 13 10.60351691371812 1110 14 8.054224925848104 1110 15 7.208201870811742 1110 16 6.893258613357165 1110 17 7.20791864932092 1110 18 7.299144961133582 1110 19 6.125937981508063 1110 20 6.368637089845468 1110 21 4.142293532452737 1110 22 6.210835788127898 1110 23 6.7761694419375225 1110 24 8.070977776015933 1110 25 8.463371338680513 1111 1 5.51018345923401 1111 2 9.676865073463876 1111 3 7.41249401463714 1111 4 8.3199794503772 1111 5 8.754790805548996 1111 6 7.722130345314287 1111 7 7.606918378695887 1111 8 7.768776048900833 1111 9 6.008595427816605 1111 10 5.162162246525664 1111 11 7.400301783724451 1111 12 6.452417036012893 1111 13 8.086969356614553 1111 14 6.313392468580652 1111 15 5.691826341190101 1111 16 5.6630589411296945 1111 17 6.804199882396919 1111 18 6.496636757971249 1111 19 5.600606403846147 1111 20 5.007486983478682 1111 21 7.847107824184441 1111 22 7.06287445537507 1111 23 7.003004313253182 1111 24 7.091608331070038 1111 25 7.695237482893344 1112 1 -2.215531994568117 1112 2 7.78818968507467 1112 3 6.043847868040135 1112 4 -0.32993324000626956 1112 5 4.045370375098731 1112 6 3.312010744998311 1112 7 5.9056697125928075 1112 8 5.9070024164050245 1112 9 4.77145835171865 1112 10 3.3399052236449975 1112 11 -0.1178863338524998 1112 12 3.912113253982046 1112 13 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3.7357395585576327 2204 7 5.026008695643654 2204 8 4.837510890981296 2204 9 3.314678690701701 2204 10 2.7263459016111007 2204 11 4.593130615300044 2204 12 4.551096304829503 2204 13 2.485291071763463 2204 14 3.88626689517821 2204 15 3.943718049548263 2204 16 3.905941128582054 2204 17 -1.4521848065452474 2204 18 2.120584538505362 2204 19 2.9723610499650306 2204 20 3.4366061264585746 2204 21 4.974368519612469 2204 22 4.350156354567108 2204 23 3.366080660662302 2204 24 4.945763160123352 2204 25 4.951697701583143 2205 1 5.662660376907837 2205 2 10.176000630348302 2205 3 6.949247423932988 2205 4 7.209172080847093 2205 5 7.441936977116889 2205 6 6.975206067819233 2205 7 7.141454067653658 2205 8 6.8592890060556435 2205 9 5.603740444081698 2205 10 5.023003854783095 2205 11 7.185101551579098 2205 12 6.310269366076938 2205 13 9.43224556483483 2205 14 7.084051981048297 2205 15 5.768611941669576 2205 16 5.5440458029503255 2205 17 6.199990756318396 2205 18 6.261540774275662 2205 19 4.377504822627142 2205 20 4.904901654468244 2205 21 -1.4592894616675736 2205 22 3.4160865903936255 2205 23 4.7042907402080765 2205 24 6.583766645521223 2205 25 6.737980974484177 2206 1 4.4222969528003055 2206 2 9.318781790337827 2206 3 6.375984353321133 2206 4 1.8953260151230822 2206 5 6.1083554330826715 2206 6 6.433066164266819 2206 7 6.604961639443832 2206 8 6.392910645664614 2206 9 4.996608886955048 2206 10 4.05376561615169 2206 11 3.6323199551751557 2206 12 5.0973468623674165 2206 13 8.597796647142587 2206 14 0.7240400887553733 2206 15 2.8432497177016955 2206 16 3.7378601740771487 2206 17 4.741035532437802 2206 18 -0.5880517237604721 2206 19 1.3370410232032093 2206 20 2.9648677232471563 2206 21 3.807943452707921 2206 22 -1.7316874233097863 2206 23 -2.8648236658318993 2206 24 1.5214071164123801 2206 25 3.0953361940006303 2207 1 1.8989308153492388 2207 2 -14.26181031492533 2207 3 -6.2818560120551155 2207 4 -4.032195461605934 2207 5 1.4924708093332164 2207 6 2.2576053660937028 2207 7 3.311412813867495 2207 8 3.3972671593662227 2207 9 1.0649728495474804 2207 10 0.7107414752208498 2207 11 -1.381900901719149 2207 12 -2.0587930354142294 2207 13 0.6946621321360631 2207 14 -5.505790815985819 2207 15 -1.4636989556345377 2207 16 -0.2914324531520833 2207 17 0.9538454497931745 2207 18 0.8486136742386599 2207 19 0.42652830028020006 2207 20 0.22239545269474092 2207 21 -3.53980013145911 2207 22 -3.954886368503349 2207 23 -0.9140706210298113 2207 24 0.9570462529935035 2207 25 1.5841112048749224 2208 1 4.620305928327241 2208 2 6.348732564697013 2208 3 6.085304615029692 2208 4 7.946277270868659 2208 5 3.600372442068551 2208 6 5.935022119999978 2208 7 6.370457881524249 2208 8 6.3767913187773715 2208 9 4.625953010935337 2208 10 4.20092750691321 2208 11 6.152303960724037 2208 12 5.962559319404974 2208 13 8.379543562128717 2208 14 2.7126641001015983 2208 15 4.343700046907873 2208 16 4.623832419304694 2208 17 5.550830969101849 2208 18 0.5776862467559063 2208 19 2.907539542810646 2208 20 3.9225820695969027 2208 21 6.477538984614004 2208 22 5.439292094475629 2208 23 1.1069026478870967 2208 24 2.7608793771685214 2208 25 4.2545314505329905 2209 1 3.0287734411523317 2209 2 4.990993826575963 2209 3 5.0283786587568535 2209 4 0.4959650358305865 2209 5 4.8623729050412585 2209 6 5.360225892480798 2209 7 5.857469189055568 2209 8 4.480553549029583 2209 9 4.323048705764364 2209 10 3.4859808879377105 2209 11 -0.32623806898360286 2209 12 2.971093087561549 2209 13 1.9605824538725294 2209 14 3.364328052517237 2209 15 3.8830469567008343 2209 16 3.5081632370624796 2209 17 1.103632598874384 2209 18 2.917961002727882 2209 19 3.8466667781643835 2209 20 3.9392260062497435 2209 21 6.213195825443037 2209 22 4.927784643126799 2209 23 0.6005829346857894 2209 24 -2.804156408177782 2209 25 0.39341104300839547 2210 1 -2.1018458631812535 2210 2 -14.26181031492533 2210 3 -7.822680648599727 2210 4 -2.4468452776160667 2210 5 -0.5763233029910886 2210 6 -1.5396996348111678 2210 7 -1.308251480033693 2210 8 -21.65231961749328 2210 9 -13.817799968212068 2210 10 -7.612460853306629 2210 11 -10.740987286469103 2210 12 -5.0528224134681565 2210 13 -5.778896111213154 2210 14 -4.3908393772453955 2210 15 -5.864011135160386 2210 16 -5.545790901604757 2210 17 -5.173198044204533 2210 18 -5.322264651151944 2210 19 -10.639608515790258 2210 20 -7.638990805373666 2210 21 -5.497161234191811 2210 22 -10.20378092119684 2210 23 -8.234145121844193 2210 24 -10.728166692654082 2210 25 -8.364193675380854 2211 1 0.09840389145147554 2211 2 5.0222502371672775 2211 3 0.7448213500873138 2211 4 1.6382776660112093 2211 5 1.0679136535447817 2211 6 0.16479611107283887 2211 7 -21.19602520266143 2211 8 -13.487582306718569 2211 9 -9.116778362892635 2211 10 -7.1229198363486645 2211 11 -9.946675744782667 2211 12 -4.940857752854967 2211 13 -6.837009968126662 2211 14 -4.653055742429892 2211 15 -5.132104127731122 2211 16 -11.761590603914135 2211 17 -7.634521012531032 2211 18 -5.615959524763422 2211 19 -8.412439046628538 2211 20 -6.539199448801398 2211 21 -4.0895907601617445 2211 22 -5.873358142014382 2211 23 -8.398329137547698 2211 24 -22.999152094113935 2211 25 -22.80762433231516 2212 1 -0.8706482736378831 2212 2 -14.26181031492533 2212 3 -7.9558210322357805 2212 4 -20.05450951119271 2212 5 -20.521578777443644 2212 6 -21.349006204154232 2212 7 -9.987653109638176 2212 8 -9.801156826795605 2212 9 -5.270898762983752 2212 10 -5.586133404656145 2212 11 -6.144303361838311 2212 12 -2.8815722012800755 2212 13 -6.476277710262552 2212 14 -4.070603424096522 2212 15 -4.008457748638573 2212 16 -4.15556852849323 2212 17 -4.415010979190104 2212 18 -3.409837219476504 2212 19 -4.0131257454626414 2212 20 -4.155317405271816 2212 21 -1.8805112596543836 2212 22 -3.1741637084995062 2212 23 -3.336300104020392 2212 24 -2.0949660476023055 2212 25 -2.4876243323151606 2213 1 -19.365531994568116 2213 2 -14.26181031492533 2213 3 -8.31301628271003 2213 4 -5.253905281585457 2213 5 -20.521578777443644 2213 6 -10.3751894871351 2213 7 -21.19602520266143 2213 8 -10.485149426153905 2213 9 -7.817817426184295 2213 10 -6.4695985911944724 2213 11 -5.892052957847586 2213 12 -4.426021485806643 2213 13 -19.93674282654162 2213 14 -12.370836216433581 2213 15 -6.414044764727944 2213 16 -5.64129887346618 2213 17 -4.224976552154342 2213 18 -4.121213129919372 2213 19 -4.945209400817568 2213 20 -4.990032035591028 2213 21 -2.9197590172131065 2213 22 -3.990796416525452 2213 23 -3.856315092661813 2213 24 -2.7292628695419303 2213 25 -3.6011792165044874 2214 1 -19.365531994568116 2214 2 -14.26181031492533 2214 3 -11.7739971943993 2214 4 -20.05450951119271 2214 5 -20.521578777443644 2214 6 -21.349006204154232 2214 7 -16.0644178671706 2214 8 -14.610248420082277 2214 9 -12.893144065409682 2214 10 -12.872180418027611 2214 11 -13.709623850536536 2214 12 -11.601603309380724 2214 13 -19.93674282654162 2214 14 -16.544850432450552 2214 15 -24.75543449282462 2214 16 -18.13958297870773 2214 17 -24.508964467562198 2214 18 -17.686663189723113 2214 19 -14.187445506632706 2214 20 -12.695888891185366 2214 21 -10.019538378893738 2214 22 -11.194549549296273 2214 23 -11.046083601674741 2214 24 -9.843812288288692 2214 25 -9.622079564246121 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 108578.0 3 1.0 4 470.0 5 1680.0 6 2492.0 7 2444.0 8 2128.0 9 2061.0 10 1892.0 11 1784.0 12 1794.0 13 1969.0 14 3060.0 15 6867.0 16 10591.0 17 11137.0 18 11312.0 19 13187.0 20 15313.0 21 17769.0 22 18625.0 23 20332.0 24 21099.0 25 21986.0 26 18110.0 27 14170.0 28 15332.0 29 17415.0 30 17088.0 31 19856.0 32 26064.0 33 32956.0 34 40394.0 35 47068.0 36 46951.0 37 49139.0 38 36659.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08928204492462 19.305327475617855 12.793006064331843 25.812384415125678 2 13.047456881074007 20.833767708007553 42.997996084836274 23.12077932608217 3 18.48918186167668 25.684909017564816 29.44991509973826 26.375994021020244 4 11.014604491537854 18.121884903916026 39.308461462359574 31.555049142186547 5 11.739320580822907 38.47858160228299 36.50121075676127 13.280887060132834 6 29.648534016657763 39.38314062961983 18.851074351299992 12.117251002422414 7 26.40783468045951 31.2964028893627 23.35324972233257 18.942512707845218 8 23.233320802563924 39.165132178378684 21.518729252550223 16.082817766507166 9 26.394348233970387 15.274318897098565 20.782198763197542 37.5491341057335 10 14.172536789087845 29.52611831059968 35.89871126167303 20.402633638639443 11 32.50264671892861 22.090446634796574 26.439822916129113 18.9670837301457 12 23.666447344772454 24.623701455495105 33.154593803869965 18.555257395862473 13 28.260980327736362 22.297808941682465 25.702198086008394 23.739012644572778 14 19.40941460488784 23.40174435388456 28.401766790266198 28.787074250961396 15 21.368901422117105 33.6782497953243 23.644895236362533 21.307953546196064 16 20.435263180269526 28.21573676507206 30.261631614427394 21.08736844023102 17 20.30345977490621 29.266436772897798 29.872143696237067 20.557959755958922 18 20.16639566719671 27.509385349290422 32.635310433878125 19.68890854963475 19 22.717305250578836 26.792889685562777 27.704234819628056 22.78557024423034 20 22.86492882604282 28.179811619178018 29.19386345931399 19.76139609546517 21 21.371996675778977 26.630618295858753 27.04660847738034 24.950776550981928 22 22.16991082127902 29.66520204717601 28.11261562490182 20.052271506643148 23 20.79236646617693 28.906457104818234 29.326631360050897 20.97454506895394 24 22.302640744112512 27.502648519582845 29.84403702419791 20.350673712106733 25 21.67885415516035 28.073787694686843 29.53422438234103 20.713133767811776 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 648.0 1 648.0 2 1484.5 3 2321.0 4 2321.0 5 2321.0 6 3339.0 7 4357.0 8 4357.0 9 4357.0 10 5095.0 11 5833.0 12 5833.0 13 5833.0 14 7427.5 15 9022.0 16 9022.0 17 9022.0 18 12861.5 19 16701.0 20 16701.0 21 16701.0 22 23693.5 23 30686.0 24 30686.0 25 30686.0 26 40186.5 27 49687.0 28 49687.0 29 49687.0 30 60197.0 31 70707.0 32 70707.0 33 70707.0 34 76296.0 35 81885.0 36 81885.0 37 81885.0 38 83433.5 39 84982.0 40 84982.0 41 84982.0 42 84222.0 43 83462.0 44 83462.0 45 83462.0 46 80737.0 47 78012.0 48 78012.0 49 78012.0 50 70156.5 51 62301.0 52 62301.0 53 62301.0 54 52512.5 55 42724.0 56 42724.0 57 42724.0 58 34926.5 59 27129.0 60 27129.0 61 27129.0 62 21468.0 63 15807.0 64 15807.0 65 15807.0 66 11897.0 67 7987.0 68 7987.0 69 7987.0 70 5717.0 71 3447.0 72 3447.0 73 3447.0 74 2395.0 75 1343.0 76 1343.0 77 1343.0 78 927.0 79 511.0 80 511.0 81 511.0 82 339.5 83 168.0 84 168.0 85 168.0 86 105.0 87 42.0 88 42.0 89 42.0 90 26.5 91 11.0 92 11.0 93 11.0 94 5.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.375053142740297 2 36.50012577728153 3 5.913591743126014 4 13.756798225289913 5 13.552171092408791 6 14.920274856459436 7 9.271918714041306 8 7.6474058251798755 9 0.11239045975641869 10 1.5124166449682468 11 5.928743860082704 12 5.872842846067732 13 16.269548805262936 14 1.6499625610314033 15 2.9705504631693227 16 3.1094203506170444 17 2.949219813084662 18 1.5659639320773258 19 1.5185951780962175 20 0.2937745394418431 21 4.589767466492491 22 1.6682039445520782 23 0.11312599941451043 24 9.186449005771044 25 0.11739212943144256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 679773.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.96211248314498 #Duplication Level Percentage of deduplicated Percentage of total 1 96.71795633253765 87.97669623060195 2 2.285796975902814 4.158418432714089 3 0.43265252660289993 1.180649633729096 4 0.18396050715779946 0.669337453781766 5 0.09934410484686519 0.451827481980895 6 0.0600007523247749 0.32746771092236987 7 0.03886605337424411 0.24747368231627362 8 0.030430149608157982 0.22143925532289574 9 0.018778812014660046 0.15373443696996075 >10 0.11282923552535429 1.9437715425373352 >50 0.009859530023584763 0.6092422242137869 >100 0.008689492005512289 1.3862655159283623 >500 6.684224605369577E-4 0.49948119292693527 >1k 1.6710561513423944E-4 0.1741952060542507 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1146 0.16858568963462803 No Hit GTCCTAAAGTGTGTATTTCTCATTT 961 0.14137072228523345 No Hit CTGTAGGACGTGGAATATGGCAAGA 874 0.1285723322344371 No Hit CTTTAGGACGTGAAATATGGCGAGG 737 0.10841854560272325 No Hit GTCCTACAGTGTGCATTTCTCATTT 714 0.10503506317550122 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTTAG 40 2.0282776E-5 23.34491 1 TAGGACC 305 0.0 16.502678 4 CCAATAC 45 0.0045168614 14.5856905 3 TATACTT 50 7.4653595E-4 14.582884 5 CAAGGGC 45 0.0052016596 14.278911 4 TGTAGGA 710 0.0 14.247317 2 GTCCTAA 375 0.0 14.229279 1 GTTCTAA 75 1.5235749E-4 14.229278 1 GCAGAAC 55 0.0010598012 13.922706 3 GTCCTAC 730 0.0 13.705426 1 ATACACC 55 0.0012444033 13.62987 4 AGGACCT 560 0.0 13.020432 5 CTGTAGG 750 0.0 12.984217 1 CTAGAGT 100 4.5140587E-7 12.85102 4 TTAAAAC 60 0.0020417813 12.762479 3 TCCTAAA 480 0.0 12.53058 2 GTCTTAT 75 0.0024572657 12.450618 1 GTCTTAG 75 0.0024572657 12.450618 1 GTAGGAC 770 0.0 12.359952 3 TCCTACA 855 0.0 12.310745 2 >>END_MODULE