FastQCFastQC Report
Thu 26 May 2016
SRR1926686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679773
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA23310.34290858860237167No Hit
GTCCTACAGTGGACATTTCTAAATT21800.320695290928001No Hit
CTTTAGGACGTGAAATATGGCGAGG21190.3117217070992817No Hit
GTCCTAAAGTGTGTATTTCTCATTT20520.3018654756808523No Hit
GTCCTACAGTGTGCATTTCTCATTT14170.20845193910320062No Hit
CTGTAGGACCTGGAATATGGCGAGA12280.18064854002733263No Hit
GTATCAACGCAGAGTACTTTTTTTT11980.17623530207878219No Hit
CTGAAGGACCTGGAATATGGCGAGA11210.1649079913441693No Hit
GGTATCAACGCAGAGTACTTTTTTT7670.11283178355127374No Hit
GTCCTTCAGTGTGCATTTCTCATTT7030.1034168759276994No Hit
GTACATGGGAAGCAGTGGTATCAAC7030.1034168759276994No Hit
GAATATGGCAAGAAAACTGAAAATC6980.10268133626960764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG402.683937E-416.6846031
CCAACGT456.910244E-414.73366719
TAGGACC3950.014.7300264
CTACACC602.4685638E-514.3074454
GATATAC2500.013.7290451
GATTTAT500.001458155613.3476821
TCCAACG1102.8558134E-912.91682918
TAAGACC603.950322E-412.7177294
GGCGAGG5150.012.69016419
AGGACCT7950.012.3577935
ATAGGAC1103.5810444E-812.1423483
GACGTGG5600.012.0503517
GGACCTG8000.011.922876
GTAGGAC11400.011.7999893
TTATAGT657.747157E-411.7394424
AGGACGT11550.011.7267375
CGGTTTC658.161088E-411.66684813
GCCGGTT658.161088E-411.66684811
TTAGGAC7650.011.5981523
GGACGTG11500.011.5288976
Produced by FastQC (version 0.11.5)