##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926686_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679773 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.65623818539424 31.0 28.0 31.0 23.0 34.0 2 29.11865284440541 31.0 29.0 33.0 25.0 34.0 3 29.44317294155549 31.0 30.0 33.0 25.0 34.0 4 33.23481662260784 35.0 33.0 37.0 28.0 37.0 5 32.93436926738779 35.0 33.0 37.0 26.0 37.0 6 33.00047810077776 35.0 33.0 37.0 27.0 37.0 7 32.83991420665428 35.0 33.0 37.0 26.0 37.0 8 32.81016162748447 35.0 33.0 37.0 26.0 37.0 9 34.089828516284115 38.0 34.0 39.0 25.0 39.0 10 33.98586145669216 37.0 34.0 39.0 25.0 39.0 11 34.23294540971766 38.0 34.0 39.0 27.0 39.0 12 34.059063834544766 38.0 34.0 39.0 25.0 39.0 13 34.20570396294057 38.0 34.0 39.0 27.0 39.0 14 34.8590867833821 39.0 34.0 40.0 25.0 41.0 15 34.998549515794245 39.0 34.0 40.0 25.0 41.0 16 34.99264754557772 39.0 34.0 40.0 25.0 41.0 17 34.919072984658115 39.0 34.0 40.0 25.0 41.0 18 34.899450257659545 39.0 34.0 41.0 25.0 41.0 19 34.933327743231935 39.0 34.0 41.0 25.0 41.0 20 34.93431042421514 39.0 34.0 41.0 25.0 41.0 21 34.84916729555307 39.0 34.0 41.0 24.0 41.0 22 34.79686748370412 39.0 34.0 41.0 24.0 41.0 23 34.46319580212806 39.0 34.0 40.0 19.0 41.0 24 34.556347486587434 39.0 34.0 41.0 22.0 41.0 25 34.34903269179564 39.0 34.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.523971174636916 1101 2 0.5271249738556705 1101 3 0.4297181423576468 1101 4 0.1933466946719804 1101 5 0.475401476599302 1101 6 0.15663140481466087 1101 7 0.30145592094332585 1101 8 0.4070720046100078 1101 9 0.7983846701279376 1101 10 0.7443033925429177 1101 11 0.4482620978287528 1101 12 0.5492705353928571 1101 13 0.4046237774347361 1101 14 0.5615435353150531 1101 15 0.7502161873158499 1101 16 0.631531853746182 1101 17 0.40449928376344957 1101 18 0.6734109917448095 1101 19 0.6901788989551747 1101 20 0.7020164297995279 1101 21 0.6375949744299731 1101 22 0.49124970200436024 1101 23 0.7720185249064642 1101 24 0.6349199888109425 1101 25 0.6625937892594465 1102 1 0.613837191654028 1102 2 0.9047735840862039 1102 3 0.8436815546969356 1102 4 0.5279639833404559 1102 5 0.7614759894345156 1102 6 0.35999936296379786 1102 7 0.7032709734740337 1102 8 0.8253164743379031 1102 9 1.217069911444959 1102 10 1.1329669499242883 1102 11 0.6490578027303613 1102 12 0.9508908957109625 1102 13 0.6884198847484981 1102 14 1.0355792660405214 1102 15 0.8598431020305242 1102 16 0.958343359838814 1102 17 0.9178744216402777 1102 18 0.9802068356088967 1102 19 1.066552239005027 1102 20 0.876499735485119 1102 21 1.0453029042655047 1102 22 1.2351852928988478 1102 23 1.322692058635262 1102 24 1.1655510810849137 1102 25 1.1824067978838073 1103 1 0.07417016949500521 1103 2 0.07535362911523436 1103 3 -0.06240921435152913 1103 4 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0.5312426243051362 2202 16 0.4206425686616413 2202 17 0.659818355504612 2202 18 0.6938157934038998 2202 19 0.4264468375114632 2202 20 0.43053243037132205 2202 21 0.6520829641143706 2202 22 0.46677555014242245 2202 23 0.8188516082592088 2202 24 0.7288052967006777 2202 25 0.6357286764634154 2203 1 0.049533105855598336 2203 2 0.22340959892379786 2203 3 0.07955444636003861 2203 4 -0.2547631983958354 2203 5 0.1427524716017956 2203 6 -0.18252603110298793 2203 7 0.365329705120395 2203 8 0.20584303915532587 2203 9 0.45688574569577867 2203 10 0.7373897182946081 2203 11 0.3349121928936327 2203 12 0.3159634003590881 2203 13 0.13071795300993472 2203 14 0.5717891276432354 2203 15 0.49071694909894603 2203 16 0.4536686980977933 2203 17 0.5328506274982772 2203 18 0.41871816246973736 2203 19 0.6952567093920123 2203 20 0.6461939754526043 2203 21 0.6989131231594783 2203 22 0.6294674195400987 2203 23 0.8655806583750305 2203 24 0.6165903497600596 2203 25 0.7388962747766499 2204 1 -0.4132348568054063 2204 2 -0.23377845738388814 2204 3 -0.37135135345146963 2204 4 -0.6525095445295435 2204 5 -0.4536355488030068 2204 6 -0.7907141322892741 2204 7 -0.3393698326299912 2204 8 -0.17159947883351379 2204 9 0.020914049134979962 2204 10 0.08132736695498011 2204 11 -0.16149901478958384 2204 12 -0.1938612738970491 2204 13 -0.3365932202663515 2204 14 -0.13703998959463348 2204 15 -0.21244998434607965 2204 16 -0.050602996967057834 2204 17 -0.2988912606941625 2204 18 -0.39888920958884455 2204 19 -0.10305286321408857 2204 20 -0.07596109508160254 2204 21 -0.06502093521262253 2204 22 -0.14815386488552917 2204 23 0.1151610434334458 2204 24 -0.12032753735529411 2204 25 -0.20739750056106487 2205 1 0.28850081511746595 2205 2 0.5410381376072557 2205 3 0.3081803664799452 2205 4 0.12962919679210216 2205 5 -0.014173255748502811 2205 6 -0.12535308442127047 2205 7 -0.1887936176596412 2205 8 0.027992954480147603 2205 9 0.2642566581239336 2205 10 0.3183288004653946 2205 11 0.3328681325246876 2205 12 0.24152535229402616 2205 13 0.1866760267190628 2205 14 0.5430932425497303 2205 15 0.4287254941934222 2205 16 0.382712066135511 2205 17 0.2880595783310511 2205 18 0.36246222532697203 2205 19 0.45069253409315735 2205 20 0.43765444304696643 2205 21 0.43286252882312937 2205 22 0.15287348424452318 2205 23 0.5087805166886383 2205 24 0.2657716278356972 2205 25 0.5130089418033847 2206 1 0.5136551967182363 2206 2 0.4538302625090651 2206 3 0.6354979998045494 2206 4 0.46146235555966797 2206 5 0.7382829391541748 2206 6 0.7432343512076045 2206 7 0.7410773127759 2206 8 0.7435544016303623 2206 9 0.8648842766104465 2206 10 0.8631990217275032 2206 11 0.7594727737920408 2206 12 0.6405499660672689 2206 13 0.627420495166298 2206 14 0.832869689263525 2206 15 0.856352981755613 2206 16 0.916970598037409 2206 17 0.6550895600016418 2206 18 0.7990116601703008 2206 19 0.6963338450820729 2206 20 0.7756055864527411 2206 21 0.9582618480166332 2206 22 0.9260934589324918 2206 23 1.0814244243433464 2206 24 1.0688450763824378 2206 25 0.9195371559955845 2207 1 -0.8442966301630186 2207 2 0.07870928599753668 2207 3 0.2835582977601838 2207 4 0.32516940210163625 2207 5 0.12582235846691958 2207 6 0.1899204103637544 2207 7 0.21243714313692408 2207 8 0.2526616221610283 2207 9 0.29396349583619497 2207 10 0.09768820180580207 2207 11 0.09476937013827325 2207 12 0.2505216929050249 2207 13 -0.05516024646049544 2207 14 -5.238272655234081 2207 15 -2.1858216050212462 2207 16 -0.9010372314536781 2207 17 -0.6298549811031862 2207 18 -0.2729612084682387 2207 19 -0.1727124794068331 2207 20 0.14821596052842523 2207 21 -0.20337364567627958 2207 22 0.01939761291247777 2207 23 0.3249243156004198 2207 24 -0.04991851648402701 2207 25 -0.05233376614447849 2208 1 0.4286426185576495 2208 2 0.6965919969494152 2208 3 0.7815230681303618 2208 4 0.5570951974992866 2208 5 0.6084968972890863 2208 6 0.5684432107877058 2208 7 0.752354953672004 2208 8 0.9153239366007497 2208 9 1.0784664901596628 2208 10 0.9817074226964877 2208 11 0.726953958313139 2208 12 0.5742480488036108 2208 13 0.6199130673224929 2208 14 1.0870688797061945 2208 15 0.7001398621647397 2208 16 0.9171569411572023 2208 17 0.5351130599395262 2208 18 0.8321606777279626 2208 19 0.5014784680478286 2208 20 0.6690988470432444 2208 21 0.7698068395276678 2208 22 0.8901124141687333 2208 23 1.3031766190512073 2208 24 1.0450733881137708 2208 25 1.2071072934753957 2209 1 -0.34627254271230967 2209 2 -0.42582083757721634 2209 3 -0.07248032202461374 2209 4 0.22143862820850302 2209 5 0.2820841686623723 2209 6 0.1010050587140725 2209 7 0.18523714884147324 2209 8 0.5310328313111228 2209 9 0.5027320980082024 2209 10 0.14702397858686567 2209 11 0.2695973423856515 2209 12 0.1872497072234367 2209 13 0.22900321734058338 2209 14 0.2799954442247028 2209 15 0.399237472696079 2209 16 0.2771949293575062 2209 17 0.21208912859525952 2209 18 0.19519101737478195 2209 19 0.11981313757560486 2209 20 0.014308131750411235 2209 21 0.10666564439281956 2209 22 0.20222050811281633 2209 23 0.2652305071733778 2209 24 0.1321000375558654 2209 25 0.2766068281267309 2210 1 -0.10900375449190847 2210 2 -0.5415416641140638 2210 3 0.5543476444453503 2210 4 0.6464172153368466 2210 5 0.8308504093808367 2210 6 0.8242586500935047 2210 7 0.9134385306014821 2210 8 0.9081481396249842 2210 9 1.2286772558651506 2210 10 1.0854966110475175 2210 11 0.8335399558896199 2210 12 0.7877359053680735 2210 13 0.9673347767644529 2210 14 1.3614785060134338 2210 15 1.037352069346852 2210 16 1.2365057497853869 2210 17 1.300474692541023 2210 18 1.2618370059251944 2210 19 1.2256029576444973 2210 20 1.247476984839217 2210 21 1.2095584288848613 2210 22 1.2399260559359462 2210 23 1.444286986771317 2210 24 1.4793569857178213 2210 25 1.493190152538034 2211 1 -1.0557005585425792 2211 2 -0.17920082785040492 2211 3 0.30101081205556923 2211 4 0.5605947312450965 2211 5 0.7853014691724525 2211 6 0.32079182179859345 2211 7 0.7534135713877035 2211 8 0.6000155958001159 2211 9 0.8776899369657016 2211 10 1.0513954352345607 2211 11 0.9006015934480374 2211 12 1.0608703016345942 2211 13 0.815041324008213 2211 14 1.1665520836957342 2211 15 0.852805007579299 2211 16 0.9007312016836551 2211 17 0.9995821081574263 2211 18 1.0186455414563298 2211 19 0.9425815603185157 2211 20 1.1503229201922522 2211 21 1.2614909805094854 2211 22 1.3512220501121277 2211 23 1.3591096073587678 2211 24 1.3494228286437675 2211 25 1.4556554598808518 2212 1 1.1421573387011001 2212 2 0.2016768047367279 2212 3 0.6844231834030836 2212 4 0.4326343261839014 2212 5 0.6072907420917488 2212 6 0.6609255996776113 2212 7 0.9323487326045381 2212 8 0.9937533000391738 2212 9 1.1735983218980834 2212 10 0.8838500315194224 2212 11 0.8563646036573971 2212 12 0.9871044336315293 2212 13 0.8323661806008147 2212 14 0.7687463722500638 2212 15 1.0021814701277094 2212 16 0.38808442912675645 2212 17 0.9877425587776543 2212 18 0.9598967580895135 2212 19 0.9290480433684891 2212 20 1.1920483649105122 2212 21 1.0316523008930076 2212 22 1.0420038016497486 2212 23 1.2757680595942702 2212 24 1.1846192501890442 2212 25 0.8705607178070878 2213 1 0.5576059440548526 2213 2 0.46828258004762446 2213 3 0.10245170866128461 2213 4 -0.748159603416326 2213 5 -1.549941043195961 2213 6 -0.2931370247398206 2213 7 -0.051130974180161104 2213 8 0.18487721394816248 2213 9 -0.30153973754124763 2213 10 -0.1550595448172558 2213 11 -0.31486750950684694 2213 12 -0.41573216675723756 2213 13 -0.467537798415087 2213 14 -8.049698087807549 2213 15 -3.2003722196542554 2213 16 -1.9252671716133065 2213 17 -0.9908630291752303 2213 18 -4.721203033474691 2213 19 -2.5057087425047158 2213 20 -4.809077656776175 2213 21 -1.6869387668134905 2213 22 -1.3046450660052358 2213 23 -3.955907623677003 2213 24 -1.7010408355272446 2213 25 -1.1625257713378545 2214 1 0.7653318701980503 2214 2 0.3551150024738625 2214 3 -1.1267807502664198 2214 4 -1.2205374457234797 2214 5 -0.09133598578763014 2214 6 -0.16693320968492031 2214 7 -0.3580202997830071 2214 8 -0.3498105844594619 2214 9 -4.960589982459556 2214 10 -1.1897457136951957 2214 11 -0.7281455691932592 2214 12 -0.7371894877452547 2214 13 -0.7894546467743169 2214 14 -2.2461965544201163 2214 15 -1.4539726654935805 2214 16 -0.9827341654895321 2214 17 -1.1691205404923082 2214 18 -0.5046906832692102 2214 19 -3.912765257853259 2214 20 -1.4305933149269237 2214 21 -2.873510138584173 2214 22 -5.420449222609555 2214 23 -5.81187349480728 2214 24 -5.290637266205422 2214 25 -3.0514342145829687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20577.0 3 17.0 4 397.0 5 2454.0 6 3079.0 7 2428.0 8 1781.0 9 1410.0 10 1271.0 11 1183.0 12 1085.0 13 1146.0 14 1096.0 15 1123.0 16 1214.0 17 1285.0 18 1481.0 19 1731.0 20 2203.0 21 2851.0 22 3664.0 23 4538.0 24 5455.0 25 6452.0 26 7561.0 27 8339.0 28 9760.0 29 11886.0 30 14569.0 31 18483.0 32 24143.0 33 31659.0 34 41760.0 35 56351.0 36 77556.0 37 116174.0 38 191611.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.33644749725762 20.050209451450698 9.856365630820651 29.756977420471024 2 12.714216342687287 21.66222758509057 41.47166590638198 24.151890165840165 3 17.789425820492934 25.986335295874834 29.645315137272444 26.578923746359784 4 11.619454050474578 18.367743359576192 38.82584062982856 31.18696196012067 5 12.383956962729382 39.629957165790906 35.74000473810214 12.246081133377574 6 30.59479625278213 38.97641225894971 18.254025689385 12.17476579888316 7 27.17029759026197 31.94701643056817 22.844003963152122 18.03868201601774 8 23.798542991482353 39.82902782057652 20.33471976692582 16.037709421015307 9 26.25341155897371 15.414864739335515 20.337683516438148 37.99404018525263 10 14.888204578926228 29.91470973797925 34.633702085837584 20.56338359725694 11 33.6308442356098 22.390257609036247 25.908457655818083 18.07044049953586 12 23.932756840095212 24.914747065798494 32.34168257956696 18.81081351453934 13 29.41790862537935 23.017683844459842 25.640912481078242 21.92349504908256 14 19.992138353205828 24.018214287291663 27.623382952004206 28.366264407498303 15 21.797674459659056 33.75549461865728 22.944409464572583 21.502421457111083 16 20.70757742484068 28.585698397660337 30.036954436591955 20.669769740907032 17 20.50448404105075 30.25756755484418 28.648873693625838 20.589074710479238 18 20.385877160720852 27.949981610886354 31.787716072085324 19.876425156307466 19 23.51639686714801 27.04291489399262 27.44261243608076 21.99807580277861 20 23.563255590894514 28.40962919592919 28.370203864270938 19.65691134890536 21 21.943304352942906 27.211998175853598 27.09887167718493 23.745825794018565 22 22.83703530443251 29.926754960847223 26.882797639800344 20.35341209491992 23 21.02557916477878 29.653324938361685 28.333029309827413 20.988066587032122 24 23.248485882704273 27.45947520407668 29.02824341798464 20.26379549523441 25 21.789185507515008 28.692666522500893 28.633676241921936 20.884471728062163 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 149.0 1 149.0 2 143.5 3 138.0 4 138.0 5 138.0 6 243.0 7 348.0 8 348.0 9 348.0 10 655.5 11 963.0 12 963.0 13 963.0 14 1687.0 15 2411.0 16 2411.0 17 2411.0 18 4816.5 19 7222.0 20 7222.0 21 7222.0 22 12274.5 23 17327.0 24 17327.0 25 17327.0 26 26451.0 27 35575.0 28 35575.0 29 35575.0 30 48223.5 31 60872.0 32 60872.0 33 60872.0 34 68571.0 35 76270.0 36 76270.0 37 76270.0 38 80639.5 39 85009.0 40 85009.0 41 85009.0 42 87651.0 43 90293.0 44 90293.0 45 90293.0 46 90696.0 47 91099.0 48 91099.0 49 91099.0 50 85045.5 51 78992.0 52 78992.0 53 78992.0 54 66735.0 55 54478.0 56 54478.0 57 54478.0 58 45618.0 59 36758.0 60 36758.0 61 36758.0 62 29329.0 63 21900.0 64 21900.0 65 21900.0 66 16740.0 67 11580.0 68 11580.0 69 11580.0 70 8400.5 71 5221.0 72 5221.0 73 5221.0 74 3625.5 75 2030.0 76 2030.0 77 2030.0 78 1404.0 79 778.0 80 778.0 81 778.0 82 526.5 83 275.0 84 275.0 85 275.0 86 172.0 87 69.0 88 69.0 89 69.0 90 42.5 91 16.0 92 16.0 93 16.0 94 8.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09091270174013971 2 0.029862910118524863 3 0.03118688150309 4 0.03280506875089184 5 0.025890995964829435 6 0.3948376884636489 7 0.22433959571798232 8 0.22566356710254748 9 0.23007680505109795 10 0.013828145572124811 11 0.0032363744956036794 12 0.004560345880168821 13 0.0 14 0.07737877203125161 15 0.0025008348375119343 16 0.0025008348375119343 17 0.004266130016932122 18 0.0033834824272220283 19 0.0013239713845651416 20 0.0010297555213284433 21 4.413237948550472E-4 22 0.0 23 1.471079316183491E-4 24 5.884317264733964E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 679773.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.45842784623648 #Duplication Level Percentage of deduplicated Percentage of total 1 92.4495209534712 74.38343111053989 2 5.5153679155619 8.875156629593697 3 0.9424457323740679 2.2748310587163734 4 0.34906601028304407 1.1234120960772775 5 0.18126746665307103 0.7292247693288096 6 0.10863586082057995 0.5244402341607852 7 0.08250001961292969 0.46464753127379976 8 0.05439113011526388 0.3500979854283375 9 0.043389870756153186 0.314197270694233 >10 0.22785537089569322 3.416003348768385 >50 0.021677795987121766 1.1898684885071436 >100 0.020391445809926096 3.3225656449263488 >500 0.002020773907913581 1.0151421701312577 >1k 0.0014696537512098773 2.0169816618536904 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2331 0.34290858860237167 No Hit GTCCTACAGTGGACATTTCTAAATT 2180 0.320695290928001 No Hit CTTTAGGACGTGAAATATGGCGAGG 2119 0.3117217070992817 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2052 0.3018654756808523 No Hit GTCCTACAGTGTGCATTTCTCATTT 1417 0.20845193910320062 No Hit CTGTAGGACCTGGAATATGGCGAGA 1228 0.18064854002733263 No Hit GTATCAACGCAGAGTACTTTTTTTT 1198 0.17623530207878219 No Hit CTGAAGGACCTGGAATATGGCGAGA 1121 0.1649079913441693 No Hit GGTATCAACGCAGAGTACTTTTTTT 767 0.11283178355127374 No Hit GTCCTTCAGTGTGCATTTCTCATTT 703 0.1034168759276994 No Hit GTACATGGGAAGCAGTGGTATCAAC 703 0.1034168759276994 No Hit GAATATGGCAAGAAAACTGAAAATC 698 0.10268133626960764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTGG 40 2.683937E-4 16.684603 1 CCAACGT 45 6.910244E-4 14.733667 19 TAGGACC 395 0.0 14.730026 4 CTACACC 60 2.4685638E-5 14.307445 4 GATATAC 250 0.0 13.729045 1 GATTTAT 50 0.0014581556 13.347682 1 TCCAACG 110 2.8558134E-9 12.916829 18 TAAGACC 60 3.950322E-4 12.717729 4 GGCGAGG 515 0.0 12.690164 19 AGGACCT 795 0.0 12.357793 5 ATAGGAC 110 3.5810444E-8 12.142348 3 GACGTGG 560 0.0 12.050351 7 GGACCTG 800 0.0 11.92287 6 GTAGGAC 1140 0.0 11.799989 3 TTATAGT 65 7.747157E-4 11.739442 4 AGGACGT 1155 0.0 11.726737 5 CGGTTTC 65 8.161088E-4 11.666848 13 GCCGGTT 65 8.161088E-4 11.666848 11 TTAGGAC 765 0.0 11.598152 3 GGACGTG 1150 0.0 11.528897 6 >>END_MODULE