FastQCFastQC Report
Thu 26 May 2016
SRR1926685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698145
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13090.18749686669674637No Hit
GTCCTAAAGTGTGTATTTCTCATTT11690.16744372587356493No Hit
CTGTAGGACGTGGAATATGGCAAGA9610.13765048807912397No Hit
CTTTAGGACGTGAAATATGGCGAGG9300.13321014975399093No Hit
GTCCTACAGTGTGCATTTCTCATTT8440.1208917918197509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGA250.003493379820.8778065
TGTAGGG500.002007622716.4720572
GTGTAGG801.5133932E-515.0647071
TCTAGAC450.004416217614.6353283
TGTAGGA8400.014.5437812
GTGTTAT658.1190816E-414.4209161
GTATAAA954.885418E-614.0956321
CTGTAGG8100.013.7214891
GTCCTAA4600.013.6819561
GACATGC450.007152979313.6089367
CTATAGT1354.7293724E-1113.5227844
TGTAGAA2350.013.4346562
GTGTTAG700.001421239713.3908511
GTAGAGC500.00887487413.1717963
GTAAGAC500.00887487413.1717963
GGACCTG7000.013.117986
TCCTAAA5450.012.8451822
TAGGACC4050.012.7273264
CTAGAGT1202.3088432E-812.5284624
AGGACCT7500.012.5266845
Produced by FastQC (version 0.11.5)