##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926685_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 698145 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.432910068825244 27.0 7.0 31.0 2.0 31.0 2 16.462150412879847 18.0 2.0 30.0 2.0 31.0 3 20.031430433505932 24.0 15.0 31.0 2.0 31.0 4 22.121831424704038 28.0 2.0 35.0 2.0 37.0 5 22.683896611735385 29.0 2.0 35.0 2.0 37.0 6 23.52250750202322 31.0 2.0 35.0 2.0 37.0 7 23.29906681276812 30.0 7.0 35.0 2.0 37.0 8 23.750653517535756 31.0 10.0 35.0 2.0 37.0 9 26.541483502710754 32.0 17.0 37.0 2.0 39.0 10 27.280434580208983 32.0 24.0 37.0 2.0 39.0 11 24.329216709995773 30.0 8.0 37.0 2.0 39.0 12 25.495825365790775 31.0 16.0 37.0 2.0 39.0 13 22.065931862292217 28.0 2.0 37.0 2.0 39.0 14 25.497217626710782 31.0 17.0 38.0 2.0 40.0 15 26.65755394652973 31.0 17.0 38.0 2.0 40.0 16 27.026352691776065 32.0 18.0 39.0 2.0 40.0 17 26.352310766388072 31.0 17.0 38.0 2.0 40.0 18 26.45355764203711 31.0 17.0 38.0 2.0 40.0 19 26.84979481339836 31.0 18.0 39.0 2.0 40.0 20 27.12357031848685 31.0 21.0 39.0 2.0 40.0 21 24.933516676335145 30.0 8.0 38.0 2.0 40.0 22 25.89263834876709 31.0 10.0 38.0 2.0 40.0 23 25.891001153055598 31.0 2.0 38.0 2.0 40.0 24 24.915609221580045 31.0 2.0 39.0 2.0 40.0 25 24.719002499480766 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.756175653263181 1101 2 10.23749826688362 1101 3 6.8966244683184925 1101 4 8.484377672843895 1101 5 8.129621482838477 1101 6 7.613784699506535 1101 7 7.471887063255739 1101 8 5.8944500605127 1101 9 5.936343426879045 1101 10 5.421436858185466 1101 11 8.110625411110284 1101 12 5.584069746097377 1101 13 10.537304333488809 1101 14 7.898745755103665 1101 15 7.062228754038145 1101 16 6.505615326026668 1101 17 2.6773375596362463 1101 18 4.991865925786353 1101 19 5.647521263679376 1101 20 3.4255844912012563 1101 21 0.8767793479614987 1101 22 4.604235561917825 1101 23 5.670840692150495 1101 24 6.774240702694684 1101 25 7.3697365162140684 1102 1 6.056909347756207 1102 2 11.172675789669384 1102 3 7.074069050212163 1102 4 8.34476954532893 1102 5 8.374898680011764 1102 6 7.316264473141878 1102 7 7.21798753390085 1102 8 7.474489589014453 1102 9 6.395656085952826 1102 10 5.837838342637113 1102 11 8.707612429267797 1102 12 6.01318860176147 1102 13 10.79323574158504 1102 14 8.365058371520785 1102 15 5.914110623537212 1102 16 6.516280912058033 1102 17 7.402711160853382 1102 18 7.573170935099721 1102 19 6.267187404247277 1102 20 6.615823226289198 1102 21 5.710283307921117 1102 22 6.76614032382259 1102 23 7.182653742243627 1102 24 8.624134771997532 1102 25 8.3795731412194 1103 1 5.256055810279019 1103 2 9.522427473408246 1103 3 6.42203548669681 1103 4 8.069954175007528 1103 5 7.523241817394162 1103 6 7.06524613805292 1103 7 6.891925958403892 1103 8 6.387034147947844 1103 9 5.64100223318551 1103 10 4.962485591940279 1103 11 6.15291132633034 1103 12 4.688056857594411 1103 13 8.97813562757775 1103 14 7.200565646168545 1103 15 6.190277753313346 1103 16 6.049437671361044 1103 17 3.6805869550391535 1103 18 2.4245034043473765 1103 19 4.402801172397773 1103 20 4.972486872990864 1103 21 7.432696505601669 1103 22 3.217606307312483 1103 23 5.383617963605619 1103 24 6.9271998197317615 1103 25 2.4400728659546473 1104 1 5.692772291918644 1104 2 10.483352608620315 1104 3 7.032520826861909 1104 4 7.6864785131800275 1104 5 7.573248933818871 1104 6 7.084064811551354 1104 7 7.059709192107281 1104 8 7.22277639104491 1104 9 6.1628980487277865 1104 10 4.883950863787703 1104 11 8.035793478337176 1104 12 4.998425488394297 1104 13 9.339272120603656 1104 14 7.0768900128067465 1104 15 6.296682535550751 1104 16 5.91810832322555 1104 17 6.302554646470977 1104 18 3.9182174664025986 1104 19 4.689348994771812 1104 20 3.9594780486242236 1104 21 3.116569263927886 1104 22 5.146140323822589 1104 23 5.4696292075566575 1104 24 6.554891963198884 1104 25 5.599708505009588 1105 1 3.4481811226627137 1105 2 9.494393764248553 1105 3 6.095683814051881 1105 4 6.077034522991966 1105 5 2.1533048073538943 1105 6 4.388117686994253 1105 7 5.342758388073484 1105 8 2.8824895281394873 1105 9 3.508098375792379 1105 10 3.132684169865655 1105 11 6.5246610171760935 1105 12 3.726627995889526 1105 13 4.753079016215537 1105 14 3.0788367285331013 1105 15 3.720394718706924 1105 16 4.026770083923285 1105 17 4.712300490025083 1105 18 2.036389311592025 1105 19 1.7563011615747541 1105 20 1.3653379559559227 1105 21 2.215837456895997 1105 22 3.530926369066524 1105 23 0.8212107011516423 1105 24 3.839963508579096 1105 25 4.62794508173349 1106 1 6.253504775995076 1106 2 10.918522460000357 1106 3 7.487308512636858 1106 4 8.83440844948576 1106 5 8.651858275644773 1106 6 7.622557380553477 1106 7 7.449714499962909 1106 8 6.996157495078883 1106 9 6.080859832167274 1106 10 5.402098422174113 1106 11 7.725857172604691 1106 12 5.230325700708523 1106 13 4.521209487907264 1106 14 5.657107634887424 1106 15 5.6433385865061645 1106 16 5.37483868415973 1106 17 5.919789253689661 1106 18 5.946407487634023 1106 19 5.602995491587098 1106 20 5.350454694059469 1106 21 5.750454614246358 1106 22 5.791087245266326 1106 23 6.228806489934577 1106 24 7.36321998867659 1106 25 6.526247783141223 1107 1 4.678263040108504 1107 2 -14.059980724713018 1107 3 -5.431934791266274 1107 4 2.4641534930673963 1107 5 5.208197981821016 1107 6 5.689151966284527 1107 7 5.298796749093068 1107 8 6.149764189382267 1107 9 4.546952755083375 1107 10 3.8067056237769776 1107 11 6.466030807379813 1107 12 3.8437660622746925 1107 13 5.68663267303058 1107 14 5.01121355799312 1107 15 4.769088006186308 1107 16 4.501878771066789 1107 17 5.238303506755237 1107 18 -0.0848068028384823 1107 19 2.731531215206246 1107 20 3.119601406092716 1107 21 0.06516540230278522 1107 22 3.2686879237153192 1107 23 0.14790823678701415 1107 24 3.731555277760428 1107 25 4.747630199833939 1108 1 5.3764562950707315 1108 2 10.605109897824413 1108 3 0.36407717043952914 1108 4 6.18131696865678 1108 5 7.430006223022968 1108 6 7.394994677587604 1108 7 7.3508892551490455 1108 8 7.040339432810082 1108 9 5.457255810745121 1108 10 4.580249130139162 1108 11 6.8597926903151105 1108 12 2.381226906834012 1108 13 2.8769060843546406 1108 14 4.770461722814623 1108 15 4.6344287924459415 1108 16 4.639684367354782 1108 17 -0.07777829285132043 1108 18 3.3071634868911737 1108 19 4.642577915181583 1108 20 4.73103045209152 1108 21 4.6998750164889564 1108 22 1.647148992065297 1108 23 4.484564195736329 1108 24 5.327884476989272 1108 25 5.628550112574601 1109 1 3.312106762908403 1109 2 -14.059980724713018 1109 3 -5.854515578143777 1109 4 1.2902327793916335 1109 5 0.5874411977551617 1109 6 2.2349408070007293 1109 7 3.460371876976449 1109 8 3.8326882226717593 1109 9 2.886037980468398 1109 10 2.2424008069048824 1109 11 0.9881313622962189 1109 12 1.3050523370859928 1109 13 2.4358961251542794 1109 14 3.0919269866406474 1109 15 3.025180829066109 1109 16 2.9651845462062063 1109 17 3.4820569217838333 1109 18 3.6279415847188687 1109 19 2.146883124464644 1109 20 0.9445178665991953 1109 21 0.5179515164876136 1109 22 2.760452041683795 1109 23 3.050889162050403 1109 24 4.19897899391561 1109 25 4.645044113655775 1110 1 3.5597986510619712 1110 2 9.527333937560456 1110 3 6.594138070134836 1110 4 7.774369781713801 1110 5 8.369528450567909 1110 6 3.879852840107091 1110 7 6.39307959298592 1110 8 7.376476281215147 1110 9 5.2924202892549665 1110 10 5.133496443139705 1110 11 3.118931863839645 1110 12 5.778752231941798 1110 13 10.304575525326335 1110 14 7.850521259374563 1110 15 7.072300327972499 1110 16 6.6943960772068785 1110 17 7.200257831534135 1110 18 6.9084165328496105 1110 19 5.671132355562587 1110 20 6.1837504264386105 1110 21 3.858982772988842 1110 22 6.045657072861577 1110 23 6.573732722109103 1110 24 7.33459954145259 1110 25 7.9670341502594475 1111 1 5.069958787095491 1111 2 9.472173616122994 1111 3 7.259171416358157 1111 4 7.9189543974244 1111 5 8.152375407559493 1111 6 7.554311328164431 1111 7 7.436909084926146 1111 8 7.463706187400753 1111 9 5.729650460303347 1111 10 5.143696308589419 1111 11 6.868414057115309 1111 12 6.075247567708338 1111 13 8.134210491450386 1111 14 5.903387869184023 1111 15 5.1749783554864415 1111 16 5.131945407898645 1111 17 6.4471178833412885 1111 18 6.383793565009245 1111 19 5.33742777290686 1111 20 4.750689195462382 1111 21 7.458482447207629 1111 22 6.718980623929767 1111 23 6.74861366629192 1111 24 6.794031834581521 1111 25 7.066696715084614 1112 1 -2.788338819192468 1112 2 7.629561758947112 1112 3 6.1465077549664855 1112 4 -0.4587829247284674 1112 5 4.291876384799263 1112 6 3.1777903017474323 1112 7 5.804169976421008 1112 8 6.163380966208312 1112 9 4.783682362453273 1112 10 3.4863528245720197 1112 11 -0.5214941033621727 1112 12 3.864765357675342 1112 13 8.161347284002346 1112 14 5.6775272677087045 1112 15 3.762859006138985 1112 16 3.9902325062320827 1112 17 5.072832424248755 1112 18 5.239671714768612 1112 19 4.771587556863317 1112 20 4.80147151267651 1112 21 -1.7807836772485857 1112 22 2.6133625460448116 1112 23 4.119906985334435 1112 24 5.906640329211957 1112 25 5.790558178212201 1113 1 -19.052227708081357 1113 2 -14.059980724713018 1113 3 -7.384012334206135 1113 4 -6.331996939016355 1113 5 -0.9163936157073991 1113 6 -0.46862252694993956 1113 7 -20.889457474559386 1113 8 -9.604409224803495 1113 9 -5.066852166603827 1113 10 -4.304186517779911 1113 11 -5.528207382989269 1113 12 -3.4275219706926983 1113 13 -2.294864399637518 1113 14 -2.62868061596156 1113 15 -2.789966904587061 1113 16 -3.19737832621459 1113 17 -2.9776628384558066 1113 18 -3.568860483640467 1113 19 -3.170532572842312 1113 20 -4.049221348447265 1113 21 -1.2100155767267218 1113 22 -2.1528949905615633 1113 23 -2.5133832816767594 1113 24 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24 2.7058784275138983 1203 25 1.1446360412414727 1204 1 3.4859114369312145 1204 2 -14.059980724713018 1204 3 -6.176952489706963 1204 4 0.611164179567286 1204 5 3.552006966788973 1204 6 3.6565347752283586 1204 7 3.8783547634959383 1204 8 4.4094521384603915 1204 9 2.572269381502302 1204 10 2.0583228950813606 1204 11 4.977985431396686 1204 12 2.4027353514845444 1204 13 1.9671521150155016 1204 14 2.7662494707558913 1204 15 2.818224865810599 1204 16 2.4984044953406617 1204 17 3.094754283241027 1204 18 3.116414588503744 1204 19 1.835667100387905 1204 20 2.2355607532903328 1204 21 3.5577721138859744 1204 22 0.1679285328195128 1204 23 1.5570635467575542 1204 24 3.242836221371558 1204 25 1.2640281473677888 1205 1 2.9915324215782846 1205 2 3.0413158717213413 1205 3 2.950864965165529 1205 4 -19.763521486819972 1205 5 -6.533301579417218 1205 6 -0.042877381857614694 1205 7 3.162406382815007 1205 8 4.02505218791147 1205 9 2.8925901070746214 1205 10 2.3578014850734945 1205 11 5.257697854350141 1205 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6.1310822231294395 2204 5 1.59206568480257 2204 6 4.227330691259056 2204 7 5.279176589521558 2204 8 5.239984092000505 2204 9 3.6045169357429607 2204 10 3.3957988120732594 2204 11 4.776999211895351 2204 12 4.929018518186314 2204 13 2.8747878597829697 2204 14 4.3971359374835295 2204 15 4.501067021216855 2204 16 4.446427603720206 2204 17 -1.194757045490796 2204 18 2.638199197712325 2204 19 3.601756246036569 2204 20 3.8597591053976927 2204 21 5.388668758025695 2204 22 4.937022842091277 2204 23 3.5586834515139394 2204 24 5.286287410079154 2204 25 5.46232795413831 2205 1 6.101367063160474 2205 2 10.543777445221622 2205 3 7.253533898757336 2205 4 7.994648447820552 2205 5 8.19138618872083 2205 6 7.905731478218019 2205 7 7.876882394721662 2205 8 7.369263319149486 2205 9 6.2510026238911784 2205 10 5.587660014114505 2205 11 7.908244458729332 2205 12 7.179307841335476 2205 13 10.359059702303 2205 14 7.741252757904785 2205 15 6.423806718557287 2205 16 6.195297865709204 2205 17 6.854695169346794 2205 18 6.797678250716327 2205 19 5.573875138562659 2205 20 6.044118571500039 2205 21 0.024771878306971473 2205 22 4.335584768267033 2205 23 5.588861233700449 2205 24 7.391934446859015 2205 25 7.9400679821337725 2206 1 4.421372291918644 2206 2 9.327219275286982 2206 3 6.163387493528578 2206 4 1.7428785131800275 2206 5 5.774515600485536 2206 6 6.227398144884688 2206 7 6.202542525440613 2206 8 6.359209724378246 2206 9 4.932498048727783 2206 10 4.2135175304543715 2206 11 4.052393478337173 2206 12 4.80995882172763 2206 13 8.331038787270323 2206 14 0.8544566794734116 2206 15 2.6574825355507485 2206 16 3.795974989892212 2206 17 4.939387979804312 2206 18 -0.25634920026406505 2206 19 1.7868489947718125 2206 20 3.2032113819575585 2206 21 3.929969263927884 2206 22 -1.4287930095107448 2206 23 -2.019337459110009 2206 24 1.9747919631988857 2206 25 3.6471751716762526 2207 1 1.4049151490614982 2207 2 -14.059980724713018 2207 3 -6.249281894226526 2207 4 -4.194541894983239 2207 5 1.1796094780365571 2207 6 1.696785899986729 2207 7 3.0933996682977565 2207 8 3.091160744786407 2207 9 0.4433062119930895 2207 10 0.39781140800539205 2207 11 -1.5039003992138475 2207 12 -2.4348738313335936 2207 13 0.6391530729846089 2207 14 -5.150882096036792 2207 15 -1.3792358317961906 2207 16 -0.35563317337309286 2207 17 0.8825716532737005 2207 18 0.7128263099400165 2207 19 0.24154287232283167 2207 20 0.15199505542694425 2207 21 -3.758634817704767 2207 22 -4.698621580939317 2207 23 -1.5990272550283748 2207 24 0.10871849381112852 2207 25 0.7920894573905386 2208 1 5.034561846450131 2208 2 6.522968584042744 2208 3 6.566860304588484 2208 4 8.366278820399689 2208 5 4.00224278942563 2208 6 6.237720725529847 2208 7 6.674290605317726 2208 8 6.85874029273462 2208 9 5.094787449649441 2208 10 4.662134427535783 2208 11 6.832636796309522 2208 12 6.176190465352821 2208 13 8.852855069912412 2208 14 3.5058569866930718 2208 15 4.947834609283468 2208 16 5.280965466082687 2208 17 5.897397196394174 2208 18 0.9777067137144293 2208 19 3.483746076185021 2208 20 4.414009538639586 2208 21 7.127298910625274 2208 22 6.053390707847164 2208 23 2.2004241384322363 2208 24 3.4700570937672417 2208 25 4.711690071829864 2209 1 3.3545942553129855 2209 2 5.360984333523255 2209 3 5.444786495192471 2209 4 0.5534502269903427 2209 5 5.183384818455586 2209 6 5.913288327912973 2209 7 6.320193107803345 2209 8 4.843360140351624 2209 9 4.555011193486518 2209 10 3.51646195641111 2209 11 -0.781186554940696 2209 12 3.250186442359908 2209 13 2.395235792262003 2209 14 3.7920300571772394 2209 15 4.26407022273877 2209 16 3.92956700320336 2209 17 1.3137041195713657 2209 18 3.368193894577864 2209 19 3.961025367483959 2209 20 4.21361005084275 2209 21 6.430604205691612 2209 22 5.075069885663964 2209 23 0.5577420749998083 2209 24 -3.296360116668005 2209 25 0.06321377400570327 2210 1 -1.9667960534050977 2210 2 -14.059980724713018 2210 3 -7.755165024457035 2210 4 -1.9973344364602603 2210 5 -0.6811275649820878 2210 6 -1.2396522148275437 2210 7 -1.2455725824730557 2210 8 -21.306390275621755 2210 9 -13.27242569227941 2210 10 -7.233177433574404 2210 11 -10.313630982094482 2210 12 -4.94503973942345 2210 13 -5.6380720040965855 2210 14 -4.141029651461842 2210 15 -5.960691565168673 2210 16 -5.339109902194117 2210 17 -4.8949933151597165 2210 18 -4.922927617530252 2210 19 -10.476496328969194 2210 20 -7.2568289058122275 2210 21 -5.730265268446214 2210 22 -10.568991570661824 2210 23 -8.420760480692742 2210 24 -11.127644008024136 2210 25 -8.51348094343166 2211 1 0.2047652989116493 2211 2 5.349984310252015 2211 3 0.8968448361859203 2211 4 2.0555344572359715 2211 5 1.4458596564295938 2211 6 0.4613841588707004 2211 7 -20.889457474559386 2211 8 -13.394676988908468 2211 9 -8.9096194337897 2211 10 -7.054679672342832 2211 11 -9.659765962222266 2211 12 -4.827856562887753 2211 13 -6.862225548394013 2211 14 -4.620446817030086 2211 15 -5.049820960952747 2211 16 -11.89365158353436 2211 17 -7.900458174041841 2211 18 -5.797172976487843 2211 19 -8.454479676556858 2211 20 -6.545824981678805 2211 21 -4.393185980827358 2211 22 -5.699186016503752 2211 23 -7.914572424144975 2211 24 -22.547608036801115 2211 25 -22.333624828323746 2212 1 -0.6280928367415157 2212 2 -14.059980724713018 2212 3 -7.612405230517563 2212 4 -19.763521486819972 2212 5 -20.235084399514463 2212 6 -21.052601855115313 2212 7 -9.696910890708454 2212 8 -9.31969994731652 2212 9 -4.933978614981179 2212 10 -5.273244492615728 2212 11 -5.3063848712635355 2212 12 -2.5703485429573725 2212 13 -5.950696084069518 2212 14 -3.320039150162792 2212 15 -3.3840628060641578 2212 16 -3.7094219932488706 2212 17 -3.9238524815976383 2212 18 -2.727138197602134 2212 19 -3.432884811794292 2212 20 -3.69095081857483 2212 21 -1.4425744805264173 2212 22 -2.747317233113229 2212 23 -2.85847712193166 2212 24 -1.779196324290023 2212 25 -2.1934296198055563 2213 1 -19.052227708081357 2213 2 -14.059980724713018 2213 3 -8.30865654316867 2213 4 -5.222237083150247 2213 5 -20.235084399514463 2213 6 -10.36636332300522 2213 7 -20.889457474559386 2213 8 -10.472445321493314 2213 9 -7.833065254024511 2213 10 -6.874130175967647 2213 11 -6.27562854001145 2213 12 -4.674892554419159 2213 13 -19.52656121272968 2213 14 -12.236787357223836 2213 15 -6.698730308485949 2213 16 -5.8053167532270535 2213 17 -4.278263396342478 2213 18 -4.343196906686085 2213 19 -5.107462931833691 2213 20 -5.263151920794737 2213 21 -3.3502655984574368 2213 22 -4.2121471379511135 2213 23 -3.9213264499356946 2213 24 -2.917332807443316 2213 25 -3.8804138191494353 2214 1 -19.052227708081357 2214 2 -14.059980724713018 2214 3 -12.35215616281508 2214 4 -19.763521486819972 2214 5 -20.235084399514463 2214 6 -21.052601855115313 2214 7 -16.550796135898047 2214 8 -15.19050615973764 2214 9 -14.232047006217272 2214 10 -13.854005346668506 2214 11 -14.743432295888601 2214 12 -12.519914504945696 2214 13 -19.52656121272968 2214 14 -17.07599127257454 2214 15 -24.30331746444925 2214 16 -18.62929094417372 2214 17 -23.996612020195688 2214 18 -18.162932217247082 2214 19 -15.407276879354061 2214 20 -13.952043762897587 2214 21 -11.425653513294892 2214 22 -12.492267934585671 2214 23 -12.40570629027884 2214 24 -11.30285279204587 2214 25 -10.984973479672398 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 121729.0 3 3.0 4 470.0 5 1968.0 6 2765.0 7 2703.0 8 2321.0 9 2265.0 10 2011.0 11 1932.0 12 1911.0 13 2031.0 14 3195.0 15 7048.0 16 10763.0 17 11441.0 18 11819.0 19 13605.0 20 15826.0 21 18336.0 22 19050.0 23 20779.0 24 21696.0 25 22056.0 26 18485.0 27 14346.0 28 15545.0 29 17819.0 30 17350.0 31 20125.0 32 25936.0 33 33261.0 34 40533.0 35 46761.0 36 46195.0 37 48592.0 38 35474.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.592821852084434 19.610491445962875 13.109988071861222 25.68669863009147 2 12.520591957718604 20.975962226680814 43.937841258444124 22.56560455715646 3 18.686772517400172 25.62490574430391 29.702388115632505 25.98593362266341 4 10.961610603976492 18.35471635196532 39.54833062398399 31.135342420074196 5 11.424286408639665 38.59818497779494 36.92481573463124 13.052712878934159 6 29.3522243246031 39.52346409240556 19.003944923257464 12.120366659733882 7 25.9104942423058 31.398383066666458 23.621500244486395 19.06962244654135 8 22.6925542433099 39.96038946223107 21.83499851770911 15.512057776749918 9 26.33389348352241 15.327802567131993 21.04649628924397 37.291807660101625 10 14.156646660595992 29.7314163192515 36.16293649939342 19.949000520759085 11 32.46398890911723 21.748642204769993 26.933629902726253 18.853738983386528 12 23.981946663089737 24.34975063104369 33.715719389808186 17.952583316058384 13 28.57853019492359 22.275163583071304 25.622338119843953 23.523968102161156 14 18.917033137509957 24.120360726755745 28.405848190029758 28.556757945704536 15 21.002829554437525 34.72712899960844 23.542188435027374 20.727853010926662 16 19.632300972625142 28.16502393858474 31.58074503854718 20.621930050242934 17 19.859490727747037 29.658358026843295 30.54865384672199 19.933497398687674 18 19.70360969163124 27.45176234632139 33.87442563825653 18.97020232379084 19 22.706999234817825 26.58295243941823 27.876779484971298 22.83326884079265 20 22.855644929617927 28.626544096523986 29.360241309968398 19.15756966388969 21 20.859287341853907 26.815028215831205 27.118740809389557 25.206943632925327 22 21.96465772183617 30.399749428200984 28.076278717422028 19.559314132540827 23 20.13750483919533 29.66978764893968 29.781626829932755 20.411080681932237 24 22.30116797976912 27.628822690915523 30.2828629474638 19.78714638185156 25 21.412235358220833 28.374187165276993 29.85481893591242 20.358758540589758 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 695.0 1 695.0 2 1575.0 3 2455.0 4 2455.0 5 2455.0 6 3520.0 7 4585.0 8 4585.0 9 4585.0 10 5256.5 11 5928.0 12 5928.0 13 5928.0 14 7494.5 15 9061.0 16 9061.0 17 9061.0 18 13470.5 19 17880.0 20 17880.0 21 17880.0 22 25641.5 23 33403.0 24 33403.0 25 33403.0 26 43945.5 27 54488.0 28 54488.0 29 54488.0 30 65561.0 31 76634.0 32 76634.0 33 76634.0 34 82130.0 35 87626.0 36 87626.0 37 87626.0 38 88568.5 39 89511.0 40 89511.0 41 89511.0 42 87686.5 43 85862.0 44 85862.0 45 85862.0 46 82409.5 47 78957.0 48 78957.0 49 78957.0 50 69881.0 51 60805.0 52 60805.0 53 60805.0 54 50447.5 55 40090.0 56 40090.0 57 40090.0 58 32344.5 59 24599.0 60 24599.0 61 24599.0 62 19148.5 63 13698.0 64 13698.0 65 13698.0 66 10411.0 67 7124.0 68 7124.0 69 7124.0 70 5067.5 71 3011.0 72 3011.0 73 3011.0 74 2079.0 75 1147.0 76 1147.0 77 1147.0 78 780.0 79 413.0 80 413.0 81 413.0 82 267.0 83 121.0 84 121.0 85 121.0 86 82.0 87 43.0 88 43.0 89 43.0 90 25.5 91 8.0 92 8.0 93 8.0 94 4.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.780375136970113 2 36.96166269184768 3 5.970965916822436 4 14.090196162688267 5 13.948535046444507 6 15.290806351116174 7 9.48341676872283 8 7.7164485887602146 9 0.1021277814780597 10 1.5307708284095711 11 5.980133066913034 12 6.0286903150491655 13 16.728473311418117 14 1.6675618961677015 15 3.059536342736824 16 3.1270008379348133 17 3.0336104963868538 18 1.5867763859943134 19 1.5365002972161943 20 0.2785954207220563 21 4.638577945842196 22 1.678018176739789 23 0.10241425491839089 24 9.261399852466178 25 0.10599517292253043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 698145.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.02539699723873 #Duplication Level Percentage of deduplicated Percentage of total 1 96.38250443240982 85.80490720683406 2 2.4640415879471242 4.387245611693984 3 0.4838175117642982 1.292161381770986 4 0.2077597889974589 0.7398359078224531 5 0.10885621948205598 0.4845484077504293 6 0.07147151619126245 0.3817668061753029 7 0.04623069533385128 0.28809942038881514 8 0.037744436211874295 0.268817073455925 9 0.018881349532129006 0.15128276741472713 >10 0.15198681605035894 2.508647813230853 >50 0.014948609890659399 0.9242327211946854 >100 0.01074929023092094 1.9012100369267495 >500 6.718306387976579E-4 0.49672196141779024 >1k 3.3591531939882894E-4 0.37052288392332455 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1309 0.18749686669674637 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1169 0.16744372587356493 No Hit CTGTAGGACGTGGAATATGGCAAGA 961 0.13765048807912397 No Hit CTTTAGGACGTGAAATATGGCGAGG 930 0.13321014975399093 No Hit GTCCTACAGTGTGCATTTCTCATTT 844 0.1208917918197509 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACGA 25 0.0034933798 20.877806 5 TGTAGGG 50 0.0020076227 16.472057 2 GTGTAGG 80 1.5133932E-5 15.064707 1 TCTAGAC 45 0.0044162176 14.635328 3 TGTAGGA 840 0.0 14.543781 2 GTGTTAT 65 8.1190816E-4 14.420916 1 GTATAAA 95 4.885418E-6 14.095632 1 CTGTAGG 810 0.0 13.721489 1 GTCCTAA 460 0.0 13.681956 1 GACATGC 45 0.0071529793 13.608936 7 CTATAGT 135 4.7293724E-11 13.522784 4 TGTAGAA 235 0.0 13.434656 2 GTGTTAG 70 0.0014212397 13.390851 1 GTAGAGC 50 0.008874874 13.171796 3 GTAAGAC 50 0.008874874 13.171796 3 GGACCTG 700 0.0 13.11798 6 TCCTAAA 545 0.0 12.845182 2 TAGGACC 405 0.0 12.727326 4 CTAGAGT 120 2.3088432E-8 12.528462 4 AGGACCT 750 0.0 12.526684 5 >>END_MODULE