FastQCFastQC Report
Thu 26 May 2016
SRR1926685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698145
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA28810.4126649907970408No Hit
GTCCTACAGTGGACATTTCTAAATT27660.3961927679779988No Hit
CTTTAGGACGTGAAATATGGCGAGG26650.38172585924127506No Hit
GTCCTAAAGTGTGTATTTCTCATTT25080.3592376941752788No Hit
GTCCTACAGTGTGCATTTCTCATTT18770.26885532375079674No Hit
CTGTAGGACCTGGAATATGGCGAGA16630.23820266563536227No Hit
CTGAAGGACCTGGAATATGGCGAGA14300.20482850983678177No Hit
CCCATGTACTCTGCGTTGATACCAC13560.19422899254452872No Hit
GTACATGGGAAGCAGTGGTATCAAC12260.1756082189230031No Hit
GTATCAACGCAGAGTACTTTTTTTT9470.1356451739968058No Hit
GAATATGGCAAGAAAACTGAAAATC9110.1304886520708449No Hit
GTCCTTCAGTGTGCATTTCTCATTT8840.1266212606263742No Hit
GGAATATGGCGAGAAAACTGAAAAT8230.1178838206962737No Hit
CTGTAGGACATGGAATATGGCAAGA7430.10642488308302717No Hit
GCCATATTCCACGTCCTACAGTGGA7010.10040894083607273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC902.1373125E-914.8455413
TAGGACC6800.014.3132754
TCTATAC706.9140624E-613.6336583
CTAGGGT500.001448890113.3590574
AATACGG500.001526290613.26713814
ATACGGC500.001532897913.25953415
GGCGAGG7800.012.99240119
GGACCTG11550.012.4741626
AGGACCT11950.012.4567425
TAGCCTG701.0450837E-412.2685225
TATACTG701.0450837E-412.2685225
GGTTTAA550.002967037712.1437211
GCCTCGA550.003136289812.05412216
TAGGTCC657.721162E-411.7442265
TGTAGGA17000.011.7372022
GATATAC4000.011.6883311
CTGTAGG16750.011.677651
TGGCGAG19000.011.66523818
TCGAACT658.2211674E-411.656733519
ATTACAC1001.8212595E-611.4522733
Produced by FastQC (version 0.11.5)