FastQCFastQC Report
Thu 26 May 2016
SRR1926684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656225
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11620.17707341232046936No Hit
GTCCTAAAGTGTGTATTTCTCATTT9610.14644367404472552No Hit
CTGTAGGACGTGGAATATGGCAAGA9100.13867194940759647No Hit
CTTTAGGACGTGAAATATGGCGAGG7950.12114747228465846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3150.017.0239434
GTGTAGG1003.113928E-815.9900721
GTCTTAG500.00244787115.9900721
GTATAAT550.004617120614.5364291
CTCCTAG550.004617120614.5364291
ATGTCGA451.4044883E-414.36876817
TTATCCC450.00515033714.3001134
CTGTAGG6650.014.2267571
TGTAGGA7300.013.8647222
TTTAGGA5000.013.67736152
TCCTAGG600.006914295313.67736152
TATGTCG554.4422668E-513.44126816
GTATAAA904.810051E-513.3250611
GTAGGAC7350.013.2661693
ATGGGTA550.001530696513.2606355
AGGACCT5750.013.0464895
TTAGGAC5300.013.0228953
GGACCTG5500.012.8736566
TAGCACA756.669621E-512.87014
AGGACGT7650.012.5301255
Produced by FastQC (version 0.11.5)