FastQCFastQC Report
Thu 26 May 2016
SRR1926684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656225
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23060.35140386300430493No Hit
CTGTAGGACGTGGAATATGGCAAGA22700.34591793973103735No Hit
CTTTAGGACGTGAAATATGGCGAGG21610.32930778315364395No Hit
GTCCTAAAGTGTGTATTTCTCATTT20890.31833593660710885No Hit
GTCCTACAGTGTGCATTTCTCATTT14370.21897977065792984No Hit
CTGTAGGACCTGGAATATGGCGAGA11840.18042592098746618No Hit
CTGAAGGACCTGGAATATGGCGAGA10880.1657967922587527No Hit
CCCATGTACTCTGCGTTGATACCAC10610.16168234980380203No Hit
GTACATGGGAAGCAGTGGTATCAAC9440.1438530991656825No Hit
GTCCTTCAGTGTGCATTTCTCATTT7390.1126138138595756No Hit
GAATATGGCAAGAAAACTGAAAATC6830.10408015543449274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG350.0021695616.2844059
AGGTTAC400.005280274414.2477667
TAGGACC4400.014.0878544
CTTACAC500.001453690713.3533
GTCCTAT1001.3703175E-712.395411
AGTACTG550.002978624312.1372285
CGTCAAG802.746295E-511.922323
ACTGTTC2400.011.8740458
GTATTAA657.7695056E-411.73531
TTCTATA907.1013474E-611.6600642
CTGTAGG13300.011.4705931
TGTAGGA13150.011.4625712
ATAGGAC1001.8338633E-611.4454283
TTAGGAC8300.011.2615663
AGGACGT12750.011.2192865
GTATAAA855.1167233E-511.2175661
CCAACGA855.4584474E-511.14661719
TAGGACT1201.2046075E-711.1257934
GGACCTG8400.011.1249396
GACGTGA7700.011.1021567
Produced by FastQC (version 0.11.5)