FastQCFastQC Report
Thu 26 May 2016
SRR1926683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594833
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14280.24006738025630722No Hit
GTCCTAAAGTGTGTATTTCTCATTT11270.18946494226110522No Hit
CTGTAGGACGTGGAATATGGCAAGA10060.1691230984158579No Hit
CTTTAGGACGTGAAATATGGCGAGG9220.15500148781254572No Hit
GTCCTACAGTGTGCATTTCTCATTT8150.1370132457345171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTAC300.00797438218.0990093
GTTTAGA602.3698396E-517.6888831
GTTCCAT500.002520171215.9199941
AGGACCG400.002995082215.5144855
TATACCA400.002995082215.5144855
TAATCCT506.2808365E-414.9183174
TGTAGGA8900.014.6671152
CTGTAGG8350.014.29939651
TAAGGCT450.005375302414.2079214
TAGGACC3300.014.2079214
GGACCTG5750.014.0289076
GTCCTAC10700.013.88661
CCTGGAC550.001119783713.8210613
AGACTGA601.9904286E-413.7890986
GTCCTAA4700.013.5489321
AGGACCT6100.013.3950215
GTCATAA802.7580894E-413.2666621
GCTATAT600.00845770113.2666621
GATATAC1401.0324584E-813.2666621
GTAGGAC9300.012.96122553
Produced by FastQC (version 0.11.5)