FastQCFastQC Report
Thu 26 May 2016
SRR1926683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594833
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA27750.46651749314513485No Hit
GTCCTACAGTGGACATTTCTAAATT26690.44869736547905037No Hit
CTTTAGGACGTGAAATATGGCGAGG26290.44197278900128273No Hit
GTCCTAAAGTGTGTATTTCTCATTT25310.42549757663075183No Hit
GTCCTACAGTGTGCATTTCTCATTT17670.2970581659053886No Hit
CTGAAGGACCTGGAATATGGCGAGA15410.259064308806001No Hit
CTGTAGGACCTGGAATATGGCGAGA14180.2383862361368653No Hit
CCCATGTACTCTGCGTTGATACCAC10010.16828252635613694No Hit
GTACATGGGAAGCAGTGGTATCAAC9930.16693761106058339No Hit
GAATATGGCAAGAAAACTGAAAATC8870.14911748339449896No Hit
GTCCTTCAGTGTGCATTTCTCATTT8330.14003930514951254No Hit
GGAATATGGCGAGAAAACTGAAAAT7790.13096112690452613No Hit
CTGTAGGACATGGAATATGGCAAGA7280.12238729189537233No Hit
GCCATATTCCACGTCCTACAGTGGA6750.11347722806233008No Hit
CCATATTCCAGGTCCTTCAGTGTGC6680.11230042717872074No Hit
ACAGTGGACATTTCTAAATTTTCCA6590.11078739747122301No Hit
ACCTGGAATATGGCGAGAAAACTGA6550.11011493982344624No Hit
GACCTGGAATATGGCGAGAAAACTG6300.10591207952484141No Hit
CCATATTTCACGTCCTAAAGTGTGT6100.10254979128595756No Hit
ATTCCAGGTCCTTCAGTGTGCATTT6080.1022135624620692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTGG250.00593263219.05241
TAAGGCG508.878534E-515.16374316
AAGACGT400.00515820114.2977585
TAGGACC4700.013.5879424
TAGGACT1550.013.5290614
GTATTGG500.00146687113.336681
AAGGCGA500.001524842513.26827417
TCCAATA603.9688268E-412.7101942
AGGACTT1650.012.7091195
TGGCGAA604.1744745E-412.63432818
CTATGAC701.0552168E-412.2552213
ATTACAC701.0552168E-412.2552213
CTCTAGG1404.9112714E-1112.2479711
ACTGACC550.003057582512.0946118
TCCTAGG657.783265E-411.7324872
TAAGGAC657.7887916E-411.7314943
GGCGAGG7850.011.70888319
TGTAGGA16650.011.6223852
GTCCTAA8050.011.5971141
AATCCCG907.653498E-611.58146819
Produced by FastQC (version 0.11.5)