FastQCFastQC Report
Thu 26 May 2016
SRR1926682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677375
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7040.10393061450452114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTAC250.002697296221.8294353
GACCTAC450.001204085917.7792151
TGTAGGA5350.016.0933022
CTAGGTA400.002366375616.0727634
GTCCTAC5250.015.4933141
CTGTAGG5200.015.1294271
GATATAC801.5749652E-515.0012121
TCTAGAC601.2593137E-414.55295853
GTGCTAG550.004596079214.5466291
TAGGACC2900.014.4100644
TTAATCC754.2536813E-614.2869014
AGGACGT3950.013.699085
GGACGTG3950.013.6976996
TTCTAGA700.001218580613.6665352
TAATACT550.001239169213.6374964
GTAGGAC5800.013.5493053
CAACACT952.4029214E-713.534964
CTTTAGG2700.013.3344121
GTATTAA802.6430204E-413.3344111
TCCTACA6350.013.1284812
Produced by FastQC (version 0.11.5)