FastQCFastQC Report
Thu 26 May 2016
SRR1926682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677375
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA15410.22749584794242483No Hit
GTCCTACAGTGGACATTTCTAAATT15040.22203358553238603No Hit
CTTTAGGACGTGAAATATGGCGAGG13810.2038752537368518No Hit
GTCCTAAAGTGTGTATTTCTCATTT13020.19221258534785016No Hit
GTCCTACAGTGTGCATTTCTCATTT11550.17051116442147998No Hit
CTGTAGGACCTGGAATATGGCGAGA10560.1558959217567817No Hit
GTATCAACGCAGAGTACTTTTTTTT9460.13965676324045026No Hit
CTGAAGGACCTGGAATATGGCGAGA7670.11323122347296549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3750.015.7549554
CCCTTAT551.09488865E-515.5920911
ATAGGAC1006.2573235E-1014.2969923
ATAGCAC400.00516102114.2969923
GATATAC1800.014.2927481
AGGACCT6700.013.3693355
GTCCTAC8200.012.7821341
GTAGGAC9850.012.7729483
TCCTACA9450.012.7093822
TGGATTG603.9822105E-412.7056085
TGTAGGA10300.012.6785342
CTGTAGG10250.012.456771
GGACCTG6750.012.4232626
CCTACAG9750.012.0241383
TAGGACT1209.5260475E-911.9115084
TATACAC2650.011.8692023
CTAGGGT657.809348E-411.7282544
GTCCTAA4000.011.6724121
TAGAGGT907.192866E-611.6468084
TTATCCT907.192866E-611.6468084
Produced by FastQC (version 0.11.5)