FastQCFastQC Report
Thu 26 May 2016
SRR1926681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612995
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7270.1185980309790455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA350.006016070519.0038221
ATCCTAT552.3961233E-416.9306771
TATACTG701.6094964E-716.3165455
CTAGTAC601.2775091E-414.5278933
GTGTAGG550.004668328414.5120091
TGTAGGA5000.014.458882
CTATACT450.00523048614.2666364
GTATAGA955.22767E-614.0028151
CTGTAGG4700.013.8687461
CTTGGAC550.001091818813.8675363
AGATAGC450.00639305113.8412846
TATTATA600.00703810513.6404532
CATAATA700.001235895213.6404532
TAGGACC2300.013.4912764
GTCCTAC5650.013.4203971
GTTATAC500.0093153513.0751053
ACAGCGC450.009757956512.9839118
GGTCTCG450.009757956512.9839118
GGACCTG4100.012.9129056
GTAGGAC5150.012.9058483
Produced by FastQC (version 0.11.5)