##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926681_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 612995 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.65500860529042 26.0 2.0 30.0 2.0 31.0 2 15.486424848489792 15.0 2.0 30.0 2.0 31.0 3 18.782068369236292 16.0 7.0 30.0 2.0 31.0 4 20.86967593536652 27.0 2.0 33.0 2.0 35.0 5 20.969172668618832 27.0 2.0 35.0 2.0 35.0 6 21.9254773693097 29.0 2.0 35.0 2.0 35.0 7 21.767583748643954 29.0 2.0 35.0 2.0 36.0 8 21.890185074919046 29.0 2.0 35.0 2.0 37.0 9 24.516960170963873 31.0 10.0 37.0 2.0 39.0 10 25.186141811923424 31.0 10.0 37.0 2.0 39.0 11 22.456579580583856 28.0 2.0 35.0 2.0 39.0 12 23.540094128010832 30.0 2.0 37.0 2.0 39.0 13 20.35852984118957 25.0 2.0 35.0 2.0 39.0 14 23.46454865047839 29.0 2.0 37.0 2.0 40.0 15 24.499015489522755 30.0 2.0 37.0 2.0 40.0 16 24.83917650225532 31.0 2.0 38.0 2.0 40.0 17 24.16696547280157 30.0 2.0 37.0 2.0 40.0 18 24.200145188786205 30.0 2.0 37.0 2.0 40.0 19 24.546332351813636 30.0 2.0 38.0 2.0 40.0 20 24.788897136191977 31.0 2.0 38.0 2.0 40.0 21 22.711614287229096 30.0 2.0 36.0 2.0 40.0 22 23.595761792510544 30.0 2.0 37.0 2.0 40.0 23 23.552634197668823 30.0 2.0 37.0 2.0 40.0 24 22.694906157472737 30.0 2.0 38.0 2.0 40.0 25 22.48338893465689 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.961408426173069 1101 2 9.54094645855349 1101 3 6.364339565789216 1101 4 7.70552803607578 1101 5 7.103698153616044 1101 6 6.800802987119621 1101 7 6.43540006273512 1101 8 4.817933221863061 1101 9 5.007233944776726 1101 10 4.890385960530502 1101 11 7.671971571406324 1101 12 5.004746650300323 1101 13 9.460970715093833 1101 14 6.895565384084783 1101 15 6.131261677851228 1101 16 5.6673856397319184 1101 17 1.6963917062707132 1101 18 3.8936823783975605 1101 19 4.427661931772299 1101 20 2.482485256554156 1101 21 0.26993136407327256 1101 22 3.562654555639231 1101 23 4.555247395917046 1101 24 5.760962319821903 1101 25 6.408801639062641 1102 1 4.35803665056687 1102 2 9.213329375601765 1102 3 5.7505074610080875 1102 4 6.8210389152093605 1102 5 6.734735917147162 1102 6 5.590560507370725 1102 7 5.329037970313614 1102 8 5.43757434827797 1102 9 4.7914803191398505 1102 10 4.020492751208323 1102 11 6.738443210967777 1102 12 4.3139745741508335 1102 13 8.787643487472305 1102 14 6.378847885885374 1102 15 4.034324907535655 1102 16 4.6272222082033245 1102 17 5.365334380750319 1102 18 5.403005444650461 1102 19 4.146862913428489 1102 20 4.556249961390414 1102 21 3.423245787483637 1102 22 4.833183529612892 1102 23 5.053938520998184 1102 24 6.278593896429321 1102 25 6.256187890540904 1103 1 3.871366314159012 1103 2 8.014167150664532 1103 3 5.50262181372392 1103 4 6.916709068130164 1103 5 6.058133202433737 1103 6 5.53909462477559 1103 7 5.720619046979877 1103 8 5.461769578509475 1103 9 4.469905373626556 1103 10 3.951149858646712 1103 11 4.307124207526279 1103 12 3.670352987122026 1103 13 7.423583679641435 1103 14 5.837023129453257 1103 15 4.77227137406717 1103 16 4.610848002008623 1103 17 2.4987903371430527 1103 18 0.8351666984488659 1103 19 2.9508226536824154 1103 20 3.550452665363135 1103 21 5.5560698323439865 1103 22 1.3852021110341575 1103 23 3.4616628786815937 1103 24 4.568131106262438 1103 25 1.105595137832232 1104 1 5.414650497481489 1104 2 9.75590594673776 1104 3 6.717657688785064 1104 4 7.32722805980945 1104 5 6.990824629824338 1104 6 6.791376215886213 1104 7 6.867164028787311 1104 8 7.195985467120224 1104 9 6.112038886923742 1104 10 4.841324826407547 1104 11 7.900615166173829 1104 12 5.2150209041189335 1104 13 9.0519321712671 1104 14 7.093024322236918 1104 15 6.392032346499743 1104 16 5.792151118155932 1104 17 6.3240684571485986 1104 18 3.9428128079076927 1104 19 4.748666749534092 1104 20 3.6624592361362716 1104 21 2.8873497990716004 1104 22 5.139844419802948 1104 23 5.468029623036145 1104 24 6.452499100947225 1104 25 5.376830479802784 1105 1 2.9501427205969186 1105 2 9.08633180221213 1105 3 6.270194542154989 1105 4 5.7509133797344205 1105 5 2.264518398246757 1105 6 4.7098849110115175 1105 7 5.611033747836366 1105 8 2.7813391860805297 1105 9 3.8938850286912334 1105 10 3.8657512246338754 1105 11 7.080470876078028 1105 12 4.398061441545778 1105 13 5.3704618596284845 1105 14 3.7731650622570214 1105 15 4.5059291860789905 1105 16 4.716365535900849 1105 17 5.4228450464475095 1105 18 2.977586482048295 1105 19 2.636608951570949 1105 20 2.252931549812768 1105 21 2.9409994646305755 1105 22 4.2435040699498146 1105 23 1.8714206280318493 1105 24 4.660002732094362 1105 25 5.416793579496762 1106 1 5.994854941658293 1106 2 10.221543366947325 1106 3 7.02857944397395 1106 4 8.12887856765802 1106 5 7.950181689521639 1106 6 6.848120264222157 1106 7 6.7166135249533525 1106 8 6.41663111728683 1106 9 5.79310045101704 1106 10 5.075005423781992 1106 11 7.150903716497005 1106 12 4.771137669143624 1106 13 4.0531716830901345 1106 14 5.0122653596129645 1106 15 5.153840327550768 1106 16 5.0874409732283965 1106 17 5.409755357465752 1106 18 5.535994489227697 1106 19 5.481617189134237 1106 20 4.950763664254623 1106 21 5.159969635275381 1106 22 5.591516004370774 1106 23 5.91200569594152 1106 24 6.715273098297583 1106 25 5.739111683383822 1107 1 4.516189742864473 1107 2 -12.764869676530937 1107 3 -4.73033406444141 1107 4 2.3277595636861292 1107 5 4.911912384669023 1107 6 5.290357641179874 1107 7 4.986379163444834 1107 8 5.574700598815543 1107 9 3.647639067364228 1107 10 3.3771315147236862 1107 11 5.895270505375709 1107 12 3.52446279965811 1107 13 4.750197081939749 1107 14 4.230239495826254 1107 15 4.177650088573099 1107 16 3.8535626671935113 1107 17 4.356139807312324 1107 18 -0.733631668133583 1107 19 1.9062948880941057 1107 20 2.49093921121991 1107 21 -0.6913775199737593 1107 22 2.23033210846355 1107 23 -0.5610744459307178 1107 24 2.433111904604786 1107 25 3.1642543194443427 1108 1 5.532711406154949 1108 2 10.408379874636028 1108 3 0.3871449996162397 1108 4 6.061291793271426 1108 5 7.316465221902664 1108 6 7.403029131601752 1108 7 7.458835378568736 1108 8 7.171807333038917 1108 9 5.911245990006972 1108 10 5.164206110691676 1108 11 7.427796295790841 1108 12 2.7809583310747747 1108 13 3.93153467843268 1108 14 4.963508431972656 1108 15 5.075759641681852 1108 16 5.059655606486274 1108 17 0.19196400628317534 1108 18 3.786279075390631 1108 19 4.767196964327429 1108 20 5.177160901004292 1108 21 5.044874859952092 1108 22 1.6371945228314715 1108 23 4.470236903497526 1108 24 5.24236732721544 1108 25 5.929395749160626 1109 1 3.079229265690305 1109 2 -12.764869676530937 1109 3 -5.102031090311145 1109 4 1.697286527857024 1109 5 0.9203985400515968 1109 6 2.186050179861354 1109 7 3.0616951818860834 1109 8 3.0615451341589903 1109 9 2.931565733301273 1109 10 2.0378947813420147 1109 11 0.28554301997959186 1109 12 0.8116146321546545 1109 13 2.0240780209659732 1109 14 2.935407799587008 1109 15 3.088487742649189 1109 16 2.8322630584544406 1109 17 3.325597343189308 1109 18 3.5098615668829005 1109 19 2.1612489217753783 1109 20 1.0727218083169205 1109 21 0.6565086791862633 1109 22 2.6666701250716187 1109 23 2.9122084863373665 1109 24 3.8608090057170052 1109 25 4.368099894502141 1110 1 3.661971976716181 1110 2 9.265856580452303 1110 3 6.721944353697861 1110 4 8.275875774361928 1110 5 8.991969803844363 1110 6 4.31040570669645 1110 7 6.7403514813941285 1110 8 7.783671235495881 1110 9 6.052092637884499 1110 10 5.946412597290209 1110 11 3.7362156345603204 1110 12 6.6930923691679105 1110 13 10.676552381009333 1110 14 8.509421460333556 1110 15 7.773901456204317 1110 16 7.486750745716453 1110 17 7.937556750065543 1110 18 7.854941335636898 1110 19 6.813639899845921 1110 20 6.954842281370645 1110 21 4.300236617705956 1110 22 6.643045232773332 1110 23 7.410507841516775 1110 24 8.382359771441457 1110 25 8.785732402353943 1111 1 5.313951879038658 1111 2 9.171922776299253 1111 3 7.151084229317128 1111 4 7.548768504764936 1111 5 7.740016253371788 1111 6 7.115911486535531 1111 7 7.072769527140938 1111 8 7.19514834452227 1111 9 5.731871985061339 1111 10 5.036490247407912 1111 11 6.985416295113357 1111 12 6.147234879972171 1111 13 7.749035779343895 1111 14 5.650554941237246 1111 15 5.51225569533857 1111 16 5.094981306835177 1111 17 6.3194969489341695 1111 18 6.235621916080309 1111 19 5.131996598486165 1111 20 4.513868668356345 1111 21 7.177090909543907 1111 22 6.4838765457981715 1111 23 6.740471651642913 1111 24 6.511872086539309 1111 25 6.909398120354016 1112 1 -1.6720452844893394 1112 2 7.841219762098939 1112 3 5.996474064694038 1112 4 0.12521142525395845 1112 5 4.480237605902172 1112 6 3.6528843605279526 1112 7 6.122868265090421 1112 8 6.188646899357735 1112 9 5.057463066331646 1112 10 3.920049481201424 1112 11 0.4270890955011275 1112 12 4.46011659190869 1112 13 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2204 4 5.26827558662821 2204 5 0.6229115508782215 2204 6 3.2097738717002606 2204 7 4.330751323940039 2204 8 4.085357306360315 2204 9 2.459843214459166 2204 10 2.021718043082096 2204 11 3.7032411148709876 2204 12 4.018817944859425 2204 13 2.3305419749089964 2204 14 3.4291627713407067 2204 15 3.571189410461887 2204 16 3.4127473241179906 2204 17 -1.5160674696030974 2204 18 1.6916811276158228 2204 19 2.4857190508050273 2204 20 2.780449261835237 2204 21 3.9558461719392994 2204 22 3.489588055743461 2204 23 2.2085376467682742 2204 24 3.791745448166008 2204 25 3.86936727035998 2205 1 5.69293544016697 2205 2 9.82805870062228 2205 3 7.024187600910896 2205 4 7.446402744146383 2205 5 7.438936009098022 2205 6 7.029337990581098 2205 7 6.971116231668898 2205 8 6.700922476820118 2205 9 5.744722016707449 2205 10 5.225198572900993 2205 11 7.405531247473128 2205 12 6.620281801746405 2205 13 9.473525592717372 2205 14 7.248739994009277 2205 15 6.10034820461858 2205 16 5.641652478083401 2205 17 6.497662842979569 2205 18 6.278712800289757 2205 19 5.022402695066674 2205 20 5.504870053942824 2205 21 -0.5133562414508077 2205 22 3.972850014382992 2205 23 5.137674819675208 2205 24 6.849254696573691 2205 25 7.250083900131294 2206 1 4.1495878552561365 2206 2 8.277935423651213 2206 3 5.8809790638361505 2206 4 2.2549031578219534 2206 5 5.403347524636867 2206 6 5.725441678651556 2206 7 6.075720003135029 2206 8 6.200274302524811 2206 9 4.646316773183827 2206 10 4.003947373572121 2206 11 3.9825293995571123 2206 12 4.891024617745824 2206 13 8.065833043666679 2206 14 1.0332026368759735 2206 15 2.7093859836844487 2206 16 3.4442458358244217 2206 17 4.670931804761231 2206 18 -0.8098071659556396 2206 19 1.2793571511204576 2206 20 2.582891248003733 2206 21 3.342965398322818 2206 22 -1.600953505409855 2206 23 -2.3689794945233587 2206 24 1.2905829502022286 2206 25 2.8061555180238784 2207 1 1.093171077369238 2207 2 -12.764869676530937 2207 3 -5.8894296390421434 2207 4 -4.5509793432482795 2207 5 0.7057800999938202 2207 6 1.2746455096639515 2207 7 2.4268561283060563 2207 8 2.6460265290279636 2207 9 0.027324554403136858 2207 10 -0.05900579641436465 2207 11 -2.065474931736482 2207 12 -2.7472829880393057 2207 13 -0.26539340072740103 2207 14 -5.223864674448343 2207 15 -1.8194632856196051 2207 16 -0.9221345961297835 2207 17 0.14549468826118428 2207 18 -0.012256529272267613 2207 19 -0.396500923468448 2207 20 -0.6746671111410905 2207 21 -3.72011368136123 2207 22 -4.62911362896924 2207 23 -1.7789179666511465 2207 24 -0.23878003066788978 2207 25 0.29804693350069655 2208 1 5.231555867973011 2208 2 6.616166319957211 2208 3 6.539156354816161 2208 4 8.213144736812247 2208 5 4.232569303879515 2208 6 6.533010232539141 2208 7 6.733227227540166 2208 8 6.821312473930721 2208 9 5.592617094144117 2208 10 5.0107327971453515 2208 11 6.98418217713499 2208 12 6.720910066746402 2208 13 8.926953350202147 2208 14 3.624992962010186 2208 15 5.069611535449891 2208 16 5.318928993485169 2208 17 6.244989353724868 2208 18 1.1526403201752622 2208 19 3.687917051713921 2208 20 4.80302251068742 2208 21 7.25193229639434 2208 22 6.467372495572057 2208 23 2.2544494872153287 2208 24 4.030146143557218 2208 25 5.329908498540771 2209 1 2.6221107486862856 2209 2 4.049742195615183 2209 3 4.577805173574905 2209 4 0.34080779140231243 2209 5 4.24503477667669 2209 6 4.755835670217788 2209 7 5.302411122729808 2209 8 3.972028675357109 2209 9 3.5654723986048893 2209 10 2.729770164699204 2209 11 -0.9516647766536792 2209 12 2.4016890886393547 2209 13 1.6028093642495556 2209 14 2.5670449903455435 2209 15 3.1940080861372273 2209 16 2.7126990633614128 2209 17 0.406702945315665 2209 18 2.4839074334405247 2209 19 2.965067159354607 2209 20 3.0600003130535107 2209 21 5.259416230470933 2209 22 3.9426600385180848 2209 23 -0.5818597001275698 2209 24 -3.6362928139517727 2209 25 -0.7089054895365692 2210 1 -1.9645266737137987 2210 2 -12.764869676530937 2210 3 -7.150625897175699 2210 4 -2.3343704960123937 2210 5 -0.924859020460449 2210 6 -1.3646199246721586 2210 7 -1.409984341843586 2210 8 -19.15673844793056 2210 9 -12.06365129951219 2210 10 -6.710697230508842 2210 11 -9.148019504476778 2210 12 -4.75899789509673 2210 13 -5.184269287435837 2210 14 -3.8286196753976185 2210 15 -5.429554070755696 2210 16 -4.834253376226091 2210 17 -4.294710527569258 2210 18 -4.3985947470530355 2210 19 -9.838129187677358 2210 20 -6.908359442731211 2210 21 -5.049228785177313 2210 22 -9.41128280689775 2210 23 -7.708902950858677 2210 24 -10.077665755309322 2210 25 -7.96904863252564 2211 1 0.025322662343402413 2211 2 4.235130323469063 2211 3 0.4343353728277073 2211 4 1.2463028100822626 2211 5 0.7642050755558927 2211 6 -0.448857667266644 2211 7 -18.999871982293023 2211 8 -12.66242026611238 2211 9 -9.141888020656214 2211 10 -7.317204721145547 2211 11 -9.232619010775723 2211 12 -4.890711803847154 2211 13 -6.535637605447413 2211 14 -5.006756379517469 2211 15 -5.2875298964281505 2211 16 -11.192697366692553 2211 17 -7.17530722487944 2211 18 -5.823401097070061 2211 19 -8.290780702828872 2211 20 -6.4653265860747595 2211 21 -4.3348837902845645 2211 22 -5.735806130016577 2211 23 -7.50198475201632 2211 24 -20.066429800253143 2211 25 -19.863243389079948 2212 1 -0.7635496675125317 2212 2 -12.764869676530937 2212 3 -7.195865198458931 2212 4 -18.100288099008647 2212 5 -18.145832803231986 2212 6 -19.035031909690886 2212 7 -9.151511326555317 2212 8 -8.76534500530761 2212 9 -4.8086012596030585 2212 10 -5.14643969382314 2212 11 -5.522935081317204 2212 12 -2.723160263857089 2212 13 -5.3369447341111 2212 14 -3.3766334286977973 2212 15 -3.4140202093938754 2212 16 -3.4814144556145585 2212 17 -3.6413405085356736 2212 18 -2.7980813007461656 2212 19 -3.298853231393206 2212 20 -3.415059571668401 2212 21 -1.4279817783620636 2212 22 -2.7791183505828982 2212 23 -2.9539066347783667 2212 24 -2.0582330789416687 2212 25 -2.3314810939979793 2213 1 -16.987934913414172 2213 2 -12.764869676530937 2213 3 -7.9025341131715265 2213 4 -5.175182613776579 2213 5 -18.145832803231986 2213 6 -9.6784918253027 2213 7 -18.999871982293023 2213 8 -9.715810177888367 2213 9 -7.650338826179805 2213 10 -6.50154016418352 2213 11 -5.46697603033844 2213 12 -4.6945452330761555 2213 13 -17.597190635750444 2213 14 -11.250690978673587 2213 15 -6.479369175292746 2213 16 -5.818608375514955 2213 17 -4.37287626975861 2213 18 -4.2318806712933075 2213 19 -5.182011044217443 2213 20 -5.141031419216301 2213 21 -3.2312085889036695 2213 22 -4.13593079438175 2213 23 -3.8290968540493076 2213 24 -3.0906914036286715 2213 25 -3.853749718193871 2214 1 -16.987934913414172 2214 2 -12.764869676530937 2214 3 -11.272320438611963 2214 4 -18.100288099008647 2214 5 -18.145832803231986 2214 6 -19.035031909690886 2214 7 -15.003499551821438 2214 8 -13.630740866310248 2214 9 -12.607276106472051 2214 10 -12.646079166933534 2214 11 -13.110759927195055 2214 12 -11.224199721109256 2214 13 -17.597190635750444 2214 14 -15.364600998622668 2214 15 -21.844348078246334 2214 16 -16.74231079236402 2214 17 -21.552201164273377 2214 18 -16.248203458654466 2214 19 -13.502982069579817 2214 20 -12.403916689039105 2214 21 -10.00439365617442 2214 22 -11.082774910933363 2214 23 -10.883746367567234 2214 24 -9.857239957447822 2214 25 -9.540389701050927 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 135766.0 3 9.0 4 701.0 5 2485.0 6 3105.0 7 3058.0 8 2531.0 9 2464.0 10 2373.0 11 2179.0 12 2124.0 13 2249.0 14 3241.0 15 6350.0 16 8783.0 17 9508.0 18 9736.0 19 11436.0 20 13924.0 21 16158.0 22 17281.0 23 18628.0 24 19195.0 25 19145.0 26 16151.0 27 13792.0 28 14611.0 29 16707.0 30 16095.0 31 18506.0 32 23134.0 33 28240.0 34 32834.0 35 36111.0 36 33634.0 37 32349.0 38 18402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81073839009865 18.938459417054567 13.139378444957087 26.1114237478897 2 13.190889025416979 20.294142186421524 42.31359491104183 24.20137387711966 3 18.914002432337725 25.22605624609211 29.054067981778083 26.805873339792086 4 11.064200559436376 17.37001331279075 38.91672774595007 32.649058381822805 5 11.877899393501616 37.66203387483416 36.345178234923154 14.114888496741077 6 29.95029576852569 37.94284958327821 19.643376863908678 12.463477784287415 7 26.481599804726763 30.840069781466273 24.493868994629985 18.18446141917698 8 24.693363973438977 38.03026885282881 20.795914339287478 16.48045283444474 9 26.455318516534575 15.227257509517036 20.570515887856573 37.74690808609182 10 14.200091142651457 29.515028630680728 35.56478723474517 20.720092991922648 11 33.1098157053347 21.87569258680851 25.64559828332964 19.36889342452715 12 24.219306940047378 24.521638154199227 32.278151588183654 18.980903317569737 13 28.466358725075736 21.53152945603385 25.101271314972028 24.900840503918385 14 19.79200084668124 23.07652873920564 28.449812638701932 28.681657775411185 15 21.926521720916593 32.660215324033345 23.636378869758286 21.776884085291776 16 20.856042609335628 28.10160790894719 29.503310203541055 21.539039278176126 17 20.48730974669742 28.83073464950849 29.92648647562195 20.755469128172145 18 20.440802299838086 27.110332749562172 32.468030267984005 19.980834682615736 19 22.512565967199688 26.668416985356767 27.680959835139795 23.13805721230375 20 23.18797425485339 27.98835774890302 28.58028670433983 20.24338129190376 21 21.54635136578938 26.044374799672354 26.69152849358697 25.717745340951296 22 22.258055446677684 29.095530825117876 28.123072241434343 20.523341486770097 23 21.045875664879503 28.471948903285654 29.101839044043317 21.38033638779153 24 22.409025629300427 27.45271762041198 29.295605078702746 20.842651671584843 25 21.891240368063293 27.847443841603436 29.008829207265883 21.25248658306739 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 522.0 1 522.0 2 1076.0 3 1630.0 4 1630.0 5 1630.0 6 2385.5 7 3141.0 8 3141.0 9 3141.0 10 3732.0 11 4323.0 12 4323.0 13 4323.0 14 5519.0 15 6715.0 16 6715.0 17 6715.0 18 9971.5 19 13228.0 20 13228.0 21 13228.0 22 19007.5 23 24787.0 24 24787.0 25 24787.0 26 33004.0 27 41221.0 28 41221.0 29 41221.0 30 50113.5 31 59006.0 32 59006.0 33 59006.0 34 65503.0 35 72000.0 36 72000.0 37 72000.0 38 74914.0 39 77828.0 40 77828.0 41 77828.0 42 77750.0 43 77672.0 44 77672.0 45 77672.0 46 75208.5 47 72745.0 48 72745.0 49 72745.0 50 66149.5 51 59554.0 52 59554.0 53 59554.0 54 50768.5 55 41983.0 56 41983.0 57 41983.0 58 34569.0 59 27155.0 60 27155.0 61 27155.0 62 21392.0 63 15629.0 64 15629.0 65 15629.0 66 11864.0 67 8099.0 68 8099.0 69 8099.0 70 5808.0 71 3517.0 72 3517.0 73 3517.0 74 2495.0 75 1473.0 76 1473.0 77 1473.0 78 999.0 79 525.0 80 525.0 81 525.0 82 352.0 83 179.0 84 179.0 85 179.0 86 111.0 87 43.0 88 43.0 89 43.0 90 28.0 91 13.0 92 13.0 93 13.0 94 8.5 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.453772053605658 2 35.47696147603162 3 5.030873008752111 4 12.752306299398853 5 12.82490069250157 6 13.681188264178337 7 9.107252098304228 8 7.430240050897642 9 0.10962569025848498 10 1.199194120669826 11 5.047023222049119 12 5.032993743831516 13 15.02736563919771 14 1.3509082455811223 15 2.6460248452271227 16 2.692681016974037 17 2.6039364105743115 18 1.2618373722461032 19 1.2057194593756881 20 0.28923563813734204 21 3.8068826010000083 22 1.360859387107562 23 0.10864688945260564 24 8.673317074364391 25 0.11566162856140752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 612995.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.50249631262083 #Duplication Level Percentage of deduplicated Percentage of total 1 97.50152813822784 91.16636275219545 2 1.780248995341773 3.3291545024498213 3 0.31441672451865676 0.8819624587479609 4 0.12748003736130736 0.47678806893233633 5 0.06727316098721156 0.3145104243572548 6 0.04779137404853088 0.2681167665448705 7 0.02841311309159211 0.18596879014536688 8 0.01646169313005223 0.12313675209537633 9 0.016792326405251783 0.14131119940086423 >10 0.08654848646002825 1.47361324706888 >50 0.008042062449151604 0.5352218346039108 >100 0.004289046839050132 0.7029837159048993 >500 7.14841139623565E-4 0.400869487553018 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 727 0.1185980309790455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.6313346764655504E-4 0.0 0.0 0.0 0.0 10 1.6313346764655504E-4 0.0 0.0 0.0 0.0 11 1.6313346764655504E-4 0.0 0.0 0.0 0.0 12 1.6313346764655504E-4 0.0 0.0 0.0 0.0 13 1.6313346764655504E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTATA 35 0.0060160705 19.003822 1 ATCCTAT 55 2.3961233E-4 16.930677 1 TATACTG 70 1.6094964E-7 16.316545 5 CTAGTAC 60 1.2775091E-4 14.527893 3 GTGTAGG 55 0.0046683284 14.512009 1 TGTAGGA 500 0.0 14.45888 2 CTATACT 45 0.005230486 14.266636 4 GTATAGA 95 5.22767E-6 14.002815 1 CTGTAGG 470 0.0 13.868746 1 CTTGGAC 55 0.0010918188 13.867536 3 AGATAGC 45 0.006393051 13.841284 6 TATTATA 60 0.007038105 13.640453 2 CATAATA 70 0.0012358952 13.640453 2 TAGGACC 230 0.0 13.491276 4 GTCCTAC 565 0.0 13.420397 1 GTTATAC 50 0.00931535 13.075105 3 ACAGCGC 45 0.0097579565 12.983911 8 GGTCTCG 45 0.0097579565 12.983911 8 GGACCTG 410 0.0 12.912905 6 GTAGGAC 515 0.0 12.905848 3 >>END_MODULE