FastQCFastQC Report
Thu 26 May 2016
SRR1926681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612995
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16430.26802828734328993No Hit
CTGTAGGACGTGGAATATGGCAAGA14870.24257946639042732No Hit
GTCCTAAAGTGTGTATTTCTCATTT14550.23735919542573758No Hit
CTTTAGGACGTGAAATATGGCGAGG14300.23328085873457366No Hit
GTCCTACAGTGTGCATTTCTCATTT13430.21908824704932342No Hit
CTGTAGGACCTGGAATATGGCGAGA10810.176347278525926No Hit
CTGAAGGACCTGGAATATGGCGAGA7780.1269178378290198No Hit
GTATCAACGCAGAGTACTTTTTTTT6750.11011509066142465No Hit
CCCATGTACTCTGCGTTGATACCAC6380.10407915235850211No Hit
GTACATGGGAAGCAGTGGTATCAAC6290.1026109511496831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGA859.604264E-1015.7079052
CGCCACC500.00151875213.27543114
TAGGACC4150.013.0946344
TGAAGTG905.1597453E-712.711755
GGCGAGG3700.012.54964419
ACGAAAT854.014435E-612.27140612
TCCTACT550.002976952112.1379272
CTAAACC550.00298428512.1339434
GTATAGG550.002989794612.1309561
CCAACGA550.003122762312.06069819
CTAGGAC959.91804E-712.0436993
AGGACCT6200.011.840385
CGTCAAG657.7705225E-411.7348853
GATTAAA1302.4683686E-911.7310341
GTCCTAC8200.011.5075011
AGGACGT7800.011.4894655
TAGGACG8400.011.4632744
GGACGTG7750.011.4405756
GTATAGA1104.7415597E-711.2644591
CGTTGAT1354.838512E-911.2384614
Produced by FastQC (version 0.11.5)