FastQCFastQC Report
Thu 26 May 2016
SRR1926680_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926680_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715239
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8340.11660437979472596No Hit
GTCCTAAAGTGTGTATTTCTCATTT7770.10863501570803605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTTA450.001184437117.823191
GTATAAT552.3100185E-417.0130461
GCAGGAC852.562956E-916.7693653
TGTAGGA6050.015.630542
TAGGACC3100.015.6118894
GTCCTAA3000.015.1497131
TAGCACC505.8580993E-415.0567984
GTCTTAC902.8404356E-614.8526591
CTGTAGG5950.014.8276981
GTAGGAC6750.014.7818853
TAGGACA2350.014.1872574
CTTAGGT550.001041585313.9549273
CAGGAGT807.982608E-613.4435714
GTCATAA600.00804850613.36739351
GTCCTAT600.00804850613.36739351
CTATATA802.5888206E-413.3673931
TCCTACA7550.012.8881832
TCCTAAA3300.012.8744312
GGACCTA653.6538506E-412.83478456
CCAGAAC600.002006869812.7920173
Produced by FastQC (version 0.11.5)