##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926680_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 715239 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.660665875322795 26.0 2.0 30.0 2.0 31.0 2 15.425161379622756 12.0 2.0 30.0 2.0 31.0 3 18.74520405067397 16.0 7.0 30.0 2.0 31.0 4 20.795973094308337 27.0 2.0 35.0 2.0 35.0 5 20.92134377459842 27.0 2.0 35.0 2.0 35.0 6 21.87229163957782 29.0 2.0 35.0 2.0 36.0 7 21.761225268756316 29.0 2.0 35.0 2.0 37.0 8 21.85791462713862 29.0 2.0 35.0 2.0 37.0 9 24.47696923685649 31.0 10.0 37.0 2.0 39.0 10 25.130378796458245 31.0 8.0 37.0 2.0 39.0 11 22.387427139739305 28.0 2.0 35.0 2.0 39.0 12 23.440635927291435 30.0 2.0 37.0 2.0 39.0 13 20.2579445472073 25.0 2.0 35.0 2.0 39.0 14 23.394350699556373 29.0 2.0 37.0 2.0 40.0 15 24.43655197773052 30.0 2.0 37.0 2.0 40.0 16 24.77726606071537 31.0 2.0 38.0 2.0 40.0 17 24.105391344711347 30.0 2.0 37.0 2.0 40.0 18 24.17014871951893 30.0 2.0 37.0 2.0 40.0 19 24.518154071576074 30.0 2.0 38.0 2.0 40.0 20 24.767400826856477 31.0 2.0 38.0 2.0 40.0 21 22.702896514312 30.0 2.0 36.0 2.0 40.0 22 23.572535893596406 30.0 2.0 37.0 2.0 40.0 23 23.52703921346571 30.0 2.0 37.0 2.0 40.0 24 22.659102761454562 30.0 2.0 38.0 2.0 40.0 25 22.44884577043478 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.039394996920603 1101 2 9.548707413370494 1101 3 6.372739896772632 1101 4 8.135627145587527 1101 5 7.382668704436959 1101 6 7.157941002433343 1101 7 6.871649174025503 1101 8 5.3806116455711575 1101 9 5.4829527452964015 1101 10 5.077866688468397 1101 11 7.475131440142455 1101 12 5.1118118045552485 1101 13 9.539172892912333 1101 14 6.824957566593952 1101 15 6.044920510223932 1101 16 5.882875584391307 1101 17 1.8618044920756311 1101 18 3.986068651948049 1101 19 4.833895159853718 1101 20 2.7065913012910343 1101 21 0.11540924225075955 1101 22 3.568323048536822 1101 23 4.9007788166986685 1101 24 6.278527001024326 1101 25 6.72408634533911 1102 1 4.834742160473908 1102 2 9.681380797883534 1102 3 6.095420586841566 1102 4 7.6874625814788935 1102 5 7.219003165573987 1102 6 6.384796878916394 1102 7 6.505099369044395 1102 8 6.446173227870219 1102 9 5.7350311555421385 1102 10 4.935549834129208 1102 11 7.688983563175089 1102 12 5.332413064049412 1102 13 9.3972376416325 1102 14 7.056902844307107 1102 15 5.030366536858601 1102 16 5.481643435047786 1102 17 6.406727538859759 1102 18 6.498703726506893 1102 19 5.262408186734625 1102 20 5.826781300161869 1102 21 4.446831159190534 1102 22 5.7230566270212115 1102 23 6.10382565524316 1102 24 7.310525880022826 1102 25 6.995894518038643 1103 1 3.7990665610419434 1103 2 8.246225854187479 1103 3 5.164039096276223 1103 4 6.993094482529653 1103 5 6.0316526897669505 1103 6 5.728080461005945 1103 7 6.10632664855655 1103 8 5.4460082120465 1103 9 4.522846825335204 1103 10 4.077353888451597 1103 11 4.771596236116466 1103 12 3.7275369491655326 1103 13 7.791641208713145 1103 14 6.194061805898819 1103 15 5.26893011861949 1103 16 4.896018709788862 1103 17 2.1478629025039666 1103 18 1.1324767825040887 1103 19 3.4803598542161005 1103 20 3.748361809396595 1103 21 6.086924115822185 1103 22 1.6318350580151524 1103 23 3.6724866168814323 1103 24 4.828531776770852 1103 25 0.730718197603931 1104 1 4.982376036732386 1104 2 9.438116678383235 1104 3 6.474399774035181 1104 4 6.825608445475943 1104 5 6.708732595036295 1104 6 6.309987551470492 1104 7 6.322183467703503 1104 8 6.271243070182699 1104 9 5.311549541606926 1104 10 4.162317381883511 1104 11 7.215947358704369 1104 12 4.201752187503047 1104 13 8.422841083267034 1104 14 6.2161676449980305 1104 15 5.587483154260589 1104 16 4.98237328214482 1104 17 5.6063711301940415 1104 18 3.0819934717497084 1104 19 4.124140681554739 1104 20 3.232602641701927 1104 21 2.2779972521162897 1104 22 4.5643189381225895 1104 23 5.03725954528764 1104 24 5.624039809965613 1104 25 4.9644847372765994 1105 1 2.720008392395311 1105 2 8.443825546253237 1105 3 5.735540703390296 1105 4 4.896369573742014 1105 5 1.7080565705279689 1105 6 4.240431058615506 1105 7 5.134298658123566 1105 8 2.3169633674776264 1105 9 3.4404007220522175 1105 10 3.045432030055288 1105 11 5.985422351966854 1105 12 3.59715509096662 1105 13 4.191104297727854 1105 14 3.3246121067597834 1105 15 3.8144501030911826 1105 16 4.115844937495709 1105 17 4.773457633406942 1105 18 1.7571036260794166 1105 19 1.7467163933773726 1105 20 0.9339790431864543 1105 21 2.033520554181056 1105 22 3.4172404225191535 1105 23 0.8669766994907135 1105 24 3.779522199884344 1105 25 4.43951241791509 1106 1 5.80533892271988 1106 2 9.984207083456468 1106 3 7.008327982501598 1106 4 7.993786481891291 1106 5 7.577762030963694 1106 6 6.851576852345158 1106 7 6.586821206263004 1106 8 6.5011542702658325 1106 9 5.576704903881435 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23 -0.23888402626180394 1107 24 3.1772819960219856 1107 25 3.9534832648376614 1108 1 5.101440583253627 1108 2 9.988350676622225 1108 3 0.6777865348791536 1108 4 5.729633523459299 1108 5 7.063521707615553 1108 6 7.201681721841247 1108 7 7.283418176896387 1108 8 7.001793471804213 1108 9 5.3773985149625325 1108 10 4.427851917094994 1108 11 6.743163020498404 1108 12 2.166169812223437 1108 13 3.0042086965235306 1108 14 4.438505469479118 1108 15 4.636155558652703 1108 16 4.5235556235144365 1108 17 -0.2671195378136133 1108 18 3.2647317424555204 1108 19 4.379852878171061 1108 20 4.6469770119104545 1108 21 4.427542260892427 1108 22 1.3122501393649308 1108 23 4.0659770730948 1108 24 4.994564316681142 1108 25 5.1868957765216415 1109 1 2.9134074144017603 1109 2 -12.913146565300547 1109 3 -5.363639198299982 1109 4 1.6082263898722076 1109 5 0.42894690829619364 1109 6 1.805742590709709 1109 7 2.595845265927739 1109 8 3.4052456267511815 1109 9 2.880735778942352 1109 10 2.2925013986976133 1109 11 0.6245944067421476 1109 12 1.1971506252426458 1109 13 2.481909691046493 1109 14 2.831006704509697 1109 15 2.643772684662416 1109 16 2.684968283981661 1109 17 3.1303691387068007 1109 18 3.2657916920700565 1109 19 1.8871315143187495 1109 20 1.0339008930169165 1109 21 0.25620834584865193 1109 22 2.2110981381737496 1109 23 2.6229421092687346 1109 24 3.543371526357472 1109 25 3.862903880088812 1110 1 3.247477752586338 1110 2 8.669984010530758 1110 3 5.748075802248714 1110 4 7.130000500158005 1110 5 8.048232929164794 1110 6 3.766198871387129 1110 7 6.380091803465959 1110 8 7.122832510532334 1110 9 5.4687227946701 1110 10 5.093143859137985 1110 11 2.8260778968594664 1110 12 5.822983327089442 1110 13 9.73459586269265 1110 14 7.463664655688159 1110 15 6.8409607676511115 1110 16 6.304324433946238 1110 17 6.766322508052724 1110 18 6.7524713845669595 1110 19 5.811372610358596 1110 20 6.092623621402382 1110 21 3.916599107768814 1110 22 6.185234787314634 1110 23 6.412585989823302 1110 24 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2207 7 2.9962630654167164 2207 8 3.0379316611060574 2207 9 1.0780828634783362 2207 10 0.4260355359010788 2207 11 -1.2252033382404264 2207 12 -2.1157610286921127 2207 13 0.6676523980493805 2207 14 -5.0245866827103 2207 15 -1.5537203874731347 2207 16 -0.5504507953537612 2207 17 0.6678483452936561 2207 18 0.7995190828133971 2207 19 0.25177384142655157 2207 20 0.05222331874163544 2207 21 -3.4027855704618304 2207 22 -3.9426798310536384 2207 23 -1.1272416845365463 2207 24 0.4199596324924215 2207 25 0.9808193080976153 2208 1 4.729275975001574 2208 2 6.348485776423328 2208 3 5.984083678704902 2208 4 7.8045677497640895 2208 5 3.764762166147019 2208 6 6.123961467870934 2208 7 6.3607904028152795 2208 8 6.462041635640674 2208 9 4.6937956852961165 2208 10 4.4551676109962095 2208 11 6.3357592195993 2208 12 5.96963314214862 2208 13 8.249317820448567 2208 14 2.5916774836959746 2208 15 4.341511043160047 2208 16 4.898102330501867 2208 17 5.875692781297456 2208 18 1.065052000392992 2208 19 3.281270776911125 2208 20 4.310163398062905 2208 21 6.44774952692471 2208 22 5.883349745714547 2208 23 2.122602859989833 2208 24 2.969875964080039 2208 25 4.416413965044651 2209 1 3.2041839204987212 2209 2 4.99492697586652 2209 3 5.482209663043289 2209 4 0.8090309983871578 2209 5 5.052789867555685 2209 6 5.451501723995552 2209 7 5.968549504458451 2209 8 4.784463789450804 2209 9 4.603458801485086 2209 10 3.785687634336803 2209 11 0.09377614701846326 2209 12 3.437493179061878 2209 13 2.818983826773941 2209 14 4.024045548054556 2209 15 4.502872114486053 2209 16 4.122151923779189 2209 17 1.941207417643298 2209 18 3.60221494738094 2209 19 4.294746495192335 2209 20 4.346610213150882 2209 21 6.39836843683111 2209 22 5.119360479461648 2209 23 0.7391860236648427 2209 24 -2.7016673504674067 2209 25 -0.20566960379821708 2210 1 -2.6263967659854757 2210 2 -12.913146565300547 2210 3 -7.4417742023286415 2210 4 -2.5730696803703133 2210 5 -1.5210340621717542 2210 6 -2.000854356164165 2210 7 -2.1409285728571206 2210 8 -19.393793604633927 2210 9 -12.62437610070445 2210 10 -7.713148908511588 2210 11 -9.537362185558719 2210 12 -5.096562882924779 2210 13 -5.586008554519752 2210 14 -4.612385847369644 2210 15 -6.285241432831899 2210 16 -5.623755642695453 2210 17 -5.036074349947398 2210 18 -5.1472490744064 2210 19 -10.218855341938912 2210 20 -7.321109347289795 2210 21 -5.34173264605457 2210 22 -9.472079246341178 2210 23 -7.88747512754138 2210 24 -10.378684401373583 2210 25 -8.214230005079736 2211 1 -0.774436409044096 2211 2 3.480401821796228 2211 3 -0.34646675066445454 2211 4 0.37360579016663564 2211 5 0.10481469930319065 2211 6 -1.0001453454456666 2211 7 -19.277653533111494 2211 8 -12.971212959472638 2211 9 -9.530207168964361 2211 10 -8.017088854149826 2211 11 -9.8062593084881 2211 12 -5.887936799535886 2211 13 -6.973379701758072 2211 14 -5.699809561406262 2211 15 -6.270985573557063 2211 16 -11.982471474276558 2211 17 -8.377384160706924 2211 18 -6.890978240004635 2211 19 -9.410181820219849 2211 20 -7.583354847059017 2211 21 -5.432332163978565 2211 22 -6.793388031407076 2211 23 -8.496328145644872 2211 24 -20.228445927605698 2211 25 -20.029810291248257 2212 1 -0.924368160116579 2212 2 -12.913146565300547 2212 3 -7.355988172470683 2212 4 -18.28849098402691 2212 5 -18.357282074890357 2212 6 -19.271919538994055 2212 7 -9.403732980434464 2212 8 -8.888612257483668 2212 9 -5.126825364964137 2212 10 -5.273912354066255 2212 11 -5.156976618644656 2212 12 -2.949583687941928 2212 13 -5.553866079829273 2212 14 -3.632177659907569 2212 15 -3.670017553054528 2212 16 -3.715905091748283 2212 17 -3.764553362333757 2212 18 -2.9646745462055364 2212 19 -3.5011659976113485 2212 20 -3.556402092030879 2212 21 -1.489321411290394 2212 22 -2.720893742031624 2212 23 -2.6932597295771252 2212 24 -1.8018483455677021 2212 25 -2.1282558871031796 2213 1 -17.20588802194732 2213 2 -12.913146565300547 2213 3 -7.909510353483453 2213 4 -5.124192582605952 2213 5 -18.357282074890357 2213 6 -9.826981705956754 2213 7 -19.277653533111494 2213 8 -9.778340673905685 2213 9 -7.614628185408524 2213 10 -6.557874105968999 2213 11 -5.4404153275106175 2213 12 -4.74566899457399 2213 13 -17.67821841143549 2213 14 -11.27399594770088 2213 15 -6.309249768824351 2213 16 -5.673716532432749 2213 17 -4.17074919823629 2213 18 -4.17179958876989 2213 19 -5.008490133976796 2213 20 -5.117751512388757 2213 21 -3.304892755280921 2213 22 -4.128178611823053 2213 23 -3.942688370248092 2213 24 -3.0463855368419317 2213 25 -3.893931072775789 2214 1 -17.20588802194732 2214 2 -12.913146565300547 2214 3 -11.423000262280699 2214 4 -18.28849098402691 2214 5 -18.357282074890357 2214 6 -19.271919538994055 2214 7 -15.342938507204757 2214 8 -13.88084023675828 2214 9 -13.028034345965866 2214 10 -12.85318696546522 2214 11 -13.325024977884723 2214 12 -11.36858196082621 2214 13 -17.67821841143549 2214 14 -15.369828782532785 2214 15 -21.989533960653837 2214 16 -16.822986266186973 2214 17 -21.690287386513376 2214 18 -16.33928594516927 2214 19 -13.593221265314284 2214 20 -12.365383093757995 2214 21 -10.087421584001964 2214 22 -11.100341065002263 2214 23 -10.996541249438955 2214 24 -9.945544373201553 2214 25 -9.71167557622235 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 159627.0 3 9.0 4 840.0 5 2938.0 6 3659.0 7 3652.0 8 3080.0 9 2974.0 10 2816.0 11 2613.0 12 2378.0 13 2560.0 14 3555.0 15 7485.0 16 10412.0 17 11311.0 18 11717.0 19 13786.0 20 16518.0 21 19009.0 22 19677.0 23 21437.0 24 22153.0 25 22367.0 26 18505.0 27 15559.0 28 16522.0 29 18943.0 30 18305.0 31 21103.0 32 26500.0 33 32260.0 34 37719.0 35 41777.0 36 39507.0 37 38759.0 38 23207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.883844961340145 19.016495837807295 12.994637685592261 26.1050215152603 2 13.058552040769275 20.498154535933306 42.373216289266885 24.070077134030534 3 18.724754162881332 25.571647130889215 29.23456841270452 26.46903029352493 4 11.034855426988688 17.38613419274676 39.23274054515804 32.346269835106526 5 11.594200557849872 38.01939069356535 36.39703922876528 13.989369519819498 6 29.504786161380718 38.45232171385171 19.618514784066207 12.424377340701358 7 26.108635901361303 31.276380017315326 24.50303326795076 18.111950813372616 8 24.429030108438845 38.42430484037075 20.973223480947475 16.17344157024293 9 26.388816959880412 15.382278962934553 20.63325210409846 37.595651973086575 10 13.893505388542257 29.980998298355075 35.72646057855927 20.399035734543393 11 32.89451486783652 22.057435102122962 25.845352006795373 19.202698023245148 12 23.907114355000324 24.869262832105015 32.56808115783919 18.655541655055465 13 28.38996306033479 21.883423741222668 25.254251389397318 24.472361809045225 14 19.600121562590534 23.37963883413523 28.53326781829233 28.486971784981908 15 21.60206539853039 33.22468116705378 23.664010085223595 21.509243349192232 16 20.56228302645402 28.4375310734219 29.82400013834428 21.176185761779802 17 20.135634965278427 29.08613280659933 30.135332850909435 20.64289937721281 18 20.116151862956126 27.540875211240028 32.656750387007335 19.68622253879651 19 22.384804324765863 26.8242978778923 27.83798578454452 22.952912012797313 20 22.79634448644465 28.410569470460896 28.898634930961382 19.894451112133073 21 21.329489130545802 26.295047427560075 27.152843537931105 25.22261990396302 22 21.90462390383121 29.419586350327947 28.431702629100382 20.244087116740467 23 20.883052387144456 28.91181933594707 29.21527761253361 20.989850664374863 24 21.917521843123026 27.84329519113117 29.560309154743386 20.678873811002415 25 21.65351354831846 28.08039126211146 29.37881260795682 20.887282581613256 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 719.0 1 719.0 2 1561.5 3 2404.0 4 2404.0 5 2404.0 6 3523.0 7 4642.0 8 4642.0 9 4642.0 10 5328.0 11 6014.0 12 6014.0 13 6014.0 14 7583.0 15 9152.0 16 9152.0 17 9152.0 18 13282.0 19 17412.0 20 17412.0 21 17412.0 22 24353.0 23 31294.0 24 31294.0 25 31294.0 26 41095.5 27 50897.0 28 50897.0 29 50897.0 30 61306.0 31 71715.0 32 71715.0 33 71715.0 34 78358.0 35 85001.0 36 85001.0 37 85001.0 38 87505.0 39 90009.0 40 90009.0 41 90009.0 42 89180.5 43 88352.0 44 88352.0 45 88352.0 46 85470.0 47 82588.0 48 82588.0 49 82588.0 50 74761.5 51 66935.0 52 66935.0 53 66935.0 54 56602.5 55 46270.0 56 46270.0 57 46270.0 58 37970.0 59 29670.0 60 29670.0 61 29670.0 62 23342.5 63 17015.0 64 17015.0 65 17015.0 66 12922.0 67 8829.0 68 8829.0 69 8829.0 70 6393.0 71 3957.0 72 3957.0 73 3957.0 74 2725.0 75 1493.0 76 1493.0 77 1493.0 78 1046.5 79 600.0 80 600.0 81 600.0 82 385.0 83 170.0 84 170.0 85 170.0 86 120.5 87 71.0 88 71.0 89 71.0 90 44.0 91 17.0 92 17.0 93 17.0 94 12.5 95 8.0 96 8.0 97 8.0 98 6.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.1497303698484 2 36.323242999892344 3 5.506550957092664 4 13.58189360479504 5 13.432712701628407 6 14.584076092047555 7 9.403569995484027 8 7.684004927024393 9 0.11185072402371794 10 1.4035867730926306 11 5.520532297595629 12 5.676843684418775 13 15.974939845282485 14 1.5484334607033454 15 2.8467407398086517 16 2.9805421684220237 17 2.81556235048704 18 1.4641259774704678 19 1.4108570701541723 20 0.28172401113473955 21 4.177344915475806 22 1.5812896108853125 23 0.11296923126395512 24 9.044668984772922 25 0.11646456638969632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 715239.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.88786762137896 #Duplication Level Percentage of deduplicated Percentage of total 1 97.3740827638108 90.44870909518058 2 1.8517771486629717 3.4401526129860147 3 0.338708990815443 0.9438586770310721 4 0.1399054021969725 0.5198205791515266 5 0.07583560997873907 0.352210405034582 6 0.04306382316001607 0.24000640229748277 7 0.02714620765896684 0.17650873404140027 8 0.024279030857994696 0.1804181923450229 9 0.01874749828817572 0.15672736253016856 >10 0.09193366566241569 1.6110955343802464 >50 0.008186945084820766 0.5387769581231423 >100 0.0055606072631738 0.9107845018918106 >500 7.72306559458111E-4 0.4809309450068855 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 834 0.11660437979472596 No Hit GTCCTAAAGTGTGTATTTCTCATTT 777 0.10863501570803605 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.3981340502964743E-4 0.0 12 0.0 0.0 0.0 1.3981340502964743E-4 0.0 13 0.0 0.0 0.0 1.3981340502964743E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCTTA 45 0.0011844371 17.82319 1 GTATAAT 55 2.3100185E-4 17.013046 1 GCAGGAC 85 2.562956E-9 16.769365 3 TGTAGGA 605 0.0 15.63054 2 TAGGACC 310 0.0 15.611889 4 GTCCTAA 300 0.0 15.149713 1 TAGCACC 50 5.8580993E-4 15.056798 4 GTCTTAC 90 2.8404356E-6 14.852659 1 CTGTAGG 595 0.0 14.827698 1 GTAGGAC 675 0.0 14.781885 3 TAGGACA 235 0.0 14.187257 4 CTTAGGT 55 0.0010415853 13.954927 3 CAGGAGT 80 7.982608E-6 13.443571 4 GTCATAA 60 0.008048506 13.3673935 1 GTCCTAT 60 0.008048506 13.3673935 1 CTATATA 80 2.5888206E-4 13.367393 1 TCCTACA 755 0.0 12.888183 2 TCCTAAA 330 0.0 12.874431 2 GGACCTA 65 3.6538506E-4 12.8347845 6 CCAGAAC 60 0.0020068698 12.792017 3 >>END_MODULE