FastQCFastQC Report
Thu 26 May 2016
SRR1926680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715239
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18420.2575362920646106No Hit
GTCCTACAGTGGACATTTCTAAATT18200.25446039715395835No Hit
CTTTAGGACGTGAAATATGGCGAGG17820.24914748776283172No Hit
GTCCTAAAGTGTGTATTTCTCATTT17750.2481687939276242No Hit
GTCCTACAGTGTGCATTTCTCATTT13230.18497313485422356No Hit
GTATCAACGCAGAGTACTTTTTTTT12460.17420750266694068No Hit
CTGTAGGACCTGGAATATGGCGAGA11130.15561231979799758No Hit
CTGAAGGACCTGGAATATGGCGAGA9890.1382754575743213No Hit
CCCATGTACTCTGCGTTGATACCAC8640.12079878194561537No Hit
GTACATGGGAAGCAGTGGTATCAAC8170.11422755190922196No Hit
GGTATCAACGCAGAGTACTTTTTTT7690.10751650846779887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACT500.001466019313.3383244
TAGGACC4550.013.1917484
TAGGACT1051.9083018E-812.7031664
TGTAGGA11750.012.3291052
CTGTAGG11200.012.2477591
GTAGGAC11200.012.1678353
GACGTGG3950.012.0261597
GGACCTG7800.011.9702896
AGGACCT8050.011.8352475
GTCCTAG1156.785922E-811.5969111
TTAGACT1001.8590763E-611.4328494
CGTGGAA4150.011.2215859
CTTCTAG855.167155E-511.2070991
ACGTGGA4350.011.1426168
CTAGGAC1201.2164128E-711.1183963
AAGGTGC600.005745957611.115276
GTCTAGT600.005751974411.1137081
TCAGCAC951.3105186E-511.0347993
ATGGCAA8500.010.927670517
TCTAGGA1407.90169E-910.8922552
Produced by FastQC (version 0.11.5)