FastQCFastQC Report
Thu 26 May 2016
SRR1926679_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926679_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686621
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9460.13777615307425786No Hit
GTCCTAAAGTGTGTATTTCTCATTT7800.11359978794706249No Hit
CTGTAGGACGTGGAATATGGCAAGA6890.10034647935323854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCG250.003519667820.850785
CCATTGG450.001206022717.7750191
GATTAAA552.3579694E-416.9670641
CCCCTAC500.00244056215.9975171
TTAGACT556.874881E-515.6239164
GATATAC1456.730261E-1114.7103611
GTCCTAA4200.014.6009081
TGTAGGA7300.014.2493082
ATTTAGA1900.014.032911
TCCTGCG600.0068834813.6868342
TCCTAAA4750.013.5427632
ACTGTTC1450.013.4648548
TTTAGAA2450.013.4075122
GTATAAG600.00819305813.33126451
GTCTTAG600.00819305813.33126451
CTGTAGG7200.013.3312641
TAGGACA2450.013.1527854
CTAGGAC755.5002616E-513.1376343
GTCCTAC6300.012.908051
TAGGACC3000.012.88972954
Produced by FastQC (version 0.11.5)