##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926679_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 686621 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.67189905348074 26.0 2.0 30.0 2.0 31.0 2 15.423572829843538 12.0 2.0 30.0 2.0 31.0 3 18.7697099273107 16.0 7.0 30.0 2.0 31.0 4 20.815345001099587 27.0 2.0 35.0 2.0 35.0 5 20.960458535349197 27.0 2.0 35.0 2.0 35.0 6 21.864677893626908 29.0 2.0 35.0 2.0 36.0 7 21.7462734172127 29.0 2.0 35.0 2.0 37.0 8 21.878420555153426 29.0 2.0 35.0 2.0 37.0 9 24.513839512627783 31.0 10.0 37.0 2.0 39.0 10 25.188422725200656 31.0 10.0 37.0 2.0 39.0 11 22.425610343988897 28.0 2.0 35.0 2.0 39.0 12 23.46584797144276 30.0 2.0 37.0 2.0 39.0 13 20.260101278580176 25.0 2.0 35.0 2.0 39.0 14 23.422519847193723 29.0 2.0 37.0 2.0 40.0 15 24.466766964599103 30.0 2.0 37.0 2.0 40.0 16 24.834192371046036 31.0 2.0 38.0 2.0 40.0 17 24.151585809347516 30.0 2.0 37.0 2.0 40.0 18 24.216205155391403 30.0 2.0 37.0 2.0 40.0 19 24.562643729218884 30.0 2.0 38.0 2.0 40.0 20 24.797984623249217 31.0 2.0 38.0 2.0 40.0 21 22.731016091846886 30.0 2.0 36.0 2.0 40.0 22 23.608258413302245 30.0 2.0 37.0 2.0 40.0 23 23.556257382165708 30.0 2.0 37.0 2.0 40.0 24 22.694630662330457 30.0 2.0 38.0 2.0 40.0 25 22.48438075736105 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.3780717680048795 1101 2 8.92293952804968 1101 3 5.6686328171871025 1101 4 7.371750768864448 1101 5 6.681444289113845 1101 6 6.157068317247084 1101 7 5.784424439120766 1101 8 4.539851846120456 1101 9 4.436917549429239 1101 10 4.220820534119561 1101 11 6.814696995470076 1101 12 4.582607087586862 1101 13 8.769170583566403 1101 14 6.136577180542943 1101 15 5.288912332834606 1101 16 4.89169519597127 1101 17 1.065171951626752 1101 18 3.2297736612566688 1101 19 4.002573838080334 1101 20 2.040575607814489 1101 21 -0.6735980334770879 1101 22 3.0619080865761923 1101 23 4.3787112557694705 1101 24 5.673034214118022 1101 25 6.015651492801954 1102 1 4.549597769709909 1102 2 9.405197253274158 1102 3 5.974594885809015 1102 4 7.155433411882129 1102 5 6.788655236734002 1102 6 5.907141943535542 1102 7 5.9808173045673385 1102 8 5.637877775859941 1102 9 4.871611585146294 1102 10 4.5785047935940995 1102 11 7.169383059619054 1102 12 4.759006817439278 1102 13 9.037285418433154 1102 14 6.585272389851962 1102 15 4.42551179475376 1102 16 4.754276434000076 1102 17 5.845200959277243 1102 18 5.902419003978736 1102 19 4.7778044046152495 1102 20 5.125273518599357 1102 21 3.9520017716623563 1102 22 5.126473359325608 1102 23 5.363197698567934 1102 24 6.530619936153872 1102 25 6.378061120684727 1103 1 3.960088463449331 1103 2 8.21777553793366 1103 3 5.657894158448389 1103 4 7.1711782423477715 1103 5 6.299537079087923 1103 6 6.0862836140477725 1103 7 6.311990530664424 1103 8 5.813870480279039 1103 9 4.912352570049716 1103 10 4.180581397193546 1103 11 4.773384641793225 1103 12 4.01545747338232 1103 13 7.666940196818899 1103 14 6.091212135385618 1103 15 5.324118602497624 1103 16 4.861119337289679 1103 17 2.8223912816452312 1103 18 1.6320979544698702 1103 19 3.6297348768502893 1103 20 4.319836992350648 1103 21 6.203488356143865 1103 22 2.002444089103445 1103 23 4.212009969207617 1103 24 5.557758618114889 1103 25 1.87665694110796 1104 1 4.614899248663264 1104 2 9.171944442376295 1104 3 5.929695871285428 1104 4 6.7349548574445315 1104 5 6.662712237753457 1104 6 6.277723182546815 1104 7 6.177713866480968 1104 8 6.12082548798854 1104 9 5.219723370971391 1104 10 4.1363080824554075 1104 11 6.999550162141105 1104 12 3.8979626852347344 1104 13 8.133015513139359 1104 14 5.76291596357062 1104 15 5.515247769813968 1104 16 5.0326831240108625 1104 17 5.2748768010691265 1104 18 3.2929565097039557 1104 19 3.7783318635044836 1104 20 2.8976018728389725 1104 21 2.311639346828944 1104 22 4.276288674840583 1104 23 4.683796667289769 1104 24 5.442588229521483 1104 25 4.606976062398303 1105 1 2.4334454205300347 1105 2 8.326878018991623 1105 3 5.6601979677423735 1105 4 5.028502239079398 1105 5 1.454424330026029 1105 6 3.9172058857095777 1105 7 4.8725683669606035 1105 8 2.1374925341902937 1105 9 2.8483779380594356 1105 10 2.613867595929378 1105 11 6.062532112401136 1105 12 3.4112011063308145 1105 13 4.267016543577125 1105 14 3.281868759842826 1105 15 3.4683173072840425 1105 16 3.6530095052102105 1105 17 3.9824979130879576 1105 18 1.9617938798673755 1105 19 1.5189220026135786 1105 20 0.5065436246401056 1105 21 1.5206025898044615 1105 22 3.206524195339746 1105 23 0.8477999464729358 1105 24 3.553059270519814 1105 25 4.191763015120568 1106 1 6.367386630890291 1106 2 10.803228150058173 1106 3 7.462500662492847 1106 4 8.962560615518061 1106 5 8.594676978915341 1106 6 7.537596111146819 1106 7 7.152623719266522 1106 8 6.874820052203713 1106 9 6.092401601447531 1106 10 5.504659104785819 1106 11 7.7819145015769315 1106 12 5.305048639291574 1106 13 4.636346208017635 1106 14 5.58040792763089 1106 15 5.758609785707112 1106 16 5.867476118770835 1106 17 6.238719327857957 1106 18 6.202750426504672 1106 19 5.976262804071155 1106 20 5.4503482775893275 1106 21 5.716305298559401 1106 22 6.154492057169744 1106 23 6.550944781078108 1106 24 7.46146382210998 1106 25 6.257570140343372 1107 1 3.759597769709906 1107 2 -12.910820062743158 1107 3 -5.084766586052455 1107 4 1.6098922863410046 1107 5 3.965408483487245 1107 6 4.787066185959787 1107 7 4.371715572965609 1107 8 5.077910243392406 1107 9 3.474328035362742 1107 10 2.940247217836525 1107 11 5.379285657021651 1107 12 3.2150133109457713 1107 13 4.4820797907275285 1107 14 4.0125234721030445 1107 15 3.3987801930221586 1107 16 3.4255102002338447 1107 17 4.045255071831356 1107 18 -1.5555247189649855 1107 19 1.3775013743122173 1107 20 1.8066263324521685 1107 21 -1.3076410854805012 1107 22 1.7895253073775557 1107 23 -1.2088910460208098 1107 24 2.0565614945954316 1107 25 2.7015892592128665 1108 1 5.633627253739391 1108 2 10.549458397535302 1108 3 0.848515559729691 1108 4 6.352578609027326 1108 5 7.714851562930328 1108 6 7.840475276868876 1108 7 7.91227863602867 1108 8 7.760774698756862 1108 9 6.332475046009755 1108 10 5.4962771113664175 1108 11 7.660643150879146 1108 12 3.292152950585411 1108 13 3.2389000200477582 1108 14 5.2190386236181965 1108 15 5.4889726582146245 1108 16 5.540821420545065 1108 17 1.2919074059836895 1108 18 4.629925731485464 1108 19 5.6511868779977235 1108 20 5.896538289864129 1108 21 5.629830249490837 1108 22 2.567891400743651 1108 23 5.396823532193768 1108 24 6.053844459378396 1108 25 6.406679349302955 1109 1 2.882097769709908 1109 2 -12.910820062743158 1109 3 -5.258933252719121 1109 4 1.5923922863410027 1109 5 0.34290848348724623 1109 6 1.7228995192931187 1109 7 2.8875489062989423 1109 8 2.969576910059075 1109 9 2.8751613686960766 1109 10 2.233580551169858 1109 11 0.20261899035498487 1109 12 0.7583466442791043 1109 13 2.232913124060861 1109 14 2.975023472103043 1109 15 3.130446859688824 1109 16 2.6863435335671753 1109 17 3.189421738498023 1109 18 3.17614194770168 1109 19 2.0275013743122194 1109 20 0.8391263324521709 1109 21 -0.025974418813831335 1109 22 2.0603586407108914 1109 23 2.5794422873125242 1109 24 3.5540614945954303 1109 25 4.0024225925462 1110 1 3.2040366306814327 1110 2 8.524690825029039 1110 3 5.953152174416555 1110 4 7.200746557697787 1110 5 8.028205803420246 1110 6 3.816567861001662 1110 7 6.0937716868684575 1110 8 7.173081935184701 1110 9 5.045446125815005 1110 10 4.706599814151431 1110 11 2.949030213135554 1110 12 5.255528386322656 1110 13 9.506229706975432 1110 14 7.010572048317449 1110 15 6.382239154496194 1110 16 6.188181892026137 1110 17 6.479530616219964 1110 18 6.5488220147033545 1110 19 5.639963685870008 1110 20 5.769947102971432 1110 21 4.135729936295046 1110 22 5.758444905367508 1110 23 6.191305771399627 1110 24 6.836130171312348 1110 25 7.283088421691929 1111 1 4.984573139168035 1111 2 9.119557605565545 1111 3 7.508973151221763 1111 4 8.070122171398474 1111 5 8.502403557378873 1111 6 7.677316596468817 1111 7 7.556112124689747 1111 8 7.8177255143283695 1111 9 6.307870728301989 1111 10 5.669249680890712 1111 11 7.4106280215536735 1111 12 6.870904115543471 1111 13 8.496443501729168 1111 14 6.322860089508627 1111 15 5.926900061659268 1111 16 6.024943697770787 1111 17 7.077623708941374 1111 18 6.983251964122044 1111 19 5.7821483365453865 1111 20 5.661622227361857 1111 21 8.400770244568761 1111 22 7.605888197361136 1111 23 7.5443889211384665 1111 24 7.486434236795759 1111 25 7.960267420132407 1112 1 -1.016789254898594 1112 2 7.75277054128369 1112 3 6.187632742806606 1112 4 0.43145828186673185 1112 5 4.425676939863084 1112 6 3.2143312866309266 1112 7 5.793846445448828 1112 8 5.950824113638493 1112 9 4.721716178539477 1112 10 3.47495079725487 1112 11 -0.4330465577434488 1112 12 4.131819798641519 1112 13 8.076134600570928 1112 14 5.732791928478882 1112 15 3.6521358977201466 1112 16 4.051494540278586 1112 17 5.099813237379454 1112 18 5.0955826635853505 1112 19 5.080107638294322 1112 20 4.968209106501387 1112 21 -0.9447943293283743 1112 22 2.633361996415587 1112 23 4.274145866730869 1112 24 5.76495634918156 1112 25 5.494161966147988 1113 1 -17.183735563623426 1113 2 -12.910820062743158 1113 3 -6.869743650272639 1113 4 -5.60435847818499 1113 5 -0.8682612412833954 1113 6 -0.09814023605856192 1113 7 -19.15078442703439 1113 8 -8.603458258136644 1113 9 -4.4894869493467375 1113 10 -3.799393454946351 1113 11 -4.803856544813211 1113 12 -2.734336841959429 1113 13 -1.336903389700609 1113 14 -1.6508251517501655 1113 15 -2.061540907895271 1113 16 -2.254413347166768 1113 17 -2.0905629709820985 1113 18 -2.883827471258563 1113 19 -2.155556729663317 1113 20 -3.2492528724407954 1113 21 -0.7066701374682687 1113 22 -1.5820496161698436 1113 23 -2.102491963452003 1113 24 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0.9645542121304622 2204 6 3.573686788974861 2204 7 4.820739861072813 2204 8 4.691507395821219 2204 9 3.064709107389543 2204 10 2.9218426952234573 2204 11 4.596433898227684 2204 12 4.84179304293907 2204 13 2.5280052513640427 2204 14 3.949433019841738 2204 15 4.11925757995683 2204 16 4.0726040025789025 2204 17 -1.1008713938302854 2204 18 2.7263764535643276 2204 19 3.4682718935751993 2204 20 3.8788248249144814 2204 21 5.3358974404826505 2204 22 4.788595659136352 2204 23 3.6728803107631087 2204 24 5.152713085885214 2204 25 5.288113547320069 2205 1 5.410526731458543 2205 2 9.56622911758471 2205 3 6.763389151652461 2205 4 7.315411412024066 2205 5 7.40426094250364 2205 6 6.849566185959787 2205 7 6.8246254090311815 2205 8 6.402678002955248 2205 9 5.1163635544884265 2205 10 4.635807327126141 2205 11 6.71104795210362 2205 12 6.035026972147957 2205 13 8.96996230438873 2205 14 6.67572019341452 2205 15 5.713944127448389 2205 16 5.1899091073376695 2205 17 5.86485889696797 2205 18 5.839311346608785 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2207 7 3.4534528611012014 2207 8 3.3446757801155726 2207 9 1.145274363046358 2207 10 0.7329732065370891 2207 11 -1.6510109531478392 2207 12 -2.0463567455514067 2207 13 0.4708792257557768 2207 14 -5.12908669738848 2207 15 -1.276219806977842 2207 16 -0.14615646643282432 2207 17 0.9936307780460467 2207 18 1.061678670865522 2207 19 0.5870493969110875 2207 20 0.43206418555951487 2207 21 -3.054858599604792 2207 22 -3.7463786474247023 2207 23 -1.0390181646648777 2207 24 0.5633552799061654 2207 25 1.1815610106252947 2208 1 5.473292548826375 2208 2 6.848216081835156 2208 3 6.565384016357182 2208 4 8.60659911365024 2208 5 4.009705672242266 2208 6 6.748762973108381 2208 7 7.387368183407375 2208 8 7.176092974316102 2208 9 5.590101127732222 2208 10 5.107164888519254 2208 11 6.778833849792736 2208 12 6.606730178415649 2208 13 9.04974043329781 2208 14 4.109290540376136 2208 15 5.253499068524167 2208 16 5.607257188185649 2208 17 6.47261450958236 2208 18 1.8517644376615188 2208 19 4.0125616152760735 2208 20 5.21623476618711 2208 21 7.469959316125927 2208 22 6.673722094526152 2208 23 2.587283652774371 2208 24 3.76857956688459 2208 25 5.02421978130122 2209 1 2.8675464876586254 2209 2 4.133844949663791 2209 3 4.581972454476908 2209 4 0.4153348008157778 2209 5 4.750379533942169 2209 6 4.981162546588404 2209 7 5.777255275198858 2209 8 4.345939606502416 2209 9 4.066244908811875 2209 10 3.1991078464552167 2209 11 -0.7753152528211942 2209 12 2.86343142508969 2209 13 1.8408452994123898 2209 14 3.203999898902051 2209 15 3.6912739895482147 2209 16 3.0839510604488964 2209 17 1.0859602000364852 2209 18 3.1546613852533767 2209 19 3.6801730037580427 2209 20 3.752194984230499 2209 21 5.853171569606349 2209 22 4.524980228800221 2209 23 0.22390258507927285 2209 24 -3.083808646016646 2209 25 -0.21277385079540423 2210 1 -2.3045866274532116 2210 2 -12.910820062743158 2210 3 -7.224075096690752 2210 4 -2.4302495576306278 2210 5 -1.0599106654489248 2210 6 -1.4819231757423417 2210 7 -1.2920610227790732 2210 8 -19.36125642327426 2210 9 -12.37150529797059 2210 10 -7.092288243156382 2210 11 -9.28172498127622 2210 12 -4.566493781252813 2210 13 -5.2060230461519055 2210 14 -4.185242485343764 2210 15 -5.694517679318267 2210 16 -5.2789224238796315 2210 17 -4.79572010547361 2210 18 -4.87754599556073 2210 19 -9.428385150510477 2210 20 -7.140589979604567 2210 21 -5.460318390445039 2210 22 -9.432992423118897 2210 23 -7.690327216233577 2210 24 -9.845973966397477 2210 25 -7.722275989014085 2211 1 0.07772453824414782 2211 2 4.618891312469065 2211 3 0.5244651626742005 2211 4 1.4673569156562287 2211 5 1.1812488909575336 2211 6 0.10185820633330067 2211 7 -19.15078442703439 2211 8 -12.619320939403291 2211 9 -8.784849949923052 2211 10 -7.238226183408525 2211 11 -9.453757636696514 2211 12 -4.858805308182696 2211 13 -6.264896723137781 2211 14 -4.612442572039569 2211 15 -5.048376003921813 2211 16 -11.386903495295302 2211 17 -7.518758793477076 2211 18 -5.7729865186254266 2211 19 -8.37089138177154 2211 20 -6.416278308181674 2211 21 -4.272486869294873 2211 22 -5.995197386453796 2211 23 -7.5676389237797235 2211 24 -20.2442718387379 2211 25 -20.016744074120467 2212 1 -0.44433069437184614 2212 2 -12.910820062743158 2212 3 -7.164714737540281 2212 4 -18.30344104699233 2212 5 -18.364591516512753 2212 6 -19.250433814040214 2212 7 -9.078647366619604 2212 8 -8.531680228504197 2212 9 -4.379404306086009 2212 10 -4.8604328242434285 2212 11 -4.505892431346247 2212 12 -2.417219180788525 2212 13 -4.576834396227687 2212 14 -3.1515928489068763 2212 15 -3.158365884525182 2212 16 -3.26770741323174 2212 17 -3.521355237756861 2212 18 -2.4923010940776997 2212 19 -2.998275601942666 2212 20 -3.17959924614717 2212 21 -1.04358412725988 2212 22 -2.2769024052764877 2212 23 -2.394195374244436 2212 24 -1.4128922174574683 2212 25 -1.8616493942286745 2213 1 -17.183735563623426 2213 2 -12.910820062743158 2213 3 -7.788743858779728 2213 4 -4.918024380325663 2213 5 -18.364591516512753 2213 6 -9.63774442010082 2213 7 -19.15078442703439 2213 8 -9.539286726304562 2213 9 -7.473626510091801 2213 10 -6.483427024587719 2213 11 -5.765032524796531 2213 12 -4.329494264811807 2213 13 -17.65708687593914 2213 14 -11.081150770321198 2213 15 -5.9033031403111735 2213 16 -5.352785254311613 2213 17 -3.804555534229248 2213 18 -3.7665474462377126 2213 19 -4.668786504475662 2213 20 -4.584547909972073 2213 21 -3.060254721844135 2213 22 -3.7906262077739576 2213 23 -3.4806334702632356 2213 24 -2.8105597175257806 2213 25 -3.6616304377568305 2214 1 -17.183735563623426 2214 2 -12.910820062743158 2214 3 -11.35602726981314 2214 4 -18.30344104699233 2214 5 -18.364591516512753 2214 6 -19.250433814040214 2214 7 -15.079203230453196 2214 8 -13.714888901906738 2214 9 -12.724112135577428 2214 10 -12.553235688146382 2214 11 -13.17188955665356 2214 12 -11.18188839845594 2214 13 -17.65708687593914 2214 14 -15.357690203110632 2214 15 -21.97621980697784 2214 16 -16.691241936518296 2214 17 -21.70891159483531 2214 18 -16.287405060845327 2214 19 -13.606772129961286 2214 20 -12.329036060710223 2214 21 -10.047021427360841 2214 22 -10.995560162707914 2214 23 -10.898271387901152 2214 24 -9.803032522498585 2214 25 -9.555205612582006 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 152519.0 3 5.0 4 791.0 5 2789.0 6 3406.0 7 3377.0 8 2882.0 9 2870.0 10 2598.0 11 2432.0 12 2360.0 13 2434.0 14 3632.0 15 7101.0 16 10044.0 17 10620.0 18 11302.0 19 13452.0 20 15920.0 21 18383.0 22 19049.0 23 20585.0 24 21460.0 25 21365.0 26 17910.0 27 15083.0 28 16061.0 29 18044.0 30 17743.0 31 20581.0 32 25486.0 33 31224.0 34 36664.0 35 39920.0 36 37802.0 37 36893.0 38 21834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.15513416216668 18.658964376909875 12.986227971032694 26.199673489890746 2 13.086636828644501 20.36490683229814 42.27187614176105 24.276580197296312 3 19.06622994845924 25.19429802475866 29.000491230987897 26.7389807957942 4 11.202678688845204 17.275418713730303 38.608154085303966 32.91374851212053 5 11.863136149477164 37.677563801945446 36.35353581195676 14.105764236620628 6 30.209614600517597 37.834693289159645 19.48816983439986 12.467522275922896 7 26.592628380558285 30.771045776077788 24.312411819373974 18.323914023989946 8 24.6918121241876 38.29028148536374 20.715245295290476 16.302661095158186 9 26.534507091536813 15.194171643183726 20.2452878604087 38.026033404870766 10 14.231649071370306 29.43463534585693 35.477821651081136 20.85589393169163 11 33.18177127375281 21.652734971250958 25.733666646643222 19.431827108353012 12 24.35463583629244 24.301016450489374 32.48191311496182 18.862434598256367 13 28.816405904711395 21.455448616289086 24.97456509342887 24.753580385570654 14 19.853869411881362 23.04657265016716 28.091585429563843 29.007972508387635 15 21.868048628662713 32.879245820739925 23.41240615883063 21.840299391766727 16 20.765276573270235 28.09845186187691 29.758371619168223 21.37789994568463 17 20.574995651469806 28.64976637896391 30.02930009656736 20.745937872998926 18 20.43330193464242 27.107914392479692 32.52618488160426 19.93259879127363 19 22.728072107007794 26.57874474810873 27.333775701569913 23.359407443313565 20 23.175323195915436 28.065030581665024 28.53806789304888 20.221578329370665 21 21.463249235578626 25.977554672663437 26.803631514687076 25.755564577070857 22 22.40256135847917 29.156323686793108 28.004718915182387 20.436396039545336 23 21.146931979553337 28.64151559597715 28.910803899212983 21.30074852525653 24 22.567930735154214 27.228669444862636 29.242327662695832 20.96107215728732 25 21.901731988644897 27.794634174079512 29.0870280917798 21.216605745495794 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 613.0 1 613.0 2 1399.0 3 2185.0 4 2185.0 5 2185.0 6 3133.0 7 4081.0 8 4081.0 9 4081.0 10 4687.5 11 5294.0 12 5294.0 13 5294.0 14 6584.0 15 7874.0 16 7874.0 17 7874.0 18 11421.0 19 14968.0 20 14968.0 21 14968.0 22 21420.0 23 27872.0 24 27872.0 25 27872.0 26 37230.5 27 46589.0 28 46589.0 29 46589.0 30 56794.0 31 66999.0 32 66999.0 33 66999.0 34 73336.0 35 79673.0 36 79673.0 37 79673.0 38 82625.0 39 85577.0 40 85577.0 41 85577.0 42 85456.5 43 85336.0 44 85336.0 45 85336.0 46 83249.0 47 81162.0 48 81162.0 49 81162.0 50 73874.5 51 66587.0 52 66587.0 53 66587.0 54 56839.5 55 47092.0 56 47092.0 57 47092.0 58 38837.0 59 30582.0 60 30582.0 61 30582.0 62 24279.0 63 17976.0 64 17976.0 65 17976.0 66 13610.0 67 9244.0 68 9244.0 69 9244.0 70 6760.0 71 4276.0 72 4276.0 73 4276.0 74 2979.5 75 1683.0 76 1683.0 77 1683.0 78 1167.0 79 651.0 80 651.0 81 651.0 82 426.0 83 201.0 84 201.0 85 201.0 86 141.5 87 82.0 88 82.0 89 82.0 90 49.5 91 17.0 92 17.0 93 17.0 94 10.0 95 3.0 96 3.0 97 3.0 98 3.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.01970082476359 2 36.22100110541332 3 5.422496544673116 4 13.61668227450078 5 13.354383276946088 6 14.629613717028752 7 9.264208347836725 8 7.742262470853644 9 0.1067546725194831 10 1.4454844812494811 11 5.44434265773986 12 5.748003629367584 13 15.970673777819206 14 1.590979594273988 15 2.903494067323895 16 2.933641703356 17 2.8733464312917896 18 1.5105858981883746 19 1.450581907631721 20 0.27628050991740716 21 4.213969569820906 22 1.6081652032198257 23 0.1076285170421528 24 9.063952311391583 25 0.11097825437905336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 686621.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.61021612993741 #Duplication Level Percentage of deduplicated Percentage of total 1 97.31373728107044 90.12246242011884 2 1.9093543942764597 3.5365144624517737 3 0.34321180419355374 0.9535475809413231 4 0.13614302030114936 0.504329381386876 5 0.07235476791906051 0.33503953475078274 6 0.04049033727161362 0.22498913315389188 7 0.03232181915917157 0.20953314606305426 8 0.020674623284245977 0.15317450646072453 9 0.014245702438940388 0.11873678236137414 >10 0.10292242463784418 1.7680569004323028 >50 0.007433626611229444 0.5044952726088344 >100 0.0063022216979883175 1.0351558539343089 >500 8.079771383101803E-4 0.5339650253360249 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 946 0.13777615307425786 No Hit GTCCTAAAGTGTGTATTTCTCATTT 780 0.11359978794706249 No Hit CTGTAGGACGTGGAATATGGCAAGA 689 0.10034647935323854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.4564075377828525E-4 3 0.0 0.0 0.0 0.0 1.4564075377828525E-4 4 0.0 1.4564075377828525E-4 0.0 0.0 1.4564075377828525E-4 5 0.0 1.4564075377828525E-4 0.0 0.0 1.4564075377828525E-4 6 0.0 1.4564075377828525E-4 0.0 0.0 1.4564075377828525E-4 7 1.4564075377828525E-4 1.4564075377828525E-4 0.0 0.0 1.4564075377828525E-4 8 1.4564075377828525E-4 1.4564075377828525E-4 0.0 0.0 1.4564075377828525E-4 9 1.4564075377828525E-4 1.4564075377828525E-4 0.0 0.0 1.4564075377828525E-4 10 1.4564075377828525E-4 1.4564075377828525E-4 0.0 0.0 1.4564075377828525E-4 11 1.4564075377828525E-4 1.4564075377828525E-4 0.0 0.0 1.4564075377828525E-4 12 1.4564075377828525E-4 1.4564075377828525E-4 0.0 0.0 1.4564075377828525E-4 13 1.4564075377828525E-4 1.4564075377828525E-4 0.0 1.4564075377828525E-4 1.4564075377828525E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCCCG 25 0.0035196678 20.85078 5 CCATTGG 45 0.0012060227 17.775019 1 GATTAAA 55 2.3579694E-4 16.967064 1 CCCCTAC 50 0.002440562 15.997517 1 TTAGACT 55 6.874881E-5 15.623916 4 GATATAC 145 6.730261E-11 14.710361 1 GTCCTAA 420 0.0 14.600908 1 TGTAGGA 730 0.0 14.249308 2 ATTTAGA 190 0.0 14.03291 1 TCCTGCG 60 0.00688348 13.686834 2 TCCTAAA 475 0.0 13.542763 2 ACTGTTC 145 0.0 13.464854 8 TTTAGAA 245 0.0 13.407512 2 GTATAAG 60 0.008193058 13.3312645 1 GTCTTAG 60 0.008193058 13.3312645 1 CTGTAGG 720 0.0 13.331264 1 TAGGACA 245 0.0 13.152785 4 CTAGGAC 75 5.5002616E-5 13.137634 3 GTCCTAC 630 0.0 12.90805 1 TAGGACC 300 0.0 12.8897295 4 >>END_MODULE