FastQCFastQC Report
Thu 26 May 2016
SRR1926679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686621
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT20370.29667021544636707No Hit
CTGTAGGACGTGGAATATGGCAAGA20020.2915727890641271No Hit
CTTTAGGACGTGAAATATGGCGAGG19270.28064973253075565No Hit
GTCCTAAAGTGTGTATTTCTCATTT18520.26972667599738426No Hit
GTCCTACAGTGTGCATTTCTCATTT12670.18452683503708742No Hit
CTGTAGGACCTGGAATATGGCGAGA12070.1757883898103903No Hit
CTGAAGGACCTGGAATATGGCGAGA10770.15685509181921323No Hit
CCCATGTACTCTGCGTTGATACCAC10130.14753408357740294No Hit
GTACATGGGAAGCAGTGGTATCAAC9270.13500897875247045No Hit
GTATCAACGCAGAGTACTTTTTTTT7480.10893928382615736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA400.005159974614.2974691
GTCCTAG852.5847294E-713.4564421
TAGGACC4550.012.9910334
AGGACCT7850.012.2663425
CTATCCT701.0537121E-412.2576674
GGACCTG7650.012.2131586
TCTAAAC550.002981806212.1356323
AGGACTG1501.2732926E-1112.0760735
GTGTACT951.0586664E-611.979423511
TAGGACT1603.6379788E-1211.9171774
TAGGACA3200.011.9171774
GTATAGG1052.5965528E-711.8010861
TGTAGGA12400.011.7677172
GGACTTG1156.708433E-811.6062936
CTATAGT752.0034902E-411.440494
TTGTCCC752.0034902E-411.440494
TAGGCAG752.0034902E-411.440495
GTAGGAC12050.011.3155583
CTGTAGG12450.011.2542321
GTCCTAA5200.011.1813541
Produced by FastQC (version 0.11.5)