FastQCFastQC Report
Thu 26 May 2016
SRR1926678_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926678_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732670
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10200.1392168370480571No Hit
GTCCTAAAGTGTGTATTTCTCATTT8740.1192897211568646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCCC250.00731623818.3729639
GTTCTAA654.4443048E-516.4404161
TGCAATG455.3377706E-516.09466212
GTAAGGT550.00105211913.936473
TGTAGGA6550.013.8033132
CTGTAGG6650.013.4582741
GTCCTAC6800.013.3578391
GTGTAAG700.001448186813.3578391
CCAACGT450.001554961413.23419419
TTTAAGC500.00901817613.14010053
TCCAACG1300.013.09678418
TCCTAGG1051.0862774E-513.0464212
GACGTGG3050.013.03566557
TATACAG1202.5120244E-913.0264615
GTCCTAA3700.012.9968151
GGACGTG6900.012.8340616
GTGCAGG1152.5489826E-612.7770621
ATATAAC952.7821752E-612.6790453
AGGACGT7250.012.6491435
CCAACGA952.2904715E-812.53765619
Produced by FastQC (version 0.11.5)