##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926678_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732670 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.66268169844541 26.0 2.0 30.0 2.0 31.0 2 15.413035882457313 12.0 2.0 30.0 2.0 31.0 3 18.737468437359247 16.0 7.0 30.0 2.0 31.0 4 20.782514638240954 27.0 2.0 35.0 2.0 35.0 5 20.95593923594524 27.0 2.0 35.0 2.0 35.0 6 21.90589487763932 29.0 2.0 35.0 2.0 36.0 7 21.796657431039897 29.0 2.0 35.0 2.0 37.0 8 21.894099662876876 29.0 2.0 35.0 2.0 37.0 9 24.51172287660202 31.0 10.0 37.0 2.0 39.0 10 25.16313074098844 31.0 8.0 37.0 2.0 39.0 11 22.40787940000273 28.0 2.0 35.0 2.0 39.0 12 23.480992807130086 30.0 2.0 37.0 2.0 39.0 13 20.27610793399484 25.0 2.0 35.0 2.0 39.0 14 23.419583168411428 29.0 2.0 37.0 2.0 40.0 15 24.45774496021401 30.0 2.0 37.0 2.0 40.0 16 24.798314384374958 31.0 2.0 38.0 2.0 40.0 17 24.13447936997557 30.0 2.0 37.0 2.0 40.0 18 24.20138261427382 30.0 2.0 37.0 2.0 40.0 19 24.554043430193676 30.0 2.0 38.0 2.0 40.0 20 24.7916333410676 31.0 2.0 38.0 2.0 40.0 21 22.735595834413857 30.0 2.0 36.0 2.0 40.0 22 23.610674655711303 30.0 2.0 37.0 2.0 40.0 23 23.575998744318724 30.0 2.0 37.0 2.0 40.0 24 22.700297541867418 30.0 2.0 38.0 2.0 40.0 25 22.495283005991784 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.649792324373973 1101 2 9.09959868462456 1101 3 6.04752382539257 1101 4 7.34256553733848 1101 5 6.761325025785624 1101 6 6.6983716223754435 1101 7 6.30105322576777 1101 8 4.972964350652635 1101 9 4.9459206820313355 1101 10 4.697355481863909 1101 11 7.166934825495371 1101 12 5.014223356793941 1101 13 9.02952161281377 1101 14 6.415094130926565 1101 15 5.597526212086443 1101 16 5.22681659603743 1101 17 1.4086412318834185 1101 18 3.8221929690741767 1101 19 4.374190126364354 1101 20 2.158843401490561 1101 21 -0.44745735010241106 1101 22 3.326613936793123 1101 23 4.241463951105128 1101 24 5.699101821603932 1101 25 6.2220070148162065 1102 1 5.073431852178103 1102 2 9.552259388129986 1102 3 6.075856993535439 1102 4 7.530060790794284 1102 5 7.276696105783717 1102 6 6.199192211371699 1102 7 6.18833712124211 1102 8 6.2043903991722935 1102 9 5.487662596172626 1102 10 4.9962455306616 1102 11 7.323489046798247 1102 12 4.85052389703112 1102 13 8.93542418991374 1102 14 6.697885189016542 1102 15 4.541039439981063 1102 16 4.97127424141684 1102 17 5.755444024074244 1102 18 5.996045850017335 1102 19 4.382648950817519 1102 20 5.0324797756585475 1102 21 4.367855419807011 1102 22 5.3037378463751566 1102 23 5.745788294215593 1102 24 7.1316281673377055 1102 25 6.633115022348889 1103 1 3.5269980966964454 1103 2 8.126626869652998 1103 3 5.1494999876397 1103 4 6.775313862185737 1103 5 6.00768343109393 1103 6 5.588995882961296 1103 7 5.7532939463999995 1103 8 5.437479506004745 1103 9 4.665160733753556 1103 10 3.9988554773874547 1103 11 4.573261844471695 1103 12 3.482952773102813 1103 13 7.572358712123247 1103 14 5.736861588134872 1103 15 4.844478067192604 1103 16 4.611856800982249 1103 17 2.582633294492375 1103 18 1.2397808258634875 1103 19 3.3161535499046124 1103 20 3.5489100239042877 1103 21 5.825143942441482 1103 22 1.6324554524434447 1103 23 3.9705573158503746 1103 24 5.115772644977156 1103 25 1.4064695014987585 1104 1 4.688880074367898 1104 2 9.23667471654295 1104 3 6.041047037081487 1104 4 6.664468567129912 1104 5 6.185514372082764 1104 6 6.189474351860817 1104 7 6.283596976703031 1104 8 6.222168501220057 1104 9 5.168350526417033 1104 10 4.134421665585222 1104 11 7.133070456911888 1104 12 4.252490253166609 1104 13 8.392932874802671 1104 14 6.14435760089404 1104 15 5.365211719505204 1104 16 5.120628730806811 1104 17 5.467800758607208 1104 18 3.094645259675289 1104 19 3.926201396794564 1104 20 3.2028175199170477 1104 21 2.2438042295228193 1104 22 4.378069487531164 1104 23 4.744082036743517 1104 24 5.5400150692195815 1104 25 4.44315211712874 1105 1 2.6403739356978235 1105 2 8.385689205074264 1105 3 5.485223797910532 1105 4 5.152831800100568 1105 5 1.8337837041312177 1105 6 4.369906804079292 1105 7 5.381541166536778 1105 8 2.319464647696531 1105 9 3.2032552248247512 1105 10 2.9282859836082977 1105 11 5.817002716771672 1105 12 3.4872631018307416 1105 13 4.612927720336774 1105 14 3.013719903819762 1105 15 3.5506921866940395 1105 16 3.641958134793679 1105 17 4.120621334637445 1105 18 1.5520107299829498 1105 19 1.6205994634963403 1105 20 0.9560866838850899 1105 21 2.09515765033267 1105 22 3.3290516373916574 1105 23 0.8822161200889909 1105 24 3.7486364736994844 1105 25 4.285098637677503 1106 1 6.5963425495873835 1106 2 11.05554591748498 1106 3 7.865891646480939 1106 4 9.206345775017944 1106 5 9.023289566227856 1106 6 8.04351897914696 1106 7 7.752328810535701 1106 8 7.588070511981883 1106 9 6.617738652637428 1106 10 5.985962156574804 1106 11 8.435266047843335 1106 12 5.735307853667436 1106 13 4.7422002658801965 1106 14 6.2498033520604 1106 15 6.242505314580402 1106 16 6.252528147998898 1106 17 6.853888641866757 1106 18 6.953839762499896 1106 19 6.8119091879376725 1106 20 6.128170409362411 1106 21 6.268625735283823 1106 22 6.836453127174025 1106 23 6.978721843567289 1106 24 8.006477529533491 1106 25 6.736881342486104 1107 1 3.5921862810405933 1107 2 -12.915637883138071 1107 3 -5.169146144279093 1107 4 1.7035868469359876 1107 5 4.138416119391149 1107 6 4.268113601441957 1107 7 4.248762463976195 1107 8 4.824983182076053 1107 9 3.0716854094966095 1107 10 2.5381970670854272 1107 11 5.197544171629229 1107 12 2.573650756846341 1107 13 3.9367315102938143 1107 14 3.5853623001639505 1107 15 3.252423750417151 1107 16 2.9355743859650865 1107 17 3.7238847328887417 1107 18 -1.5437147427164462 1107 19 1.1803250030906831 1107 20 1.6783113947585662 1107 21 -1.352417318295391 1107 22 1.633589085719791 1107 23 -1.588136577539288 1107 24 1.7070057182703913 1107 25 2.44432174844777 1108 1 5.85918860181928 1108 2 10.791089252160493 1108 3 1.0875558053947643 1108 4 6.696268847050494 1108 5 7.998982023896282 1108 6 7.939712139692588 1108 7 7.99928771870956 1108 8 7.707566700903971 1108 9 6.604429902346034 1108 10 5.558510386666423 1108 11 7.949959360266956 1108 12 3.622450145682894 1108 13 4.329332314961508 1108 14 5.675727511963199 1108 15 5.7525310278771435 1108 16 5.693059085192054 1108 17 1.0701399398937568 1108 18 4.342092883062762 1108 19 5.321267344492092 1108 20 5.70391514131542 1108 21 5.714403527189788 1108 22 2.7319610707460527 1108 23 5.65680938180169 1108 24 6.378984787888548 1108 25 6.910378986611249 1109 1 3.329049207560285 1109 2 -12.915637883138071 1109 3 -5.077309106978452 1109 4 2.0188285686135394 1109 5 0.7601221388569961 1109 6 2.1619642446689475 1109 7 3.4096605500126245 1109 8 3.691301693609063 1109 9 3.148673956556866 1109 10 2.4834554551330896 1109 11 0.42518128366163666 1109 12 0.885300608865581 1109 13 2.1532051198974393 1109 14 3.2349551306592588 1109 15 3.2048504569407577 1109 16 3.044218360271593 1109 17 3.5148427822710175 1109 18 3.6720459495606796 1109 19 2.3918591988048767 1109 20 1.2497816532948889 1109 21 0.3906608001248024 1109 22 2.8635559309123444 1109 23 3.1680611176381426 1109 24 4.128639378406927 1109 25 4.403135055447052 1110 1 3.603451318726375 1110 2 9.085150138926547 1110 3 6.397666610946299 1110 4 7.670682253074844 1110 5 8.442686692524962 1110 6 3.9997948015989593 1110 7 6.5837749414946565 1110 8 7.5396697548915235 1110 9 5.725881792794883 1110 10 5.338668312721008 1110 11 3.185481465089971 1110 12 5.871063066028334 1110 13 10.276486590383264 1110 14 7.9282001322169755 1110 15 7.270036375169724 1110 16 6.678295733042681 1110 17 7.1765229186171595 1110 18 7.359107726825325 1110 19 6.069325432975713 1110 20 6.413279738218012 1110 21 4.12988414023879 1110 22 6.474824646436378 1110 23 6.9344987081232325 1110 24 7.581361719748763 1110 25 8.085448541620014 1111 1 4.924123559805935 1111 2 8.702260949546755 1111 3 6.910155074241949 1111 4 7.439433038797233 1111 5 7.736585807303289 1111 6 6.971637577399015 1111 7 6.968665768243252 1111 8 7.398209062474628 1111 9 5.8537835997264125 1111 10 5.235996703285593 1111 11 6.687145510126815 1111 12 6.136279404148059 1111 13 7.973743231078931 1111 14 6.1142450893356965 1111 15 5.761367212588816 1111 16 5.538427029170336 1111 17 6.842556714645163 1111 18 6.624849617403708 1111 19 5.461732211805209 1111 20 4.863174106466548 1111 21 7.6985122766126 1111 22 7.008432714310526 1111 23 6.780180684494837 1111 24 6.761158305804145 1111 25 7.209445193292797 1112 1 -1.2616781393482128 1112 2 7.810837395968786 1112 3 5.853646718102219 1112 4 0.5257125862686713 1112 5 4.33304228276857 1112 6 3.2676242721159987 1112 7 5.93431468005231 1112 8 6.077830383197728 1112 9 4.931508421153875 1112 10 3.837770947881875 1112 11 0.18968927668860047 1112 12 4.197466329721632 1112 13 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20 3.845626106211398 2208 21 6.110983354682965 2208 22 5.416660749590932 2208 23 1.1946141046181218 2208 24 2.4292482116882717 2208 25 3.722860329537557 2209 1 3.0512917196894733 2209 2 4.457726243112891 2209 3 4.884504094589836 2209 4 0.7812164519585103 2209 5 4.884555960938382 2209 6 5.323338826189616 2209 7 5.871570427019982 2209 8 4.673074792773001 2209 9 4.266519526494385 2209 10 3.5505625321332843 2209 11 -0.20293515653386152 2209 12 3.4009942551408905 2209 13 1.9977742875935185 2209 14 3.705626666903594 2209 15 4.050098142996223 2209 16 3.663383370923796 2209 17 1.3832012691213684 2209 18 3.566399387804765 2209 19 4.100853595209983 2209 20 4.055958139679767 2209 21 6.02233095784899 2209 22 4.864094806970851 2209 23 0.5638790097555137 2209 24 -3.381919727882348 2209 25 -0.19716929315741893 2210 1 -2.031922673608765 2210 2 -12.915637883138071 2210 3 -7.115075730311304 2210 4 -2.1538494611423467 2210 5 -0.7147984158158351 2210 6 -1.4164735713067849 2210 7 -1.2911712184885076 2210 8 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-6.9314678793455045 2211 21 -4.668004670101276 2211 22 -6.0483947657650425 2211 23 -8.226249558101532 2211 24 -20.228724962678346 2211 25 -20.018570370909536 2212 1 -0.5670695410365738 2212 2 -12.915637883138071 2212 3 -7.005177786243669 2212 4 -18.276520268595764 2212 5 -18.39104065184068 2212 6 -19.32408226695896 2212 7 -9.034067247354093 2212 8 -8.45203520535162 2212 9 -4.489546927454597 2212 10 -4.600394894166332 2212 11 -5.2122313914833 2212 12 -2.204495093893158 2212 13 -5.330711259538136 2212 14 -3.016669870991496 2212 15 -3.098320310197362 2212 16 -3.2004589504530223 2212 17 -3.567092693079932 2212 18 -2.3751576741731952 2212 19 -3.00110991229743 2212 20 -3.0471043654840813 2212 21 -0.9421601393519765 2212 22 -2.33587620991673 2212 23 -2.400500613936149 2212 24 -1.4518936168521108 2212 25 -2.1752995020850037 2213 1 -17.205059319571497 2213 2 -12.915637883138071 2213 3 -8.051018142702992 2213 4 -5.3391304978726595 2213 5 -18.39104065184068 2213 6 -9.97928508882845 2213 7 -19.254680535258693 2213 8 -9.935038489320267 2213 9 -7.832581939427712 2213 10 -6.8968939973093875 2213 11 -5.9657624840263175 2213 12 -4.776390872205745 2213 13 -17.725089453745973 2213 14 -11.428454901743024 2213 15 -6.6760404006942835 2213 16 -5.8277270070226095 2213 17 -4.512752476331993 2213 18 -4.470454358055907 2213 19 -5.285434595543183 2213 20 -5.359450945860189 2213 21 -3.5855460822836314 2213 22 -4.338207737517994 2213 23 -4.136085553950391 2213 24 -3.4412470085337254 2213 25 -4.069716755389255 2214 1 -17.205059319571497 2214 2 -12.915637883138071 2214 3 -11.606878468157275 2214 4 -18.276520268595764 2214 5 -18.39104065184068 2214 6 -19.32408226695896 2214 7 -15.370827844040562 2214 8 -14.09305168935424 2214 9 -13.263748908366164 2214 10 -13.374036254904903 2214 11 -13.840134832902915 2214 12 -12.00491833397211 2214 13 -17.725089453745973 2214 14 -15.680996732660681 2214 15 -22.01995574460963 2214 16 -17.099232020737254 2214 17 -21.707637837884022 2214 18 -16.657797913671416 2214 19 -14.13623224331911 2214 20 -13.061578339677856 2214 21 -10.544354681791003 2214 22 -11.525773625272738 2214 23 -11.410123655183959 2214 24 -10.428913820091 2214 25 -10.131884818501604 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 163493.0 3 4.0 4 810.0 5 2894.0 6 3742.0 7 3648.0 8 3156.0 9 2980.0 10 2834.0 11 2487.0 12 2489.0 13 2616.0 14 3701.0 15 7484.0 16 10921.0 17 11700.0 18 12017.0 19 13945.0 20 16746.0 21 19443.0 22 20277.0 23 21971.0 24 22767.0 25 22765.0 26 19055.0 27 15770.0 28 16782.0 29 19232.0 30 18777.0 31 21864.0 32 26939.0 33 33107.0 34 38775.0 35 42885.0 36 40468.0 37 40093.0 38 24033.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.886930232777736 18.959952031198608 13.09085586854534 26.062261867478316 2 12.84192588109296 20.520167322348783 42.750903038458816 23.887003758099446 3 18.840110672050972 25.613644736937157 29.242272100096077 26.30397249091579 4 11.096423681056484 17.476495719738537 39.00255835354612 32.42452224565886 5 11.575259633024626 38.07851037426941 36.514239162353924 13.831990830352039 6 29.601886952472544 38.39689049690922 19.610255983537172 12.390966567081065 7 26.24717910270249 31.16634223524966 24.390166792374433 18.196311869673416 8 24.4710620719001 38.62650481363064 20.786965078276904 16.115468036192357 9 26.460074933797546 15.286276074474683 20.425881947841486 37.82776704388628 10 14.114480426785114 29.779975271275216 35.56519535831036 20.540348943629308 11 32.90772496528325 21.800675983231915 25.91957851477322 19.372020536711613 12 24.14795626723009 24.667916036554924 32.594950905551975 18.58917679066301 13 28.65988637343227 21.65866162671737 25.18651770190023 24.494934297950135 14 19.672169791698444 23.486132180385653 28.322882665017485 28.51881536289842 15 21.631585758332772 33.34247631265051 23.505474534692684 21.52046339432404 16 20.445230159445096 28.297563282587156 30.105536663363665 21.15166989460409 17 20.290886184874708 28.95391771160536 30.153991951698945 20.601204151820994 18 20.169478374603738 27.294996563878605 32.843916956704874 19.691608104812786 19 22.57209125212033 26.789213140859207 27.675701872814763 22.962993734205696 20 23.044209656071075 28.257825556709875 28.791642884420337 19.90632190279871 21 21.224575854665847 26.2034843384273 26.993864156674135 25.578075650232712 22 22.175770521818734 29.495935861514134 28.12300607540156 20.20528754126557 23 20.8979819386998 28.902042930090005 29.22875324011636 20.97122189109383 24 22.341573040450427 27.6124452232252 29.425910895946256 20.620070840378116 25 21.677168270262218 28.07870531694072 29.37005409999822 20.874072312798837 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 784.0 1 784.0 2 1639.5 3 2495.0 4 2495.0 5 2495.0 6 3495.5 7 4496.0 8 4496.0 9 4496.0 10 5269.0 11 6042.0 12 6042.0 13 6042.0 14 7614.0 15 9186.0 16 9186.0 17 9186.0 18 13347.0 19 17508.0 20 17508.0 21 17508.0 22 24769.0 23 32030.0 24 32030.0 25 32030.0 26 41956.5 27 51883.0 28 51883.0 29 51883.0 30 62751.5 31 73620.0 32 73620.0 33 73620.0 34 80108.0 35 86596.0 36 86596.0 37 86596.0 38 89085.5 39 91575.0 40 91575.0 41 91575.0 42 91055.5 43 90536.0 44 90536.0 45 90536.0 46 87860.0 47 85184.0 48 85184.0 49 85184.0 50 77088.5 51 68993.0 52 68993.0 53 68993.0 54 58213.0 55 47433.0 56 47433.0 57 47433.0 58 38933.0 59 30433.0 60 30433.0 61 30433.0 62 24193.5 63 17954.0 64 17954.0 65 17954.0 66 13504.5 67 9055.0 68 9055.0 69 9055.0 70 6659.5 71 4264.0 72 4264.0 73 4264.0 74 2945.0 75 1626.0 76 1626.0 77 1626.0 78 1126.0 79 626.0 80 626.0 81 626.0 82 433.0 83 240.0 84 240.0 85 240.0 86 160.5 87 81.0 88 81.0 89 81.0 90 50.5 91 20.0 92 20.0 93 20.0 94 13.0 95 6.0 96 6.0 97 6.0 98 5.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.274325412532246 2 36.40697722030382 3 5.5325044017088185 4 13.680374520589078 5 13.549892857630311 6 14.70607504060491 7 9.339948407878035 8 7.621712367095691 9 0.11246536639960693 10 1.423969863649392 11 5.546972033794205 12 5.685369948271391 13 16.08486767576126 14 1.5701475425498517 15 2.9675024226459388 16 3.0308324347932905 17 2.929559010195586 18 1.491258001555953 19 1.4324320635483916 20 0.28430261918735583 21 4.194111946715438 22 1.6009936260526567 23 0.11314780187533267 24 9.021933476189826 25 0.11969918244230009 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 732670.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.78909329425605 #Duplication Level Percentage of deduplicated Percentage of total 1 97.01898578288271 89.05284737339122 2 2.076594664030489 3.8121748270209768 3 0.39958861664721707 1.1003363043826238 4 0.15425519532484638 0.5663577805918406 5 0.09384505226199336 0.43069761286402225 6 0.05158106787195598 0.2840747670669793 7 0.037425424200382806 0.24046720274542321 8 0.02491156499231574 0.1829287970548474 9 0.01953749021021507 0.16139956604769407 >10 0.10685392693105826 1.845709217173059 >50 0.009054930332392413 0.6048950593673424 >100 0.006598963033802683 1.1662084982318548 >500 6.138570244856614E-4 0.40822227585295456 >1k 1.5346425612141536E-4 0.14368071820911446 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1020 0.1392168370480571 No Hit GTCCTAAAGTGTGTATTTCTCATTT 874 0.1192897211568646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.36487095145154E-4 0.0 0.0 0.0 0.0 11 1.36487095145154E-4 0.0 0.0 0.0 1.36487095145154E-4 12 1.36487095145154E-4 0.0 0.0 0.0 1.36487095145154E-4 13 1.36487095145154E-4 0.0 0.0 0.0 1.36487095145154E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGCCC 25 0.007316238 18.372963 9 GTTCTAA 65 4.4443048E-5 16.440416 1 TGCAATG 45 5.3377706E-5 16.094662 12 GTAAGGT 55 0.001052119 13.93647 3 TGTAGGA 655 0.0 13.803313 2 CTGTAGG 665 0.0 13.458274 1 GTCCTAC 680 0.0 13.357839 1 GTGTAAG 70 0.0014481868 13.357839 1 CCAACGT 45 0.0015549614 13.234194 19 TTTAAGC 50 0.009018176 13.1401005 3 TCCAACG 130 0.0 13.096784 18 TCCTAGG 105 1.0862774E-5 13.046421 2 GACGTGG 305 0.0 13.0356655 7 TATACAG 120 2.5120244E-9 13.026461 5 GTCCTAA 370 0.0 12.996815 1 GGACGTG 690 0.0 12.834061 6 GTGCAGG 115 2.5489826E-6 12.777062 1 ATATAAC 95 2.7821752E-6 12.679045 3 AGGACGT 725 0.0 12.649143 5 CCAACGA 95 2.2904715E-8 12.537656 19 >>END_MODULE