FastQCFastQC Report
Thu 26 May 2016
SRR1926678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732670
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA21400.2920823836106296No Hit
GTCCTAAAGTGTGTATTTCTCATTT21350.29139994813490383No Hit
GTCCTACAGTGGACATTTCTAAATT21200.28935264170772657No Hit
CTTTAGGACGTGAAATATGGCGAGG20680.2822553127601785No Hit
GTCCTACAGTGTGCATTTCTCATTT16080.21947124899340764No Hit
CTGTAGGACCTGGAATATGGCGAGA12910.17620483983239385No Hit
CCCATGTACTCTGCGTTGATACCAC12010.16392100126932999No Hit
CTGAAGGACCTGGAATATGGCGAGA11880.16214666903244299No Hit
GTATCAACGCAGAGTACTTTTTTTT11820.16132774646157205No Hit
GTACATGGGAAGCAGTGGTATCAAC10950.14945336918394367No Hit
GGTATCAACGCAGAGTACTTTTTTT7420.10127342459770429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT451.611721E-619.065776
GCGCCAC508.834673E-515.17344713
TAGGACC4450.014.7823424
AAAGGCG602.4807356E-514.3003095
GGACTAT500.00145970113.3460396
CGCCACC500.00151809513.27676614
TGCGCCA655.534855E-513.13086712
AGGACCT8200.012.9051575
TTAGGCA603.967652E-412.7113864
TAAACCC603.967652E-412.7113864
TGGCGAA604.1721232E-412.63590918
GGCGAGG4950.012.63504719
GGACCTG7950.012.5906036
CTTAGGG701.0534324E-412.2582493
AGGCGTG701.08908345E-412.2146387
AATACGG701.1073062E-412.19294814
CCAACGA1103.9357474E-812.06072619
TCCAACG1501.4551915E-1112.00411318
TGTAGGA12650.011.9083212
CTGTAGG12850.011.86403751
Produced by FastQC (version 0.11.5)