##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926678_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732670 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.187677945050297 31.0 28.0 31.0 18.0 34.0 2 28.720029481212553 31.0 28.0 33.0 23.0 34.0 3 29.061273151623514 31.0 28.0 33.0 24.0 34.0 4 32.795452249989765 35.0 33.0 37.0 27.0 37.0 5 32.43579647044372 35.0 33.0 37.0 25.0 37.0 6 32.54340562599806 35.0 33.0 37.0 25.0 37.0 7 32.36226814254712 35.0 33.0 37.0 23.0 37.0 8 32.34007670574747 35.0 33.0 37.0 23.0 37.0 9 33.524574501480885 37.0 33.0 39.0 19.0 39.0 10 33.39731120422564 37.0 33.0 39.0 19.0 39.0 11 33.64784009171933 37.0 33.0 39.0 23.0 39.0 12 33.433538973890016 37.0 33.0 39.0 20.0 39.0 13 33.584318997638775 37.0 33.0 39.0 23.0 39.0 14 34.20756547968389 38.0 33.0 40.0 18.0 41.0 15 34.35979909099595 38.0 33.0 40.0 19.0 41.0 16 34.3446667667572 38.0 33.0 40.0 19.0 41.0 17 34.251215417582266 38.0 33.0 40.0 18.0 41.0 18 34.22409133716407 38.0 33.0 40.0 18.0 41.0 19 34.25033917043144 39.0 34.0 40.0 18.0 41.0 20 34.23541840119017 39.0 34.0 40.0 18.0 41.0 21 34.13889882211637 39.0 34.0 40.0 18.0 41.0 22 34.0718413474006 39.0 34.0 40.0 18.0 41.0 23 33.73110540898358 39.0 33.0 40.0 10.0 41.0 24 33.826290144266856 39.0 34.0 40.0 8.0 41.0 25 33.62370371381386 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5770902530957187 1101 2 0.5742381259600009 1101 3 0.6316472893508269 1101 4 0.4315872273214012 1101 5 0.4150730921939356 1101 6 0.1300520928811011 1101 7 0.33139950568074994 1101 8 0.5188006829847112 1101 9 0.8192559463577425 1101 10 0.748546504061018 1101 11 0.4181215701171226 1101 12 0.3895548909107376 1101 13 0.13486705250326025 1101 14 0.6023486715412005 1101 15 0.5625095353054732 1101 16 0.5586695218942737 1101 17 0.47258663790331923 1101 18 0.5996144469590945 1101 19 0.26926462959707465 1101 20 0.34112142950066726 1101 21 0.5169520455929444 1101 22 0.6717684340988441 1101 23 0.9924412170434991 1101 24 0.6456684386365623 1101 25 0.9266400929484959 1102 1 0.5584001319574767 1102 2 0.5182668768240326 1102 3 0.6225068933313587 1102 4 0.1508022679775607 1102 5 0.49193235377968847 1102 6 0.21001993866443058 1102 7 0.30862252954154457 1102 8 0.6646814549433486 1102 9 0.9205365800712713 1102 10 0.709247048932113 1102 11 0.6042890783970236 1102 12 0.6878901571870344 1102 13 0.3930011811535934 1102 14 0.8565150110449338 1102 15 0.738934882301983 1102 16 0.6147012693562388 1102 17 0.7138372284055947 1102 18 0.6981070088829924 1102 19 0.7671883785367442 1102 20 0.8583608780133787 1102 21 0.6987488716190668 1102 22 0.8055289456829868 1102 23 1.003337351715345 1102 24 0.7438733117123633 1102 25 0.9357978661467072 1103 1 0.06593812914656638 1103 2 0.16608919052452364 1103 3 0.181665259113025 1103 4 -0.1234315801770407 1103 5 0.11653522971313635 1103 6 -0.30334467818924793 1103 7 -0.014166055813568335 1103 8 -0.08755664797482865 1103 9 0.38195954504243446 1103 10 0.4333709439607958 1103 11 0.0762132709007588 1103 12 0.07290401972898763 1103 13 0.018881510168164084 1103 14 0.34729379661969517 1103 15 0.38086529566941607 1103 16 0.4217405734387114 1103 17 0.37674321656291454 1103 18 0.39542614943098897 1103 19 0.5500533550741054 1103 20 0.396653750132387 1103 21 0.3760972476664364 1103 22 0.4208440465096146 1103 23 0.550626155952699 1103 24 0.5048846244682039 1103 25 0.5631041297628485 1104 1 0.2820465820971272 1104 2 0.5584336881321796 1104 3 0.3985711602294515 1104 4 0.1110181805885091 1104 5 0.07267558059032808 1104 6 -0.24911820291013953 1104 7 0.18838577831721182 1104 8 0.21228386239199892 1104 9 0.635548061788846 1104 10 0.3895112948379875 1104 11 0.3026885500076162 1104 12 0.35839046311016887 1104 13 0.32829139852542255 1104 14 0.5514405270822138 1104 15 0.4430662526072169 1104 16 0.4895236675375969 1104 17 0.5753078098643485 1104 18 0.3093017475171109 1104 19 0.2988571828253015 1104 20 0.38947671663956385 1104 21 0.5985852859439547 1104 22 0.39851549786527585 1104 23 0.7252673042780629 1104 24 0.49611269464364227 1104 25 0.6500259798425887 1105 1 0.606589310004825 1105 2 0.4767876953559025 1105 3 0.5748352182881611 1105 4 0.19675039487982104 1105 5 0.46107952637132854 1105 6 -0.07038336282931112 1105 7 0.05421278995958545 1105 8 0.15182720659453253 1105 9 0.6930633104171235 1105 10 0.6610227859353301 1105 11 0.3316263938721207 1105 12 0.3211120958059155 1105 13 0.4529043466931597 1105 14 0.7042200167900958 1105 15 0.5843023532911218 1105 16 0.6143065636751075 1105 17 0.6175202709724132 1105 18 0.3696855684703877 1105 19 0.44643738519411613 1105 20 0.6812213539407139 1105 21 0.5632902674737821 1105 22 0.6861085490476313 1105 23 0.916247735983255 1105 24 0.6578844862687561 1105 25 0.7510273655721917 1106 1 0.8237039327805462 1106 2 0.8042899387842972 1106 3 0.567309701570057 1106 4 0.7366870681855815 1106 5 0.7574674242606818 1106 6 -0.45692425159174377 1106 7 -0.3040645488601186 1106 8 -0.15440441735579924 1106 9 -0.12952892549670025 1106 10 -0.17451932120935254 1106 11 -0.3004878276399907 1106 12 -0.28089093920615227 1106 13 -0.38031955110864857 1106 14 -0.2329913469954974 1106 15 -0.148986841044497 1106 16 -0.047386258083591315 1106 17 -0.12487031173531449 1106 18 -0.013083442305358517 1106 19 -0.10244929066513464 1106 20 -0.08315665097659064 1106 21 -0.09690875927955744 1106 22 -0.1612744826084196 1106 23 0.2507483816528904 1106 24 -0.009659488575664454 1106 25 -0.00419453320724017 1107 1 0.594131918202919 1107 2 0.7826624453211117 1107 3 0.6296090387122604 1107 4 0.6676113746399253 1107 5 0.7035211441061335 1107 6 0.29042229122705976 1107 7 0.6334704591366034 1107 8 0.8140979196886846 1107 9 1.1408013354752526 1107 10 0.6399216115109425 1107 11 0.6569257636858907 1107 12 0.5245629193603492 1107 13 0.4232316600786561 1107 14 1.1016022742197649 1107 15 1.0242088914955971 1107 16 0.688024196525781 1107 17 0.8659346517133528 1107 18 1.076811134464343 1107 19 1.0421954094518995 1107 20 0.8171572590263452 1107 21 1.0141741468072425 1107 22 0.9686805641443854 1107 23 1.0371275000830096 1107 24 1.107186180681893 1107 25 1.0432161263851327 1108 1 1.5830461951268155 1108 2 1.323484844160781 1108 3 1.3267031785870351 1108 4 0.9789889733713295 1108 5 -0.2041745534026589 1108 6 0.5067451206223552 1108 7 1.069032561219636 1108 8 1.344707273010897 1108 9 1.5306158177588571 1108 10 1.615894352048116 1108 11 1.270488641867857 1108 12 1.2458236926486634 1108 13 1.1732121419424075 1108 14 1.5581040111893074 1108 15 1.3790063915548743 1108 16 1.4097788509506728 1108 17 1.393625248482543 1108 18 1.4719865981718883 1108 19 1.6514359602161122 1108 20 1.378531243288073 1108 21 1.6088399992061824 1108 22 1.673593524823005 1108 23 2.012985155905689 1108 24 1.8598720208089858 1108 25 1.7163570311360488 1109 1 0.5083920440607024 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0.5639181889674845 2202 21 0.77605957294994 2202 22 0.5935725750607759 2202 23 0.8890857623774977 2202 24 0.7894832003639181 2202 25 0.7869208297088477 2203 1 -0.2853031303421574 2203 2 -0.0446431273094241 2203 3 -0.23612113879223173 2203 4 -0.4934565984127559 2203 5 -0.05749698175119988 2203 6 -0.1873817222801648 2203 7 0.051073107377625604 2203 8 -0.02037147804905004 2203 9 0.19141737098236433 2203 10 0.28837271246891305 2203 11 0.06022997390929419 2203 12 0.2381429237981152 2203 13 0.07326021966252227 2203 14 0.4114958237316415 2203 15 0.39615349771207775 2203 16 0.29225921921496223 2203 17 0.315455664916648 2203 18 0.405095937222967 2203 19 0.577638194878368 2203 20 0.4497206541548664 2203 21 0.5155722922678265 2203 22 0.481394395870943 2203 23 0.6611864167331518 2203 24 0.6219579184408985 2203 25 0.6858353541016626 2204 1 0.024730604033507575 2204 2 0.29483741546860287 2204 3 0.18346248371863894 2204 4 -0.2812226550022885 2204 5 0.05172211484438094 2204 6 -0.20996206168500464 2204 7 -0.05356261798142725 2204 8 -0.04582207019771545 2204 9 0.38396222027183313 2204 10 0.2661766001601009 2204 11 0.08613922125039863 2204 12 0.08981154446736639 2204 13 0.05808261323193875 2204 14 0.3582313883387229 2204 15 0.4146790962062141 2204 16 0.20983105664109303 2204 17 0.1656586246103373 2204 18 0.18798409087818868 2204 19 0.3590142495149493 2204 20 0.32435511360014146 2204 21 0.2674881796129469 2204 22 0.24814437535550837 2204 23 0.37592006274930867 2204 24 0.4444952549455863 2204 25 0.3931102870368619 2205 1 0.5482633999364808 2205 2 0.5705495187013518 2205 3 0.5857647786871958 2205 4 0.3233889010315565 2205 5 0.18754358579575836 2205 6 0.05606716709602466 2205 7 0.025928953041528757 2205 8 0.175438577120822 2205 9 0.31607100187749637 2205 10 0.3774774106294174 2205 11 0.35908109998266013 2205 12 0.4276167916196769 2205 13 0.20066299958607203 2205 14 0.7186049873125455 2205 15 0.5325394475857408 2205 16 0.5498606505416745 2205 17 0.6036453505929131 2205 18 0.5933730873278762 2205 19 0.7389421624243298 2205 20 0.6498790121161235 2205 21 0.6376773691916711 2205 22 0.5652200800122245 2205 23 0.7172377840189625 2205 24 0.7141450524483588 2205 25 0.6947356695716564 2206 1 0.3781390860843672 2206 2 0.5288108342059203 2206 3 0.5074228348705994 2206 4 0.2989344954398092 2206 5 0.4324888839020886 2206 6 0.28500788771749797 2206 7 0.4793136327125609 2206 8 0.6073959742223778 2206 9 0.9997473300128803 2206 10 0.9285158918921255 2206 11 0.7173381419017844 2206 12 0.6018936059941211 2206 13 0.7009811442801848 2206 14 1.0604704548427932 2206 15 0.8564962178947653 2206 16 0.8483753421787483 2206 17 0.724970067111748 2206 18 0.6959937448902238 2206 19 0.6666449677957473 2206 20 0.7849171894794154 2206 21 0.629685764412848 2206 22 0.5959542819913679 2206 23 1.0683341961065622 2206 24 0.7080931806179365 2206 25 0.533082551753651 2207 1 -0.7394667832354784 2207 2 0.08963297669946968 2207 3 0.4097785056201495 2207 4 0.4087887460736326 2207 5 0.5642393517410724 2207 6 0.5393616797338652 2207 7 0.45972464248246325 2207 8 0.5353143427737592 2207 9 0.7975339167432196 2207 10 0.5306000807785765 2207 11 0.42719497456420186 2207 12 0.490659814371206 2207 13 0.3623321108195512 2207 14 -5.018219325488047 2207 15 -1.746395777078881 2207 16 -0.6270228435158316 2207 17 -0.52120115335169 2207 18 0.07327545896375653 2207 19 0.2883624102595448 2207 20 0.3428972562187056 2207 21 0.46832127296182335 2207 22 0.3564489986690589 2207 23 0.6589074556348606 2207 24 0.27365949325979244 2207 25 0.3699069516779119 2208 1 0.22638208553530248 2208 2 0.40681395325101377 2208 3 0.36260755524082455 2208 4 0.4245198931411238 2208 5 0.5865851682704104 2208 6 0.4223070069102377 2208 7 0.4574134144716808 2208 8 0.6641755140248051 2208 9 0.803914208153266 2208 10 0.8274410671077135 2208 11 0.8237490253390121 2208 12 0.6101618438720635 2208 13 0.7009350045598808 2208 14 0.9349346573407971 2208 15 0.8773617529078948 2208 16 0.8362567446416804 2208 17 0.6046042252694832 2208 18 0.743754122866811 2208 19 0.39874737029098384 2208 20 0.48968065420329054 2208 21 0.7991500361204373 2208 22 0.7356791969789356 2208 23 1.2519229896912947 2208 24 1.0364949468464317 2208 25 0.9452648221047326 2209 1 -0.41138524437225854 2209 2 0.05344457247088741 2209 3 0.27231283122407035 2209 4 0.36739868675629594 2209 5 0.3936217629683867 2209 6 0.4947351844949708 2209 7 0.8061999968364262 2209 8 0.6712972366843317 2209 9 0.943062859701115 2209 10 0.5817184029925855 2209 11 0.6042915497387256 2209 12 0.5968385147951238 2209 13 0.5420830159271048 2209 14 0.790997271136348 2209 15 0.6787904570928163 2209 16 0.7246364337066424 2209 17 0.7796626503563004 2209 18 0.6107537293507619 2209 19 0.7115467379118456 2209 20 0.6105761269330898 2209 21 0.7340575483762208 2209 22 0.9207206538373001 2209 23 0.9578127661902442 2209 24 0.9790863475455822 2209 25 0.8555466048356877 2210 1 -0.2763231564785613 2210 2 -0.44567861832566535 2210 3 0.24961799668818685 2210 4 0.3742738989357619 2210 5 0.5335263240497667 2210 6 0.20906034622950642 2210 7 0.3543689179845657 2210 8 0.4066383256251811 2210 9 0.7998285753256553 2210 10 0.8802638679704273 2210 11 0.624990104288571 2210 12 0.4805832969734851 2210 13 0.34446325108171294 2210 14 0.8179219480258837 2210 15 0.4073346310632431 2210 16 0.4286711904951872 2210 17 0.5948477664752474 2210 18 0.6348698674216138 2210 19 0.5915218979728536 2210 20 0.6262965329788273 2210 21 0.6238832145599602 2210 22 0.9339660313135241 2210 23 1.0035903351754598 2210 24 0.7614338532714413 2210 25 0.8491292221380533 2211 1 -1.4260155932183913 2211 2 -0.553752653196149 2211 3 0.16527526336949805 2211 4 0.2848234186178118 2211 5 0.5431276901099622 2211 6 0.3470294301303696 2211 7 0.3465600066386756 2211 8 0.21598904759997595 2211 9 0.4226217090757274 2211 10 0.4640598776367 2211 11 0.2878113980514314 2211 12 0.4491828855576969 2211 13 0.283504812065118 2211 14 0.39420862095033016 2211 15 0.19231495899757078 2211 16 0.21658485309429665 2211 17 0.2529682354093552 2211 18 0.3224160518397454 2211 19 0.4221898494817111 2211 20 0.5335981646618393 2211 21 0.4563061739576142 2211 22 0.39765009993607947 2211 23 0.7020310493613167 2211 24 0.5267695765443889 2211 25 0.4565691574299393 2212 1 1.0038227586388366 2212 2 0.16449972825183679 2212 3 0.4004785149566743 2212 4 0.43700602459841065 2212 5 0.5181094766204808 2212 6 0.5505932485542004 2212 7 0.7847656081768264 2212 8 0.6959925533543441 2212 9 0.7777601042071112 2212 10 0.6355994515779884 2212 11 0.44999909833133955 2212 12 0.7183275910469362 2212 13 0.5559608121089354 2212 14 0.44856835517032323 2212 15 0.33703825090092465 2212 16 -0.3326970150345616 2212 17 0.3138738703617321 2212 18 0.37532692351270214 2212 19 0.35371808449872333 2212 20 0.14696960481477817 2212 21 0.46659063305806114 2212 22 0.558130114397315 2212 23 0.6748011978628838 2212 24 0.6648497378090923 2212 25 0.3959524733257993 2213 1 0.4231822495485815 2213 2 0.2024216040499205 2213 3 -0.20957800055128928 2213 4 -0.8752564284931204 2213 5 -1.67696366904892 2213 6 -0.49224706884602654 2213 7 -0.17208610847046657 2213 8 -0.1357663979389585 2213 9 -0.8102760945616296 2213 10 -0.6732212942103857 2213 11 -0.49544736216338947 2213 12 -0.6095554960638623 2213 13 -0.7480526602747801 2213 14 -8.011308939176022 2213 15 -3.4945133867268083 2213 16 -1.9597096852045297 2213 17 -1.2808519498041804 2213 18 -4.9739695620699855 2213 19 -2.7433738155460468 2213 20 -5.136585914686226 2213 21 -2.194133041862095 2213 22 -1.704587377167421 2213 23 -5.015359515296126 2213 24 -2.5271272233331175 2213 25 -1.7652504266618863 2214 1 0.5580878957856754 2214 2 0.2492587420107597 2214 3 -1.6253116216673469 2214 4 -1.5753203802095719 2214 5 -0.22125522892121552 2214 6 -0.3248034887489979 2214 7 -0.36875262525029484 2214 8 -0.21181398201298407 2214 9 -4.502948127177003 2214 10 -1.327769610231794 2214 11 -0.8468770327620874 2214 12 -1.045346473875611 2214 13 -1.1707010607324335 2214 14 -2.3429235701296527 2214 15 -1.840432218009191 2214 16 -1.173790554148475 2214 17 -1.4268767011169388 2214 18 -0.8992659632870641 2214 19 -4.204160921281215 2214 20 -2.0948499253581687 2214 21 -3.830032937440933 2214 22 -5.797417290582793 2214 23 -5.8523201100470885 2214 24 -5.5655731966232835 2214 25 -3.2828121675475153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26673.0 3 10.0 4 455.0 5 3164.0 6 3944.0 7 3137.0 8 2372.0 9 1932.0 10 1733.0 11 1608.0 12 1475.0 13 1479.0 14 1418.0 15 1465.0 16 1482.0 17 1613.0 18 1776.0 19 2129.0 20 2773.0 21 3597.0 22 4534.0 23 5635.0 24 6713.0 25 7876.0 26 8988.0 27 10078.0 28 11739.0 29 13927.0 30 17130.0 31 21437.0 32 27358.0 33 35258.0 34 45771.0 35 59575.0 36 80996.0 37 119151.0 38 192269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.27253353733505 20.085243572579984 9.29919947542417 30.343023414660802 2 12.582877303360968 21.790586667540495 41.206404080785575 24.420131948312964 3 17.661105998585484 26.40202292171282 29.30246338779311 26.634407691908585 4 11.648721050576325 18.596992345691824 38.387051848858135 31.367234754873717 5 12.332230685111792 39.7255074889519 35.704231793675994 12.23803003226031 6 30.43347191353034 39.29776289357644 18.261754880224508 12.007010312668715 7 26.995413659473105 32.16031133512557 22.830786733798075 18.01348827160325 8 23.727800616508983 40.0352017330084 20.277647856851754 15.959349793630867 9 26.446119360322545 15.372527459001258 20.107333204774193 38.074019975902004 10 14.654859380076502 30.3390112785342 34.44962882674304 20.55650051464626 11 33.60131031188153 22.627311813280556 25.64812666348188 18.12325121135604 12 23.814021543887616 25.203782464607638 32.34476771801857 18.63742827348617 13 29.580438669523794 23.040386531453454 25.488419069976935 21.890755729045818 14 20.02950356159893 23.941730465842113 27.528462058556375 28.500303914002583 15 21.88550120520737 33.69421309376595 22.848302199947042 21.571983501079636 16 20.677516836234688 28.65080816656339 29.964812474237217 20.706862522964705 17 20.457774787034673 30.250426203641396 28.65972092363004 20.632078085693887 18 20.463920199392323 28.060292700504757 31.635104405249066 19.840682694853854 19 23.347510141240317 27.180298422716376 27.355142687114753 22.11704874892855 20 23.365262824572515 28.55446485117163 28.26920137144854 19.81107095280732 21 21.88164095928721 27.32761997573256 27.063380598878894 23.727358466101336 22 22.76809477663887 29.858462882334475 26.898603737016664 20.47483860400999 23 21.0730615420312 29.569246727721893 28.221982611544078 21.135709118702827 24 23.154416461480505 27.689076089033506 28.84498845316023 20.31151899632576 25 21.813367546098515 28.703918544501615 28.544501617372077 20.93821229202779 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 176.0 1 176.0 2 187.0 3 198.0 4 198.0 5 198.0 6 334.5 7 471.0 8 471.0 9 471.0 10 771.5 11 1072.0 12 1072.0 13 1072.0 14 1913.5 15 2755.0 16 2755.0 17 2755.0 18 5207.5 19 7660.0 20 7660.0 21 7660.0 22 13248.5 23 18837.0 24 18837.0 25 18837.0 26 28436.5 27 38036.0 28 38036.0 29 38036.0 30 51820.5 31 65605.0 32 65605.0 33 65605.0 34 73610.0 35 81615.0 36 81615.0 37 81615.0 38 86481.0 39 91347.0 40 91347.0 41 91347.0 42 94140.0 43 96933.0 44 96933.0 45 96933.0 46 97803.5 47 98674.0 48 98674.0 49 98674.0 50 91907.0 51 85140.0 52 85140.0 53 85140.0 54 72033.0 55 58926.0 56 58926.0 57 58926.0 58 49259.5 59 39593.0 60 39593.0 61 39593.0 62 31806.0 63 24019.0 64 24019.0 65 24019.0 66 18232.5 67 12446.0 68 12446.0 69 12446.0 70 9144.0 71 5842.0 72 5842.0 73 5842.0 74 3972.0 75 2102.0 76 2102.0 77 2102.0 78 1460.5 79 819.0 80 819.0 81 819.0 82 556.0 83 293.0 84 293.0 85 293.0 86 191.0 87 89.0 88 89.0 89 89.0 90 53.5 91 18.0 92 18.0 93 18.0 94 11.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08871661184435012 2 0.035759618928030354 3 0.03603259311832067 4 0.037124489879481894 5 0.026069035172724415 6 0.39417473077920484 7 0.18671434615857072 8 0.19872521053134426 9 0.20322928467113435 10 0.017470348178579714 11 0.002729741902903081 12 0.003548664473774005 13 0.0 14 0.07575033780556048 15 0.002183793522322464 16 0.0032756902834836965 17 0.005322996710661007 18 0.006005432186386777 19 0.0024567677126127725 20 0.0019108193320321563 21 1.36487095145154E-4 22 0.0 23 0.0 24 2.72974190290308E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 732670.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.35843890918774 #Duplication Level Percentage of deduplicated Percentage of total 1 91.65106159724029 72.73285172726797 2 6.099211145321698 9.680477501404981 3 1.052494872325988 2.5057305008314583 4 0.39035692564558844 1.2391246494649508 5 0.2124617737340347 0.8430317345705032 6 0.1125890979798629 0.5360937032325302 7 0.08829759745420741 0.49050116453784387 8 0.05750601266681726 0.3650869914504476 9 0.0435172165139088 0.3108112531395235 >10 0.24472564574221312 3.5270442537374316 >50 0.023370128062494777 1.3027563352929694 >100 0.020772755167303272 3.3696579249510528 >500 0.0019041692191131964 0.8949945515091758 >1k 0.0017310629264665422 2.2018377086092205 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2140 0.2920823836106296 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2135 0.29139994813490383 No Hit GTCCTACAGTGGACATTTCTAAATT 2120 0.28935264170772657 No Hit CTTTAGGACGTGAAATATGGCGAGG 2068 0.2822553127601785 No Hit GTCCTACAGTGTGCATTTCTCATTT 1608 0.21947124899340764 No Hit CTGTAGGACCTGGAATATGGCGAGA 1291 0.17620483983239385 No Hit CCCATGTACTCTGCGTTGATACCAC 1201 0.16392100126932999 No Hit CTGAAGGACCTGGAATATGGCGAGA 1188 0.16214666903244299 No Hit GTATCAACGCAGAGTACTTTTTTTT 1182 0.16132774646157205 No Hit GTACATGGGAAGCAGTGGTATCAAC 1095 0.14945336918394367 No Hit GGTATCAACGCAGAGTACTTTTTTT 742 0.10127342459770429 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.36487095145154E-4 0.0 7 0.0 0.0 0.0 1.36487095145154E-4 0.0 8 0.0 0.0 0.0 1.36487095145154E-4 0.0 9 0.0 0.0 0.0 1.36487095145154E-4 0.0 10 0.0 0.0 0.0 1.36487095145154E-4 0.0 11 0.0 0.0 0.0 1.36487095145154E-4 0.0 12 0.0 0.0 0.0 1.36487095145154E-4 1.36487095145154E-4 13 0.0 0.0 0.0 1.36487095145154E-4 1.36487095145154E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGCGT 45 1.611721E-6 19.06577 6 GCGCCAC 50 8.834673E-5 15.173447 13 TAGGACC 445 0.0 14.782342 4 AAAGGCG 60 2.4807356E-5 14.300309 5 GGACTAT 50 0.001459701 13.346039 6 CGCCACC 50 0.001518095 13.276766 14 TGCGCCA 65 5.534855E-5 13.130867 12 AGGACCT 820 0.0 12.905157 5 TTAGGCA 60 3.967652E-4 12.711386 4 TAAACCC 60 3.967652E-4 12.711386 4 TGGCGAA 60 4.1721232E-4 12.635909 18 GGCGAGG 495 0.0 12.635047 19 GGACCTG 795 0.0 12.590603 6 CTTAGGG 70 1.0534324E-4 12.258249 3 AGGCGTG 70 1.08908345E-4 12.214638 7 AATACGG 70 1.1073062E-4 12.192948 14 CCAACGA 110 3.9357474E-8 12.060726 19 TCCAACG 150 1.4551915E-11 12.004113 18 TGTAGGA 1265 0.0 11.908321 2 CTGTAGG 1285 0.0 11.8640375 1 >>END_MODULE