FastQCFastQC Report
Thu 26 May 2016
SRR1926677_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926677_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33733
Sequences flagged as poor quality0
Sequence length25
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC4071.2065336613998163No Hit
GTACATGGGAAGCAGTGGTATCAAC3781.120564432454866No Hit
GAGTACATGGGAAGCAGTGGTATCA2110.6255002519787745No Hit
GCGTTGATACCACTGCTTCCCATGT1260.3735214774849554No Hit
CATGTACTCTGCGTTGATACCACTG1250.3705570213144399No Hit
ACGCAGAGTACATGGGAAGCAGTGG1120.33201909109773814No Hit
CNCATGTACTCTGCGTTGATACCAC1120.33201909109773814No Hit
ACTCTGCGTTGATACCACTGCTTCC880.26087214300536565No Hit
GTATCAACGCAGAGTACATGGGAAG850.2519787744938191No Hit
GNACATGGGAAGCAGTGGTATCAAC800.2371564936412415No Hit
GGTATCAACGCAGAGTACATGGGAA740.21936975661814842No Hit
GTATCAACGCAGAGTACTTTTTTTT700.2075119319360863No Hit
TATCAACGCAGAGTACATGGGAAGC560.16600954554886907No Hit
GCTTCCCATGTACTCTGCGTTGATA550.16304508937835355No Hit
GTACTCTGCGTTGATACCACTGCTT550.16304508937835355No Hit
CTGCTTCCCATGTACTCTGCGTTGA510.15118726469629146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA500.14822280852577596No Hit
GGTATCAACGCAGAGTACTTTTTTT480.1422938961847449No Hit
CAGTGGTATCAACGCAGAGTACATG470.1393294400142294No Hit
GATACCACTGCTTCCCATGTACTCT470.1393294400142294No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA410.12154270299113627No Hit
GGGAAGCAGTGGTATCAACGCAGAG400.11857824682062075No Hit
ATACCACTGCTTCCCATGTACTCTG400.11857824682062075No Hit
GCAGAGTACATGGGAAGCAGTGGTA400.11857824682062075No Hit
GNGTACATGGGAAGCAGTGGTATCA380.11264933447958972No Hit
CCACTGCTTCCCATGTACTCTGCGT350.10375596596804315No Hit
GCAGTGGTATCAACGCAGAGTACAT340.10079150979752764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACA350.0053569919.272311
TGTACTC1102.3801105E-49.55019955
GTACTCT1301.2637486E-48.8890316
ATGTACT1100.0019198418.8730414
TACATGG3505.4569682E-118.7374912
CATGTAC1200.0035339028.2945063
GTACATG3754.5474735E-118.2742451
TACTCTG1250.00111826468.1821147
ACATGGG3602.1827873E-117.68009763
TGCGTTG1250.0037636817.240287312
ATGGGAA2405.4400134E-67.003485
CATGGGA2857.955041E-76.84936484
GAAGCAG2302.9369712E-66.78705179
GGAAGCA2308.877818E-66.7839578
TTGATAC1709.600342E-56.71636916
TGGGAAG2353.1333737E-56.7054596
TATCAAC2956.7429937E-96.60480519
TGGTATC2355.412603E-76.593882616
AACGCAG2404.203085E-56.56576256
TCTGCGT1400.0098115526.558915610
Produced by FastQC (version 0.11.5)