FastQCFastQC Report
Thu 26 May 2016
SRR1926677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33733
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC13053.8686153025227523No Hit
GTACATGGGAAGCAGTGGTATCAAC11083.2846174369311947No Hit
GAGTACATGGGAAGCAGTGGTATCA4511.3369697329024992No Hit
CATGTACTCTGCGTTGATACCACTG4211.2480360477870334No Hit
GTATCAACGCAGAGTACTTTTTTTT3541.0494174843624937No Hit
GCGTTGATACCACTGCTTCCCATGT3531.0464530281919782No Hit
ACGCAGAGTACATGGGAAGCAGTGG3120.9249103252008419No Hit
GTATCAACGCAGAGTACATGGGAAG2850.8448700085969229No Hit
ACTCTGCGTTGATACCACTGCTTCC2640.782616429016097No Hit
GGTATCAACGCAGAGTACTTTTTTT2560.7589007796519729No Hit
TATCAACGCAGAGTACATGGGAAGC2260.6699670945365073No Hit
GGTATCAACGCAGAGTACATGGGAA2190.6492159013428986No Hit
TATCAACGCAGAGTACTTTTTTTTT2030.6017846026146504No Hit
GTACTCTGCGTTGATACCACTGCTT1620.48024189962351405No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1610.47727744345299855No Hit
CAGTGGTATCAACGCAGAGTACATG1400.4150238638721726No Hit
GCTTCCCATGTACTCTGCGTTGATA1330.39427267067856403No Hit
CATGGGAAGCAGTGGTATCAACGCA1310.388343758337533No Hit
GCAGAGTACATGGGAAGCAGTGGTA1300.3853793021670175No Hit
ATACCACTGCTTCCCATGTACTCTG1230.3646281089734088No Hit
GGGAAGCAGTGGTATCAACGCAGAG1210.35869919663237776No Hit
CCACTGCTTCCCATGTACTCTGCGT1180.3498058281208312No Hit
GCAGTGGTATCAACGCAGAGTACAT1140.3379480034387692No Hit
GTGGTATCAACGCAGAGTACATGGG1100.3260901787567071No Hit
CTGCTTCCCATGTACTCTGCGTTGA1070.3171968102451605No Hit
GATACCACTGCTTCCCATGTACTCT980.2905167047105208No Hit
CTTCCCATGTACTCTGCGTTGATAC940.2786588800284588No Hit
GTTGATACCACTGCTTCCCATGTAC910.26976551151691225No Hit
ACATGGGAAGCAGTGGTATCAACGC880.26087214300536565No Hit
ATGGGAAGCAGTGGTATCAACGCAG870.25790768683485016No Hit
AAGCAGTGGTATCAACGCAGAGTAC860.25494323066433466No Hit
CCATGTACTCTGCGTTGATACCACT820.24308540598227255No Hit
TACCACTGCTTCCCATGTACTCTGC800.2371564936412415No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA700.2075119319360863No Hit
ATCAACGCAGAGTACATGGGAAGCA640.18972519491299322No Hit
ATCAACGCAGAGTACTTTTTTTTTT590.1749029140604156No Hit
GTACTGGTTCACTATCGGTCAGTCA580.17193845788990011No Hit
GAGTACTTTTTTTTTTTTTTTTTTT570.1689740017193846No Hit
GTACATGGTAAGCAGTGGTATCAAC560.16600954554886907No Hit
GCAGAGTACTTTTTTTTTTTTTTTT540.16008063320783802No Hit
AAAAAGTACTCTGCGTTGATACCAC490.1452583523552604No Hit
GTACTTTTTTTTTTTTTTTTTTTTT450.13340052767319835No Hit
GGATACCACGTGTCCCGCCCTACTC420.12450715916165178No Hit
ACCATGTACTCTGCGTTGATACCAC410.12154270299113627No Hit
GGTATCAACGCAGAGTACATGGGGG390.11561379065010524No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA370.10968487830907421No Hit
GGAAGCAGTGGTATCAACGCAGAGT370.10968487830907421No Hit
CTTATACACATCTCCGAGCCCACGA360.10672042213855867No Hit
CTATCGGTCAGTCAGGAGTATTTAG340.10079150979752764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGTA3050.08.4588692
GCTTCCC1605.9552913E-68.28653615
TACTCTG3500.08.14158257
CTTCCCA1659.004014E-68.03542916
CTGCGTT3550.08.00309311
CTCTGCG3650.07.7953969
TCTGCGT3550.07.736323410
GTACTCT3600.07.6973976
TGCGTTG3600.07.628874312
CCCATGT3403.6379788E-127.5881041
ATGTACT3403.6379788E-127.5881044
TGTACTC3403.6379788E-127.5881045
CATGTAC3403.6379788E-127.5881043
TCCCATG1751.9765374E-57.57626118
TTCCCAT1751.9765374E-57.57626117
ACTCTGC3850.07.39044058
TGCTTCC1802.8741235E-57.365809414
CTGCTTC1802.8741235E-57.365809413
TTGATAC3801.8189894E-127.227354516
TGATACC3950.07.19265317
Produced by FastQC (version 0.11.5)