FastQCFastQC Report
Thu 26 May 2016
SRR1926676_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926676_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707435
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12460.17612925569133558No Hit
GTCCTAAAGTGTGTATTTCTCATTT10090.14262794461681993No Hit
CTGTAGGACGTGGAATATGGCAAGA8920.12608932269395776No Hit
CTTTAGGACGTGAAATATGGCGAGG8050.11379137305900895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA450.001149358117.9031371
TCCTATA851.1518314E-616.1972452
TAATACC505.793482E-415.0787434
CCCCTAG550.00438914314.6480211
GTATAGC657.9538266E-414.4602261
TAGGACC3450.014.0485194
CTAAGAC550.001029389113.9766233
TGTAGGA7300.013.9562562
TACAGGA700.001152721413.7676572
CCCTACA600.00662066813.7676562
CTGTAGG7400.013.6088041
GTTCTAA700.001392435613.4273531
GTCCTAT904.4782E-513.4273531
GTATAAG700.001392435613.4273531
ATAAGAC500.00884780113.1779583
TAGCCCT901.8258215E-613.1639834
TTTAGGA4700.012.8888712
GTCCTAG957.4355E-512.7206491
GTGTAGA957.4355E-512.7206491
GTCCTAA4450.012.6730071
Produced by FastQC (version 0.11.5)