##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926676_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 707435 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.53443779287143 26.0 2.0 30.0 2.0 31.0 2 15.30931746379526 10.0 2.0 30.0 2.0 31.0 3 18.61835221610466 16.0 7.0 30.0 2.0 31.0 4 20.63497989214557 27.0 2.0 33.0 2.0 35.0 5 20.79193706842325 27.0 2.0 35.0 2.0 35.0 6 21.683754691243717 29.0 2.0 35.0 2.0 36.0 7 21.567934863273656 28.0 2.0 35.0 2.0 37.0 8 21.719285870786717 28.0 2.0 35.0 2.0 37.0 9 24.31663827772163 30.0 8.0 37.0 2.0 39.0 10 24.96685066472538 31.0 2.0 37.0 2.0 39.0 11 22.241957211616615 28.0 2.0 35.0 2.0 39.0 12 23.292522987977694 30.0 2.0 37.0 2.0 39.0 13 20.075315753390772 25.0 2.0 35.0 2.0 39.0 14 23.222980203128202 29.0 2.0 37.0 2.0 40.0 15 24.27444076134203 30.0 2.0 37.0 2.0 40.0 16 24.62493091238064 31.0 2.0 38.0 2.0 40.0 17 23.963993865160756 30.0 2.0 37.0 2.0 40.0 18 24.03145872058917 30.0 2.0 37.0 2.0 40.0 19 24.38204923420526 30.0 2.0 38.0 2.0 40.0 20 24.62229321421756 31.0 2.0 38.0 2.0 40.0 21 22.57736470488455 30.0 2.0 36.0 2.0 40.0 22 23.446630432477896 30.0 2.0 37.0 2.0 40.0 23 23.409311102786827 30.0 2.0 37.0 2.0 40.0 24 22.533352180765725 30.0 2.0 38.0 2.0 40.0 25 22.321269091860028 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.918782596568384 1101 2 9.465771954833897 1101 3 6.269432928575238 1101 4 7.510908614460348 1101 5 7.238378426618418 1101 6 7.067911098439083 1101 7 6.755496105828822 1101 8 5.450209988920815 1101 9 5.213174693249993 1101 10 4.809265789128148 1101 11 7.634594687405443 1101 12 5.436195041181332 1101 13 9.83191331684175 1101 14 7.178721977503457 1101 15 6.5344966594641605 1101 16 5.8872935655086245 1101 17 2.0149012964682065 1101 18 4.364078347660108 1101 19 4.95455111273845 1101 20 3.3157778683332673 1101 21 0.8341565973080769 1101 22 4.179250175089823 1101 23 5.129905668836976 1101 24 6.512954066804099 1101 25 7.251144060240627 1102 1 5.031489893417472 1102 2 9.892036465613334 1102 3 6.318483509006416 1102 4 7.408039626068973 1102 5 7.344319554313277 1102 6 6.220203801589996 1102 7 6.424418162213566 1102 8 6.527108828058459 1102 9 5.622030414643028 1102 10 4.800497132411728 1102 11 7.391240624387201 1102 12 5.028326052789954 1102 13 9.384682802745562 1102 14 6.941553652461998 1102 15 5.066959346031322 1102 16 5.6436824543975135 1102 17 6.276182390995569 1102 18 6.245927435553973 1102 19 5.158427563816396 1102 20 5.612017503490815 1102 21 4.632460909082539 1102 22 5.50379826795881 1102 23 5.849445469831998 1102 24 7.095972308926818 1102 25 6.745277559411438 1103 1 4.244858755508783 1103 2 8.330369085076722 1103 3 5.858180169193343 1103 4 7.303612808720835 1103 5 6.648715071210031 1103 6 6.229517058703983 1103 7 6.5683769005308115 1103 8 6.183918598192339 1103 9 5.199742022168316 1103 10 4.548145480077373 1103 11 5.139302192924205 1103 12 4.128645372307158 1103 13 7.86076541397199 1103 14 6.353363257856657 1103 15 5.36909930847078 1103 16 5.258932638356306 1103 17 3.0561319807949197 1103 18 1.9049282372848317 1103 19 3.8467972496037923 1103 20 4.406854027273667 1103 21 6.716336509007856 1103 22 2.259443331822716 1103 23 4.527549156695695 1103 24 6.127048989541404 1103 25 2.3261164664216416 1104 1 5.160367563888645 1104 2 9.672716399278343 1104 3 6.678436196549097 1104 4 7.076889006617211 1104 5 6.88140130243541 1104 6 6.447535281445617 1104 7 6.605888262691568 1104 8 6.32762828957441 1104 9 5.357226980831694 1104 10 4.322909579977821 1104 11 7.1959182168172084 1104 12 4.262534910462378 1104 13 8.298874101155473 1104 14 6.383705637634176 1104 15 5.659169862078539 1104 16 5.374891604724311 1104 17 5.591224825879973 1104 18 3.4801240992940947 1104 19 4.093031504895315 1104 20 3.28641512323523 1104 21 2.5800879698570967 1104 22 4.631554096658451 1104 23 5.0417193943271705 1104 24 5.911222040660306 1104 25 5.176552556972652 1105 1 2.5030039080437945 1105 2 8.271987801828434 1105 3 5.410840032400376 1105 4 5.037520636318273 1105 5 1.603310120607489 1105 6 4.041312737783347 1105 7 4.836971515664889 1105 8 2.328734579084749 1105 9 2.9442675894248573 1105 10 2.728459778199184 1105 11 5.848296873197793 1105 12 3.3810311067551027 1105 13 4.030383262196938 1105 14 2.656522523951544 1105 15 3.1029392824149795 1105 16 3.3384001228856732 1105 17 3.8927838101294086 1105 18 1.7430674186983595 1105 19 1.5566685990772484 1105 20 0.9219663929234336 1105 21 2.149880641023927 1105 22 3.282269300772885 1105 23 1.1535805322249537 1105 24 3.408090678825957 1105 25 4.141649524721501 1106 1 6.322012933647038 1106 2 10.637715507535876 1106 3 7.4613978830963745 1106 4 8.863134403652431 1106 5 8.513818501524785 1106 6 7.579761018182261 1106 7 7.331354853822404 1106 8 7.000242091649547 1106 9 6.069472178536245 1106 10 5.468381626474322 1106 11 7.743702499069435 1106 12 5.013650899929324 1106 13 4.525257351084658 1106 14 5.546144395909021 1106 15 5.6710536418076565 1106 16 5.610331286214883 1106 17 6.158672029480513 1106 18 6.264755180190871 1106 19 6.055708148586497 1106 20 5.700500982297957 1106 21 5.44099581614168 1106 22 5.972004321692285 1106 23 6.501118884460304 1106 24 7.310107624856531 1106 25 6.286263107859675 1107 1 3.8271865513706445 1107 2 -12.855061378499435 1107 3 -5.0207143676313795 1107 4 1.813257282741219 1107 5 4.300503123954979 1107 6 4.8793094035238305 1107 7 4.550233797515666 1107 8 5.107441627338893 1107 9 3.3996315132661366 1107 10 3.0935777342451765 1107 11 5.437538593781554 1107 12 2.965424258291904 1107 13 4.374818885849013 1107 14 3.762933034807734 1107 15 3.471118935493216 1107 16 3.3924045421026534 1107 17 3.8685594885586028 1107 18 -1.1001549049629773 1107 19 1.5366233484123804 1107 20 1.8030438086076828 1107 21 -0.9314547828372 1107 22 1.83028528404866 1107 23 -0.8911011915342932 1107 24 2.465157456634607 1107 25 3.121210646197042 1108 1 5.468142328640884 1108 2 10.327759740491896 1108 3 0.6613025109235444 1108 4 6.142358575059244 1108 5 7.382426889975392 1108 6 7.693322839967845 1108 7 7.695827075095963 1108 8 7.458886111852255 1108 9 6.05668927165819 1108 10 5.1421164589469015 1108 11 7.3023823154590275 1108 12 3.0589412980765225 1108 13 3.593820330238124 1108 14 5.365256650474301 1108 15 5.408590434089849 1108 16 5.418308143916821 1108 17 0.6076574036392088 1108 18 4.028668576186508 1108 19 5.009367897608428 1108 20 5.313451233187486 1108 21 4.873701514565763 1108 22 2.239657976508262 1108 23 4.846846173171095 1108 24 5.598359898167374 1108 25 6.130291026355678 1109 1 3.5911262212676647 1109 2 -12.855061378499435 1109 3 -4.90962547826785 1109 4 2.45813930144244 1109 5 0.9101179347713177 1109 6 2.481337321374127 1109 7 3.7207246543452612 1109 8 3.854083284830999 1109 9 3.555430774511688 1109 10 2.870075064722908 1109 11 1.1386317095920226 1109 12 1.4620130042927997 1109 13 2.7553402080473077 1109 14 3.4087172996098474 1109 15 3.3505952961923064 1109 16 3.2768464925334833 1109 17 3.8132085672514187 1109 18 3.9856995719316934 1109 19 2.673894202821316 1109 20 1.5601583815650244 1109 21 1.1654162872305598 1109 22 3.21173280005641 1109 23 3.685098799016071 1109 24 4.42843655811658 1109 25 4.564359109692646 1110 1 3.265362300759019 1110 2 8.887748810489882 1110 3 6.088610551144399 1110 4 7.589377527087027 1110 5 7.875875687642793 1110 6 3.9694866941662816 1110 7 6.336465703199405 1110 8 6.953028394837002 1110 9 5.182273570269988 1110 10 5.055530511122122 1110 11 3.0182916197527447 1110 12 5.436725032964404 1110 13 9.823132160994035 1110 14 7.560750873696282 1110 15 6.623999535388563 1110 16 6.304505973068196 1110 17 6.622102748125286 1110 18 6.700653258643399 1110 19 5.617366094935111 1110 20 5.85843809840722 1110 21 3.5295599114685814 1110 22 5.81810049007202 1110 23 6.373538235257133 1110 24 7.201538016406946 1110 25 7.3234153557760955 1111 1 5.027000377971323 1111 2 9.037271411060107 1111 3 7.446979149347399 1111 4 7.901941784661545 1111 5 8.317482559299222 1111 6 7.4163735780475655 1111 7 7.439117639270911 1111 8 7.52161292529928 1111 9 5.958699434957442 1111 10 5.031812009900307 1111 11 6.566268151244486 1111 12 6.191341860104657 1111 13 7.946736590359965 1111 14 5.725126273316398 1111 15 5.20223727936628 1111 16 5.376327007596714 1111 17 6.519078838610675 1111 18 6.218790147551353 1111 19 4.927192493923879 1111 20 5.078269711726417 1111 21 7.6146935739258055 1111 22 6.981116678624353 1111 23 6.975458958668405 1111 24 6.887494577951461 1111 25 7.451259612171022 1112 1 -0.8189389989191262 1112 2 7.7710465989381134 1112 3 6.311785198250234 1112 4 -0.006209301199064043 1112 5 4.567060269218473 1112 6 3.749520687480178 1112 7 6.459702391082651 1112 8 6.597583075141603 1112 9 5.272573027926331 1112 10 3.8313481152092628 1112 11 0.46861496675005654 1112 12 4.55471639492831 1112 13 8.212955487134522 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2204 5 0.37108675995175133 2204 6 3.0197532037022405 2204 7 4.159213211091981 2204 8 3.833959988920814 2204 9 2.5968479388640304 2204 10 2.2049346487772716 2204 11 3.6710859154756186 2204 12 3.660668725391858 2204 13 2.135948404561045 2204 14 2.982943468731527 2204 15 3.062786133148368 2204 16 3.1067672497191516 2204 17 -1.7123903701984595 2204 18 1.6968853652039684 2204 19 2.3531256741419604 2204 20 2.814166026228005 2204 21 4.108290369237903 2204 22 3.4142392101775414 2204 23 2.4142039144510115 2204 24 3.976484768558482 2204 25 4.152569498837117 2205 1 5.257312707503409 2205 2 9.585509144479964 2205 3 6.51396673734439 2205 4 7.068790283768326 2205 5 6.872745832851908 2205 6 6.673461811592809 2205 7 6.722564251629141 2205 8 6.599956423152193 2205 9 5.25635483059812 2205 10 4.81670768030617 2205 11 6.905847989043053 2205 12 6.084744961942029 2205 13 8.992462709340426 2205 14 6.629388908301134 2205 15 5.663300145993478 2205 16 5.108200855524473 2205 17 5.885544455475074 2205 18 5.838671585906276 2205 19 4.646079110625404 2205 20 4.85730322491013 2205 21 -1.1321917914927653 2205 22 3.0940824518991192 2205 23 4.477162668308711 2205 24 6.275954595066114 2205 25 6.732040785016114 2206 1 3.6496024346250664 2206 2 7.847772629597731 2206 3 5.302958569600882 2206 4 1.3338370894940859 2206 5 4.87168139557928 2206 6 5.371726887912768 2206 7 5.440678292063641 2206 8 5.393651284467371 2206 9 4.071987108904516 2206 10 3.211678069829901 2206 11 2.7499826766092212 2206 12 3.9284824905740443 2206 13 7.100928161646067 2206 14 -0.30965521547899755 2206 15 1.968808400354849 2206 16 2.5418380999215806 2206 17 3.435370257331904 2206 18 -1.5078717198162757 2206 19 0.26084328547799274 2206 20 1.5831564108433902 2206 21 2.540691685027376 2206 22 -2.4525750610775177 2206 23 -3.2994094458728753 2206 24 0.21122667139249174 2206 25 1.6428275960031087 2207 1 1.7194962996678953 2207 2 -12.855061378499435 2207 3 -5.582384635318181 2207 4 -3.853360009791963 2207 5 1.2057370454329899 2207 6 1.897613382289002 2207 7 3.054125795877763 2207 8 3.119492207517876 2207 9 0.9089441331629899 2207 10 0.608484114304872 2207 11 -1.2901755681682374 2207 12 -2.17570544821508 2207 13 0.4924135887286418 2207 14 -4.862149419995184 2207 15 -1.36301557544612 2207 16 -0.33819175259904455 2207 17 0.9579042872240535 2207 18 0.8925258734893617 2207 19 0.49489233079174255 2207 20 0.45184230552739635 2207 21 -3.0427289383091036 2207 22 -3.5523254094670023 2207 23 -0.6795165715110407 2207 24 0.9780329140036628 2207 25 1.4977522712248579 2208 1 4.903962410953536 2208 2 6.432641637741863 2208 3 6.244608875984365 2208 4 7.918542025133203 2208 5 3.6203254490468773 2208 6 6.046493851726012 2208 7 6.48647831773911 2208 8 6.441679439810219 2208 9 5.147235018075978 2208 10 4.5394185346811255 2208 11 6.6272338985423325 2208 12 6.121968436386279 2208 13 8.688386634707179 2208 14 3.1119102992358627 2208 15 4.596232931699273 2208 16 5.156185676877534 2208 17 5.879269045888162 2208 18 0.9869747127026436 2208 19 3.3111346394205867 2208 20 4.1464874584338105 2208 21 6.855968275575673 2208 22 5.807461698678377 2208 23 1.4058975481873226 2208 24 3.0457150876857675 2208 25 4.283887679730185 2209 1 2.6661536682847427 2209 2 4.730756510299919 2209 3 4.77995602822606 2209 4 0.6013652354649146 2209 5 4.450219012167707 2209 6 4.801092000937068 2209 7 5.583795864088291 2209 8 4.099597579573512 2209 9 3.883370771681367 2209 10 3.015876721172198 2209 11 -0.9085002629034484 2209 12 2.7803408913022025 2209 13 1.4998209182384699 2209 14 3.2873675590237355 2209 15 3.659818980173263 2209 16 3.1020941295698634 2209 17 1.0820924057886465 2209 18 2.855336741428573 2209 19 3.4719933636114035 2209 20 3.6272021229666294 2209 21 5.707908276061772 2209 22 4.466196857872525 2209 23 0.2512278552457836 2209 24 -3.023317756993915 2209 25 -0.19716039852918144 2210 1 -2.0928997419434694 2210 2 -12.855061378499435 2210 3 -7.302035455180553 2210 4 -2.2908602598923373 2210 5 -0.9729059833025424 2210 6 -1.428505500089127 2210 7 -1.5725823896740394 2210 8 -19.259790011079186 2210 9 -12.304835663795522 2210 10 -7.059802758159943 2210 11 -9.011787841894094 2210 12 -4.613064975171898 2210 13 -5.327568830882427 2210 14 -4.089549349833693 2210 15 -5.803953872015807 2210 16 -5.335966859675349 2210 17 -4.791256375922089 2210 18 -4.949804181639429 2210 19 -9.545431852647212 2210 20 -6.901979695035497 2210 21 -5.151893416319613 2210 22 -9.098030419077524 2210 23 -7.361291523858583 2210 24 -9.805830343116861 2210 25 -7.582151796941167 2211 1 -0.03706715712619513 2211 2 4.389602004094982 2211 3 0.44304951314009955 2211 4 1.4754570545260286 2211 5 0.8708045349928035 2211 6 -0.0018795419550059478 2211 7 -19.124503894171177 2211 8 -12.444519075118595 2211 9 -8.633097884746721 2211 10 -6.942560976060687 2211 11 -9.067749319162703 2211 12 -4.495389523679098 2211 13 -6.052388817805214 2211 14 -4.569287053695231 2211 15 -4.973344063031735 2211 16 -10.594960129072657 2211 17 -7.259881133745257 2211 18 -5.493450387972075 2211 19 -8.348712434059578 2211 20 -6.37322459472092 2211 21 -4.198466555401282 2211 22 -5.703167723103938 2211 23 -7.722889897665489 2211 24 -20.13371259986257 2211 25 -19.92802260642604 2212 1 -1.4458798041377037 2212 2 -12.855061378499435 2212 3 -7.518061187341207 2212 4 -18.165758052206318 2212 5 -18.30078824004825 2212 6 -19.154588901560917 2212 7 -9.466074944480091 2212 8 -9.107098042853238 2212 9 -5.523565524461098 2212 10 -5.936758776920691 2212 11 -5.717789101944366 2212 12 -3.2477369976536217 2212 13 -5.968304394249749 2212 14 -3.969310532058177 2212 15 -4.049064682106891 2212 16 -4.171066981711146 2212 17 -4.217672254576218 2212 18 -3.377782493763842 2212 19 -3.891524939041485 2212 20 -4.063121072531693 2212 21 -1.93041901316559 2212 22 -3.0256828934597486 2212 23 -3.252450171115953 2212 24 -2.2378608787681316 2212 25 -2.5935124563819087 2213 1 -17.093717403431615 2213 2 -12.855061378499435 2213 3 -8.098466708118675 2213 4 -5.460944246575073 2213 5 -18.30078824004825 2213 6 -10.05013840201505 2213 7 -19.124503894171177 2213 8 -9.938264125520604 2213 9 -7.779395697304047 2213 10 -6.90146309370564 2213 11 -5.983023387072304 2213 12 -4.85519006326917 2213 13 -17.561420016491585 2213 14 -11.500285742750856 2213 15 -6.665298980862815 2213 16 -6.074452574364219 2213 17 -4.6342653701984595 2213 18 -4.698514749524271 2213 19 -5.4486618754541 2213 20 -5.428429670268002 2213 21 -3.58723986045759 2213 22 -4.428999143711266 2213 23 -4.250314585477284 2213 24 -3.299925133922514 2213 25 -4.267713796253471 2214 1 -17.093717403431615 2214 2 -12.855061378499435 2214 3 -11.59787099299339 2214 4 -18.165758052206318 2214 5 -18.30078824004825 2214 6 -19.154588901560917 2214 7 -15.428425462798629 2214 8 -14.034299815000754 2214 9 -13.30094295380883 2214 10 -13.292449897146362 2214 11 -13.364866096908283 2214 12 -11.945471625485336 2214 13 -17.561420016491585 2214 14 -15.666621159751443 2214 15 -21.94050334053584 2214 16 -17.08246133645216 2214 17 -21.63426537019846 2214 18 -16.5901373386144 2214 19 -14.08000771079096 2214 20 -12.999859386568692 2214 21 -10.627264971319372 2214 22 -11.652269432753315 2214 23 -11.546515899790476 2214 24 -10.51214397241159 2214 25 -10.300571626033884 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 161576.0 3 4.0 4 814.0 5 2884.0 6 3692.0 7 3407.0 8 3053.0 9 2905.0 10 2738.0 11 2560.0 12 2358.0 13 2476.0 14 3775.0 15 7344.0 16 10407.0 17 11175.0 18 11662.0 19 13648.0 20 16281.0 21 18863.0 22 19554.0 23 20881.0 24 21808.0 25 21886.0 26 18307.0 27 15152.0 28 16264.0 29 18584.0 30 17685.0 31 20252.0 32 25550.0 33 31349.0 34 37039.0 35 40739.0 36 38882.0 37 38336.0 38 23545.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.26507645698978 19.243538311697847 13.547235622610973 25.9441496087014 2 12.60667036821485 20.30522163577212 43.446810313494375 23.64129768251866 3 18.741585740185606 25.632303577518893 29.359110137799398 26.2670005444961 4 11.008241420312435 17.557095411866005 39.19176678010579 32.242896387715774 5 11.535692311720274 37.968326591524956 36.658746678186645 13.837234418568118 6 29.42979003278558 38.264290957890296 19.863011423465437 12.442907585858688 7 25.952275802230623 31.059117643845962 24.685591761638058 18.303014792285353 8 23.970632630512704 39.1892865188082 21.0056990840703 15.834381766608793 9 26.400032830405486 15.344676804469465 20.848298054798477 37.40699231032657 10 14.384263017344987 29.70318187424526 35.606414805338595 20.306140303071153 11 32.57955509282736 21.705043687425114 26.434893694680174 19.280507525067353 12 24.49714584121153 24.524306868588646 32.75890011344606 18.21964717675376 13 28.71002551679532 21.98193390626504 25.191882585589344 24.116157991350295 14 19.14063902673743 24.069145307124952 28.43762343528939 28.352592230848227 15 21.24118398643343 34.386860005856704 23.401025360107113 20.97093064760276 16 19.850455638425384 28.16671254363774 31.072690968353626 20.910140849583247 17 19.817507882989492 29.3334595582613 30.690426081953966 20.158606476795246 18 19.721715917734688 27.263659457209695 33.81374054566069 19.20088407939492 19 22.653360088009304 26.467479155879452 27.828209736388033 23.050951019723207 20 22.901552200722943 28.510596073428307 29.192005103125663 19.395846622723084 21 20.887606639479568 26.338954822363508 27.02921382821336 25.74422470994357 22 21.937479526685706 30.07522685868959 28.35949128483337 19.62780232979133 23 20.2828019240267 29.401692226484943 29.737505359849315 20.578000489639038 24 22.293724006914374 27.493508049396393 30.117671953061915 20.095095990627318 25 21.373965860299506 28.2476161364003 29.92516550480735 20.453252498492837 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 813.0 1 813.0 2 1638.5 3 2464.0 4 2464.0 5 2464.0 6 3561.0 7 4658.0 8 4658.0 9 4658.0 10 5391.0 11 6124.0 12 6124.0 13 6124.0 14 7720.5 15 9317.0 16 9317.0 17 9317.0 18 13494.0 19 17671.0 20 17671.0 21 17671.0 22 24913.5 23 32156.0 24 32156.0 25 32156.0 26 42192.0 27 52228.0 28 52228.0 29 52228.0 30 63253.0 31 74278.0 32 74278.0 33 74278.0 34 80293.5 35 86309.0 36 86309.0 37 86309.0 38 87619.5 39 88930.0 40 88930.0 41 88930.0 42 88187.5 43 87445.0 44 87445.0 45 87445.0 46 84096.5 47 80748.0 48 80748.0 49 80748.0 50 72538.0 51 64328.0 52 64328.0 53 64328.0 54 53591.5 55 42855.0 56 42855.0 57 42855.0 58 35131.5 59 27408.0 60 27408.0 61 27408.0 62 21582.0 63 15756.0 64 15756.0 65 15756.0 66 11884.0 67 8012.0 68 8012.0 69 8012.0 70 5846.5 71 3681.0 72 3681.0 73 3681.0 74 2561.0 75 1441.0 76 1441.0 77 1441.0 78 991.0 79 541.0 80 541.0 81 541.0 82 358.0 83 175.0 84 175.0 85 175.0 86 122.5 87 70.0 88 70.0 89 70.0 90 44.5 91 19.0 92 19.0 93 19.0 94 12.5 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.354725169096806 2 36.6074621696693 3 5.502555005053468 4 13.811869641733871 5 13.615809226289342 6 14.890555316036103 7 9.41825043996975 8 7.756755037565288 9 0.10926798928523468 10 1.4380119728314262 11 5.503827206739842 12 5.801098334122569 13 16.294924622050083 14 1.5860114356796031 15 2.974407542742443 16 2.9424611448401623 17 2.9444401252411883 18 1.5075589983532056 19 1.4469173846360444 20 0.2805911497169351 21 4.262299716581736 22 1.6124449596075965 23 0.11195374840091314 24 9.147978259486738 25 0.11435679603073075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 707435.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.07168216109342 #Duplication Level Percentage of deduplicated Percentage of total 1 97.10675527695768 88.43675552278171 2 1.9716621004590258 3.591251682841565 3 0.3937683665667383 1.0758344257517674 4 0.15516273006192147 0.5652372334178739 5 0.09424365295017695 0.4291464003589454 6 0.048873571055393604 0.26705989975406524 7 0.03906625041472307 0.24904803986967403 8 0.03023340263089333 0.2202725468039266 9 0.01856103243051324 0.1521346001484104 >10 0.12191611827726842 2.157004858544205 >50 0.010922981279846202 0.7108208053420831 >100 0.008031379015921337 1.4652029725376932 >500 4.818827399553617E-4 0.35035554266151664 >1k 3.212551599702411E-4 0.329875469186522 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1246 0.17612925569133558 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1009 0.14262794461681993 No Hit CTGTAGGACGTGGAATATGGCAAGA 892 0.12608932269395776 No Hit CTTTAGGACGTGAAATATGGCGAGG 805 0.11379137305900895 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.413557429304459E-4 0.0 10 0.0 0.0 0.0 1.413557429304459E-4 0.0 11 0.0 0.0 0.0 1.413557429304459E-4 0.0 12 0.0 0.0 0.0 1.413557429304459E-4 0.0 13 0.0 0.0 0.0 1.413557429304459E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGA 45 0.0011493581 17.903137 1 TCCTATA 85 1.1518314E-6 16.197245 2 TAATACC 50 5.793482E-4 15.078743 4 CCCCTAG 55 0.004389143 14.648021 1 GTATAGC 65 7.9538266E-4 14.460226 1 TAGGACC 345 0.0 14.048519 4 CTAAGAC 55 0.0010293891 13.976623 3 TGTAGGA 730 0.0 13.956256 2 TACAGGA 70 0.0011527214 13.767657 2 CCCTACA 60 0.006620668 13.767656 2 CTGTAGG 740 0.0 13.608804 1 GTTCTAA 70 0.0013924356 13.427353 1 GTCCTAT 90 4.4782E-5 13.427353 1 GTATAAG 70 0.0013924356 13.427353 1 ATAAGAC 50 0.008847801 13.177958 3 TAGCCCT 90 1.8258215E-6 13.163983 4 TTTAGGA 470 0.0 12.888871 2 GTCCTAG 95 7.4355E-5 12.720649 1 GTGTAGA 95 7.4355E-5 12.720649 1 GTCCTAA 445 0.0 12.673007 1 >>END_MODULE