FastQCFastQC Report
Thu 26 May 2016
SRR1926676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707435
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA27570.3897177832592394No Hit
GTCCTACAGTGGACATTTCTAAATT27130.38349813057029974No Hit
CTTTAGGACGTGAAATATGGCGAGG26660.3768544106525688No Hit
GTCCTAAAGTGTGTATTTCTCATTT25020.35367206881197566No Hit
GTCCTACAGTGTGCATTTCTCATTT16440.2323888413776531No Hit
CTGTAGGACCTGGAATATGGCGAGA14420.20383498130570302No Hit
CTGAAGGACCTGGAATATGGCGAGA14340.20270413536225942No Hit
GTATCAACGCAGAGTACTTTTTTTT12830.1813594181797621No Hit
CCCATGTACTCTGCGTTGATACCAC10460.1478581071052464No Hit
GTACATGGGAAGCAGTGGTATCAAC9350.13216761963996693No Hit
GAATATGGCAAGAAAACTGAAAATC8580.1212832274343226No Hit
GTCCTTCAGTGTGCATTTCTCATTT8430.1191628912903659No Hit
CTGTAGGACATGGAATATGGCAAGA7440.10516867274025175No Hit
GGTATCAACGCAGAGTACTTTTTTT7250.10248291362457329No Hit
GGAATATGGCGAGAAAACTGAAAAT7180.10149342342406016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACT500.001453189113.3538494
TAGGACC5050.013.2216334
CCAACGA802.0579755E-613.02639619
GTCCTAA6700.012.5254571
TCCAACG1155.524271E-912.35705518
GTAAGGG550.002969424712.1424563
CTCTTAC959.902215E-712.0460151
GGCGAGG7900.011.99207919
AGGACCT9250.011.9617495
TCATATA802.7392232E-511.9256272
CAGGACC802.7452184E-511.923084
TTAGAAC657.7328354E-411.7421553
TTAATCC1302.4429028E-911.7396484
TAGGACA5450.011.7261854
CTAGGAC1156.644041E-811.6145233
TGTAGGA14750.011.5132822
GGACCTG9400.011.364176
CTGTAGG14500.011.3121771
TTAGTAC855.087671E-511.2241183
ATGTCGA855.4650307E-511.14557817
Produced by FastQC (version 0.11.5)