FastQCFastQC Report
Thu 26 May 2016
SRR1926675_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926675_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807149
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13100.16229964975487798No Hit
GTCCTAAAGTGTGTATTTCTCATTT11480.14222900604473276No Hit
CTGTAGGACGTGGAATATGGCAAGA10320.12785743400536953No Hit
CTTTAGGACGTGAAATATGGCGAGG8430.10444168301020011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC452.2681133E-417.0557753
TGTAGGA7900.015.9916442
CTGTAGG8250.015.259571
CTAGGAC1153.8198777E-1115.2548543
AGGACGG702.7762617E-614.88802055
GTATTAT550.00446646414.6102291
GTTCCTA658.114182E-414.4229171
GTCCTAC9200.014.4117191
TGTAGAA2400.014.3041192
GACCTAC852.6812095E-514.1805141
TACTGGA700.001176022113.7319552
CATAATA600.00673752713.7319552
GTCCTAA5700.013.6276681
TCCTACA9750.013.5206942
GTAGGAC8750.013.40792753
TAGGACC2650.013.39135554
CTGTAGA1953.6379788E-1213.0493061
ACCTACA956.029367E-513.009222
ACCAATC450.00964827613.0070338
TCCTAAA6450.012.986812
Produced by FastQC (version 0.11.5)