##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926675_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 807149 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.13729683119226 26.0 2.0 30.0 2.0 31.0 2 15.059147691442348 10.0 2.0 30.0 2.0 31.0 3 18.272943409457238 16.0 2.0 30.0 2.0 31.0 4 20.249629250609242 26.0 2.0 33.0 2.0 35.0 5 20.375991297765346 26.0 2.0 33.0 2.0 35.0 6 21.283580850623615 28.0 2.0 35.0 2.0 35.0 7 21.16643271564482 28.0 2.0 35.0 2.0 36.0 8 21.295690138995404 28.0 2.0 35.0 2.0 37.0 9 23.822918692831188 30.0 2.0 37.0 2.0 39.0 10 24.4448212164049 31.0 2.0 37.0 2.0 39.0 11 21.778785577384102 28.0 2.0 35.0 2.0 39.0 12 22.796896236010948 30.0 2.0 35.0 2.0 39.0 13 19.68693017026596 23.0 2.0 35.0 2.0 39.0 14 22.722030257114856 28.0 2.0 36.0 2.0 40.0 15 23.729269317065373 30.0 2.0 37.0 2.0 40.0 16 24.068914165785994 30.0 2.0 38.0 2.0 40.0 17 23.413413136855773 30.0 2.0 37.0 2.0 40.0 18 23.466872906984957 30.0 2.0 37.0 2.0 40.0 19 23.791184775053924 30.0 2.0 37.0 2.0 40.0 20 24.025282816431663 30.0 2.0 38.0 2.0 40.0 21 21.990216180655615 29.0 2.0 36.0 2.0 40.0 22 22.8539761555797 30.0 2.0 37.0 2.0 40.0 23 22.81607113432588 30.0 2.0 37.0 2.0 40.0 24 21.979669181278798 30.0 2.0 37.0 2.0 40.0 25 21.765117716803218 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.810053242877586 1101 2 9.094787234783416 1101 3 5.953068973788454 1101 4 7.574571132697571 1101 5 6.673838929031323 1101 6 6.627920216743636 1101 7 6.373192256538669 1101 8 4.784201932303116 1101 9 4.765093944583917 1101 10 4.4092553924930264 1101 11 7.116100194806549 1101 12 4.749942319246664 1101 13 8.981219820234525 1101 14 6.487147693753226 1101 15 5.655994799394481 1101 16 5.17590499585971 1101 17 1.7172467635946127 1101 18 3.766658886004368 1101 19 4.223553420147283 1101 20 2.86396508016324 1101 21 0.36356947835842135 1101 22 3.4258440034718483 1101 23 4.702184738268279 1101 24 5.854206622652896 1101 25 6.2831183191723525 1102 1 4.808214724379816 1102 2 9.530149276944906 1102 3 6.086429708032302 1102 4 7.337561157401485 1102 5 6.889362532419842 1102 6 5.968273462243225 1102 7 5.858770148205597 1102 8 6.19910001923116 1102 9 5.42442826406273 1102 10 4.7504134275137595 1102 11 7.191711624756998 1102 12 4.860336385997865 1102 13 8.945147580948085 1102 14 6.5341535427523 1102 15 4.463514356210791 1102 16 5.148919767572057 1102 17 5.878284392622195 1102 18 5.7849805066156 1102 19 4.71090734330766 1102 20 5.243100130740004 1102 21 3.7093854886062907 1102 22 4.954557320637566 1102 23 5.370931970794274 1102 24 6.648594750983968 1102 25 6.185057355558104 1103 1 3.864753557725045 1103 2 8.017295659181892 1103 3 5.468448267761023 1103 4 6.971593670447579 1103 5 6.219801179562246 1103 6 5.827463252878587 1103 7 6.035622823059995 1103 8 5.487592208876897 1103 9 4.720868135256225 1103 10 3.97635141613398 1103 11 5.033900946100687 1103 12 3.4573640612307273 1103 13 7.25182428223853 1103 14 5.748378703273843 1103 15 4.719713007159431 1103 16 4.550417175654687 1103 17 2.3829708214423277 1103 18 1.4644402372811065 1103 19 3.2839330202663035 1103 20 3.489297678745082 1103 21 5.524940885568654 1103 22 1.3321878950981514 1103 23 3.7467102488614863 1103 24 4.819771591764333 1103 25 1.7054682993533987 1104 1 4.716936945380848 1104 2 8.864757531694723 1104 3 5.954083339726555 1104 4 6.352142155210672 1104 5 6.337090754344096 1104 6 5.966782390356709 1104 7 6.118150712248415 1104 8 5.97031390561661 1104 9 4.934166025916202 1104 10 3.907422930807307 1104 11 6.92750247902762 1104 12 3.9027018781248586 1104 13 7.844399480507725 1104 14 5.809554886548781 1104 15 5.187427925331313 1104 16 4.891836260219279 1104 17 5.1502749552728595 1104 18 3.013975443565606 1104 19 3.975581762082708 1104 20 3.138574892424952 1104 21 2.0577394478234616 1104 22 3.955678372227922 1104 23 4.3261008978819575 1104 24 5.250229434708203 1104 25 4.514159268672941 1105 1 2.863016039632466 1105 2 8.369133087192617 1105 3 5.631179503248859 1105 4 5.266433567562959 1105 5 1.7800640461832948 1105 6 4.335389299567684 1105 7 5.297041512363801 1105 8 2.2307979665472573 1105 9 3.3265923796776136 1105 10 3.189993613542523 1105 11 6.1123294434996644 1105 12 3.4836212916920566 1105 13 4.359616625176038 1105 14 2.9475773216684082 1105 15 3.54525378732923 1105 16 3.88992537289295 1105 17 4.491550986406686 1105 18 2.077131897719827 1105 19 1.9677036762871225 1105 20 0.9955198435055266 1105 21 2.0745736732603426 1105 22 3.491955469205454 1105 23 0.9578307851060295 1105 24 3.6547814776431338 1105 25 4.323602186001629 1106 1 6.495232996150531 1106 2 10.539698127040241 1106 3 7.5744511719641565 1106 4 9.017153739797418 1106 5 8.4713035383344 1106 6 7.5197911972109495 1106 7 7.518905797143606 1106 8 6.83114229617647 1106 9 6.274469867573597 1106 10 5.675175550613073 1106 11 7.837126897390487 1106 12 5.157641317728192 1106 13 3.960294738655584 1106 14 5.3803571840062006 1106 15 5.553220327363643 1106 16 5.594367483779823 1106 17 6.063658993090325 1106 18 6.004291904544736 1106 19 5.996581202486173 1106 20 5.558945490541291 1106 21 5.588069036848452 1106 22 6.152778348372216 1106 23 6.59011390146328 1106 24 7.143134610013053 1106 25 6.18397607365824 1107 1 3.4986860679926544 1107 2 -12.596507571361427 1107 3 -4.942738715622328 1107 4 1.5175923573532941 1107 5 3.8222691340554498 1107 6 4.385937371044644 1107 7 4.135991690712935 1107 8 4.7632011718845035 1107 9 3.20529326299296 1107 10 2.8011278897923546 1107 11 5.354566233397019 1107 12 2.858414950883173 1107 13 4.157704003630574 1107 14 3.385084452100031 1107 15 3.3042325782705255 1107 16 3.0985615497498635 1107 17 3.5635761703339632 1107 18 -1.1378951651077287 1107 19 1.3688160741010975 1107 20 1.6675119119415136 1107 21 -1.5523939472603594 1107 22 1.259911789064752 1107 23 -1.2079465758114303 1107 24 1.8475671467123576 1107 25 2.53695832998536 1108 1 4.941555610298021 1108 2 9.89456385721 1108 3 0.30042511983699427 1108 4 5.686483497947538 1108 5 6.716908697594874 1108 6 6.878853217539476 1108 7 6.870375166898242 1108 8 6.497311472726118 1108 9 5.253687921930638 1108 10 4.63147437687368 1108 11 6.485334882942112 1108 12 2.5994960665235247 1108 13 3.0272182164981025 1108 14 4.60625476627105 1108 15 4.512341817409386 1108 16 4.611534472454803 1108 17 0.21577164763522205 1108 18 3.1300297872318907 1108 19 4.297304193879768 1108 20 4.6358652264690186 1108 21 4.512454198247166 1108 22 1.3186745178733226 1108 23 4.104342246085995 1108 24 4.91645189384111 1108 25 5.31149470686659 1109 1 2.8714290915633676 1109 2 -12.596507571361427 1109 3 -5.056807449520843 1109 4 1.5247039409591991 1109 5 0.6219588579983544 1109 6 1.745630578294353 1109 7 3.04659201749946 1109 8 3.09432371280581 1109 9 2.6593136131558985 1109 10 2.085760365688575 1109 11 0.33458453585879155 1109 12 0.8516859269842634 1109 13 1.8243102661329793 1109 14 2.80348094302078 1109 15 2.816928023494519 1109 16 2.7178164845184014 1109 17 3.2709441696171666 1109 18 3.300012374238033 1109 19 1.6161217966909476 1109 20 1.005734962371946 1109 21 0.46881043123616095 1109 22 2.437839302054165 1109 23 2.796133725484541 1109 24 3.4714253821657763 1109 25 3.8476889284194833 1110 1 2.862169747933436 1110 2 8.397109449915169 1110 3 5.851692558072518 1110 4 6.850454544631351 1110 5 7.740955926961252 1110 6 3.524879443086977 1110 7 5.840581113433977 1110 8 6.7806905936052395 1110 9 5.002984547291092 1110 10 4.752156708726222 1110 11 3.0426365351905744 1110 12 5.390812994276622 1110 13 9.487713306514468 1110 14 7.384558288989467 1110 15 6.400573443938885 1110 16 6.0531134608423045 1110 17 6.428623334954995 1110 18 6.5732883017670005 1110 19 5.508065241342944 1110 20 5.794996432142778 1110 21 3.298905955709561 1110 22 5.447697002480911 1110 23 6.063506574665215 1110 24 6.747784214003218 1110 25 7.111579462536451 1111 1 5.453552076455278 1111 2 9.09813276714351 1111 3 7.593227433884017 1111 4 8.059905474419583 1111 5 8.533834719386903 1111 6 7.747009303343656 1111 7 7.516809744309548 1111 8 7.995218097131332 1111 9 6.45776152815516 1111 10 5.535260470892496 1111 11 7.324947051450614 1111 12 6.64191283522732 1111 13 8.336829532544538 1111 14 6.519075989476619 1111 15 6.022145131161924 1111 16 5.86724984312081 1111 17 7.072038793747222 1111 18 6.900186640251931 1111 19 5.647377428120844 1111 20 5.545509091067043 1111 21 7.94266952241524 1111 22 7.396724200912427 1111 23 7.366101379054577 1111 24 7.057722294808265 1111 25 7.650498527446572 1112 1 -0.8628726347242832 1112 2 7.989772485215658 1112 3 6.10575778222411 1112 4 0.06509436727622742 1112 5 4.136041544945542 1112 6 3.3749431424660337 1112 7 5.827813149707513 1112 8 6.240803747777797 1112 9 5.111497191868619 1112 10 4.029894026530954 1112 11 0.4978403230465638 1112 12 4.3703248664364835 1112 13 8.099115996935485 1112 14 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2204 6 4.300294169291586 2204 7 5.241269026257768 2204 8 4.926756401902807 2204 9 3.6143503281939005 2204 10 3.302112572726468 2204 11 4.830923754013817 2204 12 5.037387127536903 2204 13 3.054544341731951 2204 14 4.261440156703223 2204 15 4.532488337555904 2204 16 4.230031854900684 2204 17 -0.5145416728068533 2204 18 2.9911618597373533 2204 19 3.863703995794591 2204 20 3.7382436241800328 2204 21 5.5247392129463435 2204 22 5.060657767099059 2204 23 3.7765830060413457 2204 24 5.350618049573789 2204 25 5.216528782233283 2205 1 5.450356002106769 2205 2 9.486361509418517 2205 3 6.587147624351978 2205 4 7.161564997228201 2205 5 7.389097928976305 2205 6 6.94997377525474 2205 7 7.068053011337927 2205 8 6.6152309835773835 2205 9 5.603847859486116 2205 10 4.953189917600362 2205 11 6.9794596648454466 2205 12 6.151045318107595 2205 13 9.018982197642305 2205 14 6.739659140448406 2205 15 5.788291494473267 2205 16 5.4374780275069945 2205 17 6.27484906047308 2205 18 5.911910163123409 2205 19 4.495370403436226 2205 20 4.847042106691859 2205 21 -0.7778458568509379 2205 22 3.626208035887341 2205 23 4.607558215353496 2205 24 6.357707365389061 2205 25 6.760331907270643 2206 1 4.3343180203018115 2206 2 8.386602914212114 2206 3 5.991335502557721 2206 4 2.518383432987868 2206 5 6.026346796441839 2206 6 6.154874302365979 2206 7 6.21662036258925 2206 8 6.16239361964324 2206 9 5.1301495145252325 2206 10 4.163121916526144 2206 11 3.793030573950613 2206 12 5.239392807713369 2206 13 8.180316657466001 2206 14 0.6194463161005324 2206 15 2.851160634060715 2206 16 3.753579619426059 2206 17 4.874787508621527 2206 18 -0.30391434745116186 2206 19 1.496628883968004 2206 20 3.0095017500439667 2206 21 3.9380178587246206 2206 22 -1.1659618433024477 2206 23 -2.1225530876443166 2206 24 1.357602492011413 2206 25 2.931957679854193 2207 1 0.9272313380790109 2207 2 -12.596507571361427 2207 3 -5.504320879777428 2207 4 -4.324543589000603 2207 5 0.7038973230045507 2207 6 1.2253796297237827 2207 7 2.706053304550064 2207 8 2.769462523429798 2207 9 0.7139587914120327 2207 10 0.35896521936452075 2207 11 -1.0696665629803839 2207 12 -2.2780559866864216 2207 13 0.328915247537239 2207 14 -4.954986317206874 2207 15 -1.5254063992862008 2207 16 -0.3471776925731511 2207 17 0.7922590199120698 2207 18 0.7254047631331808 2207 19 0.036501224918904285 2207 20 -0.11031898405729734 2207 21 -3.6143527229018595 2207 22 -4.522582952726253 2207 23 -1.762704698197794 2207 24 -0.061831314178942165 2207 25 0.5220012617152499 2208 1 4.601799777895131 2208 2 5.999749113130553 2208 3 5.864952504217321 2208 4 7.541139870986264 2208 5 3.2713689432315896 2208 6 5.831220702995196 2208 7 6.266277059123663 2208 8 6.201412518738927 2208 9 4.617551999030006 2208 10 4.237779836120314 2208 11 6.31680121073197 2208 12 5.825398840220416 2208 13 7.844500199221262 2208 14 2.796670967716665 2208 15 4.296080137912412 2208 16 4.702370107408086 2208 17 5.46666046470051 2208 18 0.46722200677705317 2208 19 2.9905888503997176 2208 20 4.160932071388803 2208 21 6.649982111510372 2208 22 5.754345464573678 2208 23 1.4343982195243186 2208 24 2.6829721383025316 2208 25 3.873620081491964 2209 1 3.3737900425324554 2209 2 4.777424052570199 2209 3 5.028421660333535 2209 4 0.7562586302226677 2209 5 5.048308778994954 2209 6 5.420545321731581 2209 7 5.776536135559212 2209 8 4.345220508135153 2209 9 4.117902411145124 2209 10 3.1181959885952892 2209 11 -0.4765851414779121 2209 12 2.9787441332715936 2209 13 1.9661427179226045 2209 14 3.140926113442397 2209 15 3.7604085654761334 2209 16 3.073886934807266 2209 17 0.6755803574439341 2209 18 2.6381697953718977 2209 19 3.292674564250138 2209 20 3.443023346127138 2209 21 5.661696159989127 2209 22 4.489334876033681 2209 23 -0.16303158378783777 2209 24 -3.273668171278956 2209 25 -0.5046174217455395 2210 1 -2.4011820867875002 2210 2 -12.596507571361427 2210 3 -7.1492941825997 2210 4 -2.664947860549038 2210 5 -1.138307101099187 2210 6 -1.5884723916339603 2210 7 -1.425067976914466 2210 8 -18.819309406394762 2210 9 -11.995766609045633 2210 10 -6.793063766142726 2210 11 -8.879780979456358 2210 12 -4.459711211705493 2210 13 -5.0662182383513965 2210 14 -3.968556111257218 2210 15 -5.418236300125255 2210 16 -5.087589591886651 2210 17 -4.63758079702157 2210 18 -4.6560826510544615 2210 19 -9.461530811694368 2210 20 -6.88895361411069 2210 21 -5.1128424254190215 2210 22 -9.357884249446316 2210 23 -7.784535361813353 2210 24 -10.283806905330733 2210 25 -8.272262658955993 2211 1 -0.1435394976397255 2211 2 4.038010195136037 2211 3 0.3066064385183118 2211 4 0.9486195218221383 2211 5 0.8433520511955876 2211 6 -0.17865431300105072 2211 7 -18.672894063870988 2211 8 -12.139831523871194 2211 9 -8.453060279106905 2211 10 -6.91251653069591 2211 11 -8.543005748507655 2211 12 -4.762907709903093 2211 13 -6.288796035747115 2211 14 -4.4744998217804905 2211 15 -4.912157541100125 2211 16 -10.935629023947271 2211 17 -7.278343374398581 2211 18 -5.483890556383884 2211 19 -7.666779311447392 2211 20 -6.152341807613649 2211 21 -3.9565834265645705 2211 22 -5.201898500478887 2211 23 -7.137224245797757 2211 24 -19.565690963301748 2211 25 -19.3650162821444 2212 1 -1.0657856479894896 2212 2 -12.596507571361427 2212 3 -7.161004678600753 2212 4 -17.77436815040411 2212 5 -17.84627811559194 2212 6 -18.727602826416568 2212 7 -9.00521806084298 2212 8 -8.663366938567359 2212 9 -4.8715200457262355 2212 10 -5.062402431405097 2212 11 -5.335973587164615 2212 12 -2.729484330445139 2212 13 -5.236658945325692 2212 14 -3.5136630241141873 2212 15 -3.5234036634534327 2212 16 -3.688903923365878 2212 17 -3.6179623700503463 2212 18 -2.9250296499244435 2212 19 -3.425363656816092 2212 20 -3.572925462403049 2212 21 -1.5357126708413524 2212 22 -2.5311192821948936 2212 23 -2.7263372466392966 2212 24 -1.8064176854819145 2212 25 -2.143214616739403 2213 1 -16.69031252157011 2213 2 -12.596507571361427 2213 3 -7.97079043683293 2213 4 -5.5367599678706725 2213 5 -17.84627811559194 2213 6 -9.829884494394538 2213 7 -18.672894063870988 2213 8 -9.777609957142205 2213 9 -7.865372305803865 2213 10 -6.76557713573645 2213 11 -5.943493222469391 2213 12 -4.971724461571512 2213 13 -17.131435629655744 2213 14 -11.195074657648854 2213 15 -6.57464515313384 2213 16 -5.7694582562955965 2213 17 -4.631021999315781 2213 18 -4.595212515705139 2213 19 -5.283015663289028 2213 20 -5.364282295167488 2213 21 -3.45002072847835 2213 22 -4.341067638082446 2213 23 -4.125226817816273 2213 24 -3.441379397605445 2213 25 -4.2249690752207165 2214 1 -16.69031252157011 2214 2 -12.596507571361427 2214 3 -10.877120269556222 2214 4 -17.77436815040411 2214 5 -17.84627811559194 2214 6 -18.727602826416568 2214 7 -14.633763629088378 2214 8 -13.171483319438241 2214 9 -12.102578203248532 2214 10 -12.025092751649971 2214 11 -12.81456358815201 2214 12 -10.655218458082304 2214 13 -17.131435629655744 2214 14 -14.700005386619537 2214 15 -21.300844995777428 2214 16 -16.054256258553316 2214 17 -21.02598659412302 2214 18 -15.558546419170403 2214 19 -12.858632260969731 2214 20 -11.607939121357068 2214 21 -9.280958367448008 2214 22 -10.269188597272402 2214 23 -10.164825216885816 2214 24 -9.205690963301748 2214 25 -8.922407586492225 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 195758.0 3 14.0 4 984.0 5 3591.0 6 4290.0 7 4096.0 8 3627.0 9 3469.0 10 3274.0 11 3065.0 12 2819.0 13 3138.0 14 4278.0 15 8152.0 16 11642.0 17 12447.0 18 13110.0 19 15378.0 20 18503.0 21 21006.0 22 21858.0 23 23829.0 24 24434.0 25 24372.0 26 20620.0 27 17355.0 28 18659.0 29 21181.0 30 20406.0 31 23530.0 32 29353.0 33 34927.0 34 40967.0 35 44788.0 36 42510.0 37 41474.0 38 24245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.66811706264855 19.03352269322841 13.277828343495615 26.020531900627425 2 12.77108715068797 20.283205196311364 43.06123918327 23.88446846973067 3 18.84757376022965 25.655817462011633 29.11959182230231 26.377016955456405 4 11.240553524055352 17.431992543199254 38.645730264573025 32.68172366817237 5 11.57454473600559 37.867454387688184 36.46665311160304 14.091347764703189 6 29.691764572731984 37.99712992668109 19.685975092174623 12.625130408412305 7 25.989366209696158 31.069256318084275 24.685291745827808 18.256085726391756 8 24.212639608613088 38.89715901166221 20.840709388779814 16.049491990944887 9 26.428568770859716 15.260970458116855 20.56657807037018 37.743882700653245 10 14.396834369700395 29.745870234281767 35.352427611330945 20.50486778468689 11 32.988734729802474 21.677098927697163 26.010842216799805 19.32332412570056 12 24.434561976680236 24.406997242213947 32.610262219381156 18.54817856172466 13 28.82638578609842 21.789035807553752 25.020459984693723 24.36411842165411 14 19.506677939148727 23.562255301589218 28.218822025624767 28.712244733637288 15 21.605269067450596 33.710624897925854 23.334405111873266 21.349700922750287 16 20.204512859250034 28.120007757912308 30.529554073159154 21.145925309678507 17 20.167575248148612 29.07286423828389 30.418134555092692 20.341425958474808 18 20.170076364664844 27.187329122277742 33.19304861569404 19.449545897363375 19 22.637835338812494 26.489159357074122 27.614430404554852 23.258574899558536 20 23.12183182586224 28.34273432064407 28.686512275121757 19.848921578371932 21 21.183179473395843 26.14645467420632 26.878624667900304 25.791741184497535 22 22.066151894656304 29.697126807071232 28.13003362461241 20.106687673660048 23 20.759956491703285 29.001603655292246 29.398114557791743 20.840325295212722 24 22.488602966699837 27.505067238753046 29.62820284200969 20.378126952537432 25 21.614866926471574 27.96640231393396 29.524983689841562 20.893747069752905 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 790.0 1 790.0 2 1756.0 3 2722.0 4 2722.0 5 2722.0 6 3802.0 7 4882.0 8 4882.0 9 4882.0 10 5751.0 11 6620.0 12 6620.0 13 6620.0 14 8277.5 15 9935.0 16 9935.0 17 9935.0 18 14681.5 19 19428.0 20 19428.0 21 19428.0 22 27110.5 23 34793.0 24 34793.0 25 34793.0 26 46148.5 27 57504.0 28 57504.0 29 57504.0 30 69653.0 31 81802.0 32 81802.0 33 81802.0 34 88962.5 35 96123.0 36 96123.0 37 96123.0 38 98305.5 39 100488.0 40 100488.0 41 100488.0 42 99816.5 43 99145.0 44 99145.0 45 99145.0 46 96588.5 47 94032.0 48 94032.0 49 94032.0 50 84818.0 51 75604.0 52 75604.0 53 75604.0 54 63716.0 55 51828.0 56 51828.0 57 51828.0 58 42800.0 59 33772.0 60 33772.0 61 33772.0 62 26795.5 63 19819.0 64 19819.0 65 19819.0 66 15041.5 67 10264.0 68 10264.0 69 10264.0 70 7486.0 71 4708.0 72 4708.0 73 4708.0 74 3250.5 75 1793.0 76 1793.0 77 1793.0 78 1264.0 79 735.0 80 735.0 81 735.0 82 494.0 83 253.0 84 253.0 85 253.0 86 168.0 87 83.0 88 83.0 89 83.0 90 51.5 91 20.0 92 20.0 93 20.0 94 11.5 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.156306952000188 2 36.33108632978546 3 5.396773086505713 4 13.603312399569347 5 13.474959394114347 6 14.615393192582783 7 9.356265076212694 8 7.618543788073826 9 0.10778679029522431 10 1.375086879869764 11 5.4081712298472775 12 5.613090024270612 13 15.981683679221556 14 1.5230149575852785 15 2.8997124446663505 16 2.9031814448137827 17 2.867252514715375 18 1.4401306326341232 19 1.3830160230638953 20 0.2811129048044413 21 4.123773925260392 22 1.5477935300669394 23 0.10729121884559109 24 8.985701524749459 25 0.11187525475469833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 807149.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.97666887536016 #Duplication Level Percentage of deduplicated Percentage of total 1 96.93663789157166 88.18972407352207 2 2.1202268799482686 3.857823575953833 3 0.40694639353872125 1.1106788188498273 4 0.17176549359634047 0.6250660974050826 5 0.0845253686370696 0.3844918237031217 6 0.0587603347996823 0.32074917132455993 7 0.0418280862071487 0.26637659639904504 8 0.026724772973999767 0.19450646572998007 9 0.020335145504628425 0.16650214191961643 >10 0.11231393699515801 2.0076965803331466 >50 0.01046355730447574 0.6492275280484237 >100 0.008482512494433377 1.4499158258178013 >500 5.655008162194087E-4 0.32836290607915597 >1k 4.2412561216455654E-4 0.4488783949143182 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1310 0.16229964975487798 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1148 0.14222900604473276 No Hit CTGTAGGACGTGGAATATGGCAAGA 1032 0.12785743400536953 No Hit CTTTAGGACGTGAAATATGGCGAGG 843 0.10444168301020011 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.238928624083038E-4 0.0 0.0 0.0 0.0 9 1.238928624083038E-4 0.0 0.0 0.0 0.0 10 1.238928624083038E-4 0.0 0.0 0.0 0.0 11 1.238928624083038E-4 0.0 0.0 0.0 0.0 12 1.238928624083038E-4 0.0 0.0 0.0 1.238928624083038E-4 13 1.238928624083038E-4 0.0 0.0 0.0 1.238928624083038E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAGAC 45 2.2681133E-4 17.055775 3 TGTAGGA 790 0.0 15.991644 2 CTGTAGG 825 0.0 15.25957 1 CTAGGAC 115 3.8198777E-11 15.254854 3 AGGACGG 70 2.7762617E-6 14.8880205 5 GTATTAT 55 0.004466464 14.610229 1 GTTCCTA 65 8.114182E-4 14.422917 1 GTCCTAC 920 0.0 14.411719 1 TGTAGAA 240 0.0 14.304119 2 GACCTAC 85 2.6812095E-5 14.180514 1 TACTGGA 70 0.0011760221 13.731955 2 CATAATA 60 0.006737527 13.731955 2 GTCCTAA 570 0.0 13.627668 1 TCCTACA 975 0.0 13.520694 2 GTAGGAC 875 0.0 13.4079275 3 TAGGACC 265 0.0 13.3913555 4 CTGTAGA 195 3.6379788E-12 13.049306 1 ACCTACA 95 6.029367E-5 13.00922 2 ACCAATC 45 0.009648276 13.007033 8 TCCTAAA 645 0.0 12.98681 2 >>END_MODULE