FastQCFastQC Report
Thu 26 May 2016
SRR1926675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807149
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA31940.3957138025321223No Hit
GTCCTACAGTGGACATTTCTAAATT29480.3652361583796796No Hit
CTTTAGGACGTGAAATATGGCGAGG28210.349501764853825No Hit
GTCCTAAAGTGTGTATTTCTCATTT26350.3264576924458805No Hit
GTCCTACAGTGTGCATTTCTCATTT16890.2092550446076251No Hit
CTGAAGGACCTGGAATATGGCGAGA15770.1953790440178951No Hit
CCCATGTACTCTGCGTTGATACCAC15010.18596318647486398No Hit
CTGTAGGACCTGGAATATGGCGAGA14780.18311365063947302No Hit
GTACATGGGAAGCAGTGGTATCAAC13910.17233497160995057No Hit
GTATCAACGCAGAGTACTTTTTTTT13560.16799872142565994No Hit
GAATATGGCAAGAAAACTGAAAATC9070.11237082620433154No Hit
GTCCTTCAGTGTGCATTTCTCATTT8540.10580450449669143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT755.615766E-815.2471474
TAGGACC6150.013.790614
TATGTCG852.7745227E-713.37918416
CCGGTTT802.0327825E-613.04127112
CCAACGA957.613562E-812.96761719
TCCTATA1501.8189894E-1212.7154622
CCTAGAC751.4237994E-512.7114993
GCTATAC603.9681353E-412.7114993
GGACCTG11350.012.6779696
CGGTTTC905.501588E-712.64608213
GTCGAGG604.1751555E-412.63511319
AGGACCT11700.012.6245075
TAGGACA4850.012.5749674
TGTAGGA16450.012.1743772
TATACTC550.002995919412.1284115
TAATCTG550.002995919412.1284115
GTAGGAC16550.012.0394413
CTACACC959.995838E-712.0372214
TGTCGAG951.0686344E-611.9708518
TCCAACG1353.8562575E-1011.93390118
Produced by FastQC (version 0.11.5)