FastQCFastQC Report
Thu 26 May 2016
SRR1926674_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926674_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718912
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12170.1692835840826137No Hit
GTCCTAAAGTGTGTATTTCTCATTT10420.14494124454731594No Hit
CTGTAGGACGTGGAATATGGCAAGA9290.12922304816166652No Hit
CTTTAGGACGTGAAATATGGCGAGG8180.11378304994213478No Hit
GTCCTACAGTGTGCATTTCTCATTT7970.11086196919789905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG459.978043E-418.2843272
GCTCTAG654.4219712E-516.4499511
ATAAGGG400.002040398516.4335923
GTGCTAG500.002399286516.0387021
TATAGGA1301.3460522E-1015.8229742
TGTAGGA6950.015.7850292
GTCCTAA4550.015.5687031
CTAAGAC505.090034E-415.3380183
GTCGTCA708.331839E-515.2749531
GTATAGA708.331839E-515.2749531
TAGGACC2800.014.9741394
TCGTCAA852.1436499E-514.5199062
GGATAAT658.2370103E-414.3937061
ATAGGAC1156.2755134E-1014.2900793
CTGTAGG7500.014.2566231
GATATAC2350.014.2187071
TCCTAAA5500.013.9625762
TGTAGAA2850.013.9538282
GTCCTAC7800.013.3655851
GTGTTAG600.00805581313.3655841
Produced by FastQC (version 0.11.5)