FastQCFastQC Report
Thu 26 May 2016
SRR1926674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718912
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA26220.36471779578029023No Hit
GTCCTACAGTGGACATTTCTAAATT25650.3567891480459361No Hit
CTTTAGGACGTGAAATATGGCGAGG23520.3271610433544022No Hit
GTCCTAAAGTGTGTATTTCTCATTT23000.3199278910353423No Hit
GTCCTACAGTGTGCATTTCTCATTT17480.24314519718686015No Hit
CTGTAGGACCTGGAATATGGCGAGA15400.21421258791062048No Hit
CTGAAGGACCTGGAATATGGCGAGA13320.18527997863438084No Hit
GTATCAACGCAGAGTACTTTTTTTT9120.12685836374966616No Hit
CCCATGTACTCTGCGTTGATACCAC8750.12171169767648891No Hit
GATATACACTGTTCTACAAATCCCG8320.1157304371049586No Hit
GTCCTTCAGTGTGCATTTCTCATTT7790.10835818570283985No Hit
GTACATGGGAAGCAGTGGTATCAAC7730.10752359120448678No Hit
GAATATGGCAAGAAAACTGAAAATC7720.10738449212142794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACC500.001453208213.3538754
TAGGACC5350.013.1934164
AACTGTA655.4521926E-513.1518677
TAATACT1102.575689E-913.0070234
GGCGAGG6150.012.93936519
GATATAC3550.012.8916871
TTAATAC905.128968E-712.7188693
TATGTCG751.5190231E-512.63128616
GTCCTAA7100.012.4888221
TCCAACG1457.2759576E-1212.41350618
ATACAAT1001.3644058E-712.4000276
ACGTTTT854.059968E-612.25977819
GTAGGTC550.002972655212.1407383
CCCTTAA550.002983592512.13478851
GGACCTG8750.012.1002466
GACTGTC550.003069131412.0890897
AGGACCT9350.011.9358575
GTCGAGG802.9444165E-511.84183119
CAAGAGT657.7468436E-411.7396724
GTATTAG657.774207E-411.734741
Produced by FastQC (version 0.11.5)