FastQCFastQC Report
Thu 26 May 2016
SRR1926673_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926673_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657302
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12470.18971492555933195No Hit
GTCCTAAAGTGTGTATTTCTCATTT10130.15411485131644206No Hit
CTTTAGGACGTGAAATATGGCGAGG8650.13159856504316128No Hit
CTGTAGGACGTGGAATATGGCAAGA8170.12429598571128644No Hit
GTCCTACAGTGTGCATTTCTCATTT7540.11471135033820071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA7050.016.9515512
CTGTAGG7050.015.9620231
TAGGACC3450.015.4661144
TTTAGGA4650.014.8947482
GTCGAGG350.00424563914.72316919
CCCCTAC550.00482023514.4426441
CTTTAGG5050.013.8944921
GTAGGAC8150.013.7158123
TGTCCAA751.4611487E-613.42823710
CTCTAGG802.8083485E-413.2390921
GTGTAAA905.1121777E-513.2390911
GTATCAA3500.013.2390911
GCCTTAT700.001548604813.239091
GCTCTAA600.00857705313.239091
TAGGACG8050.012.858974
AGGACCT5900.012.8097835
TTAGGAC5100.012.7680033
GATATAC1359.458563E-812.7487541
AGGACGT7800.012.7422915
GGACCTG5850.012.74099356
Produced by FastQC (version 0.11.5)