##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926673_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 657302 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.5422956266678 26.0 2.0 30.0 2.0 31.0 2 15.454331494503288 15.0 2.0 30.0 2.0 31.0 3 18.674239542858533 16.0 7.0 30.0 2.0 31.0 4 20.762191504057494 27.0 2.0 33.0 2.0 35.0 5 20.858349738780653 27.0 2.0 35.0 2.0 35.0 6 21.842576167423804 29.0 2.0 35.0 2.0 36.0 7 21.692104998919827 29.0 2.0 35.0 2.0 37.0 8 21.752517107813457 28.0 2.0 35.0 2.0 37.0 9 24.301283124043437 30.0 7.0 37.0 2.0 39.0 10 24.920383628834234 31.0 2.0 37.0 2.0 39.0 11 22.258152264864552 28.0 2.0 35.0 2.0 39.0 12 23.32146562767191 30.0 2.0 35.0 2.0 39.0 13 20.197654959212052 25.0 2.0 35.0 2.0 39.0 14 23.217685325771107 29.0 2.0 37.0 2.0 40.0 15 24.247142105151056 30.0 2.0 37.0 2.0 40.0 16 24.58052919358225 31.0 2.0 38.0 2.0 40.0 17 23.91789770912001 30.0 2.0 37.0 2.0 40.0 18 23.92359372099887 30.0 2.0 37.0 2.0 40.0 19 24.257120775533924 30.0 2.0 37.0 2.0 40.0 20 24.505362831696846 31.0 2.0 38.0 2.0 40.0 21 22.452286772290364 30.0 2.0 36.0 2.0 40.0 22 23.32543184107153 30.0 2.0 37.0 2.0 40.0 23 23.267441754322974 30.0 2.0 37.0 2.0 40.0 24 22.44629257175545 30.0 2.0 37.0 2.0 40.0 25 22.237349041992875 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.458629470949738 1101 2 8.813026643801557 1101 3 5.737915599884751 1101 4 7.190647758842292 1101 5 6.801698770800115 1101 6 6.58103460521853 1101 7 5.983310605140918 1101 8 4.5844320120873086 1101 9 4.67147291722107 1101 10 4.436859668375295 1101 11 6.860895888691516 1101 12 4.861699103344808 1101 13 9.116378647762563 1101 14 6.58322983720651 1101 15 5.790496938184869 1101 16 5.609859793656103 1101 17 1.2946642333915968 1101 18 3.7114520602011787 1101 19 4.245106580387965 1101 20 2.3203075153021153 1101 21 -0.16213376561601933 1101 22 3.338809722674956 1101 23 4.616190500724741 1101 24 5.764812842760421 1101 25 6.267428724751518 1102 1 5.312764788404831 1102 2 9.756000691407024 1102 3 6.449840657172462 1102 4 7.98101859716591 1102 5 7.555439837645775 1102 6 6.678814898245001 1102 7 6.363720766160704 1102 8 6.485458438284503 1102 9 5.5812427573419185 1102 10 5.252532884925049 1102 11 7.669638278967447 1102 12 4.674756899957703 1102 13 9.238718443237747 1102 14 6.886855451212352 1102 15 4.608341019088137 1102 16 5.210414903193723 1102 17 6.323857935325854 1102 18 6.1404423487089765 1102 19 4.905266503409656 1102 20 5.3985156638304765 1102 21 3.9340957011073883 1102 22 5.284471576734962 1102 23 5.7345629300893926 1102 24 7.161453669633804 1102 25 6.758933910989597 1103 1 3.7683642619810698 1103 2 7.896325062649829 1103 3 5.208411521824235 1103 4 6.797309442591512 1103 5 5.8745024063833995 1103 6 5.776009255112616 1103 7 5.888011578999578 1103 8 5.253410577269854 1103 9 4.5293780189207595 1103 10 3.7719438353984067 1103 11 4.437308246521436 1103 12 3.302162485470827 1103 13 7.251756096460166 1103 14 5.802118111340935 1103 15 4.898825028262781 1103 16 4.680704325171842 1103 17 2.2310057598285553 1103 18 1.5335078932072648 1103 19 3.3059373456210217 1103 20 3.5967222088745885 1103 21 5.827084592736526 1103 22 1.9887870221770036 1103 23 3.92651652659017 1103 24 4.927753513439697 1103 25 1.1909309663344523 1104 1 4.824859521163507 1104 2 9.068787115324284 1104 3 6.067280216383725 1104 4 6.552788129879879 1104 5 6.578025000475922 1104 6 6.082829599969628 1104 7 6.103968381075461 1104 8 6.0869470207778775 1104 9 4.903661572393432 1104 10 4.010526454564278 1104 11 6.737663342909428 1104 12 4.2532592439491275 1104 13 8.078807600387083 1104 14 5.9912271760349896 1104 15 5.212663431328178 1104 16 4.618828287286142 1104 17 5.016068519778582 1104 18 2.8726027000775645 1104 19 3.5912900024990186 1104 20 2.794298451766643 1104 21 1.7974669722542664 1104 22 3.9800354168043945 1104 23 4.090948252034806 1104 24 5.135041850394007 1104 25 4.3753148934277455 1105 1 3.125515477720814 1105 2 8.560032543739414 1105 3 5.649669119970515 1105 4 4.947044620437012 1105 5 1.7454376752285832 1105 6 4.522777203745768 1105 7 5.637814061720906 1105 8 3.1054176217499467 1105 9 3.643368202293839 1105 10 3.444269393298409 1105 11 6.195252729447731 1105 12 3.630293379644449 1105 13 4.777848365194146 1105 14 3.792988343033972 1105 15 4.188359556172863 1105 16 4.389123758110092 1105 17 4.744096412809412 1105 18 2.463762307091546 1105 19 2.17473572100052 1105 20 1.4227391048696596 1105 21 2.2130533432135095 1105 22 3.6592031858136203 1105 23 1.3471262099156434 1105 24 4.158303967020775 1105 25 4.7737592102513915 1106 1 6.297281043604784 1106 2 10.509046609165448 1106 3 7.393522993078271 1106 4 8.872008317328284 1106 5 8.312360286455807 1106 6 7.4260479409705304 1106 7 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2204 4 4.690585890119092 2204 5 0.31066254162685425 2204 6 3.0914012133736897 2204 7 4.332048770949097 2204 8 3.9996272582672745 2204 9 2.480371941709574 2204 10 2.2128819411156506 2204 11 3.85192780144223 2204 12 3.78737606228124 2204 13 1.9455375208885144 2204 14 3.0214143498824058 2204 15 3.0171652355000553 2204 16 3.035408698723586 2204 17 -1.5832414383876312 2204 18 1.3843276609033168 2204 19 2.3801070120302192 2204 20 2.5988419459659795 2204 21 4.165610759721382 2204 22 3.5142420843336133 2204 23 2.506426752918923 2204 24 3.9631179275061825 2204 25 4.090645322835027 2205 1 5.77435157190104 2205 2 9.865065342699339 2205 3 6.981104216587799 2205 4 7.458238779404454 2205 5 7.476288470856709 2205 6 7.2065230575321095 2205 7 7.244676385182419 2205 8 6.95438916302918 2205 9 6.1278288608435645 2205 10 5.22692623263541 2205 11 7.365881470926674 2205 12 6.878690506584089 2205 13 9.700648462083311 2205 14 7.288784045307409 2205 15 6.1087522538934955 2205 16 5.89967034153117 2205 17 6.422632002276174 2205 18 6.457429908046596 2205 19 4.6576837221671425 2205 20 5.332731047250263 2205 21 -0.48375771797002187 2205 22 3.9512548138976378 2205 23 5.044802352935914 2205 24 6.791128632234106 2205 25 7.055864868875268 2206 1 3.914955018502038 2206 2 8.194323565776411 2206 3 5.6601273558312535 2206 4 2.3028163084985067 2206 5 5.458263757911556 2206 6 6.105971290517672 2206 7 6.240489561534151 2206 8 6.2066040024533216 2206 9 4.797312647830413 2206 10 4.047475668239922 2206 11 3.280687866761223 2206 12 4.683031786139566 2206 13 7.681409473138078 2206 14 0.43767371133293764 2206 15 2.5056893641109 2206 16 3.3099596306454266 2206 17 4.460716554742611 2206 18 -0.8409468477964985 2206 19 1.050676863936296 2206 20 2.797648185056019 2206 21 3.4754086086993645 2206 22 -1.8213792907438133 2206 23 -2.083789475472315 2206 24 1.7207929092056027 2206 25 3.186151826579607 2207 1 1.7112686215291113 2207 2 -12.64537743090133 2207 3 -5.326278734787209 2207 4 -3.514808475668623 2207 5 1.3973561486274662 2207 6 2.133868579713777 2207 7 3.1813670254480364 2207 8 3.228872541286144 2207 9 1.2357910189546821 2207 10 1.0026935525933958 2207 11 -0.6747009743231018 2207 12 -1.586665278533367 2207 13 0.9333213392447668 2207 14 -4.5731563975665 2207 15 -0.8414169082915564 2207 16 0.09366232549336218 2207 17 1.3723418973683934 2207 18 1.147164536212209 2207 19 0.8807814894555754 2207 20 0.7791207493499961 2207 21 -2.9669633019700434 2207 22 -3.2916029021477655 2207 23 -0.6555699328385565 2207 24 0.8977459473916376 2207 25 1.5192524688070606 2208 1 5.2641673651073155 2208 2 6.18402955528876 2208 3 6.367490572404229 2208 4 8.149271126044134 2208 5 4.070827214140589 2208 6 6.497089712458077 2208 7 6.891955970552559 2208 8 6.658888021874098 2208 9 5.209062272018404 2208 10 5.104379139204568 2208 11 7.049009545649088 2208 12 6.741811618326345 2208 13 8.872104043902738 2208 14 3.5137982461221995 2208 15 4.913094547482871 2208 16 5.233266992242701 2208 17 6.255988070486232 2208 18 1.799039415161257 2208 19 3.8234684792127496 2208 20 5.140187000971558 2208 21 7.474398571752388 2208 22 6.623350704857096 2208 23 2.7844988728710085 2208 24 4.06822470303662 2208 25 5.527772365751769 2209 1 2.546223537851951 2209 2 4.1080009474770485 2209 3 4.275859262987581 2209 4 0.29096953647731283 2209 5 4.276134369386817 2209 6 4.493786119466222 2209 7 4.936463567101182 2209 8 3.9516542852943033 2209 9 3.3838906086816287 2209 10 2.771419504102365 2209 11 -1.0873701829828732 2209 12 2.2940440650297518 2209 13 1.5447759542371173 2209 14 2.844355829497612 2209 15 3.4698937054954726 2209 16 2.8171597294152946 2209 17 0.7264327485111295 2209 18 2.687786204192367 2209 19 3.349753141210247 2209 20 3.366770255631579 2209 21 5.289906453720466 2209 22 3.966369317498984 2209 23 -0.10472475876224863 2209 24 -2.8525858058882285 2209 25 -0.26788651408664066 2210 1 -2.9275452891145495 2210 2 -12.64537743090133 2210 3 -7.422683029304711 2210 4 -2.7252690858659605 2210 5 -1.6495558557220136 2210 6 -2.244527048352758 2210 7 -2.097950207138176 2210 8 -18.950372741732725 2210 9 -12.67198721681829 2210 10 -7.720765995350575 2210 11 -9.701593417845645 2210 12 -5.429463599611633 2210 13 -5.960247965030987 2210 14 -4.996004152577484 2210 15 -6.661254157483786 2210 16 -6.1875179133437435 2210 17 -5.5391911602813835 2210 18 -5.9723914152875786 2210 19 -10.385000507787588 2210 20 -8.105360305161772 2210 21 -6.487092929383568 2210 22 -10.116906545573393 2210 23 -8.460958172408455 2210 24 -10.43286157584423 2210 25 -8.581290033885509 2211 1 0.06228205651233054 2211 2 4.255419734731973 2211 3 0.3546354760917403 2211 4 1.3064924220732728 2211 5 0.9052039837310097 2211 6 -0.23402176481309667 2211 7 -18.761171568033955 2211 8 -12.276324911794726 2211 9 -8.59493168682025 2211 10 -7.000079131379884 2211 11 -8.967033781479511 2211 12 -4.743988126816657 2211 13 -6.056768404559234 2211 14 -4.604028144600488 2211 15 -5.225429080754019 2211 16 -11.075807917150671 2211 17 -7.362773347531768 2211 18 -5.717098529966062 2211 19 -8.37257497743098 2211 20 -6.440481138809808 2211 21 -4.225990477311541 2211 22 -6.009506996148588 2211 23 -7.6656784549296315 2211 24 -19.73518715723958 2211 25 -19.534778405978532 2212 1 -0.8182297559875131 2212 2 -12.64537743090133 2212 3 -7.1942589325833435 2212 4 -17.84585478784701 2212 5 -17.909676441423994 2212 6 -18.80266658323648 2212 7 -8.988829140940823 2212 8 -8.586308771836206 2212 9 -4.910181656198489 2212 10 -5.060422745014108 2212 11 -4.75200653852939 2212 12 -2.5340503452318757 2212 13 -5.415069651010651 2212 14 -3.291012105464631 2212 15 -3.3209273071917202 2212 16 -3.549117825185405 2212 17 -3.8366623609445156 2212 18 -2.8065248990086715 2212 19 -3.26127092377666 2212 20 -3.420107461690634 2212 21 -1.3902297619872463 2212 22 -2.885635939168786 2212 23 -2.909707628508759 2212 24 -1.9355634507485178 2212 25 -2.3372243137866207 2213 1 -16.756141327012912 2213 2 -12.64537743090133 2213 3 -7.605889975122933 2213 4 -4.980375064800521 2213 5 -17.909676441423994 2213 6 -9.443616138132622 2213 7 -18.761171568033955 2213 8 -9.373715966262893 2213 9 -7.247736660278726 2213 10 -6.402877753095485 2213 11 -5.459949178892732 2213 12 -4.389983155811269 2213 13 -17.179886207925044 2213 14 -10.740034291335386 2213 15 -5.892616152285157 2213 16 -5.164456345116211 2213 17 -3.7938166366306874 2213 18 -3.876513258475047 2213 19 -4.751028463836935 2213 20 -4.657434772839029 2213 21 -3.123229790832692 2213 22 -3.747095909597551 2213 23 -3.5395740182591346 2213 24 -2.848935920839974 2213 25 -3.6287447759092935 2214 1 -16.756141327012912 2214 2 -12.64537743090133 2214 3 -11.214775470989704 2214 4 -17.84585478784701 2214 5 -17.909676441423994 2214 6 -18.80266658323648 2214 7 -14.82519950749845 2214 8 -13.552235372698965 2214 9 -12.555983891837515 2214 10 -12.590674618541817 2214 11 -12.945991537165929 2214 12 -11.25723039773336 2214 13 -17.179886207925044 2214 14 -15.034007800430334 2214 15 -21.47520764585588 2214 16 -16.521757694993983 2214 17 -21.169682116353734 2214 18 -15.958998240321996 2214 19 -13.362496725070468 2214 20 -12.278604237021726 2214 21 -9.953515737387988 2214 22 -10.914536945283007 2214 23 -10.866772276303074 2214 24 -9.793394374934573 2214 25 -9.561553726118229 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 149525.0 3 6.0 4 843.0 5 2924.0 6 3584.0 7 3312.0 8 2876.0 9 2825.0 10 2606.0 11 2583.0 12 2351.0 13 2434.0 14 3449.0 15 6549.0 16 9415.0 17 10047.0 18 10145.0 19 12031.0 20 14672.0 21 17041.0 22 18378.0 23 19887.0 24 20151.0 25 20420.0 26 17102.0 27 14918.0 28 15632.0 29 17363.0 30 17481.0 31 19836.0 32 24630.0 33 29791.0 34 34880.0 35 37753.0 36 35565.0 37 34796.0 38 19501.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.272914114887165 19.189035825254138 13.411634038489485 26.12641602136921 2 12.808860257218216 20.15054968139486 43.25700814602152 23.783581915365403 3 19.16565630782975 25.072267245230044 28.906419500989088 26.855656945951118 4 11.184588844163311 17.29753978536342 38.739720238054 32.77815113241927 5 11.78282714139184 37.64034004972097 36.560734340501725 14.01609846838546 6 30.019808702529982 37.925965849236874 19.376305851966695 12.677919596266445 7 26.430768922426317 30.69860739493665 24.227620278788244 18.643003403848788 8 24.32008650986753 39.08116034636716 20.750735251951795 15.848017891813512 9 26.63120043497663 15.1664592811006 20.559194744946215 37.643145538976555 10 14.615811079150612 29.464287085484013 35.351554401360055 20.568347434005325 11 33.1303271181627 21.316769472800782 26.158922434287575 19.393980974748942 12 24.96410573130394 23.89380390109418 32.50540403734914 18.63668633025274 13 28.988520645968908 21.554728907907702 24.91774461322656 24.53900583289683 14 19.444230846126164 23.57074530092244 28.164168591614715 28.820855261336686 15 21.351892384505735 34.132868058855145 23.249028298139162 21.266211258499958 16 20.215024929296835 27.836311648950602 30.932751701709037 21.01591172004353 17 20.072214194962758 29.00120824095065 30.617541914750507 20.309035649336092 18 19.999446773459876 26.98592960499625 33.67090397216656 19.343719649377313 19 22.847251872989936 26.03770860914947 27.447788197782824 23.667251320077774 20 23.39075470633018 28.143694303277 28.768153641713212 19.697397348679605 21 21.176739313647495 25.94182465481526 26.61715014847079 26.264285883066453 22 22.262137550443317 29.853366298095192 28.002713900431853 19.881782251029637 23 20.516995322611844 29.092866944984713 29.521462525302216 20.868675207101223 24 22.697953736654807 27.22220746799809 29.78601211026717 20.29382668507994 25 21.55015901253823 27.97980967234888 29.785454922077765 20.68457639303513 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 440.0 1 440.0 2 942.0 3 1444.0 4 1444.0 5 1444.0 6 2074.0 7 2704.0 8 2704.0 9 2704.0 10 3296.0 11 3888.0 12 3888.0 13 3888.0 14 5350.5 15 6813.0 16 6813.0 17 6813.0 18 10357.0 19 13901.0 20 13901.0 21 13901.0 22 20463.0 23 27025.0 24 27025.0 25 27025.0 26 36274.5 27 45524.0 28 45524.0 29 45524.0 30 56070.0 31 66616.0 32 66616.0 33 66616.0 34 72973.0 35 79330.0 36 79330.0 37 79330.0 38 81722.5 39 84115.0 40 84115.0 41 84115.0 42 83505.0 43 82895.0 44 82895.0 45 82895.0 46 80457.0 47 78019.0 48 78019.0 49 78019.0 50 70456.0 51 62893.0 52 62893.0 53 62893.0 54 52895.5 55 42898.0 56 42898.0 57 42898.0 58 35406.0 59 27914.0 60 27914.0 61 27914.0 62 22133.5 63 16353.0 64 16353.0 65 16353.0 66 12413.0 67 8473.0 68 8473.0 69 8473.0 70 6096.0 71 3719.0 72 3719.0 73 3719.0 74 2608.0 75 1497.0 76 1497.0 77 1497.0 78 1030.5 79 564.0 80 564.0 81 564.0 82 373.5 83 183.0 84 183.0 85 183.0 86 125.0 87 67.0 88 67.0 89 67.0 90 42.5 91 18.0 92 18.0 93 18.0 94 13.5 95 9.0 96 9.0 97 9.0 98 4.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.060514040730135 2 34.81991535093458 3 4.634399408491074 4 12.163967247931696 5 12.489236302338954 6 13.058837490225192 7 8.910363881442624 8 7.145421739170123 9 0.1087780046310524 10 0.9357951139658788 11 4.648700293015995 12 4.422320333727876 13 14.31868456204302 14 1.075304806618571 15 2.3409330870741303 16 2.4171537588505743 17 2.2890543464039363 18 1.0001490943280258 19 0.9438583786448238 20 0.28221426376308 21 3.422627650608092 22 1.1128826627638437 23 0.11242929429698982 24 8.342892612528182 25 0.11410280206054448 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 657302.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.22625038014972 #Duplication Level Percentage of deduplicated Percentage of total 1 97.23488756599632 88.70374198781286 2 1.8448144195085578 3.3659100427799653 3 0.372352156566731 1.0190487319363593 4 0.163297280073216 0.5958799423342656 5 0.09825179976637878 0.4481571642894003 6 0.05455783942944375 0.2986264271994264 7 0.043610986136794934 0.2784926718448345 8 0.024079560553207324 0.17573504160566944 9 0.020560795566835714 0.16881158559557044 >10 0.12290573312138589 2.099956498347648 >50 0.011547469686901839 0.720733890871634 >100 0.008100311301569978 1.306388759618909 >500 6.893881951193393E-4 0.46318706021496736 >1k 3.4469409755966964E-4 0.3553301955484814 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1247 0.18971492555933195 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1013 0.15411485131644206 No Hit CTTTAGGACGTGAAATATGGCGAGG 865 0.13159856504316128 No Hit CTGTAGGACGTGGAATATGGCAAGA 817 0.12429598571128644 No Hit GTCCTACAGTGTGCATTTCTCATTT 754 0.11471135033820071 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 705 0.0 16.951551 2 CTGTAGG 705 0.0 15.962023 1 TAGGACC 345 0.0 15.466114 4 TTTAGGA 465 0.0 14.894748 2 GTCGAGG 35 0.004245639 14.723169 19 CCCCTAC 55 0.004820235 14.442644 1 CTTTAGG 505 0.0 13.894492 1 GTAGGAC 815 0.0 13.715812 3 TGTCCAA 75 1.4611487E-6 13.428237 10 CTCTAGG 80 2.8083485E-4 13.239092 1 GTGTAAA 90 5.1121777E-5 13.239091 1 GTATCAA 350 0.0 13.239091 1 GCCTTAT 70 0.0015486048 13.23909 1 GCTCTAA 60 0.008577053 13.23909 1 TAGGACG 805 0.0 12.85897 4 AGGACCT 590 0.0 12.809783 5 TTAGGAC 510 0.0 12.768003 3 GATATAC 135 9.458563E-8 12.748754 1 AGGACGT 780 0.0 12.742291 5 GGACCTG 585 0.0 12.7409935 6 >>END_MODULE