FastQCFastQC Report
Thu 26 May 2016
SRR1926673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657302
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA26330.40057690376721816No Hit
GTCCTACAGTGGACATTTCTAAATT25270.38445037440932783No Hit
CTTTAGGACGTGAAATATGGCGAGG24610.3744093278279999No Hit
GTCCTAAAGTGTGTATTTCTCATTT23870.36315118469135954No Hit
GTCCTACAGTGTGCATTTCTCATTT16800.2555902766156196No Hit
CTGTAGGACCTGGAATATGGCGAGA15390.23413894982823724No Hit
CTGAAGGACCTGGAATATGGCGAGA12650.192453392808785No Hit
GAATATGGCAAGAAAACTGAAAATC8350.1270344529607395No Hit
GTCCTTCAGTGTGCATTTCTCATTT7780.11836264000413813No Hit
GGAATATGGCGAGAAAACTGAAAAT7550.11486348740761476No Hit
CTGTAGGACATGGAATATGGCAAGA6790.10330107013214625No Hit
CCCATGTACTCTGCGTTGATACCAC6730.10238824771566189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTCC250.005913785719.063266
GACAGTC456.7568116E-414.7772447
TAGGACC5750.013.2613984
GGCGAGG7800.012.638267519
GCCATAT4100.012.3176071
TAGACTG701.055762E-412.2549525
TGTAGGA15850.012.0902032
CTGTAGG16100.012.0736511
TAGGACA4050.012.0027944
AAATGTC2150.011.9298027
AATGTCC2250.011.8217968
CCTAGAC657.782159E-411.7330313
TCTAGGT657.782159E-411.7330313
GTAGGAC16000.011.6780343
AGGACGT15200.011.5382895
CCTACAC1251.7318598E-811.4397073
TAAGAGT752.0073695E-411.4379574
GGACGTG14950.011.3486976
GTTCATA1104.761314E-711.2612081
TGTAGAA3400.011.2162562
Produced by FastQC (version 0.11.5)