##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926673_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 657302 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.744479706436312 31.0 27.0 31.0 16.0 34.0 2 28.35676599188805 31.0 28.0 33.0 18.0 34.0 3 28.725070059120466 31.0 28.0 33.0 23.0 34.0 4 32.438906317035396 35.0 32.0 37.0 26.0 37.0 5 32.01214358088063 35.0 32.0 37.0 22.0 37.0 6 32.12969989441687 35.0 32.0 37.0 22.0 37.0 7 31.973745705931215 35.0 32.0 37.0 18.0 37.0 8 31.92199476039933 35.0 32.0 37.0 18.0 37.0 9 33.12296174361253 37.0 33.0 39.0 17.0 39.0 10 32.96029678899502 37.0 32.0 39.0 17.0 39.0 11 33.16372687136202 37.0 33.0 39.0 18.0 39.0 12 32.9652077735957 37.0 33.0 39.0 17.0 39.0 13 33.087670507620544 37.0 33.0 39.0 17.0 39.0 14 33.67707994194449 38.0 33.0 40.0 17.0 41.0 15 33.821149182567524 38.0 33.0 40.0 17.0 41.0 16 33.80706737542256 38.0 33.0 40.0 17.0 41.0 17 33.72793480013753 38.0 33.0 40.0 17.0 41.0 18 33.693141052362535 38.0 33.0 40.0 16.0 41.0 19 33.711455312778604 39.0 33.0 40.0 11.0 41.0 20 33.70310146629708 39.0 33.0 40.0 10.0 41.0 21 33.62298304280224 39.0 33.0 40.0 10.0 41.0 22 33.545262299521376 39.0 33.0 40.0 10.0 41.0 23 33.170673449951465 38.0 33.0 40.0 2.0 41.0 24 33.25781299919976 38.0 33.0 40.0 2.0 41.0 25 33.048458699349766 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1676656855013725 1101 2 0.9403123821735022 1101 3 0.9299906216279616 1101 4 0.7677495540114592 1101 5 0.8423765951778357 1101 6 0.6654125167322746 1101 7 0.8328603288656673 1101 8 0.8862479437279767 1101 9 1.194500703347181 1101 10 1.2239391671886608 1101 11 0.9729431488251805 1101 12 0.9364002675527772 1101 13 0.9214898065116515 1101 14 1.2544578725660784 1101 15 0.977147284064749 1101 16 1.066854556107188 1101 17 0.873729210943786 1101 18 1.0551079541401762 1101 19 1.12546154702396 1101 20 0.9731604079467786 1101 21 0.8876103547495688 1101 22 0.8570368654079559 1101 23 1.1298282821858407 1101 24 1.269365153312279 1101 25 1.2709267222880314 1102 1 1.0155947730364154 1102 2 0.9928298975172503 1102 3 0.8832596406370996 1102 4 0.7663179095506365 1102 5 0.789738435637954 1102 6 0.5718818241176038 1102 7 0.662248962573802 1102 8 0.8066637201867088 1102 9 1.1881657862845856 1102 10 1.0468663902224904 1102 11 0.7973319945746269 1102 12 0.9684082695532794 1102 13 0.976046574358584 1102 14 1.1496641302708568 1102 15 1.2396127542334625 1102 16 1.106854322130566 1102 17 1.0677382395167214 1102 18 1.004778851396651 1102 19 1.3213774850728015 1102 20 1.066701337038701 1102 21 1.0356619875408057 1102 22 1.0143218006768606 1102 23 1.5748905711498935 1102 24 1.4541803629626457 1102 25 1.4680015757967269 1103 1 0.5879117559794835 1103 2 0.6080880399405011 1103 3 0.49380529797433326 1103 4 0.24673735997548363 1103 5 0.31131121920357785 1103 6 -1.500249643768825E-4 1103 7 0.425738941784946 1103 8 0.37054518448610096 1103 9 0.8062389775545995 1103 10 0.9147124001629265 1103 11 0.7701072653962484 1103 12 0.6824130820203678 1103 13 0.6244439369074044 1103 14 1.122700094908332 1103 15 1.0351532606460125 1103 16 0.7891041097613822 1103 17 0.923937456990906 1103 18 0.9909998501353954 1103 19 1.0885830917673047 1103 20 1.0854468517349432 1103 21 1.1594893282491086 1103 22 1.0248643474628807 1103 23 1.2575284370747681 1103 24 0.9442706652267603 1103 25 1.394393343797006 1104 1 0.5240272246607702 1104 2 0.6229460779618492 1104 3 0.5512676095504467 1104 4 0.3622045826608087 1104 5 0.21408307141521377 1104 6 0.09905963214043112 1104 7 0.1689916363677746 1104 8 0.45055454870384537 1104 9 0.6897536514442564 1104 10 0.8125527227470997 1104 11 0.5802317059316451 1104 12 0.7610192721767106 1104 13 0.6018100975124838 1104 14 0.9526899503391064 1104 15 0.7128294468714245 1104 16 0.7362932764408114 1104 17 0.6375747189928234 1104 18 0.711119134516629 1104 19 0.655848295311408 1104 20 0.7674725858305464 1104 21 0.7159849520817758 1104 22 0.5091045294379271 1104 23 0.8184618541317903 1104 24 0.7287988479486103 1104 25 0.9162989766948755 1105 1 1.087151123662533 1105 2 0.8947719228939803 1105 3 1.0191096780971556 1105 4 0.674184375556635 1105 5 0.8366366775272489 1105 6 0.25657882724036796 1105 7 0.6735146464691759 1105 8 0.6872516937979363 1105 9 1.1442184793676873 1105 10 1.2246804267127374 1105 11 0.690581394085207 1105 12 1.063305620264579 1105 13 1.1687325076527486 1105 14 1.347507829009011 1105 15 1.4825119076871758 1105 16 1.2184532512608044 1105 17 1.194835948009775 1105 18 1.2877249518740612 1105 19 1.3415476680336837 1105 20 1.5044117085594522 1105 21 1.423762722689844 1105 22 1.5061821565801523 1105 23 1.8605932074786509 1105 24 1.6804907914197642 1105 25 1.7658290382037976 1106 1 0.9197567484217473 1106 2 1.0584280304951221 1106 3 0.7711520366632065 1106 4 0.5175405185788904 1106 5 0.8455808603509745 1106 6 -0.5629689679719974 1106 7 -0.4008823832379811 1106 8 0.01842636220132121 1106 9 0.15031415587559138 1106 10 0.08496390848074498 1106 11 -0.157387254705327 1106 12 0.10461072584347875 1106 13 -0.18372516694439156 1106 14 0.06523616462613546 1106 15 0.03589970391560371 1106 16 -0.029668794922116604 1106 17 0.03856817571529092 1106 18 0.09245656288536708 1106 19 0.054108896454678757 1106 20 0.008954359949413515 1106 21 -0.062245233653285936 1106 22 -0.2717147891606402 1106 23 0.2408296398532599 1106 24 0.2964490594263154 1106 25 0.23493152939438744 1107 1 0.9090202704687051 1107 2 1.1197638140358421 1107 3 1.0545182403416007 1107 4 0.9711183399968988 1107 5 1.061642053859888 1107 6 0.8236907181267199 1107 7 0.9864157865355985 1107 8 1.060158384084982 1107 9 1.106122815424321 1107 10 0.6569198271753507 1107 11 0.5429781738216519 1107 12 0.8448263665208415 1107 13 0.6782228880983681 1107 14 1.1443009086929976 1107 15 1.1054432616784027 1107 16 1.0177254768070014 1107 17 1.3249971386243686 1107 18 1.1337673677672768 1107 19 1.2373319844729096 1107 20 1.048242482190652 1107 21 1.1424360922060757 1107 22 1.2140822781597933 1107 23 1.5619750136850996 1107 24 1.3539497357564443 1107 25 1.6098188661114747 1108 1 1.4894684715865765 1108 2 1.2156489216596178 1108 3 1.1486137755446073 1108 4 1.031065151684274 1108 5 0.0341028740278837 1108 6 0.598959909306032 1108 7 1.0881572295057147 1108 8 1.1292582228337587 1108 9 1.5144629114724353 1108 10 1.5039236810444976 1108 11 1.1996247806953804 1108 12 1.2810351710485293 1108 13 1.2726693818503207 1108 14 1.5721696171725696 1108 15 1.3480908732983252 1108 16 1.4070723566484915 1108 17 1.3111815271168652 1108 18 1.3994025886884813 1108 19 1.315036104283628 1108 20 1.413661647548686 1108 21 1.4140639566988327 1108 22 1.486653760933308 1108 23 1.8739978909831052 1108 24 1.6011053554846058 1108 25 1.594940190971208 1109 1 0.445598160772807 1109 2 -0.519050429033971 1109 3 0.5175302477061763 1109 4 0.7184888286014512 1109 5 -0.48038372215085445 1109 6 0.03733244841320982 1109 7 0.5738312568905961 1109 8 0.6493221854314122 1109 9 1.0326022595845714 1109 10 -1.522186969947029 1109 11 0.0029985926008819774 1109 12 0.636928227306214 1109 13 0.6772105828699964 1109 14 0.9833594864149475 1109 15 0.8699819289321624 1109 16 0.9340775518168769 1109 17 1.1202481536276423 1109 18 1.114397587128856 1109 19 1.0581215825510455 1109 20 1.2409970532128156 1109 21 1.0862477559480652 1109 22 1.1289601432102216 1109 23 1.2961617915542547 1109 24 1.2818796471095908 1109 25 1.3713270929800814 1110 1 0.5847048067285812 1110 2 -0.3865037462588319 1110 3 -0.007585494085923017 1110 4 0.400546352802003 1110 5 0.7530399942431494 1110 6 0.37087395043736393 1110 7 0.6519898950585876 1110 8 0.7408376837796844 1110 9 1.2611540716362555 1110 10 0.9148688737644619 1110 11 0.4414169950159419 1110 12 0.7743975318865282 1110 13 0.6074521191095172 1110 14 0.6882754468220185 1110 15 0.8075479751804338 1110 16 0.4437630732680944 1110 17 0.6852157095909561 1110 18 0.8995334342315999 1110 19 0.5009077425881046 1110 20 0.7781721965085779 1110 21 0.7753595493478329 1110 22 0.6209611666432053 1110 23 0.9104350481603944 1110 24 0.8462847691191513 1110 25 0.9457333958738516 1111 1 0.4256942060218698 1111 2 0.6850510240591916 1111 3 0.7618491348893421 1111 4 0.5215710877843378 1111 5 0.8298951179180598 1111 6 0.41769373842323176 1111 7 0.7765481989455161 1111 8 0.8168094152284517 1111 9 0.9987865804701741 1111 10 1.0553282377220583 1111 11 0.5792261822950948 1111 12 0.6577897580349372 1111 13 0.6136358646925544 1111 14 0.8764379909916329 1111 15 0.6935903780072863 1111 16 0.8077206704412419 1111 17 0.7249683597676295 1111 18 0.7316889112634044 1111 19 0.6828865411746747 1111 20 0.635062655872531 1111 21 0.9555715634821027 1111 22 1.054706749682076 1111 23 1.2567248008105665 1111 24 1.0750833556915893 1111 25 0.814107954177743 1112 1 0.3341074047914745 1112 2 0.19979223462430795 1112 3 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1.1242398581530821 2203 1 -0.09266992334601554 2203 2 0.12568277219425283 2203 3 -0.13305172887194416 2203 4 -0.2716813906216302 2203 5 -0.03461153206230705 2203 6 -0.13755508372046776 2203 7 0.06917187385717583 2203 8 0.1447074325036759 2203 9 0.2895667875765753 2203 10 0.3694600594731341 2203 11 0.30990197734809755 2203 12 0.25769283027679535 2203 13 0.1614740076057899 2203 14 0.5950775669633614 2203 15 0.4097852886865212 2203 16 0.3533349961185053 2203 17 0.48490958964591613 2203 18 0.6233660068660711 2203 19 0.5226183334793504 2203 20 0.323120910682114 2203 21 0.6792814427518366 2203 22 0.6179030345269894 2203 23 0.9168304397976144 2203 24 0.8496772406542945 2203 25 0.7941549457364374 2204 1 0.12808984595718798 2204 2 0.17899903867259326 2204 3 0.14183056867984334 2204 4 0.0454825688887297 2204 5 0.20210868921829572 2204 6 -0.015485874680919665 2204 7 0.2799769586029406 2204 8 0.32322673570518035 2204 9 0.7773875267479511 2204 10 0.6264398146520449 2204 11 0.6297112993819951 2204 12 0.5647490920470375 2204 13 0.49962137107094406 2204 14 0.8031284144209891 2204 15 0.7531968941479121 2204 16 0.7986651514857428 2204 17 0.400973031265508 2204 18 0.41178114340091554 2204 19 0.5616072694492189 2204 20 0.6104384718873845 2204 21 0.5582097619608746 2204 22 0.5643042821729409 2204 23 0.5898265556168241 2204 24 0.32219960411945436 2204 25 0.4504078882976401 2205 1 0.9511701455762918 2205 2 0.828931780130759 2205 3 0.8867373271013221 2205 4 0.8624756164794576 2205 5 0.9097089044456261 2205 6 0.9595888176997533 2205 7 0.7373527426959363 2205 8 0.809416079120215 2205 9 1.208837620909911 2205 10 1.2143587645692122 2205 11 0.885116054206911 2205 12 1.206484079232517 2205 13 1.065896522225671 2205 14 1.4684067231872575 2205 15 1.4583637661301267 2205 16 1.3835602989589404 2205 17 1.232044353639239 2205 18 1.3168510214859594 2205 19 1.4472606684835227 2205 20 1.5850126191198726 2205 21 1.4451870922923504 2205 22 1.3240107517366795 2205 23 1.611934084325597 2205 24 1.4120336525172803 2205 25 1.6034111591867273 2206 1 0.8386844740330908 2206 2 1.0644430388978314 2206 3 0.9678849636345142 2206 4 0.9176037468829819 2206 5 1.1524183913411612 2206 6 0.9229031111854056 2206 7 1.1150605781761094 2206 8 1.271841522573812 2206 9 1.3161650480047768 2206 10 1.3870288117802616 2206 11 1.231102143461051 2206 12 1.3499143603616943 2206 13 1.0307408034714172 2206 14 1.4484828825779772 2206 15 1.2035600902738182 2206 16 1.3708201151661257 2206 17 1.4168523037470635 2206 18 1.3510936046881454 2206 19 1.4198763161297734 2206 20 1.6715370697814151 2206 21 1.5786360247008915 2206 22 1.3848459793405752 2206 23 1.7273145898529805 2206 24 1.866940298978399 2206 25 1.7312598506829104 2207 1 -0.9599527158941363 2207 2 0.1067776240143381 2207 3 0.3074976326679817 2207 4 0.41667773125753627 2207 5 0.336456161381566 2207 6 0.4499413428089767 2207 7 0.310059523942936 2207 8 0.24232869227116183 2207 9 0.7855481221487111 2207 10 0.6778803472566324 2207 11 0.6471125262331725 2207 12 0.5387075767986147 2207 13 0.2481008896336121 2207 14 -4.134553650875404 2207 15 -1.3319704169547961 2207 16 -0.3818753026236337 2207 17 -0.1982143978707569 2207 18 0.34820728521560795 2207 19 0.506326285642487 2207 20 0.7073553541736288 2207 21 0.6493223589896857 2207 22 0.7228573536617304 2207 23 0.8178319049205385 2207 24 0.6072142856323737 2207 25 0.7351405684732413 2208 1 0.6899755940743297 2208 2 1.039200886419362 2208 3 0.9395236389410364 2208 4 0.8438757727053812 2208 5 1.0136378304704117 2208 6 0.9727295764474206 2208 7 1.148731175238165 2208 8 1.23785381304344 2208 9 1.3194974277516778 2208 10 1.2976152404461487 2208 11 1.0806930009061801 2208 12 1.1890141927643825 2208 13 1.119031661726197 2208 14 1.3474130665571877 2208 15 1.331794974804403 2208 16 1.328607047973371 2208 17 0.9780462389373739 2208 18 1.2770174815027744 2208 19 0.9063524814168886 2208 20 0.9968688233998648 2208 21 1.3292378636833249 2208 22 1.3385576795761835 2208 23 1.6689395820938486 2208 24 1.427475045059019 2208 25 1.394709062759631 2209 1 -0.4988026185412089 2209 2 -0.017499472550493778 2209 3 0.2128566795364648 2209 4 0.3743161694384298 2209 5 0.6366769439136668 2209 6 0.47083455591917556 2209 7 0.7161313333166355 2209 8 0.762012237308479 2209 9 0.9161386719609581 2209 10 0.5591184678815466 2209 11 0.5886181935643151 2209 12 0.8024094218683828 2209 13 0.5444507343325142 2209 14 0.9861248642606597 2209 15 0.7720929088066413 2209 16 0.6839233970194059 2209 17 0.7921777953516269 2209 18 1.0446544828420485 2209 19 1.0054918319778494 2209 20 0.7788735383097389 2209 21 0.5741997986076868 2209 22 0.823866810803267 2209 23 0.8100596064642787 2209 24 0.9028397781918258 2209 25 1.0507943976883851 2210 1 -0.5313475180919234 2210 2 -0.6393837114405478 2210 3 -0.07109005789864398 2210 4 0.253828676131171 2210 5 0.2901690988593124 2210 6 0.278169507149471 2210 7 0.4753411245031707 2210 8 0.4673011017543196 2210 9 0.8203793952423766 2210 10 0.6351523506014729 2210 11 0.3330770419475826 2210 12 0.06659948402356264 2210 13 0.08457370614153348 2210 14 0.3649957409513007 2210 15 0.3713956999698951 2210 16 0.48239265115726937 2210 17 0.4406925892152529 2210 18 0.45008462228381774 2210 19 0.5134532817997624 2210 20 0.5283723103375806 2210 21 0.6058600926226347 2210 22 0.718025504380563 2210 23 0.6773884178337894 2210 24 0.5756273913864192 2210 25 0.5463822820844157 2211 1 -1.6687935516798404 2211 2 -0.8244628520061212 2211 3 -0.026424674448168872 2211 4 0.07752682584328596 2211 5 0.44303661403551686 2211 6 0.2666281905635586 2211 7 0.28490482337001666 2211 8 0.28965195075975103 2211 9 0.630941744519788 2211 10 0.46150008998194636 2211 11 0.16810060445173036 2211 12 0.5492749211143106 2211 13 0.4079525776284214 2211 14 0.46742034818568357 2211 15 0.4332197132018223 2211 16 0.4768241642614086 2211 17 0.49452739916269906 2211 18 0.49848979512510283 2211 19 0.3141481410770268 2211 20 0.41673097612842724 2211 21 0.7378947989667708 2211 22 0.6572131110450457 2211 23 0.8879770112472727 2211 24 0.546651271261517 2211 25 0.8973213110056051 2212 1 0.9905450016565993 2212 2 0.14549616066503646 2212 3 0.4070927782880851 2212 4 0.5022885385620199 2212 5 0.569730705577495 2212 6 0.6794819968690575 2212 7 0.6810603969051563 2212 8 0.5612140437715745 2212 9 0.884179943477065 2212 10 0.6546363163979834 2212 11 0.6114953451750011 2212 12 0.43784171446201015 2212 13 0.27969615143352655 2212 14 0.22924972124369702 2212 15 0.46306822090142674 2212 16 -0.12397225145126356 2212 17 0.44099806435063016 2212 18 0.30320212973635563 2212 19 0.2858686339915266 2212 20 0.5706988478620048 2212 21 0.6184007787505834 2212 22 0.6815013738705673 2212 23 0.7945668971510145 2212 24 0.8974766422430847 2212 25 0.17045907059271315 2213 1 0.3495721843031774 2213 2 0.16504406876530453 2213 3 -0.49640896593096073 2213 4 -1.0206124201303695 2213 5 -2.2866078022568352 2213 6 -0.8678036000409399 2213 7 -0.6022549312862466 2213 8 -0.4322687461972805 2213 9 -1.1436186929707368 2213 10 -0.7304513318885526 2213 11 -0.9280122165193632 2213 12 -0.9604441215860504 2213 13 -0.6228257780849518 2213 14 -7.795006508209735 2213 15 -3.4053137263151285 2213 16 -2.163410491022937 2213 17 -1.4178965222894604 2213 18 -5.82521780042044 2213 19 -3.388324833352172 2213 20 -5.833474250874101 2213 21 -2.754486175942528 2213 22 -2.2572643945861017 2213 23 -4.838648355949175 2213 24 -2.4579132225834286 2213 25 -1.985531523930561 2214 1 -0.0033834872446050213 2214 2 -0.21709811805280665 2214 3 -1.9918911416257927 2214 4 -2.1881651491910254 2214 5 -0.7014853598336188 2214 6 -0.9424032598943128 2214 7 -0.6483048432557474 2214 8 -0.7985930784263466 2214 9 -6.188653001260576 2214 10 -2.7692469318604545 2214 11 -1.6182484883096429 2214 12 -2.188200731358183 2214 13 -2.2020745307463017 2214 14 -3.5078036903125422 2214 15 -3.058705002026194 2214 16 -2.1841025523875075 2214 17 -2.5722072532592684 2214 18 -1.8581178325466787 2214 19 -5.285465598094834 2214 20 -3.210313881065396 2214 21 -4.621719600127484 2214 22 -6.895350223460333 2214 23 -7.304135678751088 2214 24 -6.86855334077114 2214 25 -4.908526426337033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28203.0 3 19.0 4 539.0 5 3443.0 6 3831.0 7 3121.0 8 2343.0 9 1934.0 10 1689.0 11 1528.0 12 1519.0 13 1426.0 14 1398.0 15 1435.0 16 1530.0 17 1586.0 18 1787.0 19 2016.0 20 2682.0 21 3437.0 22 4445.0 23 5459.0 24 6431.0 25 7450.0 26 8589.0 27 9408.0 28 10935.0 29 13205.0 30 15881.0 31 19757.0 32 25370.0 33 32243.0 34 41382.0 35 54118.0 36 71813.0 37 105588.0 38 159762.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.53855570646381 20.34733937913771 9.197976746593906 30.91612816780457 2 12.646044972035156 21.5319407982346 41.15983715709775 24.6621770726325 3 18.10858975158919 25.987791756772594 28.825881110399227 27.077737381238997 4 11.883228252152223 18.460503628762872 37.71250214956073 31.943765969524172 5 12.511488760796391 39.605486606083105 35.44914207108119 12.433882562039308 6 30.915526941096704 38.8289587011099 18.022222357964438 12.233291999828964 7 27.621733975794925 31.51871458918117 22.49499410264147 18.364557332382443 8 23.590125007239507 40.64701381755222 19.82893425308098 15.933926922127286 9 26.37597794228597 15.535660123426872 19.988507796865402 38.09985413742175 10 15.304203337837343 30.101705437680316 33.89511740861118 20.69897381587116 11 33.73853430845278 22.098109349492688 25.96217481861044 18.201181523444095 12 24.58491877903058 24.8043074423268 31.847261673469944 18.763512105172673 13 30.00553778932667 23.16819361572002 25.185531156150446 21.640737438802866 14 19.8967738006425 24.339306648802545 27.136158097471107 28.627761453083846 15 21.51850781238114 34.688493663375375 22.456602108658277 21.336396415585206 16 20.525615941918737 28.45671442872661 30.49737861448441 20.52029101487024 17 20.388238730761508 30.763028012585274 28.528046056755457 20.320687199897762 18 20.269262836312297 27.97759236606844 32.10645147548366 19.646693322135604 19 23.871698009867746 26.838715762985455 26.791248347388457 22.498337879758342 20 23.921794746963315 28.614353350697403 27.99773617974246 19.46611572259682 21 21.843721521798994 27.201845121184963 26.563781281330773 24.390652075685264 22 23.26525706600619 30.476402019163185 26.132584413252964 20.12575650157766 23 21.091370481148697 30.026836979044642 28.05346705167488 20.82832548813179 24 23.82751228892787 27.28590793535362 28.835583197296817 20.050996578421692 25 21.89651316520133 28.92403936704797 28.516463538013788 20.662983929736907 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 103.0 1 103.0 2 101.5 3 100.0 4 100.0 5 100.0 6 159.0 7 218.0 8 218.0 9 218.0 10 388.5 11 559.0 12 559.0 13 559.0 14 1130.0 15 1701.0 16 1701.0 17 1701.0 18 3677.0 19 5653.0 20 5653.0 21 5653.0 22 10209.5 23 14766.0 24 14766.0 25 14766.0 26 23609.5 27 32453.0 28 32453.0 29 32453.0 30 45845.5 31 59238.0 32 59238.0 33 59238.0 34 66762.5 35 74287.0 36 74287.0 37 74287.0 38 78534.0 39 82781.0 40 82781.0 41 82781.0 42 85357.5 43 87934.0 44 87934.0 45 87934.0 46 89087.5 47 90241.0 48 90241.0 49 90241.0 50 83640.0 51 77039.0 52 77039.0 53 77039.0 54 65181.0 55 53323.0 56 53323.0 57 53323.0 58 44526.5 59 35730.0 60 35730.0 61 35730.0 62 28601.5 63 21473.0 64 21473.0 65 21473.0 66 16486.5 67 11500.0 68 11500.0 69 11500.0 70 8377.0 71 5254.0 72 5254.0 73 5254.0 74 3563.0 75 1872.0 76 1872.0 77 1872.0 78 1312.0 79 752.0 80 752.0 81 752.0 82 495.0 83 238.0 84 238.0 85 238.0 86 153.5 87 69.0 88 69.0 89 69.0 90 42.5 91 16.0 92 16.0 93 16.0 94 9.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0818497433447639 2 0.03453511475699146 3 0.031188099229882155 4 0.029058180258085323 5 0.020994915579140183 6 0.3751700131750702 7 0.1636994866895278 8 0.17952174190858997 9 0.18332516864394144 10 0.016735077635546523 11 0.0022820560412108895 12 0.003194878457695245 13 0.0 14 0.07485143815171717 15 0.0018256448329687115 16 0.0024341931106249483 17 0.003955563804765542 18 0.003955563804765542 19 0.0013692336247265336 20 9.128224164843558E-4 21 1.5213706941405927E-4 22 0.0 23 0.0 24 4.564112082421779E-4 25 1.5213706941405927E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 657302.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.7599711143628 #Duplication Level Percentage of deduplicated Percentage of total 1 92.31816001882692 72.70975616413932 2 5.555948174552282 8.75172635481269 3 0.9509983641723162 2.247018110760537 4 0.3569451663848526 1.1245196397552966 5 0.20381502326253034 0.8026232672415037 6 0.12467941079613422 0.5891848075715583 7 0.08210741052584986 0.4526744096903745 8 0.06364303731651397 0.4010019024543164 9 0.040127572790910566 0.28444018265114457 >10 0.2480683069384205 3.6310862462827593 >50 0.027753885744121968 1.5058190938052314 >100 0.022901595702318863 3.573982043987249 >500 0.003493463750535239 1.7109439722117754 >1k 0.0013585692363192595 2.2152238046362056 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2633 0.40057690376721816 No Hit GTCCTACAGTGGACATTTCTAAATT 2527 0.38445037440932783 No Hit CTTTAGGACGTGAAATATGGCGAGG 2461 0.3744093278279999 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2387 0.36315118469135954 No Hit GTCCTACAGTGTGCATTTCTCATTT 1680 0.2555902766156196 No Hit CTGTAGGACCTGGAATATGGCGAGA 1539 0.23413894982823724 No Hit CTGAAGGACCTGGAATATGGCGAGA 1265 0.192453392808785 No Hit GAATATGGCAAGAAAACTGAAAATC 835 0.1270344529607395 No Hit GTCCTTCAGTGTGCATTTCTCATTT 778 0.11836264000413813 No Hit GGAATATGGCGAGAAAACTGAAAAT 755 0.11486348740761476 No Hit CTGTAGGACATGGAATATGGCAAGA 679 0.10330107013214625 No Hit CCCATGTACTCTGCGTTGATACCAC 673 0.10238824771566189 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCTCC 25 0.0059137857 19.06326 6 GACAGTC 45 6.7568116E-4 14.777244 7 TAGGACC 575 0.0 13.261398 4 GGCGAGG 780 0.0 12.6382675 19 GCCATAT 410 0.0 12.317607 1 TAGACTG 70 1.055762E-4 12.254952 5 TGTAGGA 1585 0.0 12.090203 2 CTGTAGG 1610 0.0 12.073651 1 TAGGACA 405 0.0 12.002794 4 AAATGTC 215 0.0 11.929802 7 AATGTCC 225 0.0 11.821796 8 CCTAGAC 65 7.782159E-4 11.733031 3 TCTAGGT 65 7.782159E-4 11.733031 3 GTAGGAC 1600 0.0 11.678034 3 AGGACGT 1520 0.0 11.538289 5 CCTACAC 125 1.7318598E-8 11.439707 3 TAAGAGT 75 2.0073695E-4 11.437957 4 GGACGTG 1495 0.0 11.348697 6 GTTCATA 110 4.761314E-7 11.261208 1 TGTAGAA 340 0.0 11.216256 2 >>END_MODULE